Protein and Gene Structure and Regulation of NADPH-Cytochrome P450 Oxidoreductase

Cloned and expressed nitric oxide synthase structurally resembles cytochrome P-450 reductase

1991 • 2,371 citations

Hepatic Triphosphopyridine Nucleotide-Cytochrome c Reductase: Isolation, Characterization, and Kinetic Studies

1962 • 1,431 citations

Microsomal Triphosphopyridine Nucleotide-Cytochrome c Reductase of Liver

1962 • 909 citations

Redesign of the coenzyme specificity of a dehydrogenase by protein engineering

1990 • 727 citations

A general mechanism for microsomal activation of quinone anticancer agents to free radicals.

1978 • 645 citations

Role of Hemoprotein P-450 in Fatty Acid ω-Hydroxylation in a Soluble Enzyme System from Liver Microsomes

1968 • 625 citations

2 Evolutionary and Structural Relationships among Dehydrogenases

1975 • 607 citations

Atomic Structure of Ferredoxin-NADP ⁺ Reductase: Prototype for a Structurally Novel Flavoenzyme Family

1991 • 532 citations

NADPH cytochrome P-450 reductase activation of quinone anticancer agents to free radicals.

1979 • 492 citations

Interaction of pyrophosphate moieties with .alpha.-helixes in dinucleotide-binding proteins

1985 • 475 citations

Properties of hepatic reduced nicotinamide adenine dinucleotide phosphate-cytochrome c reductase

1973 • 344 citations

Microsomal Electron Transport

1973 • 327 citations

The catalytic mechanism of glutathione reductase as derived from x-ray diffraction analyses of reaction intermediates.

1983 • 323 citations

Regulation of three forms of cytochrome P-450 and epoxide hydrolase in rat liver microsomes. Effects of age, sex, and induction.

1981 • 304 citations

Separate roles for FMN and FAD in catalysis by liver microsomal NADPH-cytochrome P-450 reductase.

1981 • 295 citations

Comparison of 2,3,7,8-tetrachlorodibenzo-p-dioxin, a potent inducer of aryl hydrocarbon hydroxylase, with 3-methylcholanthrene.

1974 • 293 citations

Structural analysis of the FMN binding domain of NADPH-cytochrome P-450 oxidoreductase by site-directed mutagenesis

1989 • 261 citations

Immunochemical Evidence for an Association of Heme Oxygenase with the Microsomal Electron Transport System

1972 • 250 citations

The Structure of the Oxidized Form of Clostridial Flavodoxin at 1.9-A Resolution

1974 • 244 citations

Coding Nucleotide, 5′ Regulatory, and Deduced Amino Acid Sequences of P-450BM-3, a Single Peptide Cytochrome P-450:NADPH-P-450 Reductase from Bacillus megaterium

1989 • 242 citations

NADPH-cytochrome P-450 oxidoreductase: flavin mononucleotide and flavin adenine dinucleotide domains evolved from different flavoproteins

1986 • 241 citations

Identification of the high and low potential flavins of liver microsomal NADPH-cytochrome P-450 reductase.

1978 • 238 citations

Transcriptional regulation of rat liver epoxide hydratase, NADPH-Cytochrome P-450 oxidoreductase, and cytochrome P-450b genes by phenobarbital.

1983 • 222 citations

Studies on the Mechanism of Microsomal Triphosphopyridine Nucleotide-Cytochrome c Reductase

1965 • 201 citations

Cytochrome b5 reduction by NADPH-cytochrome P-450 reductase.

1979 • 199 citations

cDNA sequence of adrenodoxin reductase

1989 • 195 citations

Redox properties of the reduced nicotinamide adenine dinucleotide phosphate-cytochrome P-450 and reduced nicotinamide adenine dinucleotide-cytochrome b₅ reductases

1974 • 193 citations

Role of NADPH:cytochrome c reductase and DT-diaphorase in the biotransformation of mitomycin C1.

1984 • 178 citations

Structural features of liver microsomal NADPH-cytochrome P-450 reductase. Hydrophobic domain, hydrophilic domain, and connecting region.

1982 • 173 citations

Coding nucleotide sequence of rat NADPH-cytochrome P-450 oxidoreductase cDNA and identification of flavin-binding domains.

1985 • 171 citations

Characterization, localization and regulation of a novel phenobarbital-inducible form of cytochrome P₄₅₀, compared with three further P₄₅₀-isoenzymes, NADPH P₄₅₀-reductase, glutathione transferases and microsomal epoxide hydrolase

1984 • 152 citations

Characterization of the Flavoprotein Moieties of NADPH-Sulfite Reductase from Salmonella typhimurium and Escherichia coli

1989 • 149 citations

The Binding of Riboflavin-5′-Phosphate in a Flavoprotein: Flavodoxin at 2.0-Å Resolution

1973 • 145 citations

Biochemical Distinctions between the Nuclear and Microsomal Membranes from Rat Hepatocytes

1971 • 141 citations

Probing the role of lysines and arginines in the catalytic function of cytochrome P450d by site-directed mutagenesis. Interaction with NADPH-cytochrome P450 reductase

1991 • 137 citations

Induction of the hepatic mixed-function oxidase system by synthetic glucocorticoids. Transcriptional and post-transcriptional regulation.

1987 • 128 citations

An unusual yet strongly conserved flavoprotein reductase in bacteria and mammals

1991 • 126 citations

Chromosomal assignments of genes coding for components of the mixed-function oxidase system in mice. Genetic localization of the cytochrome P-450PCN and P-450PB gene families and the nadph-cytochrome P-450 oxidoreductase and epoxide hydratase genes.

1985 • 123 citations

Effect of modification of individual cytochrome c lysines on the reaction with cytochrome b₅

1977 • 117 citations

Amino acid sequence of spinach ferredoxin:NADP oxidoreductase

1984 • 114 citations

Modification and identification of cytochrome b5 carboxyl groups involved in protein-protein interaction with cytochrome b5 reductase.

1979 • 114 citations

Role of a hydrophobic polypeptide in the N-terminal region of NADPH-cytochrome P-450 reductase in complex formation with P-450lm

1979 • 113 citations

Effect of chronic ethanol feeding on the activities and submicrosomal distribution of reduced nicotinamide adenine dinucleotide phosphate-cytochrome P-450 reductase and the demethylases for aminopyrine and ethylmorphine

1973 • 110 citations

Microsomal lipid peroxidation: The role of NADPH — Cytochrome P450 reductase and cytochrome P450

1990 • 103 citations

Turnover of membrane proteins: Kinetics of induction and degradation of seven forms of rat liver microsomal cytochrome P-450, NADPH-cytochrome P-450 reductase, and epoxide hydrolase

1984 • 100 citations

Chapter 4 Structural and Thermodynamic Aspects of the Transfer of Proteins into and across Membranes

1985 • 96 citations

Primary Structure of Saccharomyces cerevisiae NADPH-Cytochrome P450 Reductase Deduced from Nucleotide Sequence of Its Cloned Gene1

1988 • 89 citations

Induction of microsomal NADPH-cytochrome P-450 reductase and cytochrome P-450IVA1 (P-450LA omega) by dehydroepiandrosterone in rats: a possible peroxisomal proliferator.

1989 • 89 citations

Studies on the microsomal mixed-function oxidase system: mechanism of action of hepatic NADPH-cytochrome P-450 reductase

1981 • 88 citations

Role of electrostatic interactions in the reaction of NADPH-cytochrome P-450 reductase with cytochromes P-450

1988 • 87 citations

NADPH-cytochrome P-450 oxidoreductase gene organization correlates with structural domains of the protein

1990 • 83 citations

Induction of a high affinity nitrosamine demethylase in rat liver microsomes by acetone and isopropanol

1983 • 78 citations

Localization of cytochrome c-binding domain on NADPH-cytochrome P-450 reductase.

1986 • 77 citations

Evidence for a new physiological role of hepatic NADPH:ferricytochrome (P-450) oxidoreductase. Direct electron input to the microsomal fatty acid chain elongation system.

1981 • 75 citations

Hypophysectomy differentially alters P-450 protein levels and enzyme activities in rat liver: pituitary control of hepatic NADPH cytochrome P-450 reductase.

1989 • 75 citations

Selective chemical modification of a functionally linked lysine in cytochrome P-450 LM2

1984 • 74 citations

Effect of microsomal preparations and induction on cytochrome P-450-dependent monooxygenases in fetal and neonatal rat liver

1979 • 71 citations

Preparation and characterization of FAD-dependent NADPH-cytochrome P-450 reductase.

1986 • 68 citations

NADPH: cytochrome P-450 reductase in olfactory epithelium Relevance to cytochrome P-450-dependent reactions

1986 • 65 citations

Structural and functional analysis of NADPH-cytochrome P-450 reductase from human liver: complete sequence of human enzyme and NADPH-binding sites

1989 • 64 citations

Differences in the mechanism of functional interaction between NADPH-cytochrome P-450 reductase and its redox partners.

1986 • 64 citations

Complete amino acid sequence of NADPH-cytochrome P-450 reductase from porcine hepatic microsomes

1986 • 64 citations

Disruption of the Saccharomyces Cerevlsiae gene for NADPH-cytochrome P450 reductase causes increased sensitivity to ketoconazole

1989 • 63 citations

Presence of NADPH-cytochrome P450 reductase in central catecholaminergic neurones

1984 • 59 citations

Isolation of the membrane-binding peptide of NADPH-cytochrome P-450 reductase. Characterization of the peptide and its role in the interaction of reductase with cytochrome P-450.

1981 • 56 citations

Amino acid sequence of Desulfovibrio vulgaris flavodoxin.

1977 • 55 citations

Quantitation of mRNAs specific for the mixed-function oxidase system in rat liver and extrahepatic tissues during development

1989 • 55 citations

Purification to homogeneity and enzymological characterization of a functional covalent complex composed of cytochromes P-450 isozyme 2 and b5 from rabbit liver.

1987 • 54 citations

Isolation and characterization of the alkane-inducible NADPH-cytochrome P-450 oxidoreductase gene from Candida tropicalis. Identification of invariant residues within similar amino acid sequences of divergent flavoproteins.

1990 • 52 citations

Disappearance of sex difference in rat liver drug metabolism in old age

1985 • 51 citations

Structural analysis of NADPH-cytochrome P-450 reductase from porcine hepatic microsomes. Sequences of proteolytic fragments, cysteine-containing peptides, and a NADPH-protected cysteine peptide.

1984 • 50 citations

Molecular Cloning and Sequence Analysis of Full-Length cDNA for Rabbit Liver NADPH-Cytochrome P-450 Reductase mRNA1

1986 • 50 citations

Effects of isoniazid treatment on selected hepatic mixed-function oxidases.

1979 • 49 citations

Evidence of binary complex formations between cytochrome P-450, cytochrome b5, and NADPH-cytochrome P-450 reductase of hepatic microsomes

1986 • 47 citations

NADPH-cytochrome P-450 oxidoreductase. The role of cysteine 566 in catalysis and cofactor binding.

1991 • 45 citations

Time-resolved fluorescence spectroscopy of NADPH-cytochrome P-450 reductase: demonstration of energy transfer between the two prosthetic groups

1989 • 44 citations

Isolation of a human cytochrome P‐450 reductase cDNA clone and localization of the corresponding gene to chromosome 7q11.2

1989 • 43 citations

Laser flash photolysis studies of the reduction kinetics of NADPH:cytochrome P-450 reductase

1991 • 37 citations

Electrostatic interactions between cytochrome P-450 LM2 and NADPH-cytochrome P-450 reductase.

1988 • 36 citations

Studies on the effect of chronic consumption of moderate amounts of ethanol on male rat hepatic microsomal drug-metabolizing activity

1985 • 35 citations

Isolation and sequencing of an active-site peptide from spinach ferredoxin-NADP+ oxidoreductase after affinity labeling with periodate-oxidized NADP+

1985 • 34 citations

Flavins of NADPH-cytochrome P-450 reductase: evidence for structural alteration of flavins in their one-electron-reduced semiquinone states from resonance Raman spectroscopy

1985 • 33 citations

Studies on FAD- and FMN-binding domains in NADPH-cytochrome P-450 reductase from rabbit liver microsomes.

1982 • 33 citations

NADPH:cytochrome P-450(c) reductase: Biochemical characterization in rat brain and cultured neurons and evolution of activity during development

1989 • 32 citations

Cytochrome b5, cytochrome c, and cytochrome P-450 interactions with NADPH-cytochrome P-450 reductase in phospholipid vesicles

1988 • 28 citations

Regulation of aromatase activity of rat granulosa cells: induction of synthesis of NADPH-cytochrome P-450 reductase by FSH and dibutyryl cyclic AMP

1985 • 28 citations

Localization of the free radical on the flavin mononucleotide of the air-stable semiquinone state on NADPH-cytochrome P-450 reductase using phosphorus-31 NMR spectroscopy

1986 • 27 citations

Immunochemical characterization of NADPH-cytochrome P-450 reductase from Jerusalem artichoke and other higher plants

1989 • 27 citations

Effects of 2-acetylaminofluorene and N-hydroxy-2-acetylaminofluorene on the cellular levels of epoxide hydratase, cytochrome P-450b, and NADPH-cytochrome c (P-450) oxidoreductase messenger ribonucleic acids.

1982 • 26 citations

Regulation of synthesis and activity of bovine adrenocortical NADPH-cytochrome P-450 reductase by ACTH

1985 • 21 citations

Structural comparison between the trout and mammalian hydrophilic domain of nadphcytochrome P-450 reductase

1987 • 21 citations

Microsomal electron-transport reductase activities and fatty acid elongation in rat brain. Developmental changes, regional distribution and comparison with liver activity

1983 • 20 citations

Complete structure of the hydrophilic domain in the porcine NADPH-cytochrome P-450 reductase

1986 • 20 citations

Differential effect of phenobarbital and β‐naphthoflavone on the mRNAs coding for cytochrome P450 and NADPH cytochrome P450 reductase

1982 • 19 citations

Differences in the spectral interactions between NADPH-cytochrome P-450 reductase and a series of cytochrome P-450 enzymes

1986 • 19 citations

Differential inducibility of nuclear envelope epoxide hydratase by trans-stilbene oxide and phenobarbital.

1982 • 19 citations

The Functional Role of Thiol Groups in Protease-Solubilized NADPH-Cytochrome c Reductase from Pork-Liver Microsomes

1977 • 19 citations

Structure predictions and surface charge of nitrogenase flavodoxins from Klebsiella pneumoniae and Azotobacter vinelandii

1986 • 17 citations

Chemical modification of NADPH‐cytochrome P‐450 reductase

1986 • 14 citations

Photochemically induced dynamic nuclear polarization study on microsomal NADPH-cytochrome P-450 reductase.

1984 • 14 citations

Biphasic response for hepatic microsomal enzyme induction by 2,3,7,8-tetrachlorodibenzo-p-dioxin in C57BL/6J and DBA/2J mice

1989 • 13 citations

A ³¹P‐nuclear‐magnetic‐resonance study of NADPH–cytochrome‐P‐450 reductase and of the Azotobacter flavodoxin/ferredoxin‐NADP⁺ reductase complex

1990 • 13 citations

The effect of dexamethasone, insulin and triiodothyronine on microsomal NADPH-cytochrome-c (P-450) reductase in primary cultures of isolated hepatocytes

1987 • 11 citations

STUDIES ON THE COSUBSTRATE SITE OF PROTEASE SOLUBILIZED NADPH-CYTOCHROME P450 REDUCTASE

1980 • 11 citations

Induction of xenobiotic-metabolizing enzymes and peroxisome proliferation in rat liver caused by dietary exposure to di(2-ethylhexyl)phosphate

1987 • 11 citations

Enzyme induction with high doses of rifampicin in Wistar rats

1983 • 10 citations

Levels of adrenodoxin, NADPH-cytochrome P-450 reductase and cytochromes P-45011β, P-45021, P-450scc, in adrenal zona fasciculata-reticularis tissue from androgen-treated rats

1988 • 10 citations

Effects of chronic acetone administration on ethanol-inducible monooxygenase activities in the rat

1988 • 10 citations

Effect of dietary protein, dichlorodiphenyltrichloroethane (DDT) and hexachlorocyclohexane (HCH) on hepatic microsomal enzyme activity in rats

1985 • 9 citations

Increase in renal cytochrome P-450 and NADPH cytochrome c reductase activity following drug inhibition of hepatic monooxygenase activity

1987 • 7 citations

The cosubstrate NADP(H) protects lysine 601 in the porcine NADPH‐cytochrome P‐450 reductase against pyridoxylation

1990 • 5 citations