Secreted proteases from pathogenic fungi

A new method for predicting signal sequence cleavage sites

1986 • 4,629 citations

BIOSYNTHETIC PROTEIN TRANSPORT AND SORTING BY THE ENDOPLASMIC RETICULUM AND GOLGI

1987 • 985 citations

Isolation of the putative structural gene for the lysine-arginine-cleaving endopeptidase required for processing of yeast prepro-α-factor

1984 • 698 citations

The Proteins Elafin, Filaggrin, Keratin Intermediate Filaments, Loricrin, and Small Proline-rich Proteins 1 and 2 Are Isodipeptide Cross-linked Components of the Human Epidermal Cornified Cell Envelope

1995 • 508 citations

PEP4 gene of Saccharomyces cerevisiae encodes proteinase A, a vacuolar enzyme required for processing of vacuolar precursors.

1986 • 400 citations

Expression of seven members of the gene family encoding secretory aspartyl proteinases in Candida albicans

1994 • 374 citations

Disruption of each of the secreted aspartyl proteinase genes SAP1, SAP2, and SAP3 of Candida albicans attenuates virulence

1997 • 297 citations

The PEP4 Gene Encodes an Aspartyl Protease Implicated in the Posttranslational Regulation of Saccharomyces cerevisiae Vacuolar Hydrolases

1986 • 292 citations

Candida albicans proteinases: resolving the mystery of a gene family

2001 • 283 citations

A triple deletion of the secreted aspartyl proteinase genes SAP4, SAP5, and SAP6 of Candida albicans causes attenuated virulence

1997 • 277 citations

Multiplicity of genes encoding secreted aspartic proteinases in Candida species

1994 • 251 citations

A comparison of secretory proteinases from different strains ofCandida albicans

1982 • 245 citations

Enzymatic cross-linking of involucrin and other proteins by keratinocyte particulates in vitro

1985 • 235 citations

Candidiasis

1997 • 233 citations

Expression of extracellular acid proteinase by proteolytic Candida spp. during experimental infection of oral mucosa

1988 • 228 citations

Secreted aspartic proteinase (Sap) activity contributes to tissue damage in a model of human oral candidosis

1999 • 226 citations

In Vitro and In Vivo Anticandidal Activity of Human Immunodeficiency Virus Protease Inhibitors

1999 • 215 citations

Differential expression of secreted aspartyl proteinases in a model of human oral candidosis and in patient samples from the oral cavity

1998 • 210 citations

Direct Evidence That Involucrin Is a Major Early Isopeptide Cross-linked Component of the Keratinocyte Cornified Cell Envelope

1997 • 200 citations

Purification and characterisation of an extracellular serine proteinase from Aspergillus fumigatus and its detection in tissue

1990 • 196 citations

In Vivo Analysis of Secreted Aspartyl Proteinase Expression in Human Oral Candidiasis

1999 • 194 citations

The PEP4 gene encodes an aspartyl protease implicated in the posttranslational regulation of Saccharomyces cerevisiae vacuolar hydrolases.

1986 • 192 citations

Evidence for possible involvement of an elastolytic serine protease in aspergillosis

1993 • 191 citations

Scanning electron microscopy of epidermal adherence and cavitation in murine candidiasis: a role for Candida acid proteinase

1988 • 189 citations

Widespread geographic distribution of oral Candida dubliniensis strains in human immunodeficiency virus-infected individuals

1997 • 187 citations

Pro‐sequence‐assisted protein folding

1995 • 184 citations

Evidence that Members of the Secretory Aspartyl Proteinase Gene Family, in ParticularSAP2,Are Virulence Factors forCandidaVaginitis

1999 • 173 citations

The expression of the secreted aspartyl proteinases Sap4 to Sap6 from Candida albicans in murine macrophages

1998 • 170 citations

Isolation and purification of an extracellular keratinase of Trichophyton mentagrophytes

1968 • 169 citations

Isolation and characterization of mutants of Aspergillus niger deficient in extracellular proteases

1992 • 169 citations

The Small Proline-Rich Proteins Constitute a Multigene Family of Differentially Regulated Cornified Cell Envelope Precursor Proteins

1995 • 162 citations

Isoation and characterisation of an extracellular alkaline protease of Aspergillus fumigatus

1991 • 156 citations

Purification and characterization of an elastinolytic metalloprotease from Aspergillus fumigatus and immunoelectron microscopic evidence of secretion of this enzyme by the fungus invading the murine lung

1994 • 156 citations

Evidence for degradation of gastrointestinal mucin by Candida albicans secretory aspartyl proteinase

1996 • 152 citations

Differential activation of a Candida albicans virulence gene family during infection

2000 • 150 citations

The alkaline protease of Aspergillus fumigatus is not a virulence determinant in two murine models of invasive pulmonary aspergillosis

1993 • 149 citations

Candida albicans ALS1: domains related to a Saccharomyces cerevisiae sexual agglutinin separated by a repeating motif

1995 • 149 citations

Virulence of alkaline protease-deficient mutants ofAspergillus fumigatus

1993 • 145 citations

An Aspergillus fumigatus alkaline protease mutant constructed by gene disruption is deficient in extracellular elastase activity

1992 • 144 citations

Isolation and Characterization of Proteinase from Candida albicans: Substrate Specificity

1984 • 143 citations

Risk Factors forCandida tropicalisFungemia in Patients with Cancer

2001 • 142 citations

Cloning and disruption of the gene encoding an extracellular metalloprotease of Aspergillus fumigatus

1994 • 141 citations

Isolation and characterization of a secreted metalloprotease of Aspergillus fumigatus

1993 • 137 citations

Effects of the Human Immunodeficiency Virus (HIV) Proteinase Inhibitors Saquinavir and Indinavir on In Vitro Activities of Secreted Aspartyl Proteinases of Candida albicans Isolates from HIV-Infected Patients

1999 • 132 citations

Differential regulation of SAP8 and SAPS, which encode two new members of the secreted aspartic proteinase family in Candida albicans

1998 • 131 citations

Secreted Aspartic Proteinase Family of Candida tropicalis

2001 • 129 citations

Biochemical and Antigenic Characterization of a New Dipeptidyl-Peptidase Isolated from Aspergillus fumigatus

1997 • 125 citations

Host‐induced, stage‐specific virulence gene activation in Candida albicans during infection

1999 • 122 citations

HIV-Protease Inhibitors Reduce Cell Adherence of Candida Albicans Strains by Inhibition of Yeast Secreted Aspartic Proteases

1999 • 119 citations

Nucleotide sequence of a genomic and a cDNA clone encoding an extracellular alkaline protease of Aspergillus fumigatus

1992 • 117 citations

Extracellular proteinase activity of Cryptococcus neoformans

1996 • 115 citations

KEX2 Influences Candida albicans Proteinase Secretion and Hyphal Formation

1997 • 114 citations

Degradation of humoral host defense by Candida albicans proteinase

1995 • 114 citations

Purification and characterization of a fibrinogenolytic serine proteinase from Aspergillus fumigatus culture filtrate

1992 • 112 citations

Isolation of an extracellular proteinase (Keratinase) fromMicrosporum canis

1982 • 109 citations

Single-strand conformation polymorphism analysis of PCR-amplified ribosomal DNA internal transcribed spacers to differentiate species of Aspergillus section Flavi

1996 • 108 citations

Mechanisms of adherence of Candida albicans to cultured human epidermal keratinocytes

1993 • 108 citations

Secreted Metalloprotease Gene Family of Microsporum canis

2002 • 107 citations

Germ Tubes and Proteinase Activity Contribute to Virulence of Candida albicans in Murine Peritonitis

1999 • 107 citations

Purification and characterization of major extracellular proteinases from Trichophyton rubrum

1985 • 105 citations

Role of aspartic proteases in disseminated Candida albicans infection in mice

1997 • 102 citations

Expression of Candida albicans SAP1 and SAP2 in experimental vaginitis

1995 • 101 citations

Isolation of a keratinolytic proteinase from Trichophyton mentagrophytes with enzymatic activity at acidic pH

1989 • 99 citations

Trichophyton Antigens Associated with IgE Antibodies and Delayed Type Hypersensitivity

1998 • 96 citations

Purification and characterization of a 315 kDa keratinolytic subtilisin-like serine protease from Microsporum canis and evidence of its secretion in naturally infected cats

1998 • 95 citations

A full length cDNA clone for the alkaline protease from Aspergillus oryzae: Structural analysis and expression in Saccharomyces cerevisiae

1989 • 91 citations

Isolation and nucleotide sequence of the extracellular acid protease gene (ACP) from the yeast Candida tropicalis

1991 • 90 citations

Purification and properties of the elastase from Aspergillus fumigatus

1992 • 89 citations

Dipeptidyl-peptidase IV secreted by Aspergillus fumigatus, a fungus pathogenic to humans

1997 • 89 citations

Purification and characterization of a 43·5 kDa keratinolytic metalloprotease fromMicrosporum canis

2001 • 88 citations

Characterization of a secretory proteinase of Candida parapsilosis and evidence for the absence of the enzyme during infection in vitro

1986 • 85 citations

Enzymic characteristics of secreted aspartic proteases of Candida albicans

2000 • 85 citations

Cleavage of immunoglobulins by pathogenic yeasts of the genus Candida.

1986 • 83 citations

Cloning and expression in yeast of a cDNA clone encoding Aspergillus oryzae neutral protease II, a unique metalloprotease

1991 • 76 citations

Isolation of a Microsporum canis Gene Family Encoding Three Subtilisin-Like Proteases Expressed in vivo

2002 • 76 citations

Purification and characterization of a 315 kDa keratinolytic subtilisin-like serine protease fromMicrosporum canisand evidence of its secretion in naturally infected cats

1998 • 73 citations

Virulence of an aspergillopepsin-deficient mutant ofAspergillus fumigatusand evidence for another aspartic proteinase linked to the fungal cell wall

1997 • 72 citations

Proteolytic Activation of the Interleukin-1β Precursor byCandida albicans

1998 • 72 citations

Fungal spores in lung and sputum

1978 • 71 citations

Activation of blood clotting factors by microbial proteinases

1994 • 70 citations

Purification and characterization of a 27,000-Mr extracellular proteinase from Trichophyton rubrum

1989 • 69 citations

Purification and partial characterization of an exocellular proteinase fromTrichophyton rubrum

1985 • 69 citations

Nucleotide sequence of a genomic and a cDNA clone encoding an extracellular alkaline protease ofAspergillus fumigatus

1992 • 69 citations

Proteolytic activity of a clinical isolate of Cryptococcus neoformans

1986 • 68 citations

Cloning and sequencing of two Candida parapsilosis genes encoding acid proteases

1993 • 67 citations

Extracellular proteolytic activity ofCryptococcus neoformans

1994 • 64 citations

Modulation of Experimental Systemic Murine Candidosis by Intravenous Pepstatin

1990 • 63 citations

Anti-fungal therapy at the HAART of viral therapy

2002 • 62 citations

Isolation and characterization of an elastinolytic proteinase from Aspergillus flavus

1990 • 62 citations

The gene and deduced protein sequences of the zymogen of Aspergillus niger acid proteinase A.

1991 • 62 citations

Definition of a Trichophyton protein associated with delayed hypersensitivity in humans. Evidence for immediate (IgE and IgG4) and delayed hypersensitivity to a single protein.

1996 • 59 citations

Molecular cloning and sequencing of the cDNA and gene for a novel elastinolytic metalloproteinase from Aspergillus fumigatus and its expression in Escherichia coli

1994 • 58 citations

Partial characterization of the extracellular keratinase fromMicrosporum canis

1984 • 57 citations

Cloning and characterisation ofpepC, a gene encoding a serine protease from Aspergillus niger

1993 • 55 citations

Comparative studies on keratinase production of Trichophyton mentagrophytes strains of animal origin

1995 • 54 citations

Degradation of human subendothelial extracellular matrix by proteinase-secreting Candida albicans

2006 • 52 citations

Acid Proteinase Secreted by Candida Tropicalis: Functional Analysis of Preproregion Cleavages in C. Tropicalis and Saccharomyces Cerevisiae

1996 • 52 citations

Exocellular proteolytic activity ofParacoccidioides brasiliensis: cleavage of components associated with the basement membrane

1998 • 51 citations

Purification and properties of leucine aminopeptidase IV from Aspergillus oryzae.

1977 • 50 citations

Molecular cloning and sequencing of the gene encoding an extracellular aspartic proteinase fromAspergillus fumigatus

1995 • 49 citations

Molecular cloning and targeted deletion of PEP2 which encodes a novel aspartic proteinase from Aspergillus fumigatus

2000 • 49 citations

Lack of vessel wall elastolysis in human invasive pulmonary aspergillosis

1992 • 47 citations

Molecular characterization and influence on fungal development of ALP2, a novel serine proteinase from Aspergillus fumigatus

2000 • 46 citations

Purification and Properties of Neutral Proteinase I from Aspergillus oryzae

1973 • 46 citations

Characterization of the Prolyl Dipeptidyl Peptidase Gene ( dppIV ) from the Koji Mold Aspergillus oryzae

1998 • 45 citations

Candidiasis visualised by proteinase-directed immunofluorescence

1991 • 45 citations

Characterization of an exocellular serine-thiol proteinase activity in Paracoccidioides brasiliensis

1995 • 44 citations

COS-1, a putative two-component histidine kinase ofCandida albicans, is anin vivovirulence factor

2001 • 43 citations

Adherence of different Candida dubliniensis isolates in the presence of fluconazole

2002 • 43 citations

Aspergillus species from hospital air and from patients

1990 • 43 citations

TheALS6 andALS7 genes ofCandida albicans

2000 • 41 citations

Purification, Characterization, and Heterologous Expression in Fusarium venenatum of a Novel Serine Carboxypeptidase from Aspergillus oryzae

1999 • 41 citations

On the renin-like activity of candida proteinases and activation of blood coagulation in vitro

1983 • 41 citations

Cloning and characterization of the pepE gene of Aspergillus niger encoding a new aspartic protease and regulation of pepE and pepC

1994 • 41 citations

Cloning, characterization and expression of pepF, a gene encoding a serine carboxypeptidase from Aspergillus niger

1994 • 41 citations

Cloning and characterization of the cDNAs and genes (mep20) encoding homologous metalloproteinases from Aspergillus flavus and A. fumigatus

1995 • 40 citations

Intra- and intermolecular events direct the propeptide-mediated maturation of the Candida albicans secreted aspartic proteinase Sap1p

2000 • 40 citations

A rapid method for detecting extracellular proteinase activity in Cryptococcus neoformans and a survey of 63 isolates

2000 • 40 citations

Purification and Properties of Neutral Proteinase II from Aspergillus oryzae

1973 • 39 citations

Aspergillosis of the Brain: Report of a Case

1958 • 39 citations

Purification and Properties of Leucine Aminopeptidase I from Aspergillus oryzae

1973 • 38 citations

The in vitro and in vivo Production of a 31.5-kD Keratinolytic Subtilase from <i>Microsporum canis</i> and the Clinical Status in Naturally Infected Cats

1998 • 35 citations

Candida albicans aspartic proteinase cleaves and inactivates human epidermal cysteine proteinase inhibitor, cystatin A

1994 • 33 citations

The Proteolytic Activity of the Recombinant Cryptic Human Fibronectin Type IV Collagenase from E. coli Expression

2000 • 33 citations

Purification and Properties of Alkaline Proteinase fromAspergillus oryzae

1973 • 32 citations

The Rhizopus oryzae secreted aspartic proteinase gene family: an analysis of gene expression

1998 • 32 citations

Degradation of human subendothelial extracellular matrix by proteinase-secreting Candida albicans

1997 • 31 citations

Molecular cloning of an extracellular aspartic proteinase fromRhizopus microsporusand evidence for its expression during infection

2002 • 30 citations

Purification and Properties of Acid Carboxypeptidase I fromAspergillus oryzae

1972 • 30 citations

Isolation, Purification and Characterization of Keratinolytic Proteinase fromMicrosporumcanis

1987 • 29 citations

Characterization of a Multicopy Family of Genes Encoding a Surface‐Expressed Serine Endoprotease in Rat Pneumocystis carinii

1999 • 26 citations

Purification and Properties of Leucine Aminopeptidase II from Aspergillus oryzae

1973 • 25 citations

Isolation and expression of a gene which encodes a wall-associated proteinase of Coccidioides immitis

1992 • 25 citations

Purification and Properties of Leucine Aminopeptidase III from Aspergillus oryzae

1973 • 23 citations

Characterization of a Vacuolar Protease in Neurospora crassa and the Use of Gene RIPing to Generate Protease-deficient Strains

1996 • 23 citations

Isolation and characterization of the Aspergillus oryzae gene encoding aspergillopepsin O

1993 • 22 citations

The effect of elastase-specific monoclonal and polyclonal antibodies on the virulence ofAspergillus fumigatus in immunocompromised mice

1994 • 21 citations

Purification and characterization of rhizopuspepsin isozymes from a liquid culture of rhizopus chinensis

1982 • 19 citations

Structure and Function of a Pepstatin-Insensitive Acid Proteinase from Aspergillus Niger var. Macrosporus

1991 • 19 citations

Purification and Properties of Acid Carboxypeptidase IV fromAspergillus oryzae

1973 • 18 citations

Cleavage of human big endothelin-1 byCandida albicansaspartic proteinase

1995 • 17 citations

Extracellular proteolytic enzyme activity ofHistoplasma capsulatum var.duboisii

1996 • 15 citations

The amino acid sequences of carboxypeptidases I and II from Aspergillus niger and their stability in the presence of divalent cations

1998 • 14 citations

The Proteolytic Activity and Cleavage Specificity of Fibronectin-Gelatinase and Fibronectin-Lamininase

1999 • 13 citations

Partial purification and kinetic studies of exocellular proteinase from Trichophyton mentagrophytes var. erinacei

1998 • 13 citations

Generation of non-bitter casein hydrolysates by using combinations of a proteinase and aminopeptidases

1999 • 12 citations

Purification and Properties of Acid Carboxypeptidase III fromAspergillus oryzae

1972 • 12 citations

Purification and Properties of Acid Carboxypeptidase II fromAspergillus oryzae

1972 • 9 citations

Production of proteinase and phospholipase by Paracoccidioides brasiliensis.

1999 • 7 citations

In vitro production of extracellular elastolytic proteinase by Histoplasma capsulatum var. duboisii and Histoplasma capsulatum var. capsulatum in the yeast phase

1991 • 7 citations

Beteiligung sekretorischer Candida‐Proteinasen an der Adhärenz von C.‐tropicalis‐Blastokonidien im Zellkultur‐Modell

1997 • 4 citations