FKBP12-rapamycin target TOR2 is a vacuolar protein with an associated phosphatidylinositol-4 kinase activity.

Antibodies, a laboratory manual

1989 • 12,929 citations

Site-directed mutagenesis by overlap extension using the polymerase chain reaction

1989 • 7,496 citations

Inositol trisphosphate and calcium signalling

1993 • 6,546 citations

Inositol phosphates and cell signalling

1989 • 4,058 citations

Calcineurin is a common target of cyclophilin-cyclosporin A and FKBP-FK506 complexes

1991 • 4,043 citations

New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites

1988 • 2,903 citations

The mechanism of action of cyclosporin A and FK506

1992 • 2,127 citations

A mammalian protein targeted by G1-arresting rapamycin–receptor complex

1994 • 1,954 citations

Targets for Cell Cycle Arrest by the Immunosuppressant Rapamycin in Yeast

1991 • 1,937 citations

Identification of calcineurin as a key signalling enzyme in T-lymphocyte activation

1992 • 1,634 citations

Chemistry and Biology of the Immunophilins and Their Immunosuppressive Ligands

1991 • 1,479 citations

RAFT1: A mammalian protein that binds to FKBP12 in a rapamycin-dependent fashion and is homologous to yeast TORs

1994 • 1,470 citations

Biological Membranes as Bilayer Couples. A Molecular Mechanism of Drug-Erythrocyte Interactions

1974 • 1,362 citations

Rapamycin-FKBP specifically blocks growth-dependent activation of and signaling by the 70 kd S6 protein kinases

1992 • 1,146 citations

Phosphatidylinositol 3-Kinase Encoded by Yeast VPS 34 Gene Essential for Protein Sorting

1993 • 909 citations

FK-506- and CsA-sensitive activation of the interleukin-2 promoter by calcineurin

1992 • 865 citations

Isolation of a Protein Target of the FKBP12-Rapamycin Complex in Mammalian Cells

1995 • 859 citations

Morphological classification of the yeast vacuolar protein sorting mutants: evidence for a prevacuolar compartment in class E vps mutants.

1992 • 832 citations

Target of rapamycin in yeast, TOR2, is an essential phosphatidylinositol kinase homolog required for G1 progression

1993 • 832 citations

The fungal vacuole: composition, function, and biogenesis

1990 • 758 citations

Two distinct signal transmission pathways in T lymphocytes are inhibited by complexes formed between an immunophilin and either FK506 or rapamycin.

1990 • 699 citations

Phosphatidylinositol 3-kinase: Structure and expression of the 110 kd catalytic subunit

1992 • 692 citations

Rapamycin-Induced Inhibition of the 70-Kilodalton S6 Protein Kinase

1992 • 666 citations

Phosphatidylinositol 3‐kinase

1994 • 649 citations

Modulation of gelsolin function by phosphatidylinositol 4,5-bisphosphate

1987 • 624 citations

Distinct mechanisms of suppression of murine T cell activation by the related macrolides FK-506 and rapamycin.

1990 • 613 citations

[20] In vitro mutagenesis and plasmid shuffling: From cloned gene to mutant yeast

1991 • 584 citations

Inhibition of T cell signaling by immunophilin-ligand complexes correlates with loss of calcineurin phosphatase activity

1992 • 561 citations

An essential role for a phospholipid transfer protein in yeast Golgi function

1990 • 534 citations

Chapter 19 Fluorescence Microscopy Methods for Yeast

1989 • 528 citations

Phosphoinositides and Calcium as Regulators of Cellular Actin Assembly and Disassembly

1994 • 524 citations

ATP-dependent inositide phosphorylation required for Ca2+-activated secretion

1995 • 508 citations

RAPT1, a mammalian homolog of yeast Tor, interacts with the FKBP12/rapamycin complex.

1994 • 475 citations

The Actin-Binding Protein Profilin Binds to PIP ₂ and Inhibits Its Hydrolysis by Phospholipase C

1990 • 472 citations

Structure and function of proton translocating ATPase in plasma membranes of plants and fungi

1988 • 464 citations

FK506 and cyclosporin, molecular probes for studying intracellular signal transduction

1993 • 389 citations

TOR1 and TOR2 are structurally and functionally similar but not identical phosphatidylinositol kinase homologues in yeast.

1994 • 378 citations

The immunosuppressive macrolides FK-506 and rapamycin act as reciprocal antagonists in murine T cells.

1990 • 360 citations

The actions of cyclosporin A and FK506 suggest a novel step in the activation of T lymphocytes.

1990 • 353 citations

[33] High-expression vectors with multiple cloning sites for construction of trpE fusion genes: pATH vectors

1991 • 350 citations

[44] Methods for studying the yeast vacuole

1991 • 346 citations

A membrane-associated complex containing the Vps15 protein kinase and the Vps34 PI 3-kinase is essential for protein sorting to the yeast lysosome-like vacuole.

1993 • 340 citations

The fungal vacuole: composition, function, and biogenesis.

1990 • 308 citations

Yeast has homologs (CNA1 and CNA2 gene products) of mammalian calcineurin, a calmodulin-regulated phosphoprotein phosphatase.

1991 • 297 citations

FK 506-binding protein proline rotamase is a target for the immunosuppressive agent FK 506 in Saccharomyces cerevisiae.

1991 • 292 citations

Suppressors of yeast actin mutations.

1989 • 286 citations

Protein phosphatase type 2B (calcineurin)-mediated, FK506-sensitive regulation of intracellular ions in yeast is an important determinant for adaptation to high salt stress conditions.

1993 • 274 citations

Dominant Missense Mutations in a Novel Yeast Protein related to Mammalian Phosphatidylinositol 3-Kinase and VPS34 Abrogate Rapamycin Cytotoxicity

1993 • 271 citations

Recent insights in phosphatidylinositol signaling

1990 • 245 citations

Positive regulation of the cAMP-responsive activator CREM by the p70 S6 kinase: An alternative route to mitogen-induced gene expression

1994 • 233 citations

An essential gene, ESR1, is required for mitotic growth, DNA repair and meiotic recombination Saccharomyces cerevisiae

1994 • 215 citations

Rapamycin Sensitivity in Saccharomyces cerevisiae Is Mediated by a Peptidyl-Prolyl cis-trans Isomerase Related to Human FK506-Binding Protein

1991 • 213 citations

Phosphatidylinositol 4-Kinase: Gene Structure and Requirement for Yeast Cell Viability

1993 • 211 citations

Dominant missense mutations in a novel yeast protein related to mammalian phosphatidylinositol 3-kinase and VPS34 abrogate rapamycin cytotoxicity.

1993 • 202 citations

Calcineurin mediates inhibition by FK506 and cyclosporin of recovery from α-factor arrest in yeast

1992 • 195 citations

A novel gene, STT4, encodes a phosphatidylinositol 4-kinase in the PKC1 protein kinase pathway of Saccharomyces cerevisiae.

1994 • 191 citations

TheSaccharomyces cerevisiae genes (CMP1 andCMP2) encoding calmodulin-binding proteins homologous to the catalytic subunit of mammalian protein phosphatase 2B

1991 • 179 citations

Immunophilins interact with calcineurin in the absence of exogenous immunosuppressive ligands.

1994 • 162 citations

Primary structure and disruption of the phosphatidylinositol synthase gene of Saccharomyces cerevisiae.

1987 • 151 citations

Identification and quantification of polyphosphoinositides produced in response to platelet-derived growth factor stimulation

1991 • 149 citations

FKBP-rapamycin inhibits a cyclin-dependent kinase activity and a cyclin D1-Cdk association in early G1 of an osteosarcoma cell line.

1993 • 149 citations

The Sequence of Phosphatidylinositol-4-phosphate 5-Kinase Defines a Novel Family of Lipid Kinases

1995 • 141 citations

Calcineurin is essential in cyclosporin A- and FK506-sensitive yeast strains.

1994 • 140 citations

Rapamycin selectively inhibits the growth of childhood rhabdomyosarcoma cells through inhibition of signaling via the type I insulin-like growth factor receptor.

1994 • 134 citations

A protein kinase antigenically related to pp60v-src possibly involved in yeast cell cycle control: positive in vivo regulation by sterol.

1987 • 124 citations

Formation of phosphatidylinositol 3-phosphate by isomerization from phosphatidylinositol 4-phosphate.

1991 • 123 citations

Essential role for phosphatidylinositol 4,5-bisphosphate in yeast cell proliferation

1988 • 121 citations

The gene encoding the phosphatidylinositol transfer protein is essential for cell growth.

1990 • 118 citations

PIK1, an essential phosphatidylinositol 4-kinase associated with the yeast nucleus.

1994 • 117 citations

Targets of immunophilin-immunosuppressant complexes are distinct highly conserved regions of calcineurin A.

1995 • 115 citations

In vitro reactions of vacuole inheritance in Saccharomyces cerevisiae.

1992 • 113 citations

Molecular analysis of the yeast VPS3 gene and the role of its product in vacuolar protein sorting and vacuolar segregation during the cell cycle.

1990 • 112 citations

Rapamycin-FKBP12 blocks proliferation, induces differentiation, and inhibits cdc2 kinase activity in a myogenic cell line.

1993 • 105 citations

Changes in phospholipids of Saccharomyces cerevisiae associated with inositol-less death

1977 • 104 citations

Activation of a low specific activity form of DNA polymerase α by inositol-1,4-bisphosphate

1988 • 103 citations

Intervacuole Exchange in the Yeast Zygote: A New Pathway in Organelle Communication

1988 • 100 citations

Cloning and characterization of a human phosphatidylinositol 4-kinase.

1994 • 95 citations

Proline isomerases at the crossroads of protein folding, signal transduction, and immunosuppression.

1992 • 93 citations

Analysis of inositol metabolites produced by Saccharomyces cerevisiae in response to glucose stimulation.

1993 • 72 citations

Characterization of the END1 gene required for vacuole biogenesis and gluconeogenic growth of budding yeast.

1989 • 72 citations

The composition and morphology of yeast nuclear scaffolds

1990 • 57 citations

Stimulation of cell proliferation and polyphosphoinositide metabolism in Saccharomycescerevisiae GL7 by ergosterol

1985 • 54 citations

Protein regulation by phosphatidylinositol lipids

1995 • 53 citations

CDC25‐dependent induction of inositol 1,4,5‐trisphosphate and diacylglycerol in Saccharomyces cerevisiae by nitrogen

1992 • 41 citations

Inositol polyphosphate 1-phosphatase is present in the nucleus and inhibits DNA synthesis.

1994 • 34 citations

Identification of Immunosuppressive Drug Targets in Yeast

1993 • 34 citations

Photoaffinity labeling of the 45-kDa and 55-kDa forms of phosphatidylinositol 4-kinase from the yeast Saccharomyces cerevisiae.

1993 • 3 citations