Progress with Proteome Projects: Why all Proteins Expressed by a Genome Should be Identified and How To Do It

High resolution two-dimensional electrophoresis of proteins.

1975 • 19,515 citations

A Film Detection Method for Tritium‐Labelled Proteins and Nucleic Acids in Polyacrylamide Gels

1974 • 8,693 citations

Differential Display of Eukaryotic Messenger RNA by Means of the Polymerase Chain Reaction

1992 • 5,105 citations

High resolution two-dimensional electrophoresis of basic as well as acidic proteins

1977 • 3,556 citations

Two‐dimensional electrophoresis. The current state of two‐dimensional electrophoresis with immobilized pH gradients

1988 • 2,431 citations

The physical map of the whole E. coli chromosome: Application of a new strategy for rapid analysis and sorting of a large genomic library

1987 • 1,768 citations

Rapid identification of proteins by peptide-mass fingerprinting

1993 • 1,619 citations

Error-Tolerant Identification of Peptides in Sequence Databases by Peptide Sequence Tags

1994 • 1,475 citations

Identifying proteins from two-dimensional gels by molecular mass searching of peptide fragments in protein sequence databases.

1993 • 1,301 citations

In-gel digestion of proteins for internal sequence analysis after one- or two-dimensional gel electrophoresis

1992 • 1,259 citations

Nucleotide sequence of bacteriophage λ DNA

1982 • 1,171 citations

Isoelectric focusing in immobilized pH gradients: Principle, methodology and some applications

1982 • 963 citations

Use of mass spectrometric molecular weight information to identify proteins in sequence databases

1993 • 936 citations

Progress with gene‐product mapping of the Mollicutes: Mycoplasma genitalium

1995 • 934 citations

The complete DNA sequence of yeast chromosome III

1992 • 851 citations

From Proteins to Proteomes: Large Scale Protein Identification by Two-Dimensional Electrophoresis and Arnino Acid Analysis

1996 • 837 citations

Improvement of an "In-Gel" Digestion Procedure for the Micropreparation of Internal Protein Fragments for Amino Acid Sequencing

1995 • 755 citations

Improved Resolution and Very High Sensitivity in MALDI TOF of Matrix Surfaces Made by Fast Evaporation

1994 • 682 citations

Peptide Mass Maps: A Highly Informative Approach to Protein Identification

1993 • 678 citations

Protein Identification by Mass Profile Fingerprinting

1993 • 595 citations

A new generation of information retrieval tools for biologists: the example of the ExPASy WWW server

1994 • 564 citations

Autoradiography using storage phosphor technology

1990 • 531 citations

Identification of differentially expressed mRNA species by an improved disply technique (DDRT-PCR)

1993 • 523 citations

Gene number, noise reduction and biological complexity

1995 • 462 citations

Staining of the Ca2+-binding proteins, calsequestrin, calmodulin, troponin C, and S-100, with the cationic carbocyanine dye “Stains-all”.

1983 • 422 citations

Two-dimensional gel analysis of soluble proteins. Charaterization of guinea pig exocrine pancreatic proteins.

1975 • 394 citations

A nonlinear wide‐range immobilized pH gradient for two‐dimensional electrophoresis and its definition in a relevant pH scale

1993 • 386 citations

Immobilized pH Gradients: Theory and Methodology

1990 • 363 citations

The ENZYME data bank.

1994 • 345 citations

The QUEST System for Quantitative Analysis of Two-dimensional Gels

1989 • 308 citations

Development of polyacrylamide gels that improve the separation of proteins and their detection by silver staining

1988 • 305 citations

Selective identification and differentiation of N‐and O‐linked oligosaccharides in glycoproteins by liquid chromatography‐mass spectrometry

1993 • 285 citations

A comparison between low background silver diammine and silver nitrate protein stains

1992 • 266 citations

Quantitative analysis of protein synthesis in mouse embryos. I. Extensive reprogramming at the one- and two-cell stages

1991 • 234 citations

Matrix-assisted laser desorption ionization mass spectrometry of proteins electroblotted after polyacrylamide gel electrophoresis

1994 • 223 citations

Micropreparative two‐dimensional electrophoresis allowing the separation of samples containing milligram amounts of proteins

1993 • 191 citations

Gene‐Protein database of Escherichia coli K ‐ 12: Edition 3

1990 • 167 citations

An updated two‐dimensional gel database of rat liver proteins useful in gene regulation and drug effect studies

1995 • 163 citations

Site-specific carbohydrate identification in recombinant proteins using MALD-TOF MS

1993 • 162 citations

Identification of proteins in polyacrylamide gels by mass spectrometric peptide mapping combined with database search

1994 • 161 citations

Comprehensive two‐dimensional gel protein databases offer a global approach to the analysis of human cells: The transformed amnion cells (AMA) master database and its link to genome DNA sequence data

1990 • 158 citations

Protein identification by peptide mass fingerprinting.

1994 • 157 citations

The MELANIE project: From a biopsy to automatic protein map interpretation by computer

1991 • 149 citations

Sugar chains of serum transferrin from patients with carbohydrate deficient glycoprotein syndrome. Evidence of asparagine-N-linked oligosaccharide transfer deficiency.

1993 • 147 citations

Improved mass accuracy in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry of peptides

1994 • 144 citations

A two‐dimensional gel database of human plasma proteins

1991 • 143 citations

The gene‐protein database of Escherichia coli: Edition 5

1992 • 142 citations

Protein fingerprinting by SDS-gel electrophoresis after partial fragmentation with CNBr

1979 • 133 citations

Identification of mouse brain proteins after two‐dimensional electrophoresis and electroblotting by microsequence analysis and amino acid composition analysis

1988 • 132 citations

The REF52 Protein Database

1989 • 131 citations

Human liver protein map: A reference database established by microsequencing and gel comparison

1992 • 129 citations

From Electrophoretically Separated Protein to Identification: Strategies for Sequence and Mass Analysis

1994 • 124 citations

Plasma and red blood cell protein maps: Update 1993

1993 • 122 citations

Mass spectrometric analysis of blotted proteins after gel electrophoretic separation by matrix‐assisted laser desorption/ionization

1992 • 120 citations

SMALL‐SCALE FRACTIONATION OF PROTEINS AND NUCLEIC ACIDS BY ISOELECTRIC FOCUSING IN POLYACRYLAMIDE GELS*

1973 • 111 citations

SWISS‐2DPAGE: A database of two‐dimensional gel electrophoresis images

1993 • 110 citations

Identification of myocardial proteins from two‐dimensional gels by peptide mass fingerprinting

1995 • 109 citations

Peptide ladder sequencing by mass spectrometry using a novel, volatile degradation reagent

1994 • 107 citations

Imidazole‐SDS‐Zn reverse staining of proteins in gels containing or not SDS and microsequence of individual unmodified electroblotted proteins

1992 • 107 citations

Glycosylation sites identified by solid-phase Edman degradation: O-linked glycosylation motifs on human glycophorin A

1993 • 104 citations

Protein composition of the human heart: The construction of a myocardial two‐dimensional electrophoresis database

1994 • 103 citations

Comparative two-dimensional gel analysis and microsequencing identifies gelsolin as one of the most prominent downregulated markers of transformed human fibroblast and epithelial cells.

1990 • 99 citations

Analytical and micropreparative peptide mapping by high performance liquid chromatography/electrospray mass spectrometry of proteins purified by gel electrophoresis

1993 • 96 citations

Electrospray Ionization Mass Spectrometry on Hydrophobic Peptides Electroeluted from Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis Application to the Topology of the Sarcoplasmic Reticulum Ca2+ ATPase

1993 • 91 citations

Protein identifications for aSaccharomyces cerevisiae protein database

1994 • 87 citations

Identification of transformation sensitive proteins recorded in human two‐dimensional gel protein databases by mass spectrometric peptide mapping alone and in combination with microsequencing

1994 • 87 citations

Elsie 4: Quantitative computer analysis of sets of two-dimensional gel electrophoretograms

1988 • 85 citations

Amino Acid Analysis and Protein Database Compositional Search as a Rapid and Inexpensive Method to Identify Proteins

1994 • 85 citations

Monosaccharide and oligosaccharide analysis of proteins transferred to polyvinylidene fluoride membranes after sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

1993 • 80 citations

Global approaches to quantitative analysis of gene-expression patterns observed by use of two-dimensional gel electrophoresis.

1984 • 80 citations

Amino acid analysis on polyvinylidene difluoride membranes

1989 • 77 citations

In situ chemical cleavage of proteins immobilized to glass-fiber and polyvinylidenedifluoride membranes: Cleavage at tryptophan residues with 2-(2′-nitrophenylsulfenyl)-3-methyl-3′-bromoindolenine to obtain internal amino acid sequence

1990 • 74 citations

Quantitation of human leukocyte proteins after silver staining: A study with two‐dimensional electrophoresis

1991 • 71 citations

Covalent protein modifications and gene expression changes in rodent liver following administration of methapyrilene: A study using two-dimensional electrophoresis

1992 • 71 citations

Preparative isoelectric focusing in immobilized pH gradients. I. General principles and methodology

1983 • 70 citations

Global changes in gene expression related to antibiotic synthesis in Streptomyces hygroscopicus

1992 • 70 citations

The human myocardial two‐dimensional gel protein database: Update 1994

1994 • 67 citations

Two‐dimensional electrophoretic analysis of proteins expressed by normal and cancerous human crypts: Application of mass spectrometry to peptide‐mass fingerprinting

1994 • 65 citations

Effects of Chronic Ethanol on Enzymes Regulating Sialylation and Desialylation of Transferrin in Rats

1993 • 65 citations

A qualitative and quantitative protein database approach identifies individual and groups of functionally related proteins that are differentially regulated in simian virus 40 (SV40) transformed human keratinocytes: An overview of the functional changes associated with the transformed phenotype

1994 • 63 citations

A human myocardial two‐dimensional electrophoresis database: Protein characterisation by microsequencing and immunoblotting

1992 • 62 citations

Mineral-binding proteoglycans of fetal porcine calvarial bone.

1988 • 61 citations

'Catalysts' for polyacrylamide gel polymerization and detection of proteins by silver staining.

1988 • 60 citations

Two-Dimensional Polyacrylamide Gel Electrophoresis of Proteins Labeled with the Fluorophore Monobromobimane Prior to First-Dimensional Isoelectric Focusing: Imaging of the Fluorescent Protein Spot Patterns Using a Cooled Charge-Coupled Device

1993 • 59 citations

Improved high-performance liquid chromatography of amino acids derivatised with 9-fluorenylmethyl chloroformate

1996 • 59 citations

Rapid identification of proteins.

1993 • 57 citations

Improving the detection of proteins after transfer to polyvinylidene difluoride membranes

1992 • 57 citations

A two‐dimensional gel protein database of noncultured total normal human epidermal keratinocytes: Identification of proteins strongly up‐regulated in psoriatic epidermis

1990 • 56 citations

Interferon‐γ up‐regulates a unique set of proteins in human keratinocytes

1993 • 55 citations

Microsequences of 145 proteins recorded in the two‐dimensional gel protein database of normal human epidermal keratinocytes

1992 • 54 citations

Isoelectric focusing in immobilized pH gradients in the pH 10–11 range

1987 • 51 citations

Mouse liver protein database: A catalog of proteins detected by two‐dimensional gel electrophoresis

1992 • 50 citations

Identification of tissue proteins by amino acid analysis after purification by two-dimensional electrophoresis

1992 • 49 citations

Quantitative exploration of the REF52 protein database: Cluster analysis reveals the major protein expression profiles in responses to growth regulation, serum stimulation, and viral transformation

1990 • 49 citations

Structural characterization of blotting membranes and the influence of membrane parameters for electroblotting and subsequent amino acid sequence analysis of proteins

1993 • 48 citations

Database and search techniques for two‐dimensional gel protein data: A comparison of paradigms for exploratory data analysis and prospects for biological modeling

1989 • 43 citations

Analysis of embryonic mouse development: construction of a high-resolution, two-dimensional gel protein database.

1992 • 39 citations

Analysis of Glycoproteins Separated by Two-Dimensional Gel Electrophoresis Using Lectin Blotting Revealed by Chemiluminescence

1994 • 38 citations

Genomically linked cellular protein databases derived from two‐dimensional polyacrylamide gel electrophoresis

1989 • 38 citations

Preparation of colloidal gold for staining proteins electrotransferred onto nitrocellulose membranes

1988 • 37 citations

Evaluation of storage phosphor imaging for quantitative analysis of 2-D gels using the Quest II system.

1993 • 36 citations

India ink staining of proteins on nylon and hydrophobic membranes

1988 • 36 citations

Quantification of proteins in the subnanogram and nanogram range: Comparison of the AuroDye, FerriDye, and India ink staining methods

1989 • 33 citations

Protein blotting on nitrocellulose: some important aspects of the resolution and detection of antigens in complex extracts

1987 • 33 citations

Characterization of Human Plasma Glycoproteins Separated by Two-Dimensional Gel Electrophoresis

1996 • 31 citations

Identification of proteins in sequence databases from amino acid composition data

1991 • 30 citations

Purification and identification of tyrosine‐phosphorylated proteins from B lymphocytes stimulated through the antigen receptor

1994 • 30 citations

Measurement of picomoles of phosphoamino acids by high-performance liquid chromatography

1991 • 29 citations

Complexity of sea urchin embryo nuclear proteins that contain basic domains.

1992 • 28 citations

The rat liver epithelial (RLE) cell protein database

1991 • 27 citations

Workshop on two‐dimensional gel protein databases

1992 • 26 citations

Identification of macrophage activation associated proteins by two-dimensional gel electrophoresis and microsequencing

1995 • 26 citations

Peptide mapping and microsequencing of proteins separated by SDS-PAGE after limited in situ acid hydrolysis.

1992 • 25 citations

Sequence analysis of peptide mixtures by automated integration of Edman and mass spectrometric data

1992 • 23 citations

The SWISS-2DPAGE database of two-dimensional polyacrylamide gel electrophoresis.

1994 • 22 citations

Size polymorphisms due to changes in the number of O-glycosylated tandem repeats in the Dictyostelium discoideum glycoprotein PsA.

1992 • 22 citations

Dose-responses in rat hepatic protein modification and expression following exposure to the rat hepatocarcinogen methapyrilene

1994 • 21 citations

A fast spot segmentation algorithm for two‐dimensional gel electrophoresis analysis

1993 • 18 citations

Towards stoichiometric silver staining of proteins resolved in complex two‐dimensional electrophoresis gels: Real‐time analysis of pattern development

1993 • 17 citations

Applications of an automated apparatus for two‐dimensional electrophoresis, Model TEP‐1, for microsequence analyses of proteins

1992 • 15 citations

A distributed system for two-dimensional gel analysis

1994 • 15 citations

The rat liver epithelial (RLE) cell nuclear protein database

1993 • 14 citations

Two-dimensional maps in very acidic immobilized pH gradients

1990 • 14 citations

A clinical molecular scanner: the Melanie project.

1991 • 13 citations

REF52 on global gel navigator: An internet‐accessible two‐dimensional gel electrophoresis database

1994 • 13 citations

Amino Acid Analysis of PVDF Bound Proteins

1992 • 12 citations

The amino acid composition of 350 lymphocyte proteins

1994 • 12 citations

Glycan microheterogeneity of α1-antitrypsin in serum and meconium from normal and cystic fibrosis patients by crossed immunoaffinoelectrophoresis with different lectins (Con A, LCA, WGA)

1993 • 10 citations

An efficient disk based data structure for rapid searching of quantitative two‐dimensional gel databases

1993 • 10 citations

Ultramicrodetection of proteins in polyacrylamide gels

1992 • 9 citations

Mechanical precision in two-dimensional electrophoresis can improve protein spot positional reproducibility.

1993 • 9 citations

[29] Two-dimensional gel analysis of protein synthesis

1993 • 7 citations

Beyond silver staining

1992 • 7 citations

Comparison of the Bio Image Visage 2000 and the GELLAB-II two-dimensional electrophoresis image analysis systems.

1993 • 6 citations

Acid phosphatase typing for breeding nematode‐resistant tomatoes by isoelectric focusing with an ultranarrow immobilized pH gradient

1988 • 5 citations

The influence of the support material used on band sharpness in Immobiline gels

1988 • 5 citations