Gene targeting at the mouse cytokeratin 10 locus: severe skin fragility and changes of cytokeratin expression in the epidermis.

Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction

1987 • 64,270 citations

THE USE OF LEAD CITRATE AT HIGH pH AS AN ELECTRON-OPAQUE STAIN IN ELECTRON MICROSCOPY

1963 • 24,942 citations

The catalog of human cytokeratins: Patterns of expression in normal epithelia, tumors and cultured cells

1982 • 5,272 citations

Transmission Electron Microscopy

2009 • 3,700 citations

Embedding in Epoxy Resins for Ultrathin Sectioning in Electron Microscopy

1960 • 3,319 citations

INTERMEDIATE FILAMENTS: Structure, Dynamics, Function and Disease

1994 • 1,408 citations

Functional inactivation of genes by dominant negative mutations

1987 • 1,174 citations

Changes in keratin gene expression during terminal differentiation of the keratinocyte

1980 • 1,059 citations

Point mutations in human keratin 14 genes of epidermolysis bullosa simplex patients: Genetic and functional analyses

1991 • 647 citations

Diversity of cytokeratins

1981 • 611 citations

Mice lacking vimentin develop and reproduce without an obvious phenotype

1994 • 603 citations

Monoclonal antibody analysis of keratin expression in epidermal diseases: a 48- and 56-kdalton keratin as molecular markers for hyperproliferative keratinocytes.

1984 • 567 citations

Programming gene expression in developing epidermis

1994 • 487 citations

Epidermolysis Bullosa Simplex: Evidence in Two Families for Keratin Gene Abnormalities

1991 • 426 citations

Mutant keratin expression in transgenic mice causes marked abnormalities resembling a human genetic skin disease

1991 • 413 citations

A mutation in the conserved helix termination peptide of keratin 5 in hereditary skin blistering

1992 • 406 citations

Antibody markers of basal cells in complex epithelia

1990 • 382 citations

Changes in Keratinocyte Maturation During Wound Healing

1987 • 375 citations

Mutations in the Rod Domains of Keratins 1 and 10 in Epidermolytic Hyperkeratosis

1992 • 370 citations

Formation of Cytoskeletal Elements During Mouse Embryogenesis

1980 • 346 citations

Pair formation and promiscuity of cytokeratins: formation in vitro of heterotypic complexes and intermediate-sized filaments by homologous and heterologous recombinations of purified polypeptides.

1985 • 343 citations

The genetic basis of epidermolytic hyperkeratosis: A disorder of differentiation-specific epidermal keratin genes

1992 • 339 citations

Mice lacking glial fibrillary acidic protein display astrocytes devoid of intermediate filaments but develop and reproduce normally.

1995 • 334 citations

Keratin Antigens in Differentiating Skin

1985 • 330 citations

Keratin 16 and keratin 17 mutations cause pachyonychia congenita

1995 • 322 citations

Formation of cytoskeletal elements during mouse embryogenesis. IV. Ultrastructure of primary mesenchymal cells and their cell-cell interactions.

1983 • 320 citations

Colorectal hyperplasia and inflammation in keratin 8-deficient FVB/N mice.

1994 • 304 citations

A leucine→proline mutation in the H1 subdomain of keratin 1 causes epidermolytic hyperkeratosis

1992 • 284 citations

The expression of keratin genes in epidermis and cultured epidermal cells

1978 • 264 citations

The basal keratin network of stratified squamous epithelia: defining K15 function in the absence of K14.

1995 • 263 citations

Mice devoid of the glial fibrillary acidic protein develop normally and are susceptible to scrapie prions

1995 • 258 citations

A new mouse embryonic stem cell line with good germ line contribution and gene targeting frequency

1992 • 249 citations

Intermediate filaments in disease

1995 • 248 citations

Mid-gestational lethality in mice lacking keratin 8.

1993 • 247 citations

Keratin 9 gene mutations in epidermolytic palmoplantar keratoderma (EPPK)

1994 • 244 citations

Elucidating the early stages of keratin filament assembly.

1990 • 243 citations

The coiled coil of in vitro assembled keratin filaments is a heterodimer of type I and II keratins: use of site-specific mutagenesis and recombinant protein expression.

1990 • 239 citations

The expression of mutant epidermal keratin cDNAs transfected in simple epithelial and squamous cell carcinoma lines.

1987 • 237 citations

Mutation of a type II keratin gene (K6a) in pachyonychia congenita

1995 • 230 citations

Ichthyosiform Dermatoses

1966 • 213 citations

Expression of mutant keratin cDNAs in epithelial cells reveals possible mechanisms for initiation and assembly of intermediate filaments.

1989 • 210 citations

Epidermal cytokines and their roles in cutaneous wound healing

1991 • 204 citations

A function for keratins and a common thread among different types of epidermolysis bullosa simplex diseases.

1991 • 202 citations

A human keratin 14 "knockout": the absence of K14 leads to severe epidermolysis bullosa simplex and a function for an intermediate filament protein.

1994 • 200 citations

Epidermal growth factor and transforming growth factor alpha specifically induce the activation- and hyperproliferation-associated keratins 6 and 16.

1993 • 187 citations

Transgenic mice expressing a mutant keratin 10 gene reveal the likely genetic basis for epidermolytic hyperkeratosis.

1992 • 178 citations

A functional "knockout" of human keratin 14.

1994 • 176 citations

Intermediate Filaments: A Review

2011 • 169 citations

Retrovirus-mediated transgenic keratin expression in cultured fibroblasts: Specific domain functions in keratin stabilization and filament formation

1990 • 165 citations

Keratin alterations during embryonic epidermal differentiation: a presage of adult epidermal maturation.

1982 • 158 citations

Do the ends justify the mean? Proline mutations at the ends of the keratin coiled-coil rod segment are more disruptive than internal mutations.

1992 • 152 citations

Mutations in the rod domain of keratin 2e in patients with ichthyosis bullosa of Siemens

1994 • 145 citations

Molecular characterization and expression of the stratification-related cytokeratins 4 and 15.

1988 • 140 citations

Clinical Heterogeneity in Epidermolytic Hyperkeratosis

1994 • 139 citations

Selective Involvement of Keratins K1 and K10 in the Cytoskeletal Abnormality of Epidermolytic Hyperkeratosis (Bullous Congenital Ichthyosiform Erythroderma)

1992 • 135 citations

Characterization of human cytokeratin 2, an Epidermal cytoskeletal protein synthesized late during differentiation

1992 • 129 citations

TGF-beta and retinoic acid: regulators of growth and modifiers of differentiation in human epidermal cells.

1990 • 129 citations

The roles of K5 and K14 head, tail, and R/K L L E G E domains in keratin filament assembly in vitro.

1992 • 127 citations

Transient synthesis of K6 and K16 keratins in regenerating rabbit corneal epithelium: keratin markers for an alternative pathway of keratinocyte differentiation

1989 • 126 citations

Disease severity correlates with position of keratin point mutations in patients with epidermolysis bullosa simplex.

1993 • 122 citations

Posttranslational Regulation of Keratins: Degradation of Mouse and Human Keratins 18 and 8

1989 • 121 citations

Use of double-replacement gene targeting to replace the murine alpha-lactalbumin gene with its human counterpart in embryonic stem cells and mice.

1994 • 115 citations

Gene targeting using a mouse HPRT minigene/HPRT-deficient embryonic stem cell system: Inactivation of the mouseERCC-1 gene

1992 • 111 citations

Epidermolytic palmoplantar keratoderma cosegregates with a keratin 9 mutation in a pedigree with breast and ovarian cancer

1994 • 111 citations

Modulation of keratin intermediate filament assembly by single amino acid exchanges in the consensus sequence at the C-terminal end of the rod domain

1991 • 109 citations

Cessation of cytokeratin expression in a rat hepatoma cell line lacking differentiated functions

1983 • 106 citations

Ichthyosis Bullosa of Siemens–A Disease Involving Keratin 2e

1994 • 103 citations

Stem Cells in Hair Follicles

1991 • 98 citations

Ichthyosis Bullosa of Siemens Is Caused by Mutations in the Keratin 2e Gene

1994 • 97 citations

Gene targeting in the mouse

1994 • 97 citations

Increased expression of keratin 16 causes anomalies in cytoarchitecture and keratinization in transgenic mouse skin.

1994 • 94 citations

Organization of a type I keratin gene. Evidence for evolution of intermediate filaments from a common ancestral gene.

1985 • 91 citations

Role of Transforming Growth Factor Beta in the Maturation of Human Epidermal Keratinocytes

1988 • 82 citations

Ultrastructure of inborn errors of keratinization

1974 • 81 citations

Extensive size polymorphism of the human keratin 10 chain resides in the C-terminal V2 subdomain due to variable numbers and sizes of glycine loops.

1992 • 79 citations

Cytokeratin domains involved in heterotypic complex formation determined by in-vitro binding assays

1987 • 74 citations

Translational products of mRNAs coding for non-epidermal cytokeratins.

1983 • 71 citations

Ectopic synthesis of epidermal cytokeratins in pancreatic islet cells of transgenic mice interferes with cytoskeletal order and insulin production.

1993 • 67 citations

Analysis of cytokeratin domains by cloning and expression of intact and deleted polypeptides in Escherichia coli.

1987 • 66 citations

Keratinocyte differentiation in psoriatic scalp: morphology and expression of epithelial keratins

2006 • 62 citations

A synthetic peptide representing the consensus sequence motif at the carboxy-terminal end of the rod domain inhibits intermediate filament assembly and disassembles preformed filaments.

1992 • 61 citations

A potential role for the COOH-terminal domain in the lateral packing of type III intermediate filaments.

1991 • 58 citations

Ultrastructural Identification of Basic Abnormalities as Clues to Genetic Disorders of the Epidermis.

1994 • 58 citations

TGF-α Is Widely Expressed in Differentiated as well as Hyperproliferative Skin Epithelium

1991 • 56 citations

Intermediate filament molecular biology

1992 • 48 citations

The Two Size Alleles of Human Keratin 1 Are Due to a Deletion in the Glycine-Rich Carboxyl-Terminal V2 Subdomain

1992 • 45 citations

Electron Microscopy in the Early Diagnosis of Genetic Disorders of the Skin

1978 • 35 citations

Hypothesis: Intermediate filament and related proteins: Potential activators of nucleosomes during transcription initiation and elongation?

1994 • 32 citations

Expression of an epidermal keratin protein in liver of transgenic mice causes structural and functional abnormalities.

1995 • 32 citations

The intermediate filament system of the keratinizing mouse forestomach epithelium: Coexpression of keratins of internal squamous epithelia and of epidermal keratins in differentiating cells

1988 • 31 citations

Partial rescue of a lethal phenotype of fragile bones in transgenic mice with a chimeric antisense gene directed against a mutated collagen gene.

1994 • 30 citations

Use of Double-Replacement Gene Targeting to Replace the Murine α-Lactalbumin Gene with Its Human Counterpart in Embryonic Stem Cells and Mice

1994 • 28 citations

Murine Epidermal Keratins

1993 • 27 citations

Transgenic Models of Skin Diseases

1993 • 27 citations

De novo formation of cytokeratin filaments in calf lens cells and cytoplasts after transfection with cDNAs or microinjection with mRNAs encoding human cytokeratins.

1990 • 20 citations

Allelic Variations of Human Keratins K4 and K5 Provide Polymorphic Markers Within the Type II Keratin Gene Cluster on Chromosome 12

1993 • 20 citations