Activation of heat shock gene transcription by heat shock factor 1 involves oligomerization, acquisition of DNA-binding activity, and nuclear localization and can occur in the absence of stress.

Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.

1982 • 8,069 citations

[6] Use of T7 RNA polymerase to direct expression of cloned genes

1990 • 6,681 citations

Firefly luciferase gene: structure and expression in mammalian cells.

1987 • 3,088 citations

The estrogen receptor binds tightly to its responsive element as a ligand-induced homodimer

1988 • 1,233 citations

Yeast heat shock factor is an essential DNA-binding protein that exhibits temperature-dependent phosphorylation

1988 • 886 citations

A human beta-actin expression vector system directs high-level accumulation of antisense transcripts.

1987 • 767 citations

Key features of heat shock regulatory elements.

1988 • 566 citations

Protein Import into the Cell Nucleus

1986 • 544 citations

The heat shock response is self-regulated at both the transcriptional and posttranscriptional levels

1982 • 542 citations

A drosophila RNA polymerase II transcription factor binds to the regulatory site of an hsp 70 gene

1984 • 534 citations

The human heat shock protein hsp70 interacts with HSF, the transcription factor that regulates heat shock gene expression.

1992 • 525 citations

Nuclear and nucleolar localization of the 72,000-dalton heat shock protein in heat-shocked mammalian cells.

1984 • 522 citations

Heat shock factor is regulated differently in yeast and HeLa cells

1987 • 489 citations

Two protein-binding sites in chromatin implicated in the activation of heat-shock genes

1984 • 474 citations

Molecular cloning and expression of a human heat shock factor, HSF1.

1991 • 472 citations

Hsp70 accelerates the recovery of nucleolar morphology after heat shock.

1984 • 456 citations

Germline Transformation Used to Define Key Features of Heat-Shock Response Elements

1988 • 451 citations

Stable binding of Drosophila heat shock factor to head-to-head and tail-to-tail repeats of a conserved 5 bp recognition unit

1989 • 439 citations

Isolation of the gene encoding the S. cerevisiae heat shock transcription factor

1988 • 417 citations

Coordinate changes in heat shock element-binding activity and HSP70 gene transcription rates in human cells.

1988 • 407 citations

Trimerization of a yeast transcriptional activator via a coiled-coil motif

1989 • 392 citations

Stress-induced oligomerization and chromosomal relocalization of heat-shock factor

1991 • 391 citations

Induction of sequence-specific binding of Drosophila heat shock activator protein without protein synthesis

1987 • 382 citations

Cloning and characterization of two mouse heat shock factors with distinct inducible and constitutive DNA-binding ability.

1991 • 381 citations

Molecular cloning and expression of a hexameric Drosophila heat shock factor subject to negative regulation

1990 • 375 citations

DnaK, DnaJ, and GrpE heat shock proteins negatively regulate heat shock gene expression by controlling the synthesis and stability of sigma 32.

1990 • 356 citations

Isolation of a cDNA for HSF2: evidence for two heat shock factor genes in humans.

1991 • 347 citations

Heat shock gene regulation by nascent polypeptides and denatured proteins: hsp70 as a potential autoregulatory factor

1992 • 336 citations

The transport of proteins into the nucleus requires the 70-kilodalton heat shock protein or its cytosolic cognate.

1992 • 335 citations

Activating protein factor binds in vitro to upstream control sequences in heat shock gene chromatin

1984 • 329 citations

Three tomato genes code for heat stress transcription factors with a region of remarkable homology to the DNA-binding domain of the yeast HSF.

1990 • 321 citations

Physical interaction between heat shock proteins DnaK, DnaJ, and GrpE and the bacterial heat shock transcription factor σ32

1992 • 314 citations

Ultrastructural distribution of nuclear ribonucleoproteins as visualized by immunocytochemistry on thin sections.

1984 • 306 citations

In vitro activation of heat shock transcription factor DNA-binding by calcium and biochemical conditions that affect protein conformation.

1990 • 295 citations

Activation of Heat Shock Factor 2 during Hemin-Induced Differentiation of Human Erythroleukemia Cells

1992 • 281 citations

Activation in vitro of sequence-specific DNA binding by a human regulatory factor

1988 • 278 citations

1 The Stress Response, Function of the Proteins, and Perspectives

1990 • 271 citations

Yeast heat shock factor contains separable transient and sustained response transcriptional activators

1990 • 269 citations

Characterization of a novel chicken heat shock transcription factor, heat shock factor 3, suggests a new regulatory pathway.

1993 • 264 citations

Mammalian nuclei contain foci which are highly enriched in components of the pre-mRNA splicing machinery.

1991 • 243 citations

Heat-Inducible Human Factor That Binds to a Human hsp70 Promoter

1987 • 232 citations

Constitutive binding of yeast heat shock factor to DNA in vivo.

1988 • 221 citations

Immunoelectron microscopic localization of snRNPs

1984 • 208 citations

Escherichia coli heat shock gene mutants are defective in proteolysis.

1988 • 205 citations

Attenuation of the heat shock response in HeLa cells is mediated by the release of bound heat shock transcription factor and is modulated by changes in growth and in heat shock temperatures.

1991 • 197 citations

Molecular weight estimations of proteins by electrophoresis in polyacrylamide gels of graded porosity

1972 • 187 citations

The DnaK chaperone modulates the heat shock response of Escherichia coli by binding to the sigma 32 transcription factor.

1992 • 178 citations

Mutational analysis of the human HSP70 protein: distinct domains for nucleolar localization and adenosine triphosphate binding.

1989 • 175 citations

Modulation of stability of the Escherichia coli heat shock regulatory factor sigma

1989 • 169 citations

Heat shock-induced interactions of heat shock transcription factor and the human hsp70 promoter examined by in vivo footprinting.

1991 • 159 citations

A conserved heptapeptide restrains the activity of the yeast heat shock transcription factor.

1991 • 152 citations

Maximal stress-induced transcription from the human HSP70 promoter requires interactions with the basal promoter elements independent of rotational alignment.

1990 • 127 citations

Purified human factor activates heat shock promoter in a HeLa cell-free transcription system.

1988 • 106 citations

Hemin-induced transcriptional activation of the HSP70 gene during erythroid maturation in K562 cells is due to a heat shock factor-mediated stress response.

1989 • 97 citations

Retinoic acid receptor expression vector inhibits differentiation of F9 embryonal carcinoma cells.

1989 • 79 citations

Antibody-Mediated Activation of Drosophila Heat Shock Factor in Vitro

1990 • 70 citations

Alterations in chromatin conformation are accompanied by reorganization of nonchromatin domains that contain U-snRNP protein p28 and nuclear protein p107.

1985 • 40 citations

Macromolecular domains containing nuclear protein p107 and U-snRNP protein p28: Further evidence for an in situ nuclear matrix

1986 • 37 citations