The CXXC motif: imperatives for the formation of native disulfide bonds in the cell.

MOLSCRIPT: a program to produce both detailed and schematic plots of protein structures

1991 • 14,222 citations

Rapid and efficient site-specific mutagenesis without phenotypic selection.

1985 • 7,573 citations

Site-directed mutagenesis by overlap extension using the polymerase chain reaction

1989 • 7,496 citations

Current protocols in molecular biology

1988 • 4,543 citations

Protein folding in the cell

1992 • 4,138 citations

Getting started with yeast

2002 • 3,496 citations

Guide to Yeast Genetics and Molecular Biology

1993 • 2,377 citations

Oxidized Redox State of Glutathione in the Endoplasmic Reticulum

1992 • 1,909 citations

THIOREDOXIN

1985 • 1,885 citations

Identification of a protein required for disulfide bond formation in vivo

1991 • 967 citations

Oxidation-reduction potentials of organic systems

1960 • 877 citations

Polymer support oligonucleotide synthesis XVIII^1.2): use ofβ-cyanoethyi-N,N-dialkylamino-/N-morpholino phosphoramidite of deoxynucleosides for the synthesis of DNA fragments simplifying deprotection and isolation of the final product

1984 • 840 citations

Molecular and Cellular Aspects of Thiol–Disulfide Exchange

1990 • 763 citations

A simple and efficient procedure for transformation of yeasts.

1992 • 637 citations

Sequence of protein disulphide isomerase and implications of its relationship to thioredoxin

1985 • 622 citations

Rat liver thioredoxin and thioredoxin reductase: purification and characterization

1982 • 610 citations

[20] In vitro mutagenesis and plasmid shuffling: From cloned gene to mutant yeast

1991 • 584 citations

Crystal structure of thioredoxin from Escherichia coli at 1.68 Å resolution

1990 • 568 citations

The role of the α-helix dipole in protein function and structure

1985 • 517 citations

Reexamination of the Folding of BPTI: Predominance of Native Intermediates

1991 • 505 citations

Alpha-factor-directed synthesis and secretion of mature foreign proteins in Saccharomyces cerevisiae.

1984 • 477 citations

Hydrogen donor system for Escherichia coli ribonucleoside-diphosphate reductase dependent upon glutathione.

1976 • 474 citations

Isolation of Viral IgY Antibodies from Yolks of Immunized Hens

1980 • 449 citations

Acceleration of Reactivation of Reduced Bovine Pancreatic Ribonuclease by a Microsomal System from Rat Liver

1963 • 439 citations

Molecular cloning of the beta-subunit of human prolyl 4-hydroxylase. This subunit and protein disulphide isomerase are products of the same gene.

1987 • 420 citations

A pathway for disulfide bond formation in vivo.

1993 • 397 citations

Crystal structure of the DsbA protein required for disulphide bond formation in vivo

1993 • 387 citations

Protein disulfide isomerase is a component of the microsomal triglyceride transfer protein complex.

1990 • 369 citations

Structural and functional relations among thioredoxins of different species

1991 • 363 citations

Enzymatic Synthesis of Deoxyribonucleotides

1964 • 340 citations

Isotope effects on enzyme-catalyzed reactions

1992 • 323 citations

Enzymatic Synthesis of Deoxyribonucleotides

1963 • 314 citations

Sorting of soluble ER proteins in yeast.

1988 • 270 citations

Protein disulfide isomerase exhibits chaperone and anti-chaperone activity in the oxidative refolding of lysozyme.

1994 • 267 citations

High-resolution solution structures of oxidized and reduced Escherichia coli thioredoxin

1994 • 261 citations

Mimicking the active site of protein disulfide-isomerase by substitution of proline 34 in Escherichia coli thioredoxin

1991 • 256 citations

Redox properties of protein disulfide isomerase (dsba) from escherichia coli

1993 • 242 citations

The Essential Function of Protein-disulfide Isomerase Is to Unscramble Non-native Disulfide Bonds

1995 • 205 citations

The essential function of yeast protein disulfide isomerase does not reside in its isomerase activity

1993 • 201 citations

Conformational restrictions on the pathway of folding and unfolding of the pancreatic trypsin inhibitor

1977 • 188 citations

Determination of the reduction-oxidation potential of the thioredoxin-like domains of protein disulfide-isomerase from the equilibrium with glutathione and thioredoxin

1993 • 188 citations

Tryptophan Fluorescence Study of Conformational Transitions of the Oxidized and Reduced Form of Thioredoxin

1972 • 164 citations

Three-dimensional solution structure of the reduced form of Escherichia coli thioredoxin determined by nuclear magnetic resonance spectroscopy

1990 • 160 citations

The enzymic reactivation of reduced ribonuclease

1963 • 158 citations

Protein disulfide isomerase is both an enzyme and a chaperone

1993 • 157 citations

The yeast EUG1 gene encodes an endoplasmic reticulum protein that is functionally related to protein disulfide isomerase.

1992 • 150 citations

Evolutionary optimization of the catalytic effectiveness of an enzyme

1989 • 145 citations

The reactivities and ionization properties of the active-site dithiol groups of mammalian protein disulphide-isomerase

1991 • 144 citations

High-resolution three-dimensional structure of reduced recombinant human thioredoxin in solution

1991 • 141 citations

Catalysis of the oxidative folding of ribonuclease A by protein disulfide isomerase: pre-steady-state kinetics and the utilization of the oxidizing equivalents of the isomerase

1991 • 136 citations

Peptide binding to protein disulfide isomerase occurs at a site distinct from the active sites.

1993 • 129 citations

Oxidation and Disulfide Interchange in the Reactivation of Reduced Ribonuclease

1964 • 124 citations

Engineering ribonuclease A: production, purification and characterization of wild-type enzyme and mutants at Gln11

1995 • 121 citations

Enzymatic Synthesis of Deoxyribonucleotides

1964 • 116 citations

Thioredoxin: A Multifunctional Regulatory Protein with a Bright Future in Technology and Medicine

1994 • 115 citations

Urea dependence of thiol-disulfide equilibria in thioredoxin: confirmation of the linkage relationship and a sensitive assay for structure

1989 • 115 citations

A Pro to His mutation in active site of thioredoxin increases its disulfide-isomerase activity 10-fold. New refolding systems for reduced or randomly oxidized ribonuclease.

1992 • 110 citations

Relationship between electrostatics and redox function in human thioredoxin: characterization of pH titration shifts using two-dimensional homo- and heteronuclear NMR

1992 • 109 citations

The role of the thiol/disulfide centers and peptide binding site in the chaperone and anti-chaperone activities of protein disulfide isomerase.

1994 • 104 citations

A conformational study of thioredoxin and its tryptic fragments.

1981 • 96 citations

Enzyme kinetics and molecular evolution

1989 • 95 citations

Catalytic Mechanism of DsbA and Its Comparison with That of Protein Disulfide Isomerase

1995 • 95 citations

[8] Maintaining protein stability

1990 • 87 citations

Proton Sharing between Cysteine Thiols in Escherichia coli Thioredoxin: Implications for the Mechanism of Protein Disulfide Reduction

1995 • 82 citations

A glycosylated protoxin in killer yeast: Models for its structure and maturation

1983 • 80 citations

Efficient Catalysis of Disulfide Formation During Protein Folding with a Single Active-site Cysteine

1995 • 74 citations

Cloning and nucleotide sequence of the trxA gene of Escherichia coli K-12

1985 • 72 citations

Proton-transfer effects in the active-site region of Escherichia coli thioredoxin using two-dimensional proton NMR

1991 • 64 citations

A putative glutathione-binding site in T4 glutaredoxin investigated by site-directed mutagenesis

1991 • 57 citations

<scp>III</scp>. Yeast sequencing reports. Determination of the sequence of the yeast YCL313 gene localized on chromosome III. Homology with the protein disulfide isomerase (PDI gene product) of other organisms

1991 • 54 citations

Functional replacement of the Saccharomyces cerevisiae Trg1/Pdi1 protein by members of the mammalian protein disulfide isomerase family.

1993 • 50 citations

Constitutive Overexpression of Secreted Heterologous Proteins Decreases Extractable BiP and Protein Disulfide Isomerase Levels in Saccharomyces cerevisiae

1995 • 47 citations

Nucleotide sequence of the thioredoxin gene from Escherichia coli

1984 • 39 citations

The Yeast EUG1 Gene Encodes an Endoplasmic Reticulum Protein That Is Functionally Related to Protein Disulfide Isomerase

1992 • 35 citations

The Saccharomyces cerevisiae ACP2 gene encodes an essential HMG1-like protein.

1988 • 34 citations

The enzymic reactivation of reduced ribonuclease

1963 • 33 citations

Evaluating the effects of a single amino acid substitution on both the native and denatured states of a protein.

1991 • 25 citations

Characterization by ¹H NMR of a C32S,C35S double mutant of Escherichia coli thioredoxin confirms its resemblance to the reduced wild‐type protein

1994 • 22 citations

Production of Rat Protein Disulfide Isomerase in Saccharomyces cerevisiae

1995 • 17 citations

Determination of for aliphatic ketones from nuclear magnetic resonance chemical shift data

1970 • 16 citations

Enzyme relaxation in the reaction catalyzed by triosephosphate isomerase: detection and kinetic characterization of two unliganded forms of the enzyme

1987 • 15 citations

The Saccharomyces cerevisiae ACP2 Gene Encodes an Essential HMG1-Like Protein

1988 • 8 citations