Gene expression profiling: monitoring transcription and translation products using DNA microarrays and proteomics

Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

1979 • 54,907 citations

The Hallmarks of Cancer

2000 • 27,569 citations

High resolution two-dimensional electrophoresis of proteins.

1975 • 19,515 citations

Cluster analysis and display of genome-wide expression patterns

1998 • 16,410 citations

Molecular Classification of Cancer: Class Discovery and Class Prediction by Gene Expression Monitoring

1999 • 11,586 citations

Distinct types of diffuse large B-cell lymphoma identified by gene expression profiling

2000 • 9,712 citations

Quantitative Monitoring of Gene Expression Patterns with a Complementary DNA Microarray

1995 • 9,575 citations

Cluster Analysis

1974 • 9,459 citations

p53, the Cellular Gatekeeper for Growth and Division

1997 • 7,621 citations

Electrospray Ionization for Mass Spectrometry of Large Biomolecules

1989 • 7,324 citations

Laser desorption ionization of proteins with molecular masses exceeding 10,000 daltons

1988 • 5,560 citations

Differential Display of Eukaryotic Messenger RNA by Means of the Polymerase Chain Reaction

1992 • 5,105 citations

Lessons from Hereditary Colorectal Cancer

1996 • 4,900 citations

Broad patterns of gene expression revealed by clustering analysis of tumor and normal colon tissues probed by oligonucleotide arrays

1999 • 4,195 citations

Correlation between Protein and mRNA Abundance in Yeast

1999 • 3,800 citations

Serial Analysis of Gene Expression

1995 • 3,659 citations

High resolution two-dimensional electrophoresis of basic as well as acidic proteins

1977 • 3,556 citations

“Field cancerization” in oral stratified squamous epithelium. Clinical implications of multicentric origin

1953 • 3,421 citations

Interpreting patterns of gene expression with self-organizing maps: Methods and application to hematopoietic differentiation

1999 • 2,990 citations

Expression monitoring by hybridization to high-density oligonucleotide arrays

1996 • 2,796 citations

A model for p53-induced apoptosis

1997 • 2,502 citations

Two‐dimensional electrophoresis. The current state of two‐dimensional electrophoresis with immobilized pH gradients

1988 • 2,431 citations

Knowledge-based analysis of microarray gene expression data by using support vector machines

2000 • 2,328 citations

Large-Scale Identification, Mapping, and Genotyping of Single-Nucleotide Polymorphisms in the Human Genome

1998 • 2,163 citations

Systematic variation in gene expression patterns in human cancer cell lines

2000 • 2,119 citations

Identification of genes differentially regulated by interferon α, β, or γ using oligonucleotide arrays

1998 • 1,781 citations

Accessing Genetic Information with High-Density DNA Arrays

1996 • 1,511 citations

The current state of two-dimensional electrophoresis with immobilized pH gradients

2000 • 1,498 citations

A gene expression database for the molecular pharmacology of cancer

2000 • 1,447 citations

Distinctive gene expression patterns in human mammary epithelial cells and breast cancers

1999 • 1,410 citations

Light-generated oligonucleotide arrays for rapid DNA sequence analysis.

1994 • 1,353 citations

A comparison of selected mRNA and protein abundances in human liver

1997 • 1,274 citations

Progress with Proteome Projects: Why all Proteins Expressed by a Genome Should be Identified and How To Do It

1996 • 1,252 citations

Protein mapping by combined isoelectric focusing and electrophoresis of mouse tissues

1975 • 1,213 citations

Gene Expression Profiles in Normal and Cancer Cells

1997 • 1,157 citations

Proteome and proteomics: New technologies, new concepts, and new words

1998 • 1,036 citations

Isoelectric focusing in immobilized pH gradients: Principle, methodology and some applications

1982 • 963 citations

Laser Capture Microdissection: Molecular Analysis of Tissue

1997 • 890 citations

Proteomics: quantitative and physical mapping of cellular proteins

1999 • 878 citations

From Proteins to Proteomes: Large Scale Protein Identification by Two-Dimensional Electrophoresis and Arnino Acid Analysis

1996 • 837 citations

A Physical Map of 30,000 Human Genes

1998 • 723 citations

Investigation of variant Creutzfeldt-Jakob disease and other human prion diseases with tonsil biopsy samples

1999 • 710 citations

Gene expression profiles of laser-captured adjacent neuronal subtypes

1999 • 708 citations

The dynamic range of protein expression: A challenge for proteomic research

2000 • 599 citations

Analysis of gene expression data using self‐organizing maps

1999 • 558 citations

Oncoprotein Networks

1997 • 532 citations

Reference points for comparisons of two‐dimensional maps of proteins from different human cell types defined in a pH scale where isoelectric points correlate with polypeptide compositions

1994 • 523 citations

Improvement of the solubilization of proteins in two‐dimensional electrophoresis with immobilized pH gradients

1997 • 484 citations

Cellular gene expression altered by human cytomegalovirus: Global monitoring with oligonucleotide arrays

1998 • 445 citations

Detection of 14‐3‐3 protein in the cerebrospinal fluid supports the diagnosis of Creutzfeldt‐Jakob disease

1998 • 439 citations

Global analysis of gene expression in pulmonary fibrosis reveals distinct programs regulating lung inflammation and fibrosis

2000 • 415 citations

In vivo gene expression profile analysis of human breast cancer progression.

1999 • 396 citations

Using oligonucleotide probe arrays to access genetic diversity

1999 • 345 citations

New zwitterionic detergents improve the analysis of membrane proteins by two‐dimensional electrophoresis

1998 • 334 citations

A thousand points of light: The application of fluorescence detection technologies to two-dimensional gel electrophoresis and proteomics

2000 • 326 citations

Protein identification methods in proteomics

2000 • 324 citations

cDNA microarrays detect activation of a myogenic transcription program by thePAX3-FKHRfusion oncogene

1999 • 313 citations

High-level expression of Egr-1 and Egr-1–inducible genes in mouse and human atherosclerosis

2000 • 293 citations

New variant Creutzfeldt-Jakob disease: neurological features and diagnostic tests

1997 • 287 citations

Versatile derivatisation of solid support media for covalent bonding on DNA-microchips

1999 • 274 citations

DNA chips: promising toys have become powerful tools

1999 • 265 citations

Proteome profiling of bladder squamous cell carcinomas: identification of markers that define their degree of differentiation.

1997 • 205 citations

Twenty years of two‐dimensional electrophoresis: Past, present and future

1996 • 193 citations

2D protein electrophoresis: can it be perfected?

1999 • 182 citations

Protein-electroblotting and -microsequencing strategies in generating protein data bases from two-dimensional gels.

1989 • 180 citations

Molecular Cloning and Expression of the Transformation Sensitive Epithelial Marker Stratifin

1993 • 177 citations

Abnormal Proteins in the Cerebrospinal Fluid of Patients with Creutzfeldt–Jakob Disease

1986 • 171 citations

14-3-3 Protein, Neuron-Specific Enolase, and S-100 Protein in Cerebrospinal Fluid of Patients with Creutzfeldt-Jakob Disease

1998 • 171 citations

Biosensor chip mass spectrometry: A chip-based proteomics approach

2000 • 171 citations

Architecture and polypeptide composition of HeLa cytoskeletons

1982 • 165 citations

A high-density probe array sample preparation method using 10- to 100-fold fewer cells

1999 • 160 citations

From genes to protein structure and function: novel applications of computational approaches in the genomic era

2000 • 160 citations

Frequency and specificity of antiheart antibodies in patients with dilated cardiomyopathy detected using SDS-PAGE and western blotting

1993 • 158 citations

Two-dimensional gel electrophoresis of proteins : methods and applications

1984 • 156 citations

Human and mouse proteomic databases: novel resources in the protein universe

1998 • 155 citations

Applications of SYPRO Orange and SYPRO Red Protein Gel Stains

1996 • 152 citations

Proliferation-related expression of p19/nm23 nucleoside diphosphate kinase.

1992 • 149 citations

Bladder Squamous Cell Carcinomas Express Psoriasin and Externalize it to the Urine

1996 • 146 citations

Loss of adipocyte-type fatty acid binding protein and other protein biomarkers is associated with progression of human bladder transitional cell carcinomas.

1996 • 146 citations

Bovine dilated cardiomyopathy: Proteomic analysis of an animal model of human dilated cardiomyopathy

1999 • 139 citations

Subproteomics based upon protein cellular location and relative solubilities in conjunction with composite two-dimensional electrophoresis gels

2000 • 133 citations

Up-dated catalogue of HeLa cell proteins: percentages and characteristics of the major cell polypeptides labeled with a mixture of 16 14C-labeled amino acids.

1982 • 132 citations

The human keratinocyte two‐dimensional gel protein database (update 1992): Towards an integrated approach to the study of cell proliferation, differentiation and skin diseases

1992 • 125 citations

Development of a Toxicological Gene Array and Quantitative Assessment of This Technology

2000 • 125 citations

The human keratinocyte two‐dimensional gel protein database (update 1995): Mapping components of signal transduction pathways

1995 • 115 citations

Genes upregulated in the human trabecular meshwork in response to elevated intraocular pressure.

2000 • 113 citations

Cardiac protein abnormalities in dilated cardiomyopathy detected by two‐dimensional polyacrylamide gel electrophoresis

1998 • 112 citations

Up-dated catalogue of HeLa cell proteins: percentages and characteristics of the major cell polypeptides labeled with a mixture of 16 14C-labeled amino acids.

1982 • 109 citations

Better approaches to finding the needle in a haystack: Optimizing proteome analysis through automation

2000 • 109 citations

ANTIBODY ENGINEERING

1997 • 105 citations

New insights into cyclosporine A nephrotoxicity by proteome analysis

1998 • 103 citations

The analysis of S100A9 and S100A8 expression in matched sets of macroscopically normal colon mucosa and colorectal carcinoma: The S100A9 and S100A8 positive cells underlie and invade tumor mass

1999 • 99 citations

Short-term culturing of low-grade superficial bladder transitional cell carcinomas leads to changes in the expression levels of several proteins involved in key cellular activities

1999 • 98 citations

Proteomics and immunohistochemistry define some of the steps involved in the squamous differentiation of the bladder transitional epithelium: a novel strategy for identifying metaplastic lesions.

1999 • 97 citations

Psoriasin (S100A7): A putative urinary marker for the follow-up of patients with bladder squamous cell carcinomas

1999 • 96 citations

Analysis of polypeptide expression in benign and malignant human breast lesions: down-regulation of cytokeratins

1996 • 92 citations

Identification of transformation sensitive proteins recorded in human two‐dimensional gel protein databases by mass spectrometric peptide mapping alone and in combination with microsequencing

1994 • 87 citations

Changes in myocardial protein expression in pacing-induced canine heart failure

1999 • 85 citations

Expression of tropomyosin isoforms in benign and malignant human breast lesions

1996 • 85 citations

Construction of HSC‐2DPAGE: A two‐dimensional gel electrophoresis database of heart proteins

1997 • 81 citations

Protein abundance alterations in matched sets of macroscopically normal colon mucosa and colorectal carcinoma

1999 • 80 citations

Human proteins sensitive to neoplastic transformation in cultured epithelial and fibroblast cells.

1982 • 74 citations

Gene expression in normal and virally transformed mouse 3T3B and hamster BHK21 cells

1980 • 70 citations

Human cellular protein patterns and their link to genome DNA sequence data: usefulness of two‐dimensional gel electrophoresis and microsequencing

1991 • 67 citations

A qualitative and quantitative protein database approach identifies individual and groups of functionally related proteins that are differentially regulated in simian virus 40 (SV40) transformed human keratinocytes: An overview of the functional changes associated with the transformed phenotype

1994 • 63 citations

Autoantibodies in Sera of Patients with Myocarditis: Characterization of the Corresponding Proteins by Isoelectric Focusing and N-Terminal Sequence Analysis

1997 • 63 citations

Studying heart disease using the proteomic approach

2000 • 62 citations

An integrated proteomic approach to studying glomerular nephrotoxicity

1999 • 61 citations

Identification of mitochondrial antigens recognized by antibodies in sera of patients with idiopathic dilated cardiomyopathy by two-dimensional gel electrophoresis and protein sequencing

1997 • 61 citations

An integrated proteomic approach to studying glomerular nephrotoxicity

1999 • 59 citations

A comprehensive protein ressource for the study of bladder cancer: http://biobase.dk/cgi-bin/celis

1999 • 59 citations

Rapid identification of proteins.

1993 • 57 citations

High-throughput gene expression analysis for drug discovery

2000 • 52 citations

Quantitative analysis of Op18 phosphorylation in childhood acute leukemia

1997 • 48 citations

Human proteins sensitive to neoplastic transformation in cultured epithelial and fibroblast cells.

1982 • 47 citations

A two-dimensional electrophoresis database of rat heart proteins

1999 • 45 citations

DNA arrays: technology, options and toxicological applications

2000 • 44 citations

Protein-Electroblotting and Microsequencing in Establishing Integrated Human Protein Databases

1991 • 43 citations

Assessment of homogeneity in polypeptide expression in breast carcinomas shows widely variable expression in highly malignant tumors

1996 • 41 citations

Proteomic analysis of simulated occupational jet fuel exposure in the lung

1999 • 36 citations

Recombinogenic targeting: a new approach to genomic analysis—a review

1998 • 35 citations

Cell cycle progression is associated with distinct patterns of phosphorylation of Op18

1992 • 35 citations

Inappropriate ubiquitin conjugation: a proposed mechanism contributing to heart failure

1997 • 31 citations

Diffusion-weighted MRI in familial Creutzfeldt–Jakob disease with the codon 200 mutation in the prion protein gene

2005 • 30 citations

The yeast two-hybrid screening technique and its use in the study of protein-protein interactions in apoptosis

1998 • 29 citations

Gene expression profiles of the collecting duct in the mouse renal inner medulla

2000 • 29 citations

Two‐dimensional electrophoretic analysis of transformation‐sensitive polypeptides during chemically, spontaneously, and oncogene‐induced transformation of rat liver epithelial cells

1992 • 27 citations

Functional genomics and DNA array techniques in atherosclerosis research

1999 • 27 citations

Overexpression of calcium-binding protein calgranulin B in colonic mucosal diseases

1997 • 25 citations

Analysis of oligodendroglial differentiation using cDNA arrays

2000 • 24 citations

Mapping in the Sequencing Era

1999 • 10 citations

Measuring Scales for Pocket Spectroscopes

1878 • 9 citations

Expression of cDNA clones by coupled in vitro transcription/translation and transfection into COS‐1 cells: Protein mapping in two‐dimensional gels

1995 • 8 citations

Celera in talks to launch private sector human proteome project

2000 • 7 citations

A Manual of Determinative Bacteriology . By F. D. Chester, Delaware College Agricultural Experiment Station. N. Y., The Macmillan Co. Price, $2.60.

1901 • 3 citations

Deleted Work

1955 • 0 citations