A highly active decarboxylating dehydrogenase with rationally inverted coenzyme specificity.

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

[19] Rapid and efficient site-specific mutagenesis without phenotypic selection

1987 • 5,413 citations

Redesign of the coenzyme specificity of a dehydrogenase by protein engineering

1990 • 727 citations

Inactivation of isocitrate dehydrogenase by phosphorylation is mediated by the negative charge of the phosphate.

1987 • 300 citations

A Specific, Highly Active Malate Dehydrogenase by Redesign of a Lactate Dehydrogenase Framework

1988 • 276 citations

Regulation of an Enzyme by Phosphorylation at the Active Site

1990 • 246 citations

Catalytic mechanism of NADP+-dependent isocitrate dehydrogenase: implications from the structures of magnesium-isocitrate and NADP+ complexes

1991 • 244 citations

Purification and properties of phosphorylated isocitrate dehydrogenase of Escherichia coli.

1979 • 160 citations

Electrostatic and Steric Contributions to Regulation at the Active Site of Isocitrate Dehydrogenase

1990 • 118 citations

Creation of an NADP-dependent pyruvate dehydrogenase multienzyme complex by protein engineering

1993 • 113 citations

Structure of isocitrate dehydrogenase with isocitrate, nicotinamide adenine dinucleotide phosphate, and calcium at 2.5-.ANG. resolution: A pseudo-Michaelis ternary complex

1993 • 108 citations

Structure of 3–isopropylmalate dehydrogenase in complex with NAD+: ligand–induced loop closing and mechanism for cofactor specificity

1994 • 82 citations

Kinetic mechanism of Escherichia coli isocitrate dehydrogenase

1993 • 77 citations

Alteration of coenzyme specificity of malate dehydrogenase from Thermus flavus by site-directed mutagenesis.

1993 • 71 citations

Genes encoding two isocitrate dehydrogenase isozymes of a psychrophilic bacterium, Vibrio sp. strain ABE-1

1993 • 68 citations

Kinetic analysis of NAD+-isocitrate dehydrogenase with altered isocitrate binding sites: Contribution of IDH1 and IDH2 subunits to regulation and catalysis

1993 • 62 citations

The importance of arginine 102 for the substrate specificity of Escherichia coli malate dehydrogenase

1992 • 46 citations

Tartrate Dehydrogenase, a New Member of the Family of Metal-Dependent Decarboxylating R-Hydroxyacid Dehydrogenases

1994 • 40 citations

Yeast diphosphopyridine nucleotide specific isocitrate dehydrogenase. Binding of ligands

1971 • 39 citations

Leucine biosynthesis in Saccharomyces cerevisiae. Purification and characterization of beta-isopropylmalate dehydrogenase.

1980 • 31 citations

Co-enzyme specificity of 3-isopropylmalate dehydrogenase from Thermits thermophilus HB8

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Importance of lysine-286 at the NADP site of glutamate dehydrogenase from Salmonella typhimurium

1992 • 13 citations