The essential function of yeast protein disulfide isomerase does not reside in its isomerase activity

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase

1988 • 17,059 citations

PCR protocols — A guide to methods and applications

1990 • 10,078 citations

“Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A

1981 • 8,657 citations

Transformation of intact yeast cells treated with alkali cations

1983 • 7,688 citations

Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase

1988 • 6,107 citations

Protein folding in the cell

1992 • 4,138 citations

Getting started with yeast

2002 • 3,496 citations

[1] Getting started with yeast

1991 • 2,456 citations

Oxidized Redox State of Glutathione in the Endoplasmic Reticulum

1992 • 1,909 citations

THIOREDOXIN

1985 • 1,885 citations

THE KINETICS OF FORMATION OF NATIVE RIBONUCLEASE DURING OXIDATION OF THE REDUCED POLYPEPTIDE CHAIN

1961 • 1,148 citations

Protein Oligomerization in the Endoplasmic Reticulum

1989 • 993 citations

Protein degradation in the endoplasmic reticulum

1990 • 659 citations

Sequence of protein disulphide isomerase and implications of its relationship to thioredoxin

1985 • 622 citations

[20] In vitro mutagenesis and plasmid shuffling: From cloned gene to mutant yeast

1991 • 584 citations

ERD2, a yeast gene required for the receptor-mediated retrieval of luminal ER proteins from the secretory pathway

1990 • 548 citations

A Critical Evaluation of the Nitrogen Assimilation Tests Commonly Used in the Classification of Yeasts

1946 • 530 citations

Acceleration of Reactivation of Reduced Bovine Pancreatic Ribonuclease by a Microsomal System from Rat Liver

1963 • 439 citations

Defective co-translational formation of disulphide bonds in protein disulphide-isomerase-deficient microsomes

1988 • 330 citations

Protein disulfide isomerase. A multifunctional protein resident in the lumen of the endoplasmic reticulum.

1992 • 318 citations

Lyticase: Endoglucanase and Protease Activities That Act Together in Yeast Cell Lysis

1980 • 310 citations

Catalysis by protein-disulphide isomerase of the unfolding and refolding of proteins with disulphide bonds

1980 • 173 citations

Role of primary structure and disulfide bond formation in beta-lactamase secretion

1983 • 170 citations

ERD1, a yeast gene required for the retention of luminal endoplasmic reticulum proteins, affects glycoprotein processing in the Golgi apparatus.

1990 • 160 citations

DISULFIDE INTERCHANGE AND THE THREE-DIMENSIONAL STRUCTURE OF PROTEINS

1965 • 152 citations

The yeast EUG1 gene encodes an endoplasmic reticulum protein that is functionally related to protein disulfide isomerase.

1992 • 150 citations

The reactivities and ionization properties of the active-site dithiol groups of mammalian protein disulphide-isomerase

1991 • 144 citations

Expression and site-directed mutagenesis of human protein disulfide isomerase in Escherichia coli. This multifunctional polypeptide has two independently acting catalytic sites for the isomerase activity.

1992 • 144 citations

Glycosylation site binding protein and protein disulfide isomerase are identical and essential for cell viability in yeast.

1991 • 137 citations

Peptide binding to protein disulfide isomerase occurs at a site distinct from the active sites.

1993 • 129 citations

Conformational changes associated with proteolytic processing of presecretory proteins allow glutathione-catalyzed formation of native disulfide bonds.

1982 • 125 citations

Protein disulfide isomerase is essential for viability in Saccharomyces cerevisiae

1991 • 123 citations

In vivo cross-linking of protein disulfide isomerase to immunoglobulins

1987 • 113 citations

[34a] Carboxymethylation

1972 • 105 citations

[45] Analysis of polypeptide transit through yeast secretory pathway

1991 • 96 citations

Renaturation of the reduced bovine pancreatic trypsin inhibitor

1974 • 95 citations

Formation and intracellular transport of a heterodimeric viral spike protein complex.

1991 • 89 citations

Production of Leukemia Inhibitory Factor in Escherichia coli by a Novel Procedure and Its Use in Maintaining Embryonic Stem Cells in Culture

1989 • 84 citations

Purification and Characterization of Yeast Protein Disulfide Isomerase

1990 • 79 citations

Peptide binding by protein disulfide isomerase, a resident protein of the endoplasmic reticulum lumen.

1991 • 78 citations

Interchain disulfide bond formation in types I and II procollagen. Evidence for a protein disulfide isomerase catalyzing bond formation.

1987 • 71 citations

Defective folding and stable association with protein disulfide isomerase/prolyl hydroxylase of type I procollagen with a deletion in the pro alpha 2(I) chain that preserves the Gly-X-Y repeat pattern.

1992 • 67 citations

Role of protein disulphide-isomerase in the expression of native proteins.

1989 • 66 citations

Molecular Structure of a Yeast Gene, PDI1, Encoding Protein Disulfide Isomerase That Is Essential for Cell Growth

1991 • 54 citations

<scp>III</scp>. Yeast sequencing reports. Determination of the sequence of the yeast YCL313 gene localized on chromosome III. Homology with the protein disulfide isomerase (PDI gene product) of other organisms

1991 • 54 citations

Functional replacement of the Saccharomyces cerevisiae Trg1/Pdi1 protein by members of the mammalian protein disulfide isomerase family.

1993 • 50 citations

The Saccharomyces cerevisiae TRG1 gene is essential for growth and encodes a lumenal endoplasmic reticulum glycoprotein involved in the maturation of vacuolar carboxypeptidase.

1991 • 43 citations

Active site-directed photoaffinity labeling and partial characterization of oligosaccharyltransferase.

1985 • 36 citations

Conserved residues flanking the thiol/disulfide centers of protein disulfide isomerase are not essential for catalysis of thiol/disulfide exchange

1992 • 29 citations

Chemical modification of essential residues in purified rat liver protein disulphide-isomerase

1984 • 4 citations

Incorporation of ‘acid’ β-glucosidase into phosphatidylcholine membranes

1984 • 3 citations