Evolutionary Engineering of Industrially Important Microbial Phenotypes

The Neutral Theory of Molecular Evolution

1979 • 7,965 citations

Methods for general and molecular bacteriology

1994 • 6,549 citations

The Neutral Theory of Molecular Evolution

1983 • 6,141 citations

The Origins of Order

1993 • 5,064 citations

DNA Repair and Mutagenesis

2006 • 4,725 citations

The origins of order

1993 • 2,842 citations

The relation of recombination to mutational advance

1964 • 2,547 citations

DNA repair and mutagenesis

1996 • 2,289 citations

Over-production of Proteins inEscherichia coli: Mutant Hosts that Allow Synthesis of some Membrane Proteins and Globular Proteins at High Levels

1996 • 1,892 citations

How clonal are bacteria?

1993 • 1,804 citations

Systematic functional analysis of the yeast genome

1998 • 1,213 citations

The "hitchhiking effect" revisited.

1989 • 1,137 citations

Toward a Science of Metabolic Engineering

1991 • 1,137 citations

Dynamics of adaptation and diversification: a 10,000-generation experiment with bacterial populations.

1994 • 1,007 citations

Metabolic Flux Balancing: Basic Concepts, Scientific and Practical Use

1994 • 914 citations

Evolution of high mutation rates in experimental populations of E. coli

1997 • 909 citations

THE POPULATION GENETICS OF ADAPTATION: THE DISTRIBUTION OF FACTORS FIXED DURING ADAPTIVE EVOLUTION

1998 • 819 citations

MANUAL OF INDUSTRIAL MICROBIOLOGY AND BIOTECHNOLOGY

1999 • 798 citations

Role of mutator alleles in adaptive evolution

1997 • 697 citations

Design by Directed Evolution

1998 • 668 citations

Quantitative phenotypic analysis of yeast deletion mutants using a highly parallel molecular bar–coding strategy

1996 • 570 citations

Reductive evolution of resident genomes

1998 • 514 citations

Improvement of microbial strains and fermentation processes

2000 • 504 citations

Systematic changes in gene expression patterns following adaptive evolution in yeast

1999 • 471 citations

Directed evolution of biocatalysts

1999 • 386 citations

Diminishing Returns from Mutation Supply Rate in Asexual Populations

1999 • 384 citations

Metabolic Flux Ratio Analysis of Genetic and Environmental Modulations of Escherichia coli Central Carbon Metabolism

1999 • 375 citations

Directed evolution of enzyme catalysts

1997 • 370 citations

Microbial evolution in a simple unstructured environment: genetic differentiation in Escherichia coli.

1994 • 370 citations

The Population Genetics of Adaptation: The Distribution of Factors Fixed during Adaptive Evolution

1998 • 364 citations

Biosynthetically Directed Fractional 13C-labeling of Proteinogenic Amino Acids. An Efficient Analytical Tool to Investigate Intermediary Metabolism

1995 • 349 citations

Evolution of microbial diversity during prolonged starvation

1999 • 336 citations

Methods for Intense Aeration, Growth, Storage, and Replication of Bacterial Strains in Microtiter Plates

2000 • 336 citations

Mathematical Modeling and Analysis in Biochemical Engineering: Past Accomplishments and Future Opportunities

1998 • 326 citations

Surfaces of Selective Value Revisited

1988 • 324 citations

Selection in chemostats

1983 • 323 citations

Biosynthetically Directed Fractional 13C-labeling of Proteinogenic Amino Acids. An Efficient Analytical Tool to Investigate Intermediary Metabolism

1995 • 320 citations

Inhibition and Activation of Bacterial Luciferase Synthesis

1972 • 308 citations

Multiple duplications of yeast hexose transport genes in response to selection in a glucose-limited environment

1998 • 308 citations

TRANSLATIONAL ACCURACY AND THE FITNESS OF BACTERIA

1992 • 305 citations

Evolution of Escherichia coli During Growth in a Constant Environment

1987 • 284 citations

Isolation of a thermostable enzyme variant by cloning and selection in a thermophile.

1986 • 279 citations

Molecular Genetics of Bacteria

1998 • 276 citations

Genetic variation: molecular mechanisms and impact on microbial evolution

2000 • 276 citations

Repeated evolution of an acetate-crossfeeding polymorphism in long-term populations of Escherichia coli

1998 • 271 citations

Protein identification in the post-genome era: the rapid rise of proteomics

1997 • 270 citations

DNA chips: promising toys have become powerful tools

1999 • 265 citations

Genome-wide hypermutation in a subpopulation of stationary-phase cells underlies recombination-dependent adaptive mutation

1997 • 265 citations

Pathway engineering for the production of aromatic compounds in Escherichia coli

1996 • 265 citations

SPONTANEOUS MUTATORS IN BACTERIA: Insights into Pathways of Mutagenesis and Repair

1996 • 254 citations

Frequency of fixation of adaptive mutations is higher in evolving diploid than haploid yeast populations

1983 • 246 citations

Genomic evolution during a 10,000-generation experiment with bacteria

1999 • 245 citations

Experimental Studies of Natural Selection in Bacteria

1990 • 234 citations

GC‐MS Analysis of Amino Acids Rapidly Provides Rich Information for Isotopomer Balancing

2000 • 231 citations

Isotopomer Analysis Using GC-MS

1999 • 223 citations

¹³C-NMR, MS and metabolic flux balancing in biotechnology research

1998 • 220 citations

Mimicking Somatic Hypermutation: Affinity Maturation of Antibodies Displayed on Bacteriophage Using a Bacterial Mutator Strain

1996 • 216 citations

Sensing Starvation: a Homoserine Lactone—Dependent Signaling Pathway in Escherichia coli

1994 • 215 citations

Microbial Selection

1952 • 204 citations

Inverse metabolic engineering: A strategy for directed genetic engineering of useful phenotypes

1996 • 193 citations

Relative fitness can decrease in evolving asexual populations of S. cerevisiae

1983 • 187 citations

Microbial Selection in Continuous Culture

1977 • 182 citations

Physiology and metabolic fluxes of wild-type and riboflavin-producing Bacillus subtilis

1996 • 181 citations

High-Throughput Isolation of Caenorhabditis elegans Deletion Mutants

1999 • 179 citations

Genetic analysis of a plasmid-encoded, host genotype-specific enhancement of bacterial fitness

1994 • 168 citations

Industrial strain improvement: Mutagenesis and random screening procedures

1984 • 158 citations

Bacterial growth inhibition by overproduction of protein

1996 • 158 citations

Untitled

1998 • 157 citations

Does diploidy increase the rate of adaptation?

1994 • 155 citations

Character Change, Speciation, and the Higher Taxa

1982 • 152 citations

Role of cryptic genes in microbial evolution.

1984 • 152 citations

Enzyme activity and fitness: Evolution in solution

1990 • 146 citations

The Evolution of Metabolic Function

1992 • 145 citations

The evolutionary genetics of adaptation: a simulation study

1999 • 143 citations

Enzymes of evolutionary change

1999 • 140 citations

SPONTANEOUS MUTATION

1991 • 131 citations

Evolution of novel metabolic pathways for the degradation of chloroaromatic compounds.

1997 • 111 citations

Protein evolution by molecular breeding

1999 • 105 citations

Isolation of ethanol-tolerant mutants of yeast by continuous selection

1982 • 102 citations

Saccharomyces cerevisiae pol30 (Proliferating Cell Nuclear Antigen) Mutations Impair Replication Fidelity and Mismatch Repair

1999 • 98 citations

Bioenergetic consequences of microbial adaptation to low-nutrient environments

1997 • 86 citations

Novel approaches for discovering industrial enzymes

1999 • 83 citations

Genetic interactions between error-prone and error-free postreplication repair pathways in Saccharomyces cerevisiae

1999 • 81 citations

Mini-transposons in microbial ecology and environmental biotechnology

1998 • 73 citations

Bacterial response to acetate challenge: a comparison of tolerance among species

2000 • 69 citations

Protein engineering from a bioindustrial point of view

1997 • 68 citations

NMR in microbiology: theory and applications.

2000 • 60 citations

Use of a glycerol-limited, long-term chemostat for isolation of Escherichia coli mutants with improved physiological properties

1997 • 58 citations

Variable selection and multivariate methods for the identification of microorganisms by flow cytometry

1999 • 56 citations

PHYSIOLOGICAL CHARACTERIZATION OF ADAPTIVE CLONES IN EVOLVING POPULATIONS OF THE YEAST, SACCHAROMYCES CEREVISIAE

1985 • 55 citations

Direct Selection for Mutators in Escherichia coli

1999 • 55 citations

Mathematical models of metabolic pathways

1999 • 54 citations

Construction and Analysis of a Library for Random Insertional Mutagenesis in Streptococcus pneumoniae : Use for Recovery of Mutants Defective in Genetic Transformation and for Identification of Essential Genes

1999 • 54 citations

Functional Genomic Technologies: Creating New Paradigms for Fundamental and Applied Biology

1999 • 53 citations

Improvement of antibiotic titers fromStreptomyces bacteria by interactive continuous selection

1996 • 52 citations

Identification of adaptive changes in an evolving population of Escherichia coli: the role of changes with regulatory and highly pleiotropic effects.

1991 • 49 citations

Evolutionary Approaches to Protein Engineering

1999 • 48 citations

Glucose Catabolism ofEscherichia coliStrains with Increased Activity and Altered Regulation of Key Glycolytic Enzymes

1999 • 46 citations

Probing the properties of C60 through fulleroids ABC61

1993 • 43 citations

Environmentally directed mutations and their impact on industrial biotransformation and fermentation processes

2000 • 42 citations

[45] Chemostats used for studying natural selection and adaptive evolution

1993 • 42 citations

Rapid screening for metabolite overproduction in fermentor broths, using pyrolysis mass spectrometry with multivariate calibration and artificial neural networks

1994 • 42 citations

Developments in metabolic engineering

1997 • 41 citations

Metabolic Consequences of Phosphotransferase (PTS) Mutation in a Phenylalanine-Producing Recombinant Escherichia coli

1997 • 40 citations

Plasmid stability in budding yeast populations: Steady‐state growth with selection pressure

1984 • 40 citations

Biotechnology in the 21st century

2000 • 38 citations

Expression and secretion of Interferon-α1 by Streptomyces lividans: use of staphylokinase signals and amplification of a neo gene

1988 • 36 citations

Enzyme evolution in Rhodobacter sphaeroides: selection of a mutant expressing a new galactitol dehydrogenase and biochemical characterization of the enzyme

1995 • 34 citations

Selection of a subtilisin-hyperproducing Bacillus in a highly structured environment

1998 • 33 citations

Whole genome scan for habitat‐specific genes by signature‐tagged mutagenesis (minireview)

1998 • 33 citations

Isolation of acetic acid-tolerant Baker’s yeast variants in a turbidostat

1991 • 33 citations

Binding-protein-dependent Sugar Transport by Agrobacterium radiobacter and A. tumefaciens Grown in Continuous Culture

1989 • 31 citations

The Isolation of Strains of Bacillus subtilis Showing Improved Plasmid Stability Characteristics by Means of Selective Chemostat Culture

1988 • 29 citations

Pleiotropic Effects of Adaptation to a Single Carbon Source for Growth on Alternative Substrates

1999 • 29 citations

Use of a series of chemostat cultures to isolate 'improved' variants of the Quorne myco-protein fungus, Fusarium graminearum A3/5

1994 • 26 citations

Physiology of Amidase Production by Methylophilus methylotrophus: Isolation of Hyperactive Strains Using Continuous Culture

1989 • 26 citations

Development of morphological heterogeneity in glucose-limited chemostat cultures of Aspergillus oryzae

1994 • 24 citations

Continuous Cultivation of Microorganisms

1984 • 23 citations

Rapid in vivo evolution of a β-lactamase using phagemids

2000 • 23 citations

Auxostats for continuous culture research

1994 • 23 citations

Analysis of a continuous-culture technique for the selection of mutants tolerant to extreme environmental stress

1999 • 23 citations

Periodic selection and hitchhiking in a bacterial population

1995 • 22 citations

Mini-transposons in microbial ecology and environmental biotechnology

1998 • 21 citations

AnEscherichia coli host strain useful for efficient overproduction of secreted recombinant protein

1998 • 21 citations

Increased Phenylalanine Production by Growing and Nongrowing Escherichia coli Strain CWML2

1998 • 21 citations

Production of protease with Bacillus licheniformis mutants insensitive to repression of exoenzyme biosynthesis

1994 • 19 citations

Co-overexpression of RspAB Improves Recombinant Protein Production in Escherichia coli

2000 • 18 citations

Toward an understanding of evolutionary potential

1999 • 17 citations

pH oscillations and constant low pH delay the appearance of highly branched (colonial) mutants in chemostat cultures of the quorn® myco-protein fungus, Fusarium graminearum A3/5

2000 • 17 citations

Genetics Society of America 1994 Records, Proceedings and Reports

1994 • 17 citations

Chemostat Selection of anEscherichia coliMutant Containing Permease with Enhanced Lactose Affinity

1996 • 16 citations

[44] Acquisition of new metabolic activities by microbial populations

1993 • 15 citations

Evolutionary Biotechnology — Reflections and Perspectives

1999 • 15 citations

Stability of recombinant protein production byPenicillium chrysogenumin prolonged chemostat culture

1995 • 11 citations

Use of Continuous Culture for the Selection of Plasmids with Improved Segregational Stability

1995 • 11 citations

Chemostat selection of Escherichia coli mutants secreting thymidine, cytosine, uracil, guanine, and thymine

1994 • 10 citations

Increased mutagenesis mediated by cloned plasmid CAM-OCT genes: potential for expanding substrate ranges of Pseudomonas spp

1996 • 9 citations

Biomass, microorganisms for special applications, microbial products I, energy from renewable resources

1983 • 8 citations

Selection of Microbial Mutants Tolerant To Extreme Environmental Stress Using Continuous Culture-Control Design

1999 • 7 citations

Selection of an adhesive phenotype of Streptococcus salivarius subsp. thermophilus for use in fixed-bed reactors

1996 • 7 citations

Enzyme beauty

1999 • 6 citations

The isolation of strains of Saccharomyces cerevisiae showing altered plasmid stability characteristics by means of selective continuous culture

1999 • 4 citations