DNA methylation and cancer

Mutation and Cancer: Statistical Study of Retinoblastoma

1971 • 7,335 citations

Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands.

1996 • 5,667 citations

CpG-rich islands and the function of DNA methylation

1986 • 3,760 citations

Transcriptional repression by the methyl-CpG-binding protein MeCP2 involves a histone deacetylase complex

1998 • 3,363 citations

A genomic sequencing protocol that yields a positive display of 5-methylcytosine residues in individual DNA strands.

1992 • 3,099 citations

Methylated DNA and MeCP2 recruit histone deacetylase to repress transcription

1998 • 2,686 citations

Cancer-epigenetics comes of age

1999 • 2,379 citations

5′ CpG island methylation is associated with transcriptional silencing of the tumour suppressor p16/CDKN2/MTS1 in human cancers

1995 • 2,000 citations

Alterations in DNA Methylation: A Fundamental Aspect of Neoplasia

1997 • 1,960 citations

Synergy of demethylation and histone deacetylase inhibition in the re-expression of genes silenced in cancer

1999 • 1,865 citations

High sensitivity mapping of methylated cytosines

1994 • 1,863 citations

Incidence and functional consequences of hMLH1 promoter hypermethylation in colorectal carcinoma

1998 • 1,852 citations

Cellular differentiation, cytidine analogs and DNA methylation

1980 • 1,803 citations

Silencing of the VHL tumor-suppressor gene by DNA methylation in renal carcinoma.

1994 • 1,542 citations

MISMATCH REPAIR IN REPLICATION FIDELITY, GENETIC RECOMBINATION, AND CANCER BIOLOGY

1996 • 1,485 citations

Methylation of the hMLH1 promoter correlates with lack of expression of hMLH1 in sporadic colon tumors and mismatch repair-defective human tumor cell lines.

1997 • 1,465 citations

Inactivation of the CDKN2/p16/MTS1 gene is frequently associated with aberrant DNA methylation in all common human cancers.

1995 • 1,353 citations

Purification, sequence, and cellular localization of a novel chromosomal protein that binds to Methylated DNA

1992 • 1,331 citations

MeCP2 Is a Transcriptional Repressor with Abundant Binding Sites in Genomic Chromatin

1997 • 1,258 citations

Methylation of the oestrogen receptor CpG island links ageing and neoplasia in human colon

1994 • 1,205 citations

COBRA: a sensitive and quantitative DNA methylation assay

1997 • 1,184 citations

Amount and distribution of 5-methylcytosine in human DNA from different types of tissues or cells

1982 • 1,002 citations

Detection of aberrant promoter hypermethylation of tumor suppressor genes in serum DNA from non-small cell lung cancer patients.

1999 • 920 citations

Cloning and sequencing of a cDNA encoding DNA methyltransferase of mouse cells

1988 • 891 citations

The 5-methylcytosine content of DNA from human tumors

1983 • 835 citations

The human DNA methyltransferases (DNMTs) 1, 3a and 3b: coordinate mRNA expression in normal tissues and overexpression in tumors

1999 • 830 citations

E-cadherin expression is silenced by DNA hypermethylation in human breast and prostate carcinomas.

1995 • 803 citations

Effects of DNA methylation on DNA-binding proteins and gene expression

1993 • 732 citations

Developmental pattern of gene-specific DNA methylation in the mouse embryo and germ line.

1992 • 729 citations

DNA methylation inhibits transcription indirectly via a methyl-CpG binding protein

1991 • 722 citations

Suppression of intestinal neoplasia by DNA hypomethylation

1995 • 712 citations

Toxicity of 5-aza-2'-deoxycytidine to mammalian cells is mediated primarily by covalent trapping of DNA methyltransferase rather than DNA demethylation.

1994 • 667 citations

Identification of a mammalian protein that binds specifically to DNA containing methylated CpGs

1989 • 655 citations

5-Methylcytosine as an Endogenous Mutagen in the Human LDL Receptor and p53 Genes

1990 • 643 citations

Silencing of the E-cadherin invasion-suppressor gene by CpG methylation in human carcinomas.

1995 • 635 citations

Biallelic inactivation of hMLH 1 by epigenetic gene silencing, a novel mechanism causing human MSI cancers

1998 • 629 citations

A mammalian protein with specific demethylase activity for mCpG DNA

1999 • 624 citations

Hypermethylation-associated inactivation indicates a tumor suppressor role for p15INK4B.

1996 • 585 citations

Loss of Heterozygosity in Normal Tissue Adjacent to Breast Carcinomas

1996 • 583 citations

CpG methylation of the cAMP-responsive enhancer/promoter sequence TGACGTCA abolishes specific factor binding as well as transcriptional activation.

1989 • 554 citations

How does DNA methylation repress transcription?

1997 • 532 citations

Covalent bond formation between a DNA-cytosine methyltransferase and DNA containing 5-azacytosine.

1984 • 500 citations

Reduced genomic 5-methylcytosine content in human colonic neoplasia.

1988 • 491 citations

Methylation of the estrogen receptor gene CpG island marks loss of estrogen receptor expression in human breast cancer cells.

1994 • 481 citations

Identification of differentially methylated sequences in colorectal cancer by methylated CpG island amplification.

1999 • 475 citations

The rate of hydrolytic deamination of 5-methylcytosine in double-stranded DNA

1994 • 448 citations

Cloning, expression and chromosome locations of the human DNMT3 gene family

1999 • 412 citations

Dnmt2 is not required for de novo and maintenance methylation of viral DNA in embryonic stem cells

1998 • 408 citations

CpG island hypermethylation in human colorectal tumors is not associated with DNA methyltransferase overexpression.

1999 • 392 citations

Methylation of the BRCA1 gene in sporadic breast cancer.

1997 • 384 citations

Methylation of CpG in a small region of the hMLH1 promoter invariably correlates with the absence of gene expression.

1999 • 369 citations

Rapid quantitation of methylation differences at specific sites using methylation-sensitive single nucleotide primer extension (Ms-SNuPE)

1997 • 369 citations

Methylation-associated silencing of the tissue inhibitor of metalloproteinase-3 gene suggest a suppressor role in kidney, brain, and other human cancers.

1999 • 366 citations

Inactivation of glutathione S-transferase P1 gene by promoter hypermethylation in human neoplasia.

1998 • 366 citations

Genomic Imprinting: Implications for Human Disease

1999 • 363 citations

The Human ARF Cell Cycle Regulatory Gene Promoter Is a CpG Island Which Can Be Silenced by DNA Methylation and Down-Regulated by Wild-Type p53

1998 • 357 citations

Reaction of sodium bisulfite with uracil, cytosine, and their derivatives

1970 • 344 citations

Cytosine methylation determines hot spots of DNA damage in the human P 53 gene

1997 • 335 citations

X-chromosome inactivation and cell memory

1992 • 329 citations

CpG methylation inactivates the promoter activity of the human retinoblastoma tumor-suppressor gene.

1993 • 317 citations

Increased Cytosine DNA-Methyltransferase Activity During Colon Cancer Progression

1993 • 315 citations

Demethylation of the estrogen receptor gene in estrogen receptor-negative breast cancer cells can reactivate estrogen receptor gene expression.

1995 • 301 citations

Histone Deacetylase: A Regulator of Transcription

1996 • 298 citations

Association between CpG island methylation and microsatellite instability in colorectal cancer.

1997 • 295 citations

Expression of the MAGE-1 tumor antigen is up-regulated by the demethylating agent 5-aza-2'-deoxycytidine.

1994 • 292 citations

Transcriptional silencing of the p73 gene in acute lymphoblastic leukemia and Burkitt's lymphoma is associated with 5' CpG island methylation.

1999 • 285 citations

Tumor-suppressive effect of the retinoic acid receptor beta in human epidermoid lung cancer cells.

1993 • 282 citations

A candidate mammalian DNA methyltransferase related to pmt1p of fission yeast

1998 • 274 citations

Estrogen Receptor Gene Analysis in Estrogen Receptor-Positive and Receptor-Negative Primary Breast Cancer

1995 • 271 citations

CpNpG methylation in mammalian cells

1995 • 259 citations

Methylation of the androgen receptor promoter CpG island is associated with loss of androgen receptor expression in prostate cancer cells.

1998 • 234 citations

Detailed methylation analysis of the glutathione S-transferase π (GSTP1) gene in prostate cancer

1999 • 225 citations

CDKN2 gene silencing in lung cancer by DNA hypermethylation and kinetics of p16INK4 protein induction by 5-aza 2'deoxycytidine.

1995 • 215 citations

Quantitative analysis of aberrant p16 methylation using real-time quantitative methylation-specific polymerase chain reaction.

1999 • 214 citations

Methylation hot spots in the 5' flanking region denote silencing of the O6-methylguanine-DNA methyltransferase gene.

1997 • 214 citations

Mapping of ER gene CpG island methylation-specific polymerase chain reaction.

1998 • 209 citations

Pilot phase l-ll study on 5-aza-2??-deoxycytidine (Decitabine) in patients with metastatic lung cancer

1997 • 209 citations

Mutagenicity of 5-aza-2′-deoxycytidine is mediated by the mammalian DNA methyltransferase

1997 • 207 citations

Identification and characterization of differentially methylated regions of genomic DNA by methylation-sensitive arbitrarily primed PCR.

1997 • 197 citations

CpG methylation within the 5′ regulatory region of the BRCA1 gene is tumor specific and includes a putative CREB binding site

1998 • 192 citations

Establishment of methylation-sensitive–representational difference analysis and isolation of hypo- and hypermethylated genomic fragments in mouse liver tumors

1997 • 186 citations

Down-regulation of retinoic acid receptor beta in mammary carcinoma cell lines and its up-regulation in senescing normal mammary epithelial cells.

1994 • 183 citations

Single nucleotide primer extension to detect genetic diseases: experimental application to hemophilia B (factor IX) and cystic fibrosis genes.

1991 • 174 citations

5-Aza-2'-deoxycytidine induces terminal differentiation of leukemic blasts from patients with acute myeloid leukemias

1984 • 173 citations

Methylation and silencing of the Thrombospondin-1 promoter in human cancer

1999 • 172 citations

Inhibition of tumorigenesis by a cytosine–DNA, methyltransferase, antisense oligodeoxynucleotide

1997 • 171 citations

Loss of retinoic acid receptor beta expression in breast cancer and morphologically normal adjacent tissue but not in the normal breast tissue distant from the cancer.

1997 • 161 citations

Expression of Antisense to DNA Methyltransferase mRNA Induces DNA Demethylation and Inhibits Tumorigenesis

1995 • 155 citations

Mechanisms of inactivation of E-cadherin in breast cancer cell lines.

1998 • 148 citations

The tumorigenicity of 5-azacytidine in the male Fischer rat

1984 • 143 citations

Clinical trial on 5-AZA-2′-deoxycytidine in patients with acute leukemia

1985 • 141 citations

Results of decitabine therapy in the accelerated and blastic phases of chronic myelogenous leukemia

1997 • 140 citations

A Phase II Study of 5-AZA-2'Deoxycytidine (Decitabine) in Hormone Independent Metastatic (D2) Prostate Cancer

1998 • 139 citations

Frequent loss of chromosome 9 in human primary non-small cell lung cancer.

1994 • 136 citations

Low frequency of p16/CDKN2A methylation in sporadic melanoma: comparative approaches for methylation analysis of primary tumors.

1997 • 129 citations

Prolonged Upregulation of the Expression of HLA Class I Antigens and Co stimulatory Molecules on Melanoma Cells Treated with 5-aza-2??-deoxycytidine (5-AZA-CdR)

1999 • 126 citations

Identification of DNA methylation markers for human breast carcinomas using the methylation-sensitive restriction fingerprinting technique.

1997 • 123 citations

Inhibition of DNA methylation in L1210 leukemic cells by 5-aza-2'-deoxycytidine as a possible mechanism of chemotherapeutic action.

1983 • 118 citations

Phase I and pharmacokinetic study of 5-aza-2'-deoxycytidine (NSC 127716) in cancer patients.

1986 • 118 citations

Formation of methylation patterns in the mammalian genome

1997 • 114 citations

Gene methylation patterns and expression

1993 • 114 citations

Review

1998 • 110 citations

Incorporation of 5-Aza-2'-deoxycytidine-5'-triphosphate into DNA. Interactions with mammalian DNA polymerase alpha and DNA methylase.

1983 • 102 citations

Demethylation by 5-aza-2??-deoxycytidine of specific 5-methylcytosine sites in the promoter region of the retinoic acid receptor ?? gene in human colon carcinoma cells

1998 • 99 citations

Mutagenicity of 5-azacytidine and related nucleosides in C3H/10T 1/2 clone 8 and V79 cells.

1982 • 98 citations

A protein from human placental nuclei binds preferentially to 5-methylcytosine-rich DNA

1984 • 98 citations

Drug resistance to 5-aza-2 ′ -deoxycytidine, 2 ′ ,2 ′ -difluorodeoxycytidine, and cytosine arabinoside conferred by retroviral-mediated transfer of human cytidine deaminase cDNA into murine cells

1998 • 92 citations

The heterogeneity of thymine methyl group origin in DNA pyrimidine isostichs of developing sea urchin embryos.

1967 • 89 citations

Molecular, cellular and animal pharmacology of 5-AZA-2′-deoxycytidine

1985 • 89 citations

5-Aza-2'-deoxycytidine (Decitabine) induces trilineage response in unfavourable myelodysplastic syndromes.

1993 • 89 citations

Rapid Analysis of DNA Methylation Using New Restriction Enzyme Sites Created by Bisulfite Modification

1996 • 88 citations

Comparison of the antileukemic activity of 5-aza-2′-deoxycytidine, 1-β-d-arabinofuranosylcytosine and 5-azacytidine against L1210 leukemia

1984 • 84 citations

The DNA methylation machinery as a target for anticancer therapy

1996 • 84 citations

Hypomethylation of DNA: An epigenetic mechanism involved in tumor promotion

1994 • 81 citations

Host-Cell-Determined Methylation of Specific Epstein-Barr Virus Promoters Regulates the Choice between Distinct Viral Latency Programs

1997 • 81 citations

Multiple functions of dynamic histone acetylation

1994 • 79 citations

The repressor MDBP-2 is a member of the histone H1 family that binds preferentially in vitro and in vivo to methylated nonspecific DNA sequences.

1992 • 79 citations

DNA methylation of retinoic acid receptor β in breast cancer and possible therapeutic role of 5-aza-2ʼ-deoxycytidine

1999 • 74 citations

Activation of the retinoic acid receptor ?? gene by 5-aza-2???-deoxycytidine in human DLD-1 colon carcinoma cells

1997 • 68 citations

Induction of differentiation and inhibition of DNA methylation in HL-60 myeloid leukemic cells by 5-AZA-2′-deoxycytidine

1985 • 67 citations

Restriction landmark genomic scanning (RLGS-M)-based genome-wide scanning of mouse liver tumors for alterations in DNA methylation status.

1997 • 62 citations

5-AZA-2′-deoxycytidine therapy in patients with acute leukemia inhibits DNA methylation

1984 • 61 citations

Down-regulation of CD44 expression in human prostatic carcinoma cell lines is correlated with DNA hypermethylation

1999 • 55 citations

Carcinogenicity and haemoglobin synthesis induction by cytidine analogues

1988 • 53 citations

Silencing of the mammary-derived growth inhibitor (MDGI) gene in breast neoplasms is associated with epigenetic changes.

1996 • 53 citations

Genes and genomes: Sequencing 5‐methylcytosine residues in genomic DNA

1994 • 49 citations

Response of four human ovarian carcinoma cell lines to ALL‐TRANS retinoic acid: Relationship with induction of differentiation and retinoic acid receptor expression

1994 • 49 citations

Cell cycle effects and cellular pharmacology of 5-AZA-2?-deoxycytidine

1984 • 47 citations

Cytosines Adjacent to Methylated CpG Sites Can Be Partially Resistant to Conversion in Genomic Bisulfite Sequencing Leading to Methylation Artifacts

1998 • 45 citations

G1 Cyclins and Control of the Cell Division Cycle in Normal and Transformed Cells

1997 • 42 citations

Transcriptional regulation of the mts1 gene in human lymphoma cells: the role of DNA-methylation

1995 • 42 citations

Use of a tissue-specific promoter for targeted expression of the herpes simplex virus thymidine kinase gene in cervical carcinoma cells.

1998 • 33 citations

A region within murine chromosome 7F4, syntenic to the human 11q13 amplicon, is frequently amplified in 4NQO-induced oral cavity tumors>

1997 • 28 citations

Retinoic acid receptor beta induces terminal differentiation of squamous cell carcinoma lines in the absence of cyclin-dependent kinase inhibitor expression.

1998 • 24 citations

Effect of trna from 5-azacytidine-treated hamster fibrosarcoma cells on protein synthesis in vitro in a cell-free system

1976 • 23 citations

Preclinical antitumor activity of 5-aza-2′-deoxycytidine agains human head and neck cancer xenografts

1988 • 15 citations