Changes in hormone responsiveness and cyclic AMP metabolism in rat hepatocytes during primary culture and effects of supplementing the medium with insulin and dexamethasone

PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT

1951 • 319,299 citations

Chapter 4 Preparation of Isolated Rat Liver Cells

1976 • 5,335 citations

HIGH-YIELD PREPARATION OF ISOLATED RAT LIVER PARENCHYMAL CELLS

1969 • 4,428 citations

A highly sensitive adenylate cyclase assay

1974 • 4,213 citations

Radioimmunoassay for Cyclic Nucleotides

1972 • 2,057 citations

Primary culture of parenchymal liver cells on collagen membranes

1975 • 808 citations

PARENCHYMAL CELLS FROM ADULT RAT LIVER IN NONPROLIFERATING MONOLAYER CULTURE

1973 • 427 citations

Hormonal stimulation of DNA synthesis in primary cultures of adult rat hepatocytes.

1976 • 359 citations

Studies on the alpha-adrenergic activation of hepatic glucose output. I. Studies on the alpha-adrenergic activation of phosphorylase and gluconeogenesis and inactivation of glycogen synthase in isolated rat liver parenchymal cells.

1976 • 350 citations

A simple ultrasensitive method for the assay of cyclic AMP and cyclic GMP in tissues

1976 • 328 citations

Primary monolayer cultures of adult rat liver parenchymal cells suitable for study of the regulation of enzyme synthesis

1974 • 321 citations

Control of hepatic glycogenolysis.

1980 • 288 citations

Interaction of Glucocorticoids with Glucagon and Epinephrine in the Control of Gluconeogenesis and Glycogenolysis in Liver and of Lipolysis in Adipose Tissue

1972 • 275 citations

Conditions affecting primary cell cultures of functional adult rat hepatocytes

1976 • 265 citations

Fetal phenotypic expression by adult rat hepatocytes on collagen gel/nylon meshes.

1979 • 251 citations

Stimulation of a low Km phosphodiesterase from liver by insulin and glucagon.

1978 • 208 citations

beta-adrenergic receptors in rat liver: effects of adrenalectomy.

1976 • 150 citations

Adrenergic control of glucose output and adenosine 3‘:5‘-monophosphate levels in hepatocytes from juvenile and adult rats.

1979 • 149 citations

[47] Liver cells

1979 • 120 citations

Independent Variation of Glucagon and Epinephrine Responsive Components of Hepatic Adenyl Cyclase as a Function of Age, Sex and Steroid Hormones

1970 • 119 citations

Induction of tryptophan oxygenase in primary rat liver cell suspensions by glucocorticoid hormone

1972 • 110 citations

Use of hepatocytes in primary culture for biochemical studies on liver functions

1982 • 102 citations

Adult liver parenchymal cells in primary culture: Characteristics and cell recognition standards

1974 • 102 citations

Purification and properties of the insulin-stimulated cyclic AMP phosphodiesterase from rat liver plasma membranes

1981 • 100 citations

Insulin exerts actions through a distinct species of guanine nucleotide regulatory protein: inhibition of adenylate cyclase

1983 • 97 citations

Hormonal activation of glycogen phosphorylase in hepatocytes from hypothyroid rats.

1978 • 86 citations

Development of cyclic AMP metabolism in rat liver a correlative study of tissue levels of cyclic AMP, accumulation of cyclic AMP in slices, adenylate cyclase activity and cyclic nucleotide phosphodiesterase activity

1973 • 84 citations

Hormonal Regulation of Phosphoenolpyruvate Carboxykinase in Primary Cultures of Adult‐Rat Liver Parenchymal Cells

1978 • 82 citations

Characterization of insulin-sensitive phosphodiesterase in fat cells. II. Comparison of enzyme activities stimulated by insulin and by isoproterenol.

1980 • 78 citations

Adenylate‐Cyclase Activity of Rat‐Liver Plasma Membranes

1973 • 76 citations

Improved methods for ATP analysis

1974 • 74 citations

AMINO ACID CONTROL OF PROTEIN SYNTHESIS AND DEGRADATION IN ISOLATED RAT HEPATOCYTES*

1980 • 73 citations

Conversion of adrenergic regulation of glycogen phosphorylase and synthase from an α to a β type during primary culture of rat hepatocytes

1982 • 71 citations

Chapter 31 Primary and Long-Term Culture of Adult Rat Liver Epithelial Cells

1976 • 67 citations

Convenient elimination of trichloroacetic acid prior to radioimmunoassay of cyclic nucleotides

1977 • 66 citations

Increased effect of adrenaline on cyclic AMP formation and positive β‐adrenergic modulation of DNA‐synthesis in regenerating hepatocytes

1980 • 57 citations

A Radioimmunoassay for cyclic AMP (cAMP) Obtained by Acetylation of Both Unlabeled and Labeled (³H‐cAMP) Ligand, or of Unlabeled Ligand only

1980 • 52 citations

Glucagon Binding and Adenylate Cyclase Activity in Liver Membranes from Untreated and Insulin-Treated Diabetic Rats

1978 • 49 citations

Glucagon control of cyclic AMP accumulation in isolated intact rat liver parenchymal cells in vitro

1974 • 44 citations

3':5'-cyclic AMP: independent induction of amino acid transport by epinephrine in primary cultures of adult rat liver cells.

1977 • 43 citations

Regulation of cyclic nucleotide phosphodiesterases in cultured hepatoma cells by dexamethasone and N6,O2'-dibutyryl adenosine 3':k'-monophosphate.

1977 • 42 citations

Rapid assay for cyclic AMP and cyclic GMP phosphodiesterases

1978 • 39 citations

Mechanisms for the emergence of catecholamine‐sensitive adenylate cyclase and β‐adrenergic receptors in cultured hepatocytes

1983 • 37 citations

Hormone-sensitive cAMP phosphodiesterase in liver and fat cells

1981 • 37 citations

Binding and action of insulin and glucagon in monolayer cultures and fresh suspensions of rat hepatocytes

1982 • 36 citations

An effect of glucagon on 3′,5′-cyclic AMP phosphodiesterase activity in isolated rat hepatocytes

1975 • 35 citations

Selective Effects of Insulin on Hepatic Epinephrine Responsive Adenyl Cyclase Activity

1972 • 35 citations

Direct Evidence of a Glucagon-Dependent Regulation of the Concentration of Glucagon Receptors in the Liver

1982 • 29 citations

Desensitization of Primary Cultures of Adult Rat Liver Parenchymal Cells to Stimulation of Adenosine 3′,5′-Monophosphate Production by Glucagon and Epinephrine*

1980 • 27 citations

Hormonally sensitive cyclic AMP phosphodiesterase in liver cells. An ecto-enzyme.

1981 • 25 citations

Enrichment of glucagon sensitive adenyl cyclase upon preparation of parenchymal cells from rat liver

1972 • 21 citations

Cyclic AMP metabolism in rat liver during 2-acetylaminofluorene carcinogenesis

1974 • 19 citations

Regulation of Target Cell Responsiveness

1984 • 17 citations

Regulation of Target Cell Responsiveness

1984 • 16 citations

The effect of streptozotocin-induced diabetes on the early steps of glucagon action in isolated rat liver cells

1980 • 15 citations

Adenylate cyclase and phosphodiesterase activities in rat hepatocytes cultured in the presence and absence of dexamethasone

1980 • 7 citations