Vma21p is a yeast membrane protein retained in the endoplasmic reticulum by a di-lysine motif and is required for the assembly of the vacuolar H(+)-ATPase complex.

Basic local alignment search tool

1990 • 87,358 citations

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

Basic Local Alignment Search Tool

1990 • 12,947 citations

A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.

1989 • 8,705 citations

[19] Rapid and efficient site-specific mutagenesis without phenotypic selection

1987 • 5,413 citations

Methods in Yeast Genetics

1988 • 4,905 citations

A ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for transformaion of Escherichia coli

1987 • 2,493 citations

A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance

1984 • 2,401 citations

ACIDIFICATION OF THE ENDOCYTIC AND EXOCYTIC PATHWAYS

1986 • 1,977 citations

Isolation of intracellular membranes by means of sodium carbonate treatment: application to endoplasmic reticulum.

1982 • 1,765 citations

A Saccharomyces cerevisiae genomic plasmid bank based on a centromere-containing shuttle vector

1987 • 1,181 citations

Identification of a consensus motif for retention of transmembrane proteins in the endoplasmic reticulum.

1990 • 918 citations

Expression of wild-type and mutant forms of influenza hemagglutinin: The role of folding in intracellular transport

1986 • 879 citations

The structure of an antigenic determinant in a protein

1984 • 841 citations

Morphological classification of the yeast vacuolar protein sorting mutants: evidence for a prevacuolar compartment in class E vps mutants.

1992 • 832 citations

Yeast plasma membrane ATPase is essential for growth and has homology with (Na+ + K+), K+- and Ca2+-ATPases

1986 • 801 citations

KAR2, a karyogamy gene, is the yeast homolog of the mammalian BiP/GRP78 gene

1989 • 684 citations

The UNC operon nucleotide sequence, regulation and structure of ATP-synthase

1984 • 519 citations

Molecular structure of a gene, VMA1, encoding the catalytic subunit of H(+)-translocating adenosine triphosphatase from vacuolar membranes of Saccharomyces cerevisiae.

1990 • 474 citations

[2] Production of antisera with small doses of immunogen: multiple intradermal injections

1981 • 442 citations

Retrieval of transmembrane proteins to the endoplasmic reticulum.

1993 • 414 citations

PEP4 gene of Saccharomyces cerevisiae encodes proteinase A, a vacuolar enzyme required for processing of vacuolar precursors.

1986 • 400 citations

[44] Methods for studying the yeast vacuole

1991 • 346 citations

Membrane protein sorting: biosynthesis, transport and processing of yeast vacuolar alkaline phosphatase.

1989 • 330 citations

The cyclophilin homolog ninaA is required in the secretory pathway

1991 • 330 citations

Purification and properties of H+-translocating, Mg2+-adenosine triphosphatase from vacuolar membranes of Saccharomyces cerevisiae.

1985 • 316 citations

The PEP4 Gene Encodes an Aspartyl Protease Implicated in the Posttranslational Regulation of Saccharomyces cerevisiae Vacuolar Hydrolases

1986 • 292 citations

Folding, trimerization, and transport are sequential events in the biogenesis of influenza virus hemagglutinin

1988 • 285 citations

The VPH1 gene encodes a 95-kDa integral membrane polypeptide required for in vivo assembly and activity of the yeast vacuolar H(+)-ATPase.

1992 • 258 citations

Mutation of a tyrosine localization signal in the cytosolic tail of yeast Kex2 protease disrupts Golgi retention and results in default transport to the vacuole.

1992 • 209 citations

SHR3: A novel component of the secretory pathway specifically required for localization of amino acid permeases in yeast

1992 • 200 citations

Role of Vacuolar Acidification in Protein Sorting and Zymogen Activation: a Genetic Analysis of the Yeast Vacuolar Proton-Translocating ATPase

1990 • 190 citations

Biochemical characterization of the yeast vacuolar H+-ATPase

1989 • 182 citations

Assembly and targeting of peripheral and integral membrane subunits of the yeast vacuolar H(+)-ATPase.

1992 • 180 citations

Membrane protein sorting in the yeast secretory pathway: evidence that the vacuole may be the default compartment.

1992 • 179 citations

The progenitor of ATP synthases was closely related to the current vacuolar H⁺‐ATPase

1989 • 161 citations

VMA13 encodes a 54-kDa vacuolar H(+)-ATPase subunit required for activity but not assembly of the enzyme complex in Saccharomyces cerevisiae.

1993 • 153 citations

Structure, biosynthesis, and localization of dipeptidyl aminopeptidase B, an integral membrane glycoprotein of the yeast vacuole.

1989 • 152 citations

The yeast EUG1 gene encodes an endoplasmic reticulum protein that is functionally related to protein disulfide isomerase.

1992 • 150 citations

A Conserved Gene Encoding the 57-kDa Subunit of the Yeast Vacuolar H+-ATPase

1989 • 144 citations

The Saccharomyces cerevisiae VMA6 gene encodes the 36-kDa subunit of the vacuolar H(+)-ATPase membrane sector

1993 • 133 citations

Isolation of vacuolar membrane H(+)-ATPase-deficient yeast mutants; the VMA5 and VMA4 genes are essential for assembly and activity of the vacuolar H(+)-ATPase.

1993 • 127 citations

Protein splicing of the yeast TFP1 intervening protein sequence: a model for self-excision.

1993 • 125 citations

Structural and functional dissection of Sec62p, a membrane-bound component of the yeast endoplasmic reticulum protein import machinery.

1990 • 121 citations

The 31-kDa polypeptide is an essential subunit of the vacuolar ATPase in Saccharomyces cerevisiae.

1990 • 105 citations

Partial assembly of the yeast vacuolar H(+)-ATPase in mutants lacking one subunit of the enzyme

1993 • 104 citations

VMA11, a novel gene that encodes a putative proteolipid, is indispensable for expression of yeast vacuolar membrane H(+)-ATPase activity.

1991 • 101 citations

Role of vacuolar acidification in protein sorting and zymogen activation: a genetic analysis of the yeast vacuolar proton-translocating ATPase.

1990 • 96 citations

PEP4 Gene of Saccharomyces cerevisiae Encodes Proteinase A, a Vacuolar Enzyme Required for Processing of Vacuolar Precursors

1986 • 94 citations

Yeast Kex1p is a Golgi-associated membrane protein: deletions in a cytoplasmic targeting domain result in mislocalization to the vacuolar membrane.

1992 • 92 citations

Proteinase C (carboxypeptidase Y) mutant of yeast

1975 • 91 citations

Acidification of the Endocytic and Exocytic Pathways

1986 • 88 citations

A dominant trifluoperazine resistance gene from Saccharomyces cerevisiae has homology with F0F1 ATP synthase and confers calcium-sensitive growth.

1988 • 82 citations

Cloning and mutational analysis of the gene encoding subunit C of yeast vacuolar H(+)-ATPase.

1992 • 79 citations

VMA12 is essential for assembly of the vacuolar H(+)-ATPase subunits onto the vacuolar membrane in Saccharomyces cerevisiae.

1993 • 75 citations

An essential 45 kDa yeast transmembrane protein reacts with anti-nuclear pore antibodies: purification of the protein, immunolocalization and cloning of the gene.

1991 • 72 citations

Subunit composition, biosynthesis, and assembly of the yeast vacuolar proton-translocating ATPase

1992 • 69 citations

Structure and properties of the coated vesicle (H+)-ATPase

1992 • 57 citations

Structural and Functional Dissection of Sec62p, a Membrane-Bound Component of the Yeast Endoplasmic Reticulum Protein Import Machinery

1990 • 51 citations

The Yeast EUG1 Gene Encodes an Endoplasmic Reticulum Protein That Is Functionally Related to Protein Disulfide Isomerase

1992 • 35 citations

An essential 45 kDa yeast transmembrane protein reacts with anti-nuclear pore antibodies: purification of the protein, immunolocalization and cloning of the gene.

1991 • 24 citations

A Dominant Trifluoperazine Resistance Gene from Saccharomyces cerevisiae Has Homology with F₀F₁ ATP Synthase and Confers Calcium-Sensitive Growth

1988 • 16 citations