Isolation and characterization of cDNA clones for rat ribophorin I: complete coding sequence and in vitro synthesis and insertion of the encoded product into endoplasmic reticulum membranes

Molecular Cloning: A Laboratory Manual

2001 • 133,517 citations

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

1979 • 54,907 citations

Short-Term Effects of Nose-Only Cigarette Smoke Exposure on Glutathione Redox Homeostasis, Cytochrome P450 1A1/2 and Respiratory Enzyme Activities in Mice Tissues

2013 • 47,043 citations

Detection of specific sequences among DNA fragments separated by gel electrophoresis

1975 • 33,059 citations

Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease

1979 • 22,482 citations

[57] Sequencing end-labeled DNA with base-specific chemical cleavages

1980 • 15,302 citations

Efficientin vitrosynthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter

1984 • 6,459 citations

Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes

1986 • 4,785 citations

A simple and very efficient method for generating cDNA libraries

1983 • 4,606 citations

Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins

1978 • 4,566 citations

Rapid and Sensitive Protein Similarity Searches

1985 • 4,091 citations

Prediction of protein antigenic determinants from amino acid sequences.

1981 • 3,715 citations

Transfer of proteins across membranes. I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of murine myeloma.

1975 • 3,026 citations

3′ Non-coding region sequences in eukaryotic messenger RNA

1976 • 3,001 citations

A system for shotgun DNA sequencing

1981 • 2,710 citations

Patterns of Amino Acids near Signal‐Sequence Cleavage Sites

1983 • 2,290 citations

Cytoplasmic dot hybridization. Simple analysis of relative mRNA levels in multiple small cell or tissue samples.

1982 • 1,333 citations

Transfer of proteins across membranes. II. Reconstitution of functional rough microsomes from heterologous components.

1975 • 1,175 citations

A putative signal peptidase recognition site and sequence in eukaryotic and prokaryotic signal peptides

1983 • 1,143 citations

Yeast RNA Polymerase II Genes: Isolation with Antibody Probes

1983 • 1,084 citations

Transfer of Proteins Across Membranes

1981 • 894 citations

Mechanisms for the incorporation of proteins in membranes and organelles.

1982 • 875 citations

Homologies in both primary and secondary structure between nuclear envelope and intermediate filament proteins

1986 • 836 citations

Protein translocation across the endoplasmic reticulum

1984 • 825 citations

Translocation of proteins across the endoplasmic reticulum III. Signal recognition protein (SRP) causes signal sequence-dependent and site-specific arrest of chain elongation that is released by microsomal membranes.

1981 • 807 citations

Multiple Mechanisms of Protein Insertion into and Across Membranes

1985 • 720 citations

Three different fibronectin mRNAs arise by alternative splicing within the coding region

1983 • 713 citations

[6] Preparation of microsomal membranes for cotranslational protein translocation

1983 • 679 citations

Conformational preferences of amino acids in globular proteins

1978 • 653 citations

Secretory protein translocation across membranes—the role of the ‘docking protein’

1982 • 648 citations

Translocation of proteins across the endoplasmic reticulum. I. Signal recognition protein (SRP) binds to in-vitro-assembled polysomes synthesizing secretory protein.

1981 • 644 citations

Purification of a membrane-associated protein complex required for protein translocation across the endoplasmic reticulum.

1980 • 502 citations

Protein translocation across the endoplasmic reticulum. II. Isolation and characterization of the signal recognition particle receptor.

1982 • 478 citations

Synthesis and incorporation of myelin polypeptides into CNS myelin.

1982 • 422 citations

Purification of microsomal signal peptidase as a complex.

1986 • 313 citations

The cDNA sequence of a human epidermal keratin: Divergence of sequence but conservation of structure among intermediate filament proteins

1982 • 298 citations

Proteins of rough microsomal membranes related to ribosome binding. I. Identification of ribophorins I and II, membrane proteins characteristics of rough microsomes

1978 • 281 citations

Ribosome-Membrane Interaction in Eukaryotic Cells

1971 • 278 citations

Identification of Human Glucocorticoid Receptor Complementary DNA Clones by Epitope Selection

1985 • 277 citations

Post-translational cleavage of presecretory proteins with an extract of rough microsomes from dog pancreas containing signal peptidase activity.

1977 • 259 citations

The Function of Saccharide-Lipids in Synthesis of Glycoproteins

1980 • 247 citations

Antiparallel and parallel β-strands differ in amino acid residue preferences

1979 • 206 citations

On the Hydrophobic Nature of Signal Sequences

1981 • 193 citations

A comparison of the amino acid sequences of rabbit skeletal muscle alpha- and beta-tropomyosins.

1980 • 185 citations

Complete cDNA and protein sequence of a pregnenolone 16 alpha-carbonitrile-induced cytochrome P-450. A representative of a new gene family.

1985 • 181 citations

Molecular structure and polymorphic map of the human phenylalanine hydroxylase gene

1986 • 174 citations

Rat liver UDP-glucuronosyltransferase. Sequence and expression of a cDNA encoding a phenobarbital-inducible form.

1986 • 171 citations

Transient involvement of signal recognition particle and its receptor in the microsomal membrane prior to protein translocation

1983 • 160 citations

Proteins of rough microsomal membranes related to ribosome binding. II. Cross-linking of bound ribosomes to specific membrane proteins exposed at the binding sites

1978 • 142 citations

Topology of signal recognition particle receptor in endoplasmic reticulum membrane

1985 • 140 citations

Identification and characterization of a membrane component essential for the translocation of nascent proteins across the membrane of the endoplasmic reticulum.

1980 • 137 citations

Characterization of secretory protein translocation: ribosome-membrane interaction in endoplasmic reticulum.

1986 • 121 citations

Primary and secondary structure of hamster vimentin predicted from the nucleotide sequence.

1983 • 121 citations

Sequence repeats in α-tropomyosin

1975 • 114 citations

Protein transfer across microsomal membranes reassembled from separated membrane components

1978 • 114 citations

Biosynthesis and processing of ribophorins in the endoplasmic reticulum.

1984 • 83 citations

Segregation of the polypeptide translocation apparatus to regions of the endoplasmic reticulum containing ribophorins and ribosomes. II. Rat liver microsomal subfractions contain equimolar amounts of ribophorins and ribosomes.

1984 • 80 citations

[35] Second-strand cDNA synthesis: mRNA fragments as primers

1987 • 71 citations

Identification of Ribophorins in Rough Microsomal Membranes from Different Organs of Several Species

1982 • 40 citations

Electrophoretic studies on liver endoplasmic reticulum membrane polypeptides and on their phosphorylation in vivo and in vitro.

1978 • 37 citations

Pushing the signal hypothesis: what are the limits?

1985 • 37 citations

Discrimination among multiple AATAAA sequences correlates with inter-species conservation of select 3'untranslated nucleotides

1986 • 25 citations

Biosynthesis of hepatocyte endoplasmic reticulum proteins

1983 • 17 citations