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Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase

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Antibodies, a laboratory manual

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A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.

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Genomic sequencing.

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[1] Getting started with yeast

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Guide to Yeast Genetics and Molecular Biology

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High efficiency transformation of intact yeast cells using single stranded nucleic acids as a carrier

1989 • 2,140 citations

Methods in Yeast Genetics: A Laboratory Course Manual

1990 • 1,922 citations

A protein assembly-disassembly pathway in vitro that may correspond to sequential steps of synaptic vesicle docking, activation, and fusion

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Identification of 23 complementation groups required for post-translational events in the yeast secretory pathway

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Multifunctional yeast high-copy-number shuttle vectors

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Two differentially regulated mRNAs with different 5′ ends encode secreted and intracellular forms of yeast invertase

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Methods in Yeast Genetics — A Laboratory Course Manual

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Site-directed mutagenesis of virtually any plasmid by eliminating a unique site

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[21] β-Galactosidase gene fusions for analyzing gene expression in Escherichia coli and yeast

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A simple and efficient procedure for transformation of yeasts.

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Guide to Yeast Genetics and Molecular Biology

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Implications of the SNARE hypothesis for intracellular membrane topology and dynamics

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Early stages in the yeast secretory pathway are required for transport of carboxypeptidase Y to the vacuole

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SNAPs, a family of NSF attachment proteins involved in intracellular membrane fusion in animals and yeast

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A fusion protein required for vesicle-mediated transport in both mammalian cells and yeast

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A rab protein is required for the assembly of SNARE complexes in the docking of transport vesicles

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Different base/base mismatches are corrected with different efficiencies by the methyl-directed DNA mismatch-repair system of E. coli

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Compartmental organization of Golgi-specific protein modification and vacuolar protein sorting events defined in a yeast sec18 (NSF) mutant.

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GTPases: Multifunctional Molecular Switches Regulating Vesicular Traffic

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Yeast syntaxins Sso1p and Sso2p belong to a family of related membrane proteins that function in vesicular transport.

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n-Sec1: a neural-specific syntaxin-bindingprotein.

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An NSF-like ATPase, p97, and NSF mediate cisternal regrowth from mitotic golgi fragments

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Identification and structure of four yeast genes (SLY) that are able to suppress the functional loss of YPT1, a member of the RAS superfamily.

1991 • 347 citations

Distinct biochemical requirements for the budding, targeting, and fusion of ER-derived transport vesicles.

1991 • 318 citations

Homologs of the synaptobrevin/VAMP family of synaptic vesicle proteins function on the late secretory pathway in S. cerevisiae

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SED5 encodes a 39-kD integral membrane protein required for vesicular transport between the ER and the Golgi complex.

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A multisubunit particle implicated in membrane fusion.

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Dissection of a single round of vesicular transport: Sequential intermediates for intercisternal movement in the Golgi stack

1989 • 269 citations

Vesicle fusion following receptor-mediated endocytosis requires a protein active in Golgi transport

1989 • 250 citations

The GTP-binding protein Ypt1 is required for transport in vitro: the Golgi apparatus is defective in ypt1 mutants.

1989 • 234 citations

A novel 115-kD peripheral membrane protein is required for intercisternal transport in the Golgi stack.

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Characterization of a component of the yeast secretion machinery: identification of the SEC18 gene product.

1988 • 220 citations

GDI1 encodes a GDP dissociation inhibitor that plays an essential role in the yeast secretory pathway.

1994 • 200 citations

Ypt1p implicated in v-SNARE activation

1994 • 199 citations

p115 is a general vesicular transport factor related to the yeast endoplasmic reticulum to Golgi transport factor Uso1p.

1995 • 199 citations

The yeast SLY gene products, suppressors of defects in the essential GTP-binding Ypt1 protein, may act in endoplasmic reticulum-to-Golgi transport.

1991 • 193 citations

Binding of an N-ethylmaleimide-sensitive fusion protein to Golgi membranes requires both a soluble protein(s) and an integral membrane receptor.

1989 • 184 citations

BET1, BOS1, and SEC22 Are Members of a Group of Interacting Yeast Genes Required for Transport from the Endoplasmic Reticulum to the Golgi Complex

1990 • 176 citations

A cytoskeleton-related gene, uso1, is required for intracellular protein transport in Saccharomyces cerevisiae.

1991 • 164 citations

The yeast SEC17 gene product is functionally equivalent to mammalian alpha-SNAP protein.

1992 • 159 citations

Purification of three related peripheral membrane proteins needed for vesicular transport.

1990 • 147 citations

Sec12p-dependent membrane binding of the small GTP-binding protein Sar1p promotes formation of transport vesicles from the ER.

1991 • 143 citations

Bos1p, an integral membrane protein of the endoplasmic reticulum to Golgi transport vesicles, is required for their fusion competence

1993 • 137 citations

Genetic interactions between KAR2 and SEC63, encoding eukaryotic homologues of DnaK and DnaJ in the endoplasmic reticulum.

1993 • 135 citations

Transcytosis-associated protein (TAP)/p115 is a general fusion factor required for binding of vesicles to acceptor membranes.

1995 • 114 citations

BET1, BOS1, and SEC22 are members of a group of interacting yeast genes required for transport from the endoplasmic reticulum to the Golgi complex.

1990 • 104 citations

ADP-ribosylation factor and coatomer couple fusion to vesicle budding

1994 • 104 citations

Identification and Structure of Four Yeast Genes (SLY) That Are Able To Suppress the Functional Loss of YPT1, a Member of the RAS Superfamily

1991 • 97 citations

Biochemical requirements for the targeting and fusion of ER-derived transport vesicles with purified yeast Golgi membranes.

1996 • 92 citations

Characterization of a Component of the Yeast Secretion Machinery: Identification of the SEC18 Gene Product

1988 • 79 citations

Control of protein traffic between distinct plasma membrane domains. Requirement for a novel 108,000 protein in the fusion of transcytotic vesicles with the apical plasma membrane.

1993 • 71 citations

The Yeast SLY Gene Products, Suppressors of Defects in the Essential GTP-Binding Ypt1 Protein, May Act in Endoplasmic Reticulum-to-Golgi Transport

1991 • 65 citations

Functionality and specific membrane localization of transport GTPases carrying C-terminal membrane anchors of synaptobrevin-like proteins.

1995 • 44 citations

GTPases: Multifunctional Molecular Switches Regulating Vesicular Traffic

1994 • 20 citations

Molecular Characterization of the USO1 Gene Product which Is Essential for Vesicular Transport in Saccharomyces cerevisiae

1994 • 16 citations