VMA12 is essential for assembly of the vacuolar H(+)-ATPase subunits onto the vacuolar membrane in Saccharomyces cerevisiae.

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

A simple method for displaying the hydropathic character of a protein

1982 • 22,471 citations

A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.

1989 • 8,705 citations

Transformation of intact yeast cells treated with alkali cations

1983 • 7,688 citations

Current protocols in molecular biology

1988 • 7,584 citations

Rapid and Sensitive Protein Similarity Searches

1985 • 4,091 citations

[12] One-step gene disruption in yeast

1983 • 3,302 citations

[1] Getting started with yeast

1991 • 2,456 citations

DNA sequence analysis with a modified bacteriophage T7 DNA polymerase.

1987 • 2,266 citations

The fungal vacuole: composition, function, and biogenesis

1990 • 758 citations

Structure and function of vacuolar class of ATP-driven proton pumps

1989 • 600 citations

Molecular structure of a gene, VMA1, encoding the catalytic subunit of H(+)-translocating adenosine triphosphatase from vacuolar membranes of Saccharomyces cerevisiae.

1990 • 474 citations

[2] Production of antisera with small doses of immunogen: multiple intradermal injections

1981 • 442 citations

Protein sorting in yeast: Mutants defective in vacuole biogenesis mislocalize vacuolar proteins into the late secretory pathway

1986 • 429 citations

Organelle assembly in yeast: characterization of yeast mutants defective in vacuolar biogenesis and protein sorting.

1988 • 409 citations

Yeast Saccharomyces cerevisiae selectable markers in pUC18 polylinkers

1990 • 376 citations

A rapid, efficient method for isolating DNA from yeast

1986 • 358 citations

A simple and rapid method for generating a deletion by PCR

1991 • 340 citations

Disruption of genes encoding subunits of yeast vacuolar H(+)-ATPase causes conditional lethality.

1990 • 320 citations

Purification and properties of H+-translocating, Mg2+-adenosine triphosphatase from vacuolar membranes of Saccharomyces cerevisiae.

1985 • 316 citations

The evolution of H+-ATPases

1989 • 313 citations

Transformation of yeast spheroplasts without cell fusion

1987 • 266 citations

Characterization of genes required for protein sorting and vacuolar function in the yeast Saccharomyces cerevisiae.

1989 • 258 citations

Calcium transport driven by a proton motive force in vacuolar membrane vesicles of Saccharomyces cerevisiae.

1983 • 252 citations

Active transport of basic amino acids driven by a proton motive force in vacuolar membrane vesicles of Saccharomyces cerevisiae.

1981 • 250 citations

Properties of H+-translocating adenosine triphosphatase in vacuolar membranes of SAccharomyces cerevisiae.

1981 • 239 citations

Multiple methods of visualizing the yeast vacuole permit evaluation of its morphology and inheritance during the cell cycle.

1987 • 224 citations

Calcium-sensitive cls mutants of Saccharomyces cerevisiae showing a Pet- phenotype are ascribable to defects of vacuolar membrane H(+)-ATPase activity

1991 • 191 citations

Role of Vacuolar Acidification in Protein Sorting and Zymogen Activation: a Genetic Analysis of the Yeast Vacuolar Proton-Translocating ATPase

1990 • 190 citations

Biochemical characterization of the yeast vacuolar H+-ATPase

1989 • 182 citations

Assembly and targeting of peripheral and integral membrane subunits of the yeast vacuolar H(+)-ATPase.

1992 • 180 citations

THE SYNTHESIS AND FUNCTION OF PROTEASES IN SACCHAROMYCES: GENETIC APPROACHES

1984 • 179 citations

Cytochrome oxidase assembly in yeast requires the product of COX11, a homolog of the P. denitrificans protein encoded by ORF3.

1990 • 172 citations

The progenitor of ATP synthases was closely related to the current vacuolar H⁺‐ATPase

1989 • 161 citations

Structure, biosynthesis, and localization of dipeptidyl aminopeptidase B, an integral membrane glycoprotein of the yeast vacuole.

1989 • 152 citations

Mutational analysis of yeast vacuolar H(+)-ATPase.

1991 • 152 citations

Identification of two nuclear genes (ATP11, ATP12) required for assembly of the yeast F1-ATPase.

1990 • 151 citations

A Conserved Gene Encoding the 57-kDa Subunit of the Yeast Vacuolar H+-ATPase

1989 • 144 citations

Substrate specificities of active transport systems for amino acids in vacuolar-membrane vesicles of Saccharomyces cerevisiae. Evidence of seven independent proton/amino acid antiport systems.

1984 • 132 citations

Roles of the VMA3 gene product, subunit c of the vacuolar membrane H(+)-ATPase on vacuolar acidification and protein transport. A study with VMA3-disrupted mutants of Saccharomyces cerevisiae.

1990 • 131 citations

The SLP1 gene of Saccharomyces cerevisiae is essential for vacuolar morphogenesis and function.

1990 • 123 citations

Molecular analysis of the yeast VPS3 gene and the role of its product in vacuolar protein sorting and vacuolar segregation during the cell cycle.

1990 • 112 citations

The 31-kDa polypeptide is an essential subunit of the vacuolar ATPase in Saccharomyces cerevisiae.

1990 • 105 citations

Nucleotide sequence ofAMS1, the structure gene of vacuolarα-mannosidase of Saccharomyces cerevisiae

1989 • 102 citations

VMA11, a novel gene that encodes a putative proteolipid, is indispensable for expression of yeast vacuolar membrane H(+)-ATPase activity.

1991 • 101 citations

ATP10, a yeast nuclear gene required for the assembly of the mitochondrial F1-F0 complex.

1990 • 100 citations

Role of vacuolar acidification in protein sorting and zymogen activation: a genetic analysis of the yeast vacuolar proton-translocating ATPase.

1990 • 96 citations

Characterization and function of catalytic subunit alpha of H+-translocating adenosine triphosphatase from vacuolar membranes of Saccharomyces cerevisiae. A study with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole.

1988 • 90 citations

Isolation and Characterization of Ca2+-sensitive Mutants of Saccharomyces cerevisiae

1986 • 79 citations

Cloning and mutational analysis of the gene encoding subunit C of yeast vacuolar H(+)-ATPase.

1992 • 79 citations

Characterization and function of catalytic subunit alpha of H+-translocating adenosine triphosphatase from vacuolar membranes of Saccharomyces cerevisiae. A study with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole.

1988 • 78 citations

Mutants of Saccharomyces cerevisiae with defective vacuolar function

1988 • 72 citations

Genetic and cell biological aspects of the yeast vacuolar H+-ATPase

1992 • 72 citations

Characterization of the END1 gene required for vacuole biogenesis and gluconeogenic growth of budding yeast.

1989 • 72 citations

Subunit composition, biosynthesis, and assembly of the yeast vacuolar proton-translocating ATPase

1992 • 69 citations

Characterization of ATP12, a yeast nuclear gene required for the assembly of the mitochondrial F1-ATPase.

1991 • 61 citations

Recombinant DNA techniques: An introduction

1984 • 55 citations

Characterization of ATP11 and detection of the encoded protein in mitochondria of Saccharomyces cerevisiae.

1992 • 55 citations

A new X‐prolyl‐dipeptidyl aminopeptidase from yeast associated with a particulate fraction

1981 • 48 citations

ATP13, a nuclear gene of Saccharomyces cerevisiae essential for the expression of subunit 9 of the mitochondrial ATPase

1991 • 47 citations

Genes required for vacuolar acidity in Saccharomyces cerevisiae.

1992 • 38 citations

Functional molecular masses of vacuolar membrane H⁺‐ATPase from Saccharomyces cerevisiae as studied by radiation inactivation analysis

1989 • 23 citations

A multinuclear magnetic resonance study of a cls11 mutant showing the Pet⁻ phenotype of Saccharomyces cerevisiae

1990 • 5 citations