Nef induces CD4 endocytosis: Requirement for a critical dileucine motif in the membrane-proximal CD4 cytoplasmic domain

Current protocols in molecular biology

1988 • 7,584 citations

Production of acquired immunodeficiency syndrome-associated retrovirus in human and nonhuman cells transfected with an infectious molecular clone

1986 • 2,918 citations

Human epidermal growth factor receptor cDNA sequence and aberrant expression of the amplified gene in A431 epidermoid carcinoma cells

1984 • 2,762 citations

The T4 gene encodes the AIDS virus receptor and is expressed in the immune system and the brain

1986 • 2,020 citations

Importance of the nef gene for maintenance of high virus loads and for development of AIDS

1991 • 1,613 citations

Genetic evidence for the involvement of the lck tyrosine kinase in signal transduction through the T cell antigen receptor

1992 • 1,046 citations

ZAP-70: A 70 kd protein-tyrosine kinase that associates with the TCR ζ chain

1992 • 1,037 citations

Safe and efficient generation of recombinant retroviruses with amphotropic and ecotropic host ranges.

1988 • 908 citations

Trans -Activator Gene of Human T-Lymphotropic Virus Type III (HTLV-III)

1985 • 872 citations

Interaction between CD4 and class II MHC molecules mediates cell adhesion

1987 • 823 citations

Signal-Dependent Membrane Protein Trafficking in the Endocytic Pathway

1993 • 808 citations

Serine phosphorylation-independent downregulation of cell-surface CD4 by nef

1991 • 760 citations

The CD4 receptor is complexed in detergent lysates to a protein-tyrosine kinase (pp58) from human T lymphocytes.

1988 • 716 citations

Signal transduction through the CD4 receptor involves the activation of the internal membrane tyrosine-protein kinase p56lck

1989 • 671 citations

The isolation and nucleotide sequence of a cDNA encoding the T cell surface protein T4: a new member of the immunoglobulin gene family

1985 • 669 citations

Direct visualization of binding, aggregation, and internalization of insulin and epidermal growth factor on living fibroblastic cells

1978 • 647 citations

Molecular Characterization of Human T-Cell Leukemia (Lymphotropic) Virus Type III in the Acquired Immune Deficiency Syndrome

1984 • 639 citations

Interaction of the unique N-terminal region of tyrosine kinase p56lck with cytoplasmic domains of CD4 and CD8 is mediated by cysteine motifs

1990 • 629 citations

Visualization by fluorescence of the binding and internalization of epidermal growth factor in human carcinoma cells A-431.

1978 • 578 citations

Temporal aspects of DNA and RNA synthesis during human immunodeficiency virus infection: evidence for differential gene expression

1989 • 574 citations

Human immunodeficiency virus type 1 Vpu protein induces rapid degradation of CD4

1992 • 553 citations

T cell antigen receptor signal transduction: A tale of tails and cytoplasmic protein-tyrosine kinases

1993 • 552 citations

A novel di-leucine motif and a tyrosine-based motif independently mediate lysosomal targeting and endocytosis of CD3 chains

1992 • 540 citations

HIV F/3' orf encodes a phosphorylated GTP-binding protein resembling an oncogene product

1987 • 523 citations

The CD4 and CD8 antigens are coupled to a protein-tyrosine kinase (p56lck) that phosphorylates the CD3 complex.

1989 • 447 citations

Requirement for association of p56lck with CD4 in antigen-specific signal transduction in T cells

1991 • 417 citations

Expression Cloning of Human EGF Receptor Complementary DNA: Gene Amplification and Three Related Messenger RNA Products in A431 Cells

1984 • 406 citations

Molecular Biology and Function of CD4 and CD8

1989 • 388 citations

Cells nonproductively infected with HIV-1 exhibit an aberrant pattern of viral RNA expression: A molecular model for latency

1990 • 371 citations

Nef Protein of HIV-1 Is a Transcriptional Repressor of HIV-1 LTR

1988 • 366 citations

The Ick tyrosine protein kinase interacts with the cytoplasmic tail of the CD4 glycoprotein through its unique amino-terminal domain

1989 • 362 citations

Down regulation of epidermal growth factor receptors: direct demonstration of receptor degradation in human fibroblasts.

1984 • 357 citations

Mutational analysis of the human immunodeficiency virus: the orf-B region down-regulates virus replication.

1987 • 310 citations

Molecular Events Mediating T Cell Activation

1990 • 303 citations

Downregulation of cell-surface CD4 expression by simian immunodeficiency virus Nef prevents viral super infection.

1993 • 291 citations

Altered T cell activation and development in transgenic mice expressing the HIV-1 nef gene.

1993 • 282 citations

Experimentally induced alteration in the polarity of developing neurons

1987 • 274 citations

Lack of a negative influence on viral growth by the nef gene of human immunodeficiency virus type 1.

1989 • 266 citations

Human immunodeficiency virus type 1 negative factor is a transcriptional silencer.

1989 • 243 citations

Demonstration of epidermal growth factor-induced receptor dimerization in living cells using a chemical covalent cross-linking agent.

1988 • 228 citations

CD4 is retained in the endoplasmic reticulum by the human immunodeficiency virus type 1 glycoprotein precursor

1990 • 228 citations

Nef protein of human immunodeficiency virus type 1: evidence against its role as a transcriptional inhibitor.

1989 • 227 citations

Envelope glycoprotein of HIV induces interference and cytolysis resistance in CD4+ cells: Mechanism for persistence in AIDS

1988 • 223 citations

A new HTLV-III/LAV Encoded Antigen Detected by Antibodies from AIDS Patients

1985 • 212 citations

Expression and function of CD4 in a murine T-cell hybridoma

1987 • 208 citations

Human immunodeficiency virus as a prototypic complex retrovirus

1991 • 197 citations

Expression of the type 1 human immunodeficiency virus Nef protein in T cells prevents antigen receptor-mediated induction of interleukin 2 mRNA.

1991 • 192 citations

Rapid phosphorylation and modulation of the T4 antigen on cloned helper T cells induced by phorbol myristate acetate or antigen.

1986 • 171 citations

Natural HIV-1 NEF accelerates virus replication in primary human lymphocytes

1992 • 158 citations

Association of tyrosine kinase p56lck with CD4 inhibits the induction of growth through the αβ T-cell receptor

1992 • 146 citations

Phorbol ester-induced downregulation of CD4 is a multistep process involving dissociation from p56lck, increased association with clathrin-coated pits, and altered endosomal sorting.

1993 • 144 citations

Efficient replication of human immunodeficiency virus type 1 requires a threshold level of Rev: potential implications for latency

1992 • 144 citations

The protein tyrosine kinase p56lck inhibits CD4 endocytosis by preventing entry of CD4 into coated pits

1992 • 141 citations

Modulation of CD4 by antigenic activation.

1987 • 138 citations

Transient modulation and internalization of T4 antigen induced by phorbol esters.

1986 • 137 citations

Differential endocytosis of CD4 in lymphocytic and nonlymphocytic cells.

1991 • 137 citations

Cytoplasmic localization of the HTLV-III 3′orf protein in cultured T cells

1986 • 134 citations

Intracellular interaction of human immunodeficiency virus type 1 (ARV-2) envelope glycoprotein gp160 with CD4 blocks the movement and maturation of CD4 to the plasma membrane

1990 • 131 citations

A human cell factor is essential for HIV-1 Rev action.

1990 • 120 citations

Phosphorylation-dependent down-modulation of CD4 requires a specific structure within the cytoplasmic domain of CD4

1991 • 120 citations

Nef from primary isolates of human immunodeficiency virus type 1 suppresses surface CD4 expression in human and mouse T cells

1993 • 118 citations

The negative effect of human immunodeficiency virus type 1 Nef on cell surface CD4 expression is not species specific and requires the cytoplasmic domain of CD4

1993 • 114 citations

Structural features of the cytoplasmic region of CD4 required for internalization.

1990 • 111 citations

Internalization and recycling of CD4 transfected into HeLa and NIH3T3 cells.

1989 • 102 citations

Vpu-induced degradation of CD4: requirement for specific amino acid residues in the cytoplasmic domain of CD4

1993 • 91 citations

Phosphorylation of CD4 and CD8 molecules following T cell triggering.

1987 • 87 citations

The roles of CD4 and CD8 in T cell activation.

1991 • 86 citations

CD4: Collaborator in immune recognition and HIV infection

1990 • 86 citations

T4 endocytosis and phosphorylation induced by phorbol esters but not by mitogen or HIV infection.

1988 • 81 citations

Human immunodeficiency virus type 1 Vpu protein induces degradation of chimeric envelope glycoproteins bearing the cytoplasmic and anchor domains of CD4: role of the cytoplasmic domain in Vpu-induced degradation in the endoplasmic reticulum

1993 • 81 citations

Allelic variation in the effects of the nef gene on replication of human immunodeficiency virus type 1.

1991 • 73 citations

Reduced cell surface expression of processed human immunodeficiency virus type 1 envelope glycoprotein in the presence of Nef

1993 • 66 citations

Disruption of the CD4-p56lck complex is required for rapid internalization of CD4.

1992 • 62 citations

Human immunodeficiency virus type 1 glycoprotein precursor retains a CD4-p56lck complex in the endoplasmic reticulum

1992 • 61 citations

High‐yield trapping of EGF‐induced receptor dimers by chemical cross‐linking ¹

1989 • 55 citations

Mutational analysis of the human immunodeficiency virus type 1 Eli Nef function.

1992 • 49 citations

Regulation of CD4 and CD8 surface expression on human thymocyte subpopulations by triggering through CD2 and the CD3-T cell receptor.

1989 • 34 citations

Degradation of CD4 following phorbol-induced internalization in human T lymphocytes. Evidence for distinct endocytic routing of CD4 and CD3.

1992 • 30 citations

CD4 and CD8 act as co-receptors during thymic selection of the T cell repertoire.

1991 • 18 citations

Autonomous roles for the cytoplasmic domains of the CD2 and CD4 T cell surface antigens.

1991 • 18 citations

The cytoplasmic tail of CD4 targets chimeric molecules to a degradative pathway.

1991 • 4 citations