The Saccharomyces cerevisiae CDC25 gene product binds specifically to catalytically inactive ras proteins in vivo.

Molecular Cloning: A Laboratory Manual

2001 • 133,517 citations

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

A novel genetic system to detect protein–protein interactions

1989 • 6,022 citations

The GTPase superfamily: conserved structure and molecular mechanism

1991 • 3,365 citations

[12] One-step gene disruption in yeast

1983 • 3,302 citations

Functional inactivation of genes by dominant negative mutations

1987 • 1,174 citations

cAMP-DEPENDENT PROTEIN KINASE: FRAMEWORK FOR A DIVERSE FAMILY OF REGULATORY ENZYMES

1990 • 1,121 citations

Refined crystal structure of the triphosphate conformation of H-ras p21 at 1.35 A resolution: implications for the mechanism of GTP hydrolysis.

1990 • 1,077 citations

Molecular Switch for Signal Transduction: Structural Differences Between Active and Inactive Forms of Protooncogenic ras Proteins

1990 • 1,037 citations

[12] High-efficiency transformation of yeast by electroporation

1991 • 798 citations

Inhibition of NIH 3T3 Cell Proliferation by a Mutant ras Protein with Preferential Affinity for GDP

1988 • 757 citations

The Saccharomyces cerevisiae Cell Cycle

1981 • 539 citations

Cloning and characterization of BCY1, a locus encoding a regulatory subunit of the cyclic AMP-dependent protein kinase in Saccharomyces cerevisiae.

1987 • 504 citations

The S. cerevisiae CDC25 gene product regulates the RAS/adenylate cyclase pathway

1987 • 491 citations

Three-dimensional structures of H-ras p21 mutants: Molecular basis for their inability to function as signal switch molecules

1990 • 395 citations

Copper activates metallothionein gene transcription by altering the conformation of a specific DNA binding protein

1988 • 365 citations

Tandem gene amplification mediates copper resistance in yeast.

1982 • 354 citations

S. cerevisiae genes IRA1 and IRA2 encode proteins that may be functionally equivalent to mammalian ras GTPase activating protein

1990 • 333 citations

Functional homology of mammalian and yeast RAS genes

1985 • 324 citations

ACE1 regulates expression of the Saccharomyces cerevisiae metallothionein gene.

1988 • 321 citations

IRA2, a second gene of Saccharomyces cerevisiae that encodes a protein with a domain homologous to mammalian ras GTPase-activating protein.

1990 • 305 citations

Mutant ras-encoded proteins with altered nucleotide binding exert dominant biological effects.

1986 • 285 citations

Copper metallothionein of yeast, structure of the gene, and regulation of expression.

1984 • 283 citations

SRV2, a gene required for RAS activation of adenylate cyclase in yeast

1990 • 263 citations

IRA1, an inhibitory regulator of the RAS-cyclic AMP pathway in Saccharomyces cerevisiae.

1989 • 251 citations

Cloning and characterization of CAP, the S. cerevisiae gene encoding the 70 kd adenylyl cyclase-associated protein

1990 • 249 citations

Characterization of Saccharomyces cerevisiae genes encoding subunits of cyclic AMP-dependent protein kinase.

1987 • 239 citations

CDC25 : a Component of the RAS -Adenylate Cyclase Pathway in Saccharomyces cerevisiae

1987 • 235 citations

The Function of Ras Genes in Saccharomyces Cerevisiae

1990 • 218 citations

Mammalian and Yeast ras Gene Products: Biological Function in Their Heterologous Systems

1985 • 209 citations

The CDC25 protein of Saccharomyces cerevisiae promotes exchange of guanine nucleotides bound to ras.

1991 • 196 citations

p36, the major cytoplasmic substrate of src tyrosine protein kinase, binds to its p11 regulatory subunit via a short amino-terminal amphiphatic helix.

1988 • 193 citations

The ras oncogene--an important regulatory element in lower eucaryotic organisms

1989 • 189 citations

Fermentable sugars and intracellular acidification as specific activators of the RAS‐adenylate cyclase signalling pathway in yeast: the relationship to nutrient‐induced cell cycle control

1991 • 173 citations

Dominant inhibitory Ras mutants selectively inhibit the activity of either cellular or oncogenic Ras.

1991 • 164 citations

Dominant yeast and mammalian RAS mutants that interfere with the CDC25-dependent activation of wild-type RAS in Saccharomyces cerevisiae.

1989 • 162 citations

CAP is a bifunctional component of the Saccharomyces cerevisiae adenylyl cyclase complex.

1991 • 155 citations

Isolation of ras GTP-binding mutants using an in situ colony-binding assay.

1986 • 153 citations

Enhancement of the GDP-GTP Exchange of RAS Proteins by the Carboxyl-Terminal Domain of SCD25

1990 • 152 citations

The CUP2 gene product regulates the expression of the CUP1 gene, coding for yeast metallothionein.

1989 • 134 citations

Crystal structure of an active form of RAS protein, a complex of a GTP analog and the HRAS p21 catalytic domain.

1990 • 112 citations

IRA2, an upstream negative regulator of RAS in yeast, is a RAS GTPase-activating protein.

1991 • 111 citations

Mutations of the Adenylyl Cyclase Gene that Block RAS Function in Saccharomyces cerevisiae

1990 • 109 citations

Heterologous Gene Expression inSaccharomyces cerevisiae

1985 • 109 citations

Identification of guanine nucleotides bound to ras-encoded proteins in growing yeast cells.

1987 • 98 citations

ACE2, an activator of yeast metallothionein expression which is homologous to SWI5.

1991 • 90 citations

Glucose‐induced cAMP signaling in Saccharomyces cerevisiae is mediated by the CDC25 protein

1989 • 87 citations

Regulatory function of the Saccharomyces cerevisiae RAS C-terminus.

1987 • 77 citations

SDC25, a CDC25-like gene which contains a RAS-activating domain and is a dispensable gene of Saccharomyces cerevisiae.

1991 • 76 citations

Studies of RAS Function in the Yeast Saccharomyces cerevisiae

1988 • 76 citations

The expression in yeast of the Escherichia coli galK gene on CYCI :: galK fusion plasmids

1983 • 74 citations

A CONVENIENT DOMINANT SELECTION MARKER FOR GENE TRANSFER IN INDUSTRIAL STRAINS OFSACCHAROMYCESYEAST:SMRIENCODED RESISTANCE TO THE HERBICIDE SULFOMETURON METHYL

1988 • 72 citations

A new RAS mutation that suppresses the CDC25 gene requirement for growth of Saccharomyces cerevisiae.

1988 • 52 citations

Mutants of H-ras that interfere with RAS effector function in Saccharomyces cerevisiae.

1989 • 51 citations

Copper and the ACE1 regulatory protein reversibly induce yeast metallothionein gene transcription in a mouse extract.

1989 • 50 citations

In vitro reconstitution of cdc25 regulated S. cerevisiae adenylyl cyclase and its kinetic properties.

1990 • 50 citations

Interactions between adenylate cyclase and the yeast GTPase-activating protein IRA1.

1991 • 48 citations

Different kinetic properties of the two mutants, RAS2Ile152 and RAS2Val19, that suppress the CDC25 requirement in RAS/adenylate cyclase pathway in Saccharomyces cerevisiae.

1990 • 41 citations

Complete nucleotide sequence of the gene encoding the regulatory subunit of 3′,5′-cyclic AMP-dependent protein kinase from the yeastSaccharomyces cerevisiae

1987 • 40 citations

Role of Heat Shock Transcription Factor in Yeast Metallothionein Gene Expression

1991 • 38 citations

IRA2, an upstream negative regulator of RAS in yeast, is a RAS GTPase protein

1991 • 35 citations

Domains of the Saccharomyces cerevisiae CDC25 gene controlling mitosis and meiosis

1988 • 32 citations