FLT3 Ligand Preserves the Ability of Human CD34+ Progenitors to Sustain Long-Term Hematopoiesis in Immune-Deficient Mice After Ex Vivo Retroviral-Mediated Transduction

Improved retroviral vectors for gene transfer and expression.

1989 • 1,997 citations

Gene transfer by retrovirus vectors occurs only in cells that are actively replicating at the time of infection.

1990 • 1,465 citations

Passage through mitosis is required for oncoretroviruses but not for the human immunodeficiency virus

1994 • 798 citations

Introduction of a selectable gene into primitive stem cells capable of long-term reconstitution of the hemopoietic system of W/W mice

1985 • 663 citations

Construction and properties of retrovirus packaging cells based on gibbon ape leukemia virus

1991 • 647 citations

Engraftment of gene–modified umbilical cord blood cells in neonates with adenosine deaminase deficiency

1995 • 622 citations

Expression of a foreign gene in myeloid and lymphoid cells derived from multipotent haematopoietic precursors

1985 • 598 citations

Clonal and systemic analysis of long-term hematopoiesis in the mouse.

1990 • 577 citations

Molecular cloning of a ligand for the flt3flk-2 tyrosine kinase receptor: A proliferative factor for primitive hematopoietic cells

1993 • 546 citations

STK-1, the human homolog of Flk-2/Flt-3, is selectively expressed in CD34+ human bone marrow cells and is involved in the proliferation of early progenitor/stem cells.

1994 • 459 citations

A functional comparison of CD34 + CD38- cells in cord blood and bone marrow

1995 • 456 citations

Ligand for FLT3/FLK2 receptor tyrosine kinase regulates growth of haematopoietic stem cells and is encoded by variant RNAs

1994 • 453 citations

Direct demonstration that autologous bone marrow transplantation for solid tumors can return a multiplicity of tumorigenic cells

1994 • 418 citations

Functional Isolation and Characterization of Human Hematopoietic Stem Cells

1995 • 414 citations

Combination of interleukins 3 and 6 preserves stem cell function in culture and enhances retrovirus-mediated gene transfer into hematopoietic stem cells.

1989 • 316 citations

Mechanisms that regulate the cell cycle status of very primitive hematopoietic cells in long-term human marrow cultures. II. Analysis of positive and negative regulators produced by stromal cells within the adherent layer

1991 • 292 citations

Gene Expression in Mice After High Efficiency Retroviral-Mediated Gene Transfer

1985 • 281 citations

Sustained human hematopoiesis in immunodeficient mice by cotransplantation of marrow stroma expressing human interleukin-3: analysis of gene transduction of long-lived progenitors

1994 • 264 citations

Flt3 ligand induces proliferation of quiescent human bone marrow CD34+CD38- cells and maintains progenitor cells in vitro

1996 • 240 citations

Multi-level effects of flt3 ligand on human hematopoiesis: expansion of putative stem cells and proliferation of granulomonocytic progenitors/monocytic precursors

1995 • 240 citations

Bone marrow extracellular matrix molecules improve gene transfer into human hematopoietic cells via retroviral vectors.

1994 • 228 citations

Multiple modifications in cis elements of the long terminal repeat of retroviral vectors lead to increased expression and decreased DNA methylation in embryonic carcinoma cells

1995 • 211 citations

Transduction of pluripotent human hematopoietic stem cells demonstrated by clonal analysis after engraftment in immune-deficient mice.

1996 • 186 citations

Novel use of polymerase chain reaction to amplify cellular DNA adjacent to an integrated provirus

1989 • 186 citations

Analysis of optimal conditions for retroviral-mediated transduction of primitive human hematopoietic cells

1995 • 182 citations

The FLT3 ligand potently and directly stimulates the growth and expansion of primitive murine bone marrow progenitor cells in vitro: synergistic interactions with interleukin (IL) 11, IL-12, and other hematopoietic growth factors.

1995 • 175 citations

Flt3 ligand stimulates/costimulates the growth of myeloid stem/progenitor cells.

1995 • 168 citations

Effect of flt3 ligand on the ex vivo expansion of human CD34+ hematopoietic progenitor cells

1995 • 159 citations

Retroviral-mediated gene transfer into CD34-enriched human peripheral blood stem cells.

1993 • 154 citations

Stromal support enhances cell-free retroviral vector transduction of human bone marrow long-term culture-initiating cells

1992 • 141 citations

Comparison of the Effects of Growth Factors on Retroviral Vector-Mediated Gene Transfer and the Proliferative Status of Human Hematopoietic Progenitor Cells

1990 • 133 citations

FLT3/FLK2 ligand promotes the growth of murine stem cells and the expansion of colony-forming cells and spleen colony-forming units

1995 • 129 citations

Human cord blood cells as targets for gene transfer: potential use in genetic therapies of severe combined immunodeficiency disease.

1993 • 122 citations

Growth factors increase amphotropic retrovirus binding to human CD34+ bone marrow progenitor cells

1993 • 116 citations

High efficiency retroviral mediated gene transduction into single isolated immature and replatable CD34(3+) hematopoietic stem/progenitor cells from human umbilical cord blood.

1993 • 106 citations

Stromal support enhances cell-free retroviral vector transduction of human bone marrow long-term culture-initiating cells

1992 • 93 citations

Growth factors and stromal support generate very efficient retroviral transduction of peripheral blood CD34+ cells from Gaucher patients

1995 • 78 citations

Retroviral-mediated transfer of the human glucocerebrosidase gene into cultured Gaucher bone marrow.

1992 • 76 citations

Leukemia inhibitory factor improves survival of retroviral vector-infected hematopoietic stem cells in vitro, allowing efficient long-term expression of vector-encoded human adenosine deaminase in vivo.

1991 • 50 citations

Normal cycling patterns of hematopoietic stem cell subpopulations: an assay using long-term in vivo BrdU infusion

1985 • 44 citations

The presence of an autologous marrow stromal cell layer increases glucocerebrosidase gene transduction of long-term culture initiating cells (LTCICs) from the bone marrow of a patient with Gaucher disease.

1995 • 34 citations