The current state of two-dimensional electrophoresis with immobilized pH gradients

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

High resolution two-dimensional electrophoresis of proteins.

1975 • 19,515 citations

Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels

1987 • 4,186 citations

High resolution two-dimensional electrophoresis of basic as well as acidic proteins

1977 • 3,556 citations

A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels

1980 • 3,334 citations

Ultrasensitive Stain for Proteins in Polyacrylamide Gels Shows Regional Variation in Cerebrospinal Fluid Proteins

1981 • 2,972 citations

Two‐dimensional electrophoresis. The current state of two‐dimensional electrophoresis with immobilized pH gradients

1988 • 2,431 citations

Femtomole sequencing of proteins from polyacrylamide gels by nano-electrospray mass spectrometry

1996 • 1,687 citations

Cell Biology: a Laboratory Handbook

1995 • 1,226 citations

Protein mapping by combined isoelectric focusing and electrophoresis of mouse tissues

1975 • 1,213 citations

Complete Sequence Analysis of the Genome of the Bacterium Mycoplasma Pneumoniae

1996 • 1,143 citations

Technical improvements in two‐dimensional electrophoresis increase the level of genetic variation detected in wheat‐seedling proteins

1986 • 801 citations

Improved silver staining procedure for fast staining in PhastSystem Development Unit. I. Staining of sodium dodecyl sulfate gels

1988 • 697 citations

Proteome Research: New Frontiers in Functional Genomics

1997 • 484 citations

Sample application by in‐gel rehydration improves the resolution of two‐dimensional electrophoresis with immobilized pH gradients in the first dimension

1994 • 367 citations

Immobilized pH Gradients: Theory and Methodology

1990 • 363 citations

Use of thiourea to increase the solubility of membrane proteins in two‐dimensional electrophoresis

1998 • 342 citations

Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K‐12

1997 • 341 citations

Solubilization of proteins for electrophoretic analyses

1996 • 316 citations

Improved and simplified in‐gel sample application using reswelling of dry immobilized pH gradients

1997 • 309 citations

Towards higher resolution: Two-dimensional Electrophoresis ofSaccharomyces cerevisiae proteins using overlapping narrow immobilized pH gradients

2000 • 276 citations

Elimination of point streaking on silver stained two‐dimensional gels by addition of iodoacetamide to the equilibration buffer

1987 • 271 citations

Two‐dimensional polyacrylamide gel electrophoresis with immobilized pH gradients in the first dimensin (IPG‐Dalt): The state of the art and the controversy of vertical versus horizontal systems

1995 • 266 citations

Advances in protein solubilisation for two-dimensional electrophoresis

1999 • 256 citations

Recent developments in two-dimensional gel electrophoresis with immobilized pH gradients: Wide pH gradients up to pH 12, longer separation distances and simplified procedures

1999 • 251 citations

Electrophoretic characterization of wheat grain allergens from different cultivars involved in bakers' asthma

1993 • 244 citations

Improved protein solubility in two‐dimensional electrophoresis using tributyl phosphine as reducing agent

1998 • 241 citations

Very alkaline immobilized pH gradients for two‐dimensional electrophoresis of ribosomal and nuclear proteins

1997 • 238 citations

Proteome Research: Two-Dimensional Gel Electrophoresis and Identification Methods

2000 • 237 citations

Proteomics and automation

1999 • 209 citations

Comparison of yeast cell protein solubilization procedures for two-dimensional electrophoresis

1999 • 202 citations

Gel electrophoresis: Proteins

1994 • 201 citations

Micropreparative two‐dimensional electrophoresis allowing the separation of samples containing milligram amounts of proteins

1993 • 191 citations

Two-dimensional electrophoresis

1991 • 185 citations

Gel gradient electrophoresis, isoelectric focusing and two-dimensional techniques in horizontal, ultrathin polycrylamide layers

1980 • 174 citations

Interlaboratory reproducibility of yeast protein patterns analyzed by immobilized pH gradient two‐dimensional gel electrophoresis

1995 • 152 citations

Positional reproducibility of protein spots in two‐dimensional polyacrylamide gel electrophoresis using immobilised pH gradient isoelectric focusing in the first dimension: An interlaboratory comparison

1994 • 151 citations

Improved horizontal two‐dimensional electrophoresis with hybrid isoelectric focusing in immobilized pH gradients in the first dimension and laying‐on transfer to the second dimension

1985 • 124 citations

Two‐dimensional electrophoresis of proteins in an immobilized pH 4–12 gradient

1998 • 123 citations

Genotypes and phenotypes

1999 • 102 citations

Strategies for whole microbial genome sequencing and analysis

1997 • 90 citations

Prefractionation of protein samples prior to two‐dimensional electrophoresis

1997 • 82 citations

Temperature‐dependent spot positional variability in two‐dimensional polypeptide patterns

1991 • 81 citations

Identification of Proteins by Mass Spectrometry

2000 • 80 citations

Highly resolving two-dimensional gels for protein sequencing.

1991 • 79 citations

Proteome analysis of Saccharomyces cerevisiae: A methodological outline

1997 • 75 citations

Detection of Proteins on Two-Dimensional Electrophoresis Gels

2000 • 74 citations

pH gradient simulator for electrophoretic techniques in a windows environment

1993 • 73 citations

Two-Dimensional Electrophoresis: The State of the Art and Future Directions

1997 • 72 citations

Amidosulfobetaines, a family of detergents with improved solubilization properties: Application for isoelectric focusing under denaturing conditions

1990 • 67 citations

Two-dimensional electrophoresis with immobilized pH gradients: current state

1993 • 59 citations

Horizontal two‐dimensional electrophoresis with immobilized pH gradients in the first dimension in the presence of nonionic detergent

1987 • 59 citations

IPGMAKER: A program for IBM‐compatible personal computers to create and test recipes for immobilized pH gradients

1990 • 37 citations

Biotechnology International 1990.

1990 • 36 citations

Application of sequential extraction procedures and glycoprotein blotting for the characterization of the 2‐D polypeptide patterns of barley seed proteins

1992 • 36 citations

Two-Dimensional Electrophoresis with Immobilized pH Gradients

2000 • 27 citations

Isoelectric focusing of histones in extremely alkaline immobilized pH gradients: comparison with capillary electrophoresis

1994 • 26 citations

Solubilization of Proteins in Two-Dimensional Electrophoresis

2000 • 24 citations

Suitability of staining techniques for the detection and quantitation of nonhistone high mobility group proteins

1990 • 8 citations