mRNA destabilization triggered by premature translational termination depends on at least three cis-acting sequence elements and one trans-acting factor.

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Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.

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Transformation of intact yeast cells treated with alkali cations

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A conserved AU sequence from the 3′ untranslated region of GM-CSF mRNA mediates selective mRNA degradation

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High efficiency transformation of intact yeast cells using single stranded nucleic acids as a carrier

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Laboratory course manual for methods in yeast genetics

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Compilation of small ribosomal subunit RNA sequences

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The product of the yeast UPF1 gene is required for rapid turnover of mRNAs containing a premature translational termination codon.

1991 • 487 citations

Interference of nonsense mutations with eukaryotic messenger RNA stability.

1979 • 454 citations

Identification and comparison of stable and unstable mRNAs in Saccharomyces cerevisiae.

1990 • 409 citations

Nonsense mutations in the dihydrofolate reductase gene affect RNA processing.

1989 • 367 citations

Eucaryotic RNA Polymerase Conditional Mutant that Rapidly Ceases mRNA Synthesis

1987 • 353 citations

Translation is required for regulation of histone mRNA degradation

1987 • 345 citations

Gene products that promote mRNA turnover in Saccharomyces cerevisiae.

1992 • 330 citations

Control of c-myc mRNA half-life in vitro by a protein capable of binding to a coding region stability determinant.

1992 • 301 citations

The protein-coding region of c-myc mRNA contains a sequence that specifies rapid mRNA turnover and induction by protein synthesis inhibitors.

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Unstable β-globin mRNA in mRNA-deficient β0 thalassemia

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The functional role of ribosomal RNA in protein synthesis

1989 • 238 citations

Premature translation termination mediates triosephosphate isomerase mRNA degradation.

1988 • 229 citations

Compilation of small ribosomal subunit RNA sequences

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Nonsense codons can reduce the abundance of nuclear mRNA without affecting the abundance of pre-mRNA or the half-life of cytoplasmic mRNA.

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Consequences of frameshift mutations at the immunoglobulin heavy chain locus of the mouse.

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Cycloheximide resistance in yeast: the gene and its portein

1983 • 188 citations

The primary structure of theSaccharomyces cerevisiaegene for 3-phosphogrycerate kinase

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Translation to near the distal end of the penultimate exon is required for normal levels of spliced triosephosphate isomerase mRNA.

1990 • 159 citations

A single base deletion in the Tfm androgen receptor gene creates a short-lived messenger RNA that directs internal translation initiation.

1991 • 151 citations

Polarity and the Degradation of mRNA

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Translation and a 42-nucleotide segment within the coding region of the mRNA encoded by the MAT alpha 1 gene are involved in promoting rapid mRNA decay in yeast.

1990 • 138 citations

Sequences that confer β-tubulin autoregulation through modulated mRNA stability reside within exon 1 of a β-tubulin mRNA

1987 • 129 citations

Effect of premature termination of translation on mRNA stability depends on the site of ribosome release.

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Beta-globin nonsense mutation: deficient accumulation of mRNA occurs despite normal cytoplasmic stability.

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[29] Measurement of mRNA decay rates in Saccharomyces cerevisiae

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Determinants and regulation of cytoplasmic mRNA stability in eukaryotic cells

1991 • 119 citations

Nonsense Codons in Human β-Globin mRNA Result in the Production of mRNA Degradation Products

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Multiple determinants of eukaryotic mRNA stability.

1989 • 118 citations

A new group of putative RNA helicases

1992 • 99 citations

Mutations affecting the tRNA-splicing endonuclease activity of Saccharomyces cerevisiae.

1988 • 95 citations

NAM7 nuclear gene encodes a novel member of a family of helicases with a Zn-ligand motif and is involved in mitochondrial functions in Saccharomyces cerevisiae

1992 • 91 citations

A mutation in the tRNA nucleotidyltransferase gene promotes stabilization of mRNAs in Saccharomyces cerevisiae.

1992 • 87 citations

Nonsense codons within the Rous sarcoma virus gag gene decrease the stability of unspliced viral RNA.

1991 • 72 citations

Analysis of chimeric mRNAs derived from theSTE3mRNA identifies multiple regions within yeast mRNAs that modulate mRNA decay

1992 • 56 citations

Nonsense suppressors partially revert the decrease of the mRNA level of a nonsense mutant allele in yeast

1990 • 52 citations

mRNA Turnover in Saccharomyces cerevisiae

1993 • 52 citations

Picornavirus RNA translation continues to surprise

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Effect of ochre nonsense mutations on yeast URA1 mRNA stability

1984 • 42 citations

A coding region segment is necessary, but not sufficient for rapid decay of the HIS3 mRNA in yeast

1992 • 39 citations

Identification of the cis-acting sequences and trans-acting factors involved in nonsense-mediated mRNA decay

1993 • 32 citations

‘Omnipotent’ nonsense suppressors: new clues to an old puzzle

1988 • 12 citations

Saccharomyces cerevisiae mRNA populations of different intrinsic stability in unstressed and heat shocked cells display almost constant m⁷GpppA:m⁷GpppG 5′‐cap structure ratios

1987 • 7 citations

Regulation of mRNA Stability in Yeast

1990 • 7 citations