A Large PEST-like Sequence Directs the Ubiquitination, Endocytosis, and Vacuolar Degradation of the Yeast a-Factor Receptor

A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.

1989 • 8,705 citations

Rapid and efficient site-specific mutagenesis without phenotypic selection.

1985 • 7,573 citations

[19] Rapid and efficient site-specific mutagenesis without phenotypic selection

1987 • 5,413 citations

Amino Acid Sequences Common to Rapidly Degraded Proteins: The PEST Hypothesis

1986 • 2,566 citations

The ubiquitin-proteasome proteolytic pathway

1994 • 1,778 citations

UBIQUITIN-DEPENDENT PROTEIN DEGRADATION

1996 • 1,680 citations

PEST sequences and regulation by proteolysis

1996 • 1,606 citations

ENDOCYTOSIS AND MOLECULAR SORTING

1996 • 1,537 citations

[10] 5-Fluoroorotic acid as a selective agent in yeast molecular genetics

1987 • 1,375 citations

A Saccharomyces cerevisiae genomic plasmid bank based on a centromere-containing shuttle vector

1987 • 1,181 citations

CLATHRIN-COATED VESICLE FORMATION AND PROTEIN SORTING: An Integrated Process

1997 • 793 citations

Ubiquitination of a Yeast Plasma Membrane Receptor Signals Its Ligand-Stimulated Endocytosis

1996 • 789 citations

Ubiquitin Lys63 is involved in ubiquitination of a yeast plasma membrane protein

1997 • 398 citations

end3 and end4: two mutants defective in receptor-mediated and fluid-phase endocytosis in Saccharomyces cerevisiae.

1993 • 380 citations

A Function for Monoubiquitination in the Internalization of a G Protein–Coupled Receptor

1998 • 364 citations

NPI1, an essential yeast gene involved in induced degradation of Gap1 and Fur4 permeases, encodes the Rsp5 ubiquitin—protein ligase

1995 • 331 citations

The ABC-transporter Ste6 accumulates in the plasma membrane in a ubiquitinated form in endocytosis mutants.

1994 • 319 citations

Ubiquitination Mediated by the Npi1p/Rsp5p Ubiquitin-protein Ligase Is Required for Endocytosis of the Yeast Uracil Permease

1996 • 316 citations

The ubiquitin conjugation system is required for ligand-induced endocytosis and degradation of the growth hormone receptor.

1996 • 307 citations

Evidence the yeast STE3 gene encodes a receptor for the peptide pheromone a factor: gene sequence and implications for the structure of the presumed receptor.

1986 • 305 citations

Mannose 6–Phosphate Receptors Are Sorted from Immature Secretory Granules via Adaptor Protein AP-1, Clathrin, and Syntaxin 6–positive Vesicles

1998 • 290 citations

Cytoplasmic Tail Phosphorylation of the α-Factor Receptor Is Required for Its Ubiquitination and Internalization

1998 • 276 citations

Cis- and trans-acting functions required for endocytosis of the yeast pheromone receptors

1993 • 239 citations

Epitope-tagged ubiquitin. A new probe for analyzing ubiquitin function.

1991 • 212 citations

Immunocytochemical visualization of coated pits and vesicles in human fibroblasts: Relation to low density lipoprotein receptor distribution

1978 • 181 citations

Ubiquitination of the yeast a-factor receptor.

1996 • 172 citations

Protein transport to the vacuole and receptor-mediated endocytosis by clathrin heavy chain-deficient yeast.

1988 • 153 citations

Clathrin facilitates the internalization of seven transmembrane segment receptors for mating pheromones in yeast.

1993 • 151 citations

Yeast peptide pheromones, a-factor and α-factor, activate a common response mechanism in their target cells

1986 • 137 citations

A PEST-Like Sequence Mediates Phosphorylation and Efficient Ubiquitination of Yeast Uracil Permease

1998 • 127 citations

Identification of a novel sequence mediating regulated endocytosis of the G protein-coupled alpha-pheromone receptor in yeast.

1993 • 126 citations

The yeast multidrug transporter Pdr5 of the plasma membrane is ubiquitinated prior to endocytosis and degradation in the vacuole

1996 • 124 citations

The sequence NPFXD defines a new class of endocytosis signal in Saccharomyces cerevisiae.

1996 • 109 citations

Linkage of the ubiquitin-conjugating system and the endocytic pathway in ligand-induced internalization of the growth hormone receptor

1997 • 100 citations

Actin-, myosin- and ubiquitin-dependent endocytosis

1996 • 94 citations

The linker region of the ABC-transporter Ste6 mediates ubiquitination and fast turnover of the protein

1997 • 79 citations

Half-life of the plasma membrane ATPase and its activating system in resting yeast cells

1991 • 74 citations

MDP1, a Saccharomyces cerevisiae Gene Involved in Mitochondrial/Cytoplasmic Protein Distribution, Is Identical to the Ubiquitin-Protein Ligase Gene RSP5

1997 • 70 citations

Dominant lethal mutations in the plasma membrane H(+)-ATPase gene of Saccharomyces cerevisiae.

1994 • 69 citations

Role for the Ubiquitin-Proteasome System in the Vacuolar Degradation of Ste6p, the a-Factor Transporter in Saccharomyces cerevisiae

1998 • 64 citations

Catabolite inactivation of the yeast maltose transporter requires ubiquitin-ligase npi1/rsp5 and ubiquitin-hydrolase npi2/doa4

2006 • 64 citations

Mutations that alter the third cytoplasmic loop of the a-factor receptor lead to a constitutive and hypersensitive phenotype.

1993 • 63 citations

The ankyrin repeat-containing protein Akr1p is required for the endocytosis of yeast pheromone receptors.

1997 • 49 citations

Response of Yeast Cells to a-Factor Pheromone: Topology of the Receptor and Identification of a Component of the Response Pathway

1988 • 25 citations