Identifying the Lipid-Protein Interface of the Torpedo Nicotinic Acetylcholine Receptor: Secondary Structure Implications

PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT

1951 • 319,299 citations

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa

1987 • 11,491 citations

Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.

1977 • 5,640 citations

Elution of proteins from sodium dodecyl sulfate-polyacrylamide gels, removal of sodium dodecyl sulfate, and renaturation of enzymatic activity: Results with sigma subunit of Escherichia coli RNA polymerase, wheat germ DNA topoisomerase, and other enzymes

1980 • 1,256 citations

The distribution of positively charged residues in bacterial inner membrane proteins correlates with the trans-membrane topology

1986 • 861 citations

Nicotinic Acetylcholine Receptor an 9 Å Resolution

1993 • 822 citations

Structural homology of Torpedo californica acetylcholine receptor subunits

1983 • 798 citations

Rings of negatively charged amino acids determine the acetylcholine receptor channel conductance

1988 • 798 citations

Primary structure of α-subunit precursor of Torpedo californica acetylcholine receptor deduced from cDNA sequence

1982 • 730 citations

Acetylcholine Receptor Channel Structure Probed in Cysteine-Substitution Mutants

1992 • 668 citations

Acetylcholine Receptor: Complex of Homologous Subunits

1980 • 594 citations

Primary structures of β- and δ-subunit precursors of Torpedo californica acetylcholine receptor deduced from cDNA sequences

1983 • 527 citations

Nucleotide and deduced amino acid sequences of Torpedo californica acetylcholine receptor gamma subunit.

1983 • 412 citations

Molecular weight in detergent solution of acetylcholine receptor from Torpedo californica

1978 • 384 citations

Evidence That the M2 Membrane-Spanning Region Lines the Ion Channel Pore of the Nicotinic Receptor

1988 • 379 citations

Large‐Scale Purification of the Acetylcholine‐Receptor Protein in Its Membrane‐Bound and Detergent‐Extracted Forms from Torpedo marmorata Electric Organ

1977 • 375 citations

Nicotinic receptor of acetylcholine: structure of an oligomeric integral membrane protein

1984 • 373 citations

The function of tryptophan residues in membrane proteins

1992 • 344 citations

Proline kinks in transmembrane α-helices

1991 • 327 citations

Hydrophobic Organization of Membrane Proteins

1989 • 316 citations

Location of a δ-subunit region determining ion transport through the acetylcholine receptor channel

1986 • 315 citations

Structure of the high-affinity binding site for noncompetitive blockers of the acetylcholine receptor: serine-262 of the delta subunit is labeled by [3H]chlorpromazine.

1986 • 296 citations

An open-channel blocker interacts with adjacent turns of α-helices in the nicotinic acetylcholine receptor

1990 • 275 citations

Nicotinic acetylcholine receptor and superfamily of ligand-gated ion channels

1990 • 260 citations

Structure of nicotinic acetylcholine receptors

1993 • 234 citations

Structure of the reaction center from Rhodobacter sphaeroides R-26: membrane-protein interactions.

1987 • 229 citations

Structure of the high-affinity binding site for noncompetitive blockers of the acetylcholine receptor: [3H]chlorpromazine labels homologous residues in the .beta. and .delta. chains

1987 • 228 citations

Bacteriorhodopsin is an inside-out protein.

1980 • 212 citations

Three-dimensional structure of the acetylcholine receptor by cryoelectron microscopy and helical image reconstruction.

1990 • 197 citations

Mapping of the acetylcholine binding site of the nicotinic acetylcholine receptor: [3H]nicotine as an agonist photoaffinity label

1991 • 184 citations

The functional architecture of the acetylcholine nicotinic receptor explored by affinity labelling and site-directed mutagenesis

1992 • 178 citations

The nicotinic acetylcholine receptor and its ion channel

1986 • 169 citations

Modeling α‐helical transmembrane domains: The calculation and use of substitution tables for lipid‐facing residues

1993 • 151 citations

Location of a threonine residue in the α -subunit M2 transmembrane segment that determines the ion flow through the acetylcholine receptor channel

1991 • 148 citations

Agonist-induced changes in the structure of the acetylcholine receptor M2 regions revealed by photoincorporation of an uncharged nicotinic noncompetitive antagonist.

1992 • 145 citations

Mapping the lipid-exposed regions in the Torpedo californica nicotinic acetylcholine receptor

1992 • 141 citations

Use of o-phthalaldehyde to reduce background during automated Edman degradation

1984 • 138 citations

A ring of uncharged polar amino acids as a component of channel constriction in the nicotinic acetylcholine receptor

1991 • 127 citations

Location of ligand-binding sites on the nicotinic acetylcholine receptor alpha-subunit.

1986 • 119 citations

Explorations of the nicotinic acetylcholine receptor.

1989 • 113 citations

Threonine in the selectivity filter of the acetylcholine receptor channel

1992 • 112 citations

Mapping the alpha-subunit site photolabeled by the noncompetitive inhibitor [3H]quinacrine azide in the active state of the nicotinic acetylcholine receptor.

1990 • 104 citations

Photolabeling of membrane-bound Torpedo nicotinic acetylcholine receptor with the hydrophobic probe 3-trifluoromethyl-3-(m-[125I]iodophenyl)diazirine

1988 • 87 citations

Transmembrane topology of acetylcholine receptor subunits probed with photoreactive phospholipids

1985 • 81 citations

Structure of the noncompetitive antagonist-binding site of the Torpedo nicotinic acetylcholine receptor. [3H]meproadifen mustard reacts selectively with alpha-subunit Glu-262.

1992 • 80 citations

Effects of substitution of putative transmembrane segments on nicotinic acetylcholine receptor function

1987 • 75 citations

The sidedness of the COOH terminus of the acetylcholine receptor δ subunit

1989 • 69 citations

The hydrophobic photoreagent 3-(trifluoromethyl)-3-m-([125I] iodophenyl) diazirine is a novel noncompetitive antagonist of the nicotinic acetylcholine receptor.

1991 • 61 citations

Average membrane penetration depth of tryptophan residues of the nicotinic acetylcholine receptor by the parallax method

1991 • 54 citations

Characterization of the Nicotinic Acetylcholine Receptor β3 Gene

1995 • 50 citations

Site-specific mutations of nicotinic acetylcholine receptor at the lipid-protein interface dramatically alter ion channel gating

1992 • 49 citations

Philicity of amino acid side-chains for photogenerated carbenes

1990 • 35 citations

Reaction of [3H]meproadifen mustard with membrane-bound Torpedo acetylcholine receptor.

1986 • 28 citations

Protein-lipid interactions and Torpedo californica nicotinic acetylcholine receptor function. 1. Spatial disposition of cysteine residues in the .gamma. subunit analyzed by fluorescence-quenching and energy-transfer measurements

1993 • 23 citations

Changes in Conformation upon Agonist Binding, and Nonequivalent Labeling, of the Membrane-spanning Regions of the Nicotinic Acetylcholine Receptor Subunits

1989 • 22 citations

The role of charge in lipid selectivity for the nicotinic acetylcholine receptor

1993 • 22 citations

Covalent modification of a critical sulfhydryl group in the acetylcholine receptor: cysteine-222 of the .alpha.-subunit

1989 • 21 citations

Transverse localization of the quinacrine binding site on the Torpedo acetylcholine receptor.

1993 • 21 citations