Evidence for a Large Compartment of IgM-Expressing Memory B Cells in Humans

Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction

1987 • 64,270 citations

Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction

1987 • 45,673 citations

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Rapid flow cytofluorometric analysis of mammalian cell cycle by propidium iodide staining.

1975 • 1,734 citations

Intraclonal generation of antibody mutants in germinal centres

1991 • 1,083 citations

Maturation of the immune response in germinal centers

1991 • 904 citations

Tracing B cell development in human germinal centres by molecular analysis of single cells picked from histological sections.

1993 • 691 citations

Analysis of somatic mutation in five B cell subsets of human tonsil.

1994 • 667 citations

Generation of Memory B Cells and Plasma Cells in Vitro

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B4, a human B lymphocyte-associated antigen expressed on normal, mitogen-activated, and malignant B lymphocytes.

1983 • 517 citations

Germinal centres in T-cell-dependent antibody responses

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1993 • 413 citations

Cells in the Marginal Zone of the Spleen

1992 • 411 citations

Elements regulating somatic hypermutation of an immunoglobulin κ gene: Critical role for the intron enhancer/matrix attachment region

1994 • 382 citations

Antigens in Immunity

1966 • 372 citations

Memory B cells from human tonsils colonize mucosal epithelium and directly present antigen to T cells by Rapid Up-Regulation of B7-1 and B7-2

1995 • 358 citations

Maintenance of B-cell memory by long-lived cells generated from proliferating precursors

1990 • 323 citations

Hypermutation generating the sheep immunoglobulin repertoire is an antigen-independent process

1995 • 318 citations

Evolution of human immunoglobulin kappa J region genes.

1982 • 318 citations

Analysis of mutations in immunoglobulin heavy chain variable region genes of microdissected marginal zone (MGZ) B cells suggests that the MGZ of human spleen is a reservoir of memory B cells.

1995 • 292 citations

Memory B cells in T cell-dependent antibody responses colonize the splenic marginal zones

1988 • 272 citations

Somatic generation of diversity in a mammalian primary lymphoid organ: The sheep ileal Peyer's patches

1991 • 269 citations

Interleukin 2 receptors on human B cells. Implications for the role of interleukin 2 in human B cell function.

1985 • 249 citations

Somatic hypermutation

1995 • 248 citations

Fc epsilon receptor, a specific differentiation marker transiently expressed on mature B cells before isotype switching.

1986 • 240 citations

A directory of human germ‐line V_χ segments reveals a strong bias in their usage

1994 • 220 citations

The maturation of the immune response

1993 • 219 citations

Maturation and Dispersal of B‐Cell Clones during T Cell‐Dependent Antibody Responses

1992 • 206 citations

The repertoire of somatic antibody mutants accumulating in the memory compartment after primary immunization is restricted through affinity maturation and mirrors that expressed in the secondary response.

1990 • 200 citations

Immunoglobulin switch transcript production in vivo related to the site and time of antigen-specific B cell activation.

1996 • 199 citations

Somatic hypermutation in low-grade mucosa-associated lymphoid tissue- type B-cell lymphoma

1995 • 182 citations

The human gut contains a novel population of B lymphocytes which resemble marginal zone cells.

1985 • 168 citations

Human IgM⁺IgD⁺ B cells, the major B cell subset in the peripheral blood, express Vϰ genes with no or little somatic mutation throughout life

1993 • 166 citations

Cell cycle regulation of the glyceraldehyde3phosphate dehydrogenaseluracil DNA glycosylase gene in normal human cells

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B cell development in the bone marrow.

1990 • 148 citations

Immunoglobulin heavy chain gene expression in peripheral blood B lymphocytes.

1992 • 142 citations

Germinal Centers and Antibody Production in Bone Marrow

1992 • 135 citations

Phenotypic and Functional Characterization of Human LEUI (CD5) B Cells

1986 • 130 citations

Expressed human immunoglobulin ϰ genes and their hypermutation

1993 • 130 citations

Preferential use of the VH4 Ig gene family by diffuse large-cell lymphoma

1995 • 129 citations

Isolation of high-affinity memory B cells: phycoerythrin as a probe for antigen-binding cells.

1987 • 126 citations

Heterogeneity of M-cell-associated B and T cells in human Peyer's patches.

1994 • 121 citations

The variable genes of the human immunoglobulin kappa locus.

1993 • 120 citations

Transforming growth factor type β regulation of actin mRNA

1986 • 116 citations

Detection of clonal B cell populations in paraffin-embedded tissues by polymerase chain reaction.

1993 • 116 citations

Variable region gene analysis of B cell subsets derived from a 4-year-old child: somatically mutated memory B cells accumulate in the peripheral blood already at young age.

1994 • 108 citations

Antigens in immunity. VII. Analysis of immunological memory.

1965 • 107 citations

Burkitt's lymphomas express VH genes with a moderate number of antigen-selected somatic mutations.

1995 • 103 citations

Transcriptional and posttranscriptional control of immunoglobulin mRNA production during Blymphocyte development

1986 • 99 citations

Burkitt’s Lymphoma Is a Malignancy of Mature B Cells Expressing Somatically Mutated V Region Genes

1995 • 98 citations

Germline variable region gene segment derivation of human monoclonal anti-Rh(D) antibodies. Evidence for affinity maturation by somatic hypermutation and repertoire shift.

1992 • 93 citations

Human immunoglobulin VH and VK repertoire revealed by in situ hybridization

1990 • 89 citations

Splenic marginal zone lymphocytes and related cells in the lymph node: A morphologic and immunohistochemical study

1989 • 88 citations

A majority of Ig H chain cDNA of normal human adult blood lymphocytes resembles cDNA for fetal Ig and natural autoantibodies.

1993 • 81 citations

High frequency of somatically mutated IgM molecules in the human adult blood B cell repertoire

1992 • 80 citations

The Marginal Zone in the Human Reactive Lymph Node

1986 • 76 citations

Human peripheral blood B lymphocyte subpopulations: functional and phenotypic analysis of surface IgD positive and negative subsets.

1986 • 73 citations

Natural occurrence and origin of somatically mutated memory B cells in mice.

1992 • 71 citations

Somatic mutation and CDR3 lengths of immunoglobulin kappa light chains expressed in patients with rheumatoid arthritis and in normal individuals.

1995 • 71 citations

Immature sinus histiocytosis. Light- and electron-microscopic features, immunologic phenotype, and relationship with marginal zone lymphocytes.

1985 • 66 citations

Sequence analysis of rearranged IgVH genes from microdissected human Peyer's patch marginal zone B cells.

1996 • 65 citations

Molecular Ig gene analysis reveals that monocytoid B cell lymphoma is a malignancy of mature B cells carrying somatically mutated V region genes and suggests that rearrangement of the kappa‐deleting element (resulting in deletion of the Ig kappa enhancers) abolishes somatic hypermutation in the human

1996 • 62 citations

DETERMINATION OF ANTIBODY CLASS IN A SYSTEM OF COOPERATING ANTIGENIC DETERMINANTS

1970 • 48 citations

Heavy-chain class switch does not terminate somatic mutation.

1990 • 43 citations

Traffic of T and B Lymphocytes in the Normal Spleen

1978 • 41 citations

Evidence that the V kappa III gene usage is nonstochastic in both adult and newborn peripheral B cells and that peripheral CD5+ adult B cells are oligoclonal.

1994 • 34 citations

Peripheral human CD5+ and CD5- B cells may express somatically mutated VH5- and VH6-encoded IgM receptors.

1993 • 34 citations

Ongoing V_x−J_x recombination after formation of a productiveV_x−J_x coding joint

1992 • 30 citations

Switch recombination in normal IgA1+ Blymphocytes.

1994 • 26 citations

Isolation of germinal centerlike events from human spleen RNA. Somatic hypermutation of a clonally related VH6DJH rearrangement expressed with IgM, IgG, and IgA.

1993 • 23 citations

Isolation and Characterization of Human Antigen-Specific B Lymphocytes

1994 • 22 citations

A phenotypic study of B lymphocyte subpopulations in human bone marrow

1990 • 11 citations

[The spleen, an unnecessary organ?].

1981 • 8 citations