Functional inactivation of genes by dominant negative mutations

[12] One-step gene disruption in yeast

1983 • 3,302 citations

Efficient isolation of genes by using antibody probes.

1983 • 2,065 citations

Disruption of the Dictyostelium Myosin Heavy Chain Gene by Homologous Recombination

1987 • 984 citations

Insertion of DNA sequences into the human chromosomal β-globin locus by homologous recombination

1985 • 978 citations

cdc25+ functions as an inducer in the mitotic control of fission yeast

1986 • 973 citations

Functional dissection of a eukaryotic transcriptional activator protein, GCN4 of Yeast

1986 • 946 citations

Replacement of chromosome segments with altered DNA sequences constructed in vitro.

1979 • 779 citations

DNA sequences bound specifically by glucocorticoid receptor in vitro render a heterologous promoter hormone responsive in vivo

1983 • 692 citations

Inhibition of thymidine kinase gene expression by anti-sense RNA: A molecular approach to genetic analysis

1984 • 558 citations

Identification and cloning of localized maternal RNAs from xenopus eggs

1985 • 464 citations

A developmentally regulated activity that unwinds RNA duplexes

1987 • 439 citations

Homology among DNA-binding proteins suggests use of a conserved super-secondary structure

1982 • 432 citations

Injected anti-sense RNAs specifically block messenger RNA translation in vivo.

1985 • 410 citations

10-nm filaments are induced to collapse in living cells microinjected with monoclonal and polyclonal antibodies against tubulin.

1984 • 388 citations

Antisense RNA of proto-oncogene c-fos blocks renewed growth of quiescent 3T3 cells.

1987 • 381 citations

Stable reduction of thymidine kinase activity in cells expressing high levels of anti-sense RNA

1985 • 334 citations

Normal stoichiometry of histone dimer sets is necessary for high fidelity of mitotic chromosome transmission

1986 • 322 citations

Antisense RNA injections in fertilized frog eggs reveal an RNA duplex unwinding activity

1987 • 320 citations

Novel subunit—subunit interactions in the structure of glutamine synthetase

1986 • 310 citations

Tissue-specific expression of the rat pancreatic elastase I gene in transgenic mice

1984 • 239 citations

Introduction of homologous DNA sequences into mammalian cells induces mutations in the cognate gene

1986 • 236 citations

Phenocopy of discoidin I-minus mutants by antisense transformation in Dictyostelium

1985 • 225 citations

Production of phenocopies by Krüppel antisense RNA injection into Drosophila embryos

1985 • 210 citations

Gametogenesis and the early embryo

1987 • 207 citations

MUTATIONS WITH DOMINANT EFFECTS ON THE BEHAVIOR AND MORPHOLOGY OF THE NEMATODE CAENORHABDITIS ELEGANS

1986 • 197 citations

Direct and indirect gene replacements in Aspergillus nidulans.

1985 • 183 citations

Transient reversion of ras oncogene-induced cell transformation by antibodies specific for amino acid 12 of ras protein

1985 • 152 citations

Structure of the DNA-binding region of lac repressor inferred from its homology with cro repressor.

1982 • 142 citations

Molecular genetics of filamentous fungi

1985 • 140 citations

Mutational studies with the trp repressor of Escherichia coli support the helix-turn-helix model of repressor recognition of operator DNA.

1985 • 128 citations

A rat monoclonal antibody reacting specifically with the tyrosylated form of alpha-tubulin. II. Effects on cell movement, organization of microtubules, and intermediate filaments, and arrangement of Golgi elements.

1983 • 125 citations

The DNA Enzymology of Protein Machines

1984 • 115 citations

Shared active sites in oligomeric enzymes: model studies with defective mutants of aspartate transcarbamoylase produced by site-directed mutagenesis.

1987 • 101 citations

Functional domains of the yeast regulatory protein GAL4.

1986 • 95 citations

Gametogenesis and the early embryo

1986 • 91 citations

GENETIC STUDIES OF UNUSUAL LOCI THAT AFFECT BODY SHAPE OF THE NEMATODE CAENORHABDITIS ELEGANS AND MAY CODE FOR CUTICLE STRUCTURAL PROTEINS

1986 • 88 citations

Dominant mutations affecting muscle structure in Caenorhabditis elegans that map near the actin gene cluster

1984 • 84 citations

Regeneration of active enzyme by formation of hybrids from inactive derivatives: implications for active sites shared between polypeptide chains of aspartate transcarbamoylase.

1985 • 71 citations

Reversal of transformed phenotype by monoclonal antibodies against Ha-ras p21 proteins

1986 • 65 citations

THE GLN1 LOCUS OF SACCHAROMYCES CEREVISIAE ENCODES GLUTAMINE SYNTHETASE

1985 • 41 citations

HTLV x Gene Mutants Exhibit Novel Transcriptional Regulatory Phenotypes

1987 • 30 citations

Quaternary constraint in hybrid of aspartate transcarbamylase containing wild-type and mutant catalytic subunits.

1975 • 20 citations

Mutants without mutations

1985 • 9 citations