Biochemical characterization of the yeast vacuolar H+-ATPase

PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT

1951 • 319,299 citations

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

“Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A

1981 • 8,657 citations

ACIDIFICATION OF THE ENDOCYTIC AND EXOCYTIC PATHWAYS

1986 • 1,977 citations

Isolation of intracellular membranes by means of sodium carbonate treatment: application to endoplasmic reticulum.

1982 • 1,765 citations

Bafilomycins: a class of inhibitors of membrane ATPases from microorganisms, animal cells, and plant cells.

1988 • 1,737 citations

Protein sorting in yeast: Mutants defective in vacuole biogenesis mislocalize vacuolar proteins into the late secretory pathway

1986 • 429 citations

Purification and properties of H+-translocating, Mg2+-adenosine triphosphatase from vacuolar membranes of Saccharomyces cerevisiae.

1985 • 316 citations

Structure and function of proton-translocating adenosine triphosphatase (F0F1): biochemical and molecular biological approaches

1983 • 310 citations

Topography and subunit stoichiometry of the coated vesicle proton pump.

1988 • 280 citations

The cDNA sequence of the 69-kDa subunit of the carrot vacuolar H+-ATPase. Homology to the beta-chain of F0F1-ATPases.

1988 • 277 citations

Characterization of genes required for protein sorting and vacuolar function in the yeast Saccharomyces cerevisiae.

1989 • 258 citations

Structure of the novel membrane-coating material in proton-secreting epithelial cells and identification as an H+ATPase.

1987 • 256 citations

Active transport of basic amino acids driven by a proton motive force in vacuolar membrane vesicles of Saccharomyces cerevisiae.

1981 • 250 citations

Isolation of genes encoding the Neurospora vacuolar ATPase. Analysis of vma-1 encoding the 67-kDa subunit reveals homology to other ATPases.

1988 • 236 citations

A possible role for Na+,K+-ATPase in regulating ATP-dependent endosome acidification.

1989 • 229 citations

Regulation of endocytic pH by the Na+,K+-ATPase in living cells.

1989 • 228 citations

Cold Inactivation of Vacuolar Proton-ATPases

1989 • 203 citations

Immunoaffinity purification and characterization of vacuolar H+ATPase from bovine kidney.

1987 • 202 citations

H+-ATPases from mitochondria, plasma membranes, and vacuoles of fungal cells

1986 • 197 citations

Isolation of genes encoding the Neurospora vacuolar ATPase. Analysis of vma-2 encoding the 57-kDa polypeptide and comparison to vma-1.

1988 • 177 citations

Targeting proteins into mitochondria

1986 • 171 citations

Identification of 3-O-(4-benzoyl)benzoyladenosine 5‘-triphosphate- and N,N‘-dicyclohexylcarbodiimide-binding subunits of a higher plant H+-translocating tonoplast ATPase.

1985 • 169 citations

Properties of the partially purified tonoplast H+-pumping ATPase from oat roots.

1986 • 169 citations

Isolation and reconstitution of the clathrin-coated vesicle proton translocating complex.

1986 • 168 citations

The purified ATPase from chromaffin granule membranes is an anion-dependent proton pump.

1987 • 165 citations

Structure, biosynthesis, and localization of dipeptidyl aminopeptidase B, an integral membrane glycoprotein of the yeast vacuole.

1989 • 152 citations

cDNA sequence and homologies of the “57-kDa” nucleotide-binding subunit of the vacuolar ATPase from Arabidopsis.

1988 • 148 citations

Particle concentration fluorescence immunoassay (PCFIA): a new, rapid immunoassay technique with high sensitivity

1984 • 129 citations

Subunit composition and ATP site labeling of the coated vesicle proton-translocating adenosine triphosphatase

1987 • 119 citations

Inhibition of the coated vesicle proton pump and labeling of a 17,000-dalton polypeptide by N,N'-dicyclohexylcarbodiimide.

1987 • 115 citations

Structural studies of the vacuolar membrane ATPase from Neurospora crassa and comparison with the tonoplast membrane ATPase from Zea mays.

1986 • 115 citations

The membrane-associated ATPase from Sulfolobus acidocaldarius is distantly related to F1-ATPase as assessed from the primary structure of its alpha-subunit.

1988 • 112 citations

Partial Purification of a Tonoplast ATPase from Corn Coleoptiles

1985 • 108 citations

Characterization of the subunit structure of the maize tonoplast ATPase. Immunological and inhibitor binding studies.

1986 • 108 citations

Isolation and reconstitution of the dicyclohexylcarbodiimide-sensitive proton pore of the clathrin-coated vesicle proton translocating complex.

1987 • 105 citations

Interaction of anions and ATP with the coated vesicle proton pump

1989 • 102 citations

Nucleotide binding sites and chemical modification of the chromaffin granule proton ATPase.

1987 • 98 citations

Purification of N-ethylmaleimide-sensitive ATPase from chromaffin granule membranes.

1986 • 94 citations

Characterization and function of catalytic subunit alpha of H+-translocating adenosine triphosphatase from vacuolar membranes of Saccharomyces cerevisiae. A study with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole.

1988 • 90 citations

Cryoelectron microscopy of Escherichia coli F1 adenosinetriphosphatase decorated with monoclonal antibodies to individual subunits of the complex

1989 • 80 citations

Characterization and function of catalytic subunit alpha of H+-translocating adenosine triphosphatase from vacuolar membranes of Saccharomyces cerevisiae. A study with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole.

1988 • 78 citations

Purification and properties of the ATphase Solubilized from Membranes of an Acidothermophilic Archaeobacterium, sulfolobus acidocaldarius1

1987 • 74 citations

Peripheral and integral subunits of the tonoplast H+-ATPase from oat roots.

1988 • 72 citations

Acidification of the lysosome-like vacuole and the vacuolar H+-ATPase are deficient in two yeast mutants that fail to sort vacuolar proteins.

1989 • 72 citations

Irreversible inhibition of H+-ATPase of higher plant tonoplast by chaotropic anions: evidence for peripheral location of nucleotide-binding subunits

1987 • 69 citations

Modification of the F0 portion of ECF1-F0 by the water-soluble carbodiimide EDC and effect on the proton channeling function

1984 • 65 citations

Production and characterization of a monoclonal antibody to vacuolar H+ATPase of renal epithelia.

1987 • 57 citations

N,N'-dicyclohexylcarbodiimide-binding proteolipid of the vacuolar H+-ATPase from oat roots.

1988 • 49 citations

A new X‐prolyl‐dipeptidyl aminopeptidase from yeast associated with a particulate fraction

1981 • 48 citations

Purification of the N,N'-dicyclohexylcarbodiimide-binding proteolipid of a higher plant tonoplast H+-ATPase.

1987 • 45 citations

Efficient hybridoma production using previously frozen splenocytes

1988 • 42 citations

Purification and reconstitution of the proton-translocating ATPase of Golgi-enriched membranes.

1988 • 34 citations

Polymorphism and conformational dynamics of F1-ATPases from bacterial membranes a model for the regulation of these enzymes on the basis of molecular plasticity

1982 • 29 citations

Evolution of vacuolar H⁺-ATPases: immunological relationships of the nucleotide-binding subunits

1989 • 16 citations