Flt3 Ligand Enhances the Yield of Primitive Cells After Ex Vivo Cultivation of CD34+ CD38dim Cells and CD34+ CD38dim CD33dim HLA-DR+ Cells

Applied logistic regression

1990 • 37,000 citations

Functional characterization of individual human hematopoietic stem cells cultured at limiting dilution on supportive marrow stromal layers.

1990 • 697 citations

Long-term culture of human bone marrow cells.

1980 • 624 citations

A receptor tyrosine kinase specific to hematopoietic stem and progenitor cell-enriched populations

1991 • 606 citations

Molecular cloning of a ligand for the flt3flk-2 tyrosine kinase receptor: A proliferative factor for primitive hematopoietic cells

1993 • 546 citations

STK-1, the human homolog of Flk-2/Flt-3, is selectively expressed in CD34+ human bone marrow cells and is involved in the proliferation of early progenitor/stem cells.

1994 • 459 citations

Precursors of colony-forming cells in humans can be distinguished from colony-forming cells by expression of the CD33 and CD34 antigens and light scatter properties.

1989 • 453 citations

Ligand for FLT3/FLK2 receptor tyrosine kinase regulates growth of haematopoietic stem cells and is encoded by variant RNAs

1994 • 453 citations

Self-renewal of primitive human hematopoietic cells (long-term-culture-initiating cells) in vitro and their expansion in defined medium.

1996 • 393 citations

Differential cytokine effects on primitive (CD34+CD38-) human hematopoietic cells: novel responses to Flt3-ligand and thrombopoietin.

1996 • 360 citations

Human FLT3/FLK2 gene: cDNA cloning and expression in hematopoietic cells

1993 • 345 citations

Ex vivo expansion of enriched peripheral blood CD34+ progenitor cells by stem cell factor, interleukin-1 beta (IL-1 beta), IL-6, IL-3, interferon-gamma, and erythropoietin

1993 • 334 citations

Cloning of the human homologue of the murine flt3 ligand: a growth factor for early hematopoietic progenitor cells

1994 • 319 citations

Lymphoid and myeloid differentiation of single human CD34+, HLA-DR+, CD38- hematopoietic stem cells

1994 • 314 citations

Murine Flt3, a gene encoding a novel tyrosine kinase receptor of the PDGFR/CSF1R family.

1991 • 300 citations

Extended long-term culture reveals a highly quiescent and primitive human hematopoietic progenitor population

1996 • 281 citations

Large-scale expansion of human stem and progenitor cells from bone marrow mononuclear cells in continuous perfusion cultures

1993 • 280 citations

Endosteal Marrow: A Rich Source of Hematopoietic Stem Cells

1978 • 251 citations

Human FLT3/FLK2 receptor tyrosine kinase is expressed at the surface of normal and malignant hematopoietic cells.

1996 • 250 citations

Direct contact between human primitive hematopoietic progenitors and bone marrow stroma is not required for long-term in vitro hematopoiesis

1992 • 245 citations

Flt3 ligand induces proliferation of quiescent human bone marrow CD34+CD38- cells and maintains progenitor cells in vitro

1996 • 240 citations

Multi-level effects of flt3 ligand on human hematopoiesis: expansion of putative stem cells and proliferation of granulomonocytic progenitors/monocytic precursors

1995 • 240 citations

Long-term erythropoiesis from constant numbers of CD34+ cells in serum-free cultures initiated with highly purified progenitor cells from human bone marrow.

1992 • 231 citations

Murine marrow cells expanded in culture with IL-3, IL-6, IL-11, and SCF acquire an engraftment defect in normal hosts.

1995 • 173 citations

Soluble factor(s) produced by human bone marrow stroma increase cytokine-induced proliferation and maturation of primitive hematopoietic progenitors while preventing their terminal differentiation

1993 • 173 citations

Characterization of primitive hematopoietic cells in normal human peripheral blood

1992 • 172 citations

Flt3 ligand stimulates/costimulates the growth of myeloid stem/progenitor cells.

1995 • 168 citations

Bone marrow transplantation with interleukin-1 plus kit-ligand ex vivo expanded bone marrow accelerates hematopoietic reconstitution in mice without the loss of stem cell lineage and proliferative potential

1993 • 166 citations

The flt3 ligand supports proliferation of lymphohematopoietic progenitors and early B-lymphoid progenitors

1995 • 162 citations

Continuous human bone marrow culture: Ia antigen characterization of probable pluripotential stem cells

1980 • 160 citations

Effect of flt3 ligand on the ex vivo expansion of human CD34+ hematopoietic progenitor cells

1995 • 159 citations

Cellular and molecular characterization of the role of the flk-2/flt-3 receptor tyrosine kinase in hematopoietic stem cells

1994 • 153 citations

The "common stem cell" hypothesis reevaluated: human fetal bone marrow contains separate populations of hematopoietic and stromal progenitors

1995 • 151 citations

Evaluation of ex vivo expansion potential of cord blood and bone marrow hematopoietic progenitor cells using cell tracking and limiting dilution analysis

1995 • 148 citations

Maintenance of transplantation potential in ex vivo expanded CD34(+)- selected human peripheral blood progenitor cells

1994 • 139 citations

Amplification of Sca-1+ Lin-WGA+ cells in serum-free cultures containing steel factor, interleukin-6, and erythropoietin with maintenance of cells with long-term in vivo reconstituting potential

1994 • 139 citations

Long-term generation and expansion of human primitive hematopoietic progenitor cells in vitro

1993 • 135 citations

Expansion of Hematopoietic Progenitor Cell Populations in Stirred Suspension Bioreactors of Normal Human Bone Marrow Cells

1994 • 131 citations

FLT3/FLK2 ligand promotes the growth of murine stem cells and the expansion of colony-forming cells and spleen colony-forming units

1995 • 129 citations

Ex vivo expansion of hematopoietic stem and progenitor cells--robbing Peter to pay Paul? [editorial]

1993 • 129 citations

Expression of CD33, CD38, and HLA-DR on CD34+ human fetal liver progenitors with a high proliferative potential

1994 • 123 citations

The architecture of bone marrow cell populations

1990 • 113 citations

FLK-2/FLT-3 ligand regulates the growth of early myeloid progenitors isolated from human fetal liver

1995 • 112 citations

Long-term culture-initiating cell expansion is dependent on frequent medium exchange combined with stromal and other accessory cell effects

1995 • 105 citations

Cell cycle and functional differences between CD34+/CD38hi and CD34+/38lo human marrow cells after in vitro cytokine exposure

1995 • 100 citations

Phenotypic and functional characterization of long-term culture- initiating cells present in peripheral blood progenitor collections of normal donors treated with granulocyte colony-stimulating factor

1996 • 94 citations

Hematopoietic Transplant Potential of Unrelated Cord Blood: Critical Issues

1996 • 91 citations

Effects of CD34+ cell selection and perfusion on ex vivo expansion of peripheral blood mononuclear cells

1995 • 84 citations

An analysis of the multilineage production of human hematopoietic progenitors in long-term bone marrow culture: evidence that reactive oxygen intermediates derived from mature phagocytic cells have a role in limiting progenitor cell self-renewal

1988 • 67 citations

A novel single-cell proliferation assay shows that long-term culture- initiating cell (LTC-IC) maintenance over time results from the extensive proliferation of a small fraction of LTC-IC

1995 • 61 citations

Long-term reconstitution of mice after ex vivo expansion of bone marrow cells: differential activity of cultured bone marrow and enriched stem cell populations.

1994 • 58 citations

Biology and potential clinical applications of flt3 ligand

1995 • 33 citations

Early B-lymphocyte precursor cells in mouse bone marrow: subosteal localization of B220+ cells during postirradiation regeneration.

1990 • 29 citations

Detection and quantitation of long-term culture-initiating cells in normal human peripheral blood.

1992 • 26 citations

Accelerated and long-term hematopoietic engraftment in mice transplanted with ex vivo expanded bone marrow.

1994 • 25 citations

Removal of peripheral blood monocytes by phenylalanine methyl ester has no effect on the colony growth of hematopoietic progenitor cells

1989 • 22 citations

Basic fibroblast growth factor and epidermal growth factor downmodulate the growth of hematopoietic cells in long‐term stromal cultures

1995 • 20 citations

A new density gradient for the separation of large quantities of rosette-positive and rosette-negative cells.

1987 • 14 citations

Purification of peripheral blood hematopoietic cells using Campath-1M monoclonal antibody.

1990 • 6 citations

Ex-vivo Expansion of Normal Human Bone-marrow Cd34+dr(low) Cells in Liquid Culture

1995 • 1 citations