Use of hepatocytes in primary culture for biochemical studies on liver functions

Chapter 4 Preparation of Isolated Rat Liver Cells

1976 • 5,335 citations

HIGH-YIELD PREPARATION OF ISOLATED RAT LIVER PARENCHYMAL CELLS

1969 • 4,428 citations

Primary culture of parenchymal liver cells on collagen membranes

1975 • 808 citations

PARENCHYMAL CELLS FROM ADULT RAT LIVER IN NONPROLIFERATING MONOLAYER CULTURE

1973 • 427 citations

Hormonal stimulation of DNA synthesis in primary cultures of adult rat hepatocytes.

1976 • 359 citations

Studies on the alpha-adrenergic activation of hepatic glucose output. I. Studies on the alpha-adrenergic activation of phosphorylase and gluconeogenesis and inactivation of glycogen synthase in isolated rat liver parenchymal cells.

1976 • 350 citations

Primary monolayer cultures of adult rat liver parenchymal cells suitable for study of the regulation of enzyme synthesis

1974 • 321 citations

Biochemical Studies on Liver Functions in Primary Cultured Hepatocytes of Adult Rats1

1978 • 318 citations

Studies on alpha-adrenergic activation of hepatic glucose output. Studies on role of calcium in alpha-adrenergic activation of phosphorylase.

1977 • 289 citations

Studies on alpha-adrenergic activation of hepatic glucose output. Relationship between alpha-adrenergic stimulation of calcium efflux and activation of phosphorylase in isolated rat liver parenchymal cells.

1978 • 259 citations

Use of Isolated Liver Cells and Kidney Tubules in Metabolic Studies

1976 • 252 citations

Fetal phenotypic expression by adult rat hepatocytes on collagen gel/nylon meshes.

1979 • 251 citations

Very low density lipoprotein synthesis and secretion by cultured rat hepatocytes.

1979 • 238 citations

Inhibition of protein degradation in isolated rat hepatocytes

1977 • 214 citations

Studies on the alpha-andrenergic activation of hepatic glucose output. II. Investigation of the roles of adenosine 3':5'-monophosphate and adenosine 3':5'-monophosphate-dependent protein kinase in the actions of phenylephrine in isolated hepatocytes.

1976 • 206 citations

Albumin, fibrinogen and transferrin synthesis in isolated rat hepatocyte suspensions. A model for the study of plasma protein synthesis

1975 • 202 citations

Respiratory Activity of Intact, Isolated Parenchymal Cells from Rat Liver

1968 • 199 citations

On the mechanism of ligand-induced down-regulation of insulin receptor level in the liver cell.

1981 • 198 citations

Effect of tunicamycin on the secretion of serum proteins by primary cultures of rat and chick hepatocytes. Studies on transferrin, very low density lipoprotein, and serum albumin

1978 • 195 citations

CELL TYPES IN LONG‐TERM PROPAGABLE CULTURES OF RAT LIVER*

1980 • 180 citations

Differentiation and Control of Malignancy of Tumor Cells

1976 • 180 citations

Down Regulation of Insulin Receptors in Primary Cultures of Adult Rat Hepatocytes in Monolayer*

1978 • 178 citations

Adenylate cyclase permanently uncoupled from hormone receptors in a novel variant of S49 mouse lymphoma cells.

1977 • 173 citations

Hormonal Control of Glycogenolysis and Gluconeogenesis in Isolated Rat Liver Cells

1973 • 158 citations

The effect of protease inhibitors and decreased temperature on the degradation of different classes of proteins in cultured hepatocytes

1979 • 157 citations

Regulation of malic enzyme synthesis by insulin triiodothyronine, and glucagon in liver cells in culture.

1976 • 155 citations

Activation of protein kinase and glycogen phosphorylase in isolated rat liver cells by glucagon and catecholamines.

1977 • 145 citations

Procarcinogen activation and hormonal control of cell proliferation in differentiated primary adult rat liver cell cultures

1977 • 143 citations

Growth state-dependent phenotypes of adult hepatocytes in primary monolayer culture.

1978 • 128 citations

Regulation of cholesterol esterification and biosynthesis in monolayer cultures of normal adult rat hepatocytes.

1980 • 127 citations

Plasma protein synthesis by isolated rat hepatocytes.

1977 • 119 citations

GROWTH CONTROL OF DIFFERENTIATED ADULT RAT HEPATOCYTES IN PRIMARY CULTURE*

1980 • 119 citations

Studies on the role of cyclic guanosine 3':5'-monophosphate and extracellular Ca2+ in the regulation of glycogenolysis in rat liver cells.

1976 • 119 citations

Demonstration of two classes of opiate binding sites in the nervous tissue of the marine mollusc Mytilus edulis. Positive homotropic cooperativity of lower affinity binding sites.

1980 • 117 citations

Unique distribution of glycoprotein receptors on parenchymal and sinusoidal cells of rat liver.

1979 • 111 citations

Hemoglobin-haptoglobin receptor in rat liver plasma membrane.

1980 • 108 citations

BIOCHEMICAL FUNCTIONS OF ADULT RAT HEPATOCYTES IN PRIMARY CULTURE*

1980 • 107 citations

Maintenance of microsomal cytochromes b5 and P-450 in primary cultures of parenchymal liver cells on collagen membranes

1976 • 95 citations

Permissive Effect of Dexamethasone on Glucagon Induction of Urea‐Cycle Enzymes in Perifused Primary Monolayer Cultures of Rat Hepatocytes

1979 • 95 citations

Liver cell adenylate cyclase and beta-adrenergic receptors. Increased beta-adrenergic receptor number and responsiveness in the hypothyroid rat.

1980 • 94 citations

Cytochrome P-450 Induction by Phenobarbital and 3-Methylcholanthrene in Primary Cultures of Hepatocytes

1976 • 93 citations

Effect of cobalt on synthesis of heme and cytochrome P-450 in the liver. Studies of adult rat hepatocytes in primary monolayer culture and in vivo.

1976 • 91 citations

Primary culture of normal adult rat liver cells which maintain stable urea cycle enzymes

1975 • 90 citations

Regulation of Lipogenesis and the Total Activities of Lipogenic Enzymes in a Primary Culture of Hepatocytes from Prenatal and Early Postnatal Chicks

1974 • 90 citations

Insulin and glucagon as a new regulator system for tryptophan oxygenase activity demonstrated in primary cultured rat hepatocytes.

1980 • 89 citations

Glycogenolytic response to glucagon of cultured fetal hepatocytes. Refractoriness following prior exposure to glucagon.

1975 • 86 citations

Induction of glucokinase by insulin under the permissive action of dexamethasone in primary rat hepatocyte cultures

1979 • 84 citations

Hormonal Regulation of Phosphoenolpyruvate Carboxykinase in Primary Cultures of Adult‐Rat Liver Parenchymal Cells

1978 • 82 citations

Hormonal regulation of glucokinase in primary cultures of adult rat hepatocytes.

1979 • 78 citations

Hormonal requirements for the induction of cytochrome P450 in hepatocytes cultured in a serum-free medium

1979 • 78 citations

Effect of insulin on ultrastructure and glycogenesis in primary cultures of adult rat hepatocytes

1977 • 77 citations

Characteristics of microsomal enzyme controls in primary cultures of rat hepatocytes

1979 • 76 citations

Effects of insulin on messenger RNA activities in rat liver.

1981 • 75 citations

Apparent maintenance of cytochrome P 450 by nicotinamide in primary cultures of rat hepatocytes

1979 • 75 citations

Thyroid thermogenesis in adult rat hepatocytes in primary monolayer culture: direct action of thyroid hormone in vitro.

1979 • 74 citations

Regulation of cholesterol synthesis in primary rat hepatocyte culture cells. Possible regulatory site at sterol demethylation.

1979 • 74 citations

AMINO ACID CONTROL OF PROTEIN SYNTHESIS AND DEGRADATION IN ISOLATED RAT HEPATOCYTES*

1980 • 73 citations

Intracellular localization and degradation of asialofetuin in isolated rat hepatocytes

1979 • 72 citations

Very low density lipoprotein secretion by cultured rat hepatocytes. Inhibition by albumin and other macromolecules.

1980 • 71 citations

Control mechanisms in cancer

1970 • 71 citations

Protein-catabolic state of isolated rat hepatocytes

1977 • 70 citations

Ascorbic acid deficiency and cytochrome P-450 in adult rat hepatocytes in primary monolayer culture

1979 • 70 citations

The effect of dexamethasone on formation of a fibronectin extracellular matrix by rat hepatocytes in vitro

1980 • 68 citations

Chapter 31 Primary and Long-Term Culture of Adult Rat Liver Epithelial Cells

1976 • 67 citations

Time-related changes in the synthesis and secretion of very low density, low density and high density lipoproteins by cultured rat hepatocytes

1981 • 65 citations

Extended expression of differentiated function in primary cultures of adult liver parenchymal cells maintained on nitrocellulose filters *1I. Induction of phosphoenolpyruvate carboxykinase and tyrosine aminotransferase

1978 • 64 citations

The activation of liver phosphorylase b kinase by glucagon

1976 • 61 citations

Coordinate regulation of acetyl coenzyme A carboxylase and fatty acid synthetase in liver cells of the developing chick in vivo and in culture

1978 • 60 citations

The Role of Growth Hormone, Dexamethasone and Triiodothyronine in the Regulation of Glutamine Synthetase in Primary Cultures of Rat Hepatocytes

1979 • 60 citations

Metabolic and enzymatic characteristics of adult rat liver parenchymal cells in non-proliferating primary monolayer cultures

1978 • 56 citations

Hormonal regulation of amino acid transport and gluconeogenesis in primary cultures of adult rat liver parenchymal cells

1976 • 55 citations

Production of apolipoproteins E and A-I by rat hepatocytes in primary culture

1980 • 55 citations

Plasminogen Is Synthesized by Primary Cultures of Rat Hepatocytes

1980 • 52 citations

Hormonal Regulation of Glucokinase in Primary Cultures of Rat Hepatocytes

1979 • 51 citations

Biochemical and medical aspects of tryptophan metabolism.

1980 • 50 citations

Role of insulin, glucose, and cyclic GMP in the regulation of glucokinase in cultured hepatocytes.

1981 • 50 citations

Role of cyclic AMP in the actions of catecholamines on hepatic carbohydrate metabolism.

1975 • 46 citations

Independent induction of tyrosine aminotransferase activity by dexamethasone and glucagon in isolated rat liver parenchymal cells in suspension and in monolayer culture in serum-free media

1980 • 42 citations

Synthesis by cultured hepatocytes of somatomedin and its binding protein

1979 • 42 citations

Influence of insulin, glucocorticoids and glucose on glycogen synthase activity in hepatocyte cultures

1980 • 41 citations

Inhibitor of protein degradation formed during incubation of isolated rat hepatocytes in a cell culture medium

1977 • 41 citations

Effects of hormones on the synthesis of α1 (acute-phase) glycoprotein in isolated rat hepatocytes

1977 • 38 citations

Two phase regulation of tyrosine aminotransferase activity by insulin in primary cultured hepatocytes of adult rats

1981 • 37 citations

Biosynthesis of transcobalamin II by adult rat liver parenchymal cells in culture

1976 • 35 citations

alpha 2 micro-Globulin in the rat. The regulation of the appearance of multiple forms in vivo and in primary cultures of adult hepatocytes.

1979 • 34 citations

Regulation of ATP-citrate lyase in primary cultures of adult rat hepatocytes.

1979 • 34 citations

Effects of leupeptin and pepstatin on protein turnover in adult rat hepatocytes in primary culture

1981 • 34 citations

Multiple Hormone Requirement for the Synthesis of α_2u- Globulin by Monolayers of Rat Hepatocytes in Long Term Primary Culture*

1980 • 33 citations

Effects of purine cyclic nucleotides on the growth of neonatal rat hepatocytes in primary tissue culture

1977 • 33 citations

Growth control in primary fetal rat liver cells in culture

1975 • 31 citations

The relationship between the ability of pyridine and substituted pyridines to maintain cytochrome P-450 and inhibit protein synthesis in rat hepatocyte cultures

1980 • 29 citations

The effect of ammonia, chloroquine, leupeptin, colchicine and cytochalasin B on degradation of high density lipoproteins in isolated rat hepatocytes

1980 • 29 citations

Tyrosine aminotransferase induction in hepatocytes cultured from rat foetuses treated with dexamethasone in utero

1979 • 28 citations

THE SIGNIFICANCE OF DIFFERENCES BETWEEN FRESH CELL SUSPENSIONS AND FRESH OR MAINTAINED MONOLAYERS*

1980 • 28 citations

Regulation of glucose-6-P dehydrogenase activity in primary rat hepatocyte cultures

1980 • 27 citations

Chapter 29 The Preparation and Characterization of Intact Isolated Parenchymal Cells from Rat Liver

1976 • 27 citations

Biochemical Studies on Liver Functions in Primary Cultured Hepatocytes of Adult Rats1: II. Regulation of Protein and Amino Acid Metabolism

1979 • 26 citations

Phosphoenolpyruvate Carboxykinase in Cultured Foetal Hepatocytes from the Rat

1980 • 24 citations

Role of the cytoskeleton in glycogenolysis stimulated by glucagon in primary cultures of adult rat hepatocytes

1980 • 22 citations

Maintenance of glucokinase activity in primary hepatocyte cultures

1980 • 22 citations

Precocious induction of glucokinase in primary cultures of postnatal rat hepatocytes

1979 • 21 citations

Hormonal regulation of serine dehydratase activity in primary cultures of adult rat hepatocytes

1981 • 21 citations

Factors regulating the induction of ornithine decarboxylase in fetal rat liver cells in culture

1978 • 20 citations

Hormonal control of ornithine decarboxylase in isolated liver cells and the effect of ethanol oxidation

1979 • 19 citations

ULTRASTRUCTURAL AND FUNCTIONAL STUDIES OF CULTURED ADULT RAT HEPATOCYTES

1980 • 19 citations

Induction of L-lysine-2-oxoglutarate reductase by glucagon and glucocorticoid in developing and adult rats in vivo and in vitro studies

1980 • 18 citations

Inhibition by cycloheximide of degradation of cytochrome P-450 in primary cultures of adult rat liver parenchymal cells and in vivo

1979 • 17 citations

Effects of Growth Hormone and Glucagon on Ornithine Decarboxylase Activity and Adenosine 3′,5′-Monophosphate Levels in Isolated Rat Hepatocytes*

1980 • 17 citations

Regulation of phosphoenolpyruvate carboxykinase by glucagon and glucocorticoids in primary cultures of rat hepatocytes

1980 • 15 citations

DNA synthesis and mitotic activity in short-term cultures of hepatocytes isolated from regenerating rat liver

1976 • 15 citations

Induction of hemoglobin-hydrolase activity by the thiol-protease inhibitors leupeptin and antipain in adult rat liver cells in primary culture

1979 • 15 citations

De novo synthesis of glucokinase in hepatocytes isolated from neonatal rats

1980 • 14 citations

Conversion of inactive 3‐hydroxy‐3‐methylglutaryl coenzyme a reductase to its active enzyme during culture of rat hepatocytes

1980 • 10 citations

STUDIES OF CREACTIVE PROTEIN SYNTHESIS BY PRIMARY CULTURES OF RABBIT HEPATOCYTES. *

1980 • 8 citations

The effect of cyclic AMP on glycoprotein secretion in isolated rat hepatocytes

1980 • 6 citations