Subproteomics based upon protein cellular location and relative solubilities in conjunction with composite two-dimensional electrophoresis gels

Mass Spectrometric Sequencing of Proteins from Silver-Stained Polyacrylamide Gels

1996 • 8,994 citations

Quantitative analysis of complex protein mixtures using isotope-coded affinity tags

1999 • 4,699 citations

Mass spectrometric identification of proteins from silver-stained polyacrylamide gel: A method for the removal of silver ions to enhance sensitivity

1999 • 896 citations

Extraction of membrane proteins by differential solubilization for separation using two‐dimensional gel electrophoresis

1998 • 499 citations

Protein Identification at the Low Femtomole Level from Silver-Stained Gels Using a New Fritless Electrospray Interface for Liquid Chromatography–Microspray and Nanospray Mass Spectrometry

1998 • 386 citations

Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K‐12

1997 • 341 citations

New zwitterionic detergents improve the analysis of membrane proteins by two‐dimensional electrophoresis

1998 • 334 citations

Two‐dimensional polyacrylamide gel electrophoresis with immobilized pH gradients in the first dimensin (IPG‐Dalt): The state of the art and the controversy of vertical versus horizontal systems

1995 • 266 citations

Recent developments in two-dimensional gel electrophoresis with immobilized pH gradients: Wide pH gradients up to pH 12, longer separation distances and simplified procedures

1999 • 251 citations

Improved protein solubility in two‐dimensional electrophoresis using tributyl phosphine as reducing agent

1998 • 241 citations

Very alkaline immobilized pH gradients for two‐dimensional electrophoresis of ribosomal and nuclear proteins

1997 • 238 citations

Effects of Environmental pH on Membrane Proteins in Borrelia burgdorferi

1999 • 223 citations

Definition of Mycobacterium tuberculosis culture filtrate proteins by two-dimensional polyacrylamide gel electrophoresis, N-terminal amino acid sequencing, and electrospray mass spectrometry

1997 • 222 citations

Proteome research: Complementarity and limitations with respect to the RNA and DNA worlds

1997 • 218 citations

Proteomics and automation

1999 • 209 citations

Two‐dimensional gel electrophoresis for proteome projects: The effects of protein hydrophobicity and copy number

1998 • 199 citations

Diagnosis of cellular states of microbial organisms using proteomics

1999 • 186 citations

Current challenges and future applications for protein maps and post‐translational vector maps in proteome projects

1996 • 183 citations

2D protein electrophoresis: can it be perfected?

1999 • 182 citations

Dual channel imaging of two-dimensional electropherograms inBacillus subtilis

1999 • 158 citations

Positional reproducibility of protein spots in two‐dimensional polyacrylamide gel electrophoresis using immobilised pH gradient isoelectric focusing in the first dimension: An interlaboratory comparison

1994 • 151 citations

Towards the recovery of hydrophobic proteins on two-dimensional electrophoresis gels

1999 • 150 citations

Analysis of membrane proteins by two-dimensional electrophoresis: Comparison of the proteins extracted from normal orPlasmodium falciparum - infected erythrocyte ghosts

1999 • 144 citations

Genomic mapping of Pseudomonas aeruginosa PAO

1994 • 127 citations

Enrichment of low abundance proteins ofEscherichia coli by hydroxyapatite chromatography

1999 • 109 citations

Separation and characterization of rice proteins

1994 • 96 citations

Identification of yeast proteins from two‐dimensional gels: Working out spot cross‐contamination

1998 • 87 citations

Silver Staining of 2-D Electrophoresis Gels

2003 • 86 citations

Enrichment of low-copy-number gene products by hydrophobic interaction chromatography

1999 • 82 citations

Prefractionation of protein samples prior to two‐dimensional electrophoresis

1997 • 82 citations

Low molecular weight proteins: A challenge for post‐genomic research

1998 • 62 citations

Proteomic ‘contigs’ of Ochrobactrum anthropi, application of extensive pH gradients

1997 • 55 citations

Reassembling proteins and chaperones in human nuclear matrix protein fractions

1999 • 48 citations

Isoelectric focusing in immobilized pH gradients: an update

1997 • 43 citations

Comparison of Predicted and Observed Properties of Proteins Encoded in the Genome ofMycobacterium TuberculosisH37Rv

1998 • 43 citations

Small genes/gene-products inEscherichia coliK-12

1998 • 42 citations

Identification and Characterization of the Genes for Mitochondrial Ribosomal Proteins of Saccharomyces Cerevisiae

1997 • 42 citations

Reassembling proteins and chaperones in human nuclear matrix protein fractions

1999 • 42 citations

Characterisation of basic proteins from Spiroplasma melliferum using novel immobilised pH gradients

1997 • 36 citations

The Genome of Staphylococcus aureus: A Review

1998 • 31 citations

Purification and Characterization of the 30S Ribosomal Proteins from the Bacterium Thermus thermophilus

1994 • 24 citations

Preparative isoelectric focusing in multicompartment electrolyzers: Novel, hydrolytically stable and hydrophilic isoelectric membranes

1994 • 24 citations

The microbial proteome database — an automated laboratory catalogue for monitoring protein expression in bacteria

1999 • 16 citations

Differences in the envelope proteins ofChlamydia pneumoniae, Chlamydia trachomatis, andChlamydia psittaci shown by two-dimensional gel electrophoresis

1996 • 15 citations