Isotope effects on enzyme-catalyzed reactions

Hydrogen Tunneling in Enzyme Reactions

1989 • 455 citations

[29] Solvent isotope effects on enzyme systems

1982 • 394 citations

Condensed phase isotope effects

1974 • 288 citations

Low-barrier hydrogen bonds and low fractionation factor bases in enzymic reactions

1992 • 250 citations

Human immunodeficiency virus-1 protease. 2. Use of pH rate studies and solvent kinetic isotope effects to elucidate details of chemical mechanism

1991 • 242 citations

Simulations of quantum mechanical corrections for rate constants of hydride-transfer reactions in enzymes and solutions

1991 • 202 citations

Mechanistic studies of peptidyl prolyl cis-trans isomerase: evidence for catalysis by distortion

1990 • 164 citations

Structures and isotopic fractionation factors of complexes, A1HA2-

1980 • 158 citations

Solvent and secondary kinetic isotope effects for the microhydrated SN2 reaction of Cl-(H2O)n with CH3Cl

1991 • 135 citations

Transition-state analysis of nucleoside hydrolase from Crithidia fasciculata

1991 • 135 citations

Structures and isotopic fractionation factors of complexes AHA-1

1977 • 133 citations

Nucleoside hydrolase from Crithidia fasciculata. Metabolic role, purification, specificity, and kinetic mechanism.

1991 • 130 citations

Evidence that both protium and deuterium undergo significant tunneling in the reaction catalyzed by bovine serum amine oxidase

1989 • 130 citations

Calculations of isotope effects in elimination reactions. New experimental criteria for tunneling in slow proton transfers

1985 • 114 citations

Structure-function studies of substrate oxidation by bovine serum amine oxidase: relationship to cofactor structure and mechanism

1991 • 107 citations

PPIase catalysis by human FK506-binding protein proceeds through a conformational twist mechanism.

1992 • 105 citations

[30] Deuterium and tritium kinetic isotope effects on initial rates

1982 • 100 citations

Transition-state structures for enzymic and alkaline phosphotriester hydrolysis

1991 • 97 citations

Calculation of H/D carbon-12/carbon-13, and carbon-12/carbon-14 fractionation factors from valence force fields derived for a series of simple organic molecules

1972 • 95 citations

Mechanistic studies of enzymic and nonenzymic prolyl cis-trans isomerization

1992 • 83 citations

Catalytic power of pyruvate decarboxylase. Rate-limiting events and microscopic rate constants from primary carbon and secondary hydrogen isotope effects

1991 • 78 citations

Crotonase-catalyzed .beta.-elimination is concerted: a double isotope effect study

1991 • 72 citations

Inactivation of dopamine .beta.-hydroxylase by p-cresol: isolation and characterization of covalently modified active site peptides

1988 • 67 citations

.alpha.-Deuterium and carbon-13 isotope effects for a simple, intermolecular sulfur-to-oxygen methyl-transfer reaction. Transition-state structures and isotope effects in transmethylation and transalkylation

1979 • 65 citations

Oxidation-reduction of general acyl-CoA dehydrogenase by the butyryl-CoA/crotonyl-CoA couple. A new investigation of the rapid reaction kinetics

1988 • 64 citations

Tunneling paths in intramolecular proton transfer

1991 • 59 citations

The rate of formation of transition-state analogs in the active site of adenosine deaminase is encounter-controlled: implications for the mechanism

1992 • 57 citations

Investigation of the enzymic mechanism of yeast orotidine-5'-monophosphate decarboxylase using carbon-13 kinetic isotope effects

1991 • 55 citations

Glutathione reductase: comparison of steady-state and rapid reaction primary kinetic isotope effects exhibited by the yeast, spinach, and Escherichia coli enzymes

1990 • 54 citations

Contributions of thiolate "desolvation" to catalysis by glutathione S-transferase isozymes 1-1 and 2-2: evidence from kinetic solvent isotope effects

1991 • 54 citations

Orotidine-5'-monophosphate decarboxylase catalysis: kinetic isotope effects and the state of hybridization of a bound transition-state analog

1990 • 54 citations

Secondary hydrogen/deuterium isotope effects in methyl-transfer reactions decrease with increasing looseness of the transition structure

1991 • 51 citations

Leaving group dependence and proton inventory studies of the phosphorylation of a cytoplasmic phosphotyrosyl protein phosphatase from bovine heart

1991 • 50 citations

Phosphoryl-transfer reactions of phosphodiesters: characterization of transition states by heavy-atom isotope effects

1991 • 48 citations

Electron transfer within xanthine oxidase: a solvent kinetic isotope effect study

1991 • 46 citations

Catalytic mechanism of an active-site mutant (D38N) of .DELTA.5-3-ketosteroid isomerase

1991 • 40 citations

Secondary oxygen-18 isotope effects for hexokinase-catalyzed phosphoryl transfer from ATP

1991 • 40 citations

Transition-state analysis of a Vmax mutant of AMP nucleosidase by the application of heavy-atom kinetic isotope effects

1991 • 39 citations

Origin of apparent swain–schaad deviations in criteria for tunneling

1991 • 39 citations

Studies of the catalytic mechanism of an active-site mutant (Y14F) of .DELTA.5-3-ketosteroid isomerase by kinetic deuterium isotope effects

1991 • 37 citations

Multiple isotope effects with alternative dinucleotide substrates as a probe of the malic enzyme reaction

1991 • 36 citations

Nitrogen and deuterium isotope effects on quaternization of N,N-dimethyl-p-toluidine

1991 • 34 citations

Secondary nitrogen-15 isotope effects on the reactions catalyzed by alcohol and formate dehydrogenases

1991 • 30 citations

Human erythrocyte glutathione reductase: chemical mechanism and structure of the transition state for hydride transfer

1991 • 28 citations

Exponential relationships among multiple hydrogen isotope effects as probes of hydrogen tunneling

1992 • 26 citations

Nitrogen-15 isotope effects on nonenzymic and aspartate transcarbamylase catalyzed reactions of carbamyl phosphate

1992 • 24 citations

Slow step after bond-breaking by porcine pepsin identified using solvent deuterium isotope effects

1991 • 23 citations

The .alpha.- and .beta.-deuterium kinetic isotope effects on the inactivation of the general acyl-coenzyme A dehydrogenase from pig kidney by (2-methylenecyclopropane)acetyl-CoA

1992 • 22 citations

Carbon-13 isotope effects as a probe of the kinetic mechanism and allosteric properties of Escherichia coli aspartate transcarbamylase

1992 • 21 citations

Carbon-11/carbon-14 kinetic isotope effects in enzyme mechanism studies. 11C/14C Kinetic isotope effect of the tyrosine phenol-lyase catalyzed .alpha.,.beta.-elimination of L-tyrosine

1992 • 20 citations

Carbon-13 and deuterium isotope effects on the reaction catalyzed by glyceraldehyde-3-phosphate dehydrogenase

1991 • 19 citations

Carbon-13 and nitrogen-15 isotope effects as a probe of the chemical mechanism of Escherichia coli aspartate transcarbamylase

1992 • 16 citations

Solvent modulation of the dynamics of hydride transfer

1991 • 15 citations

Interfacial catalysis by phospholipase A2: evaluation of the interfacial rate constants by steady-state isotope effect studies

1991 • 14 citations

Effect of commitments to catalysis on the degree of curvature in proton inventories of the kinetic parameters for enzyme-catalyzed reactions: application to tryptophan indole-lyase

1991 • 14 citations

Carbon-13 isotope effect studies of Escherichia coli aspartate transcarbamylase in the presence of the bisubstrate analog N-(phosphonoacetyl)-L-aspartate

1992 • 12 citations

Theoretical kinetic isotope effects for the hydride transfer from formate to carbon dioxide: A comparison of theory with experiment

1991 • 11 citations

Mechanism of inhibition of dopamine .beta.-monooxygenase by quinol and phenol derivatives, as determined by solvent and substrate deuterium isotope effects

1991 • 9 citations

Characterization of transition states by isotopic mapping and structure-reactivity coefficients: vibrational analysis calculations for the general-base-catalyzed addition of alcohols to acetaldehyde

1991 • 7 citations

β-deuterium kinetic isotope effects in the purine nucleoside phosphorylase reaction

1991 • 7 citations