The G-Protein Family and Their Interaction with Receptors*

G PROTEINS: TRANSDUCERS OF RECEPTOR-GENERATED SIGNALS

1987 • 6,374 citations

Isolation of two proteins with high affinity for guanine nucleotides from membranes of bovine brain.

1984 • 1,300 citations

Cloning of the gene and cDNA for mammalian β-adrenergic receptor and homology with rhodopsin

1986 • 1,264 citations

Role of guanine nucleotide binding protein in the activation of polyphosphoinositide phosphodiesterase

1985 • 960 citations

Cloning, sequencing and expression of complementary DNA encoding the muscarinic acetylcholine receptor

1986 • 917 citations

Cloning, Sequencing, and Expression of the Gene Coding for the Human Platelet α ₂ -Adrenergic Receptor

1987 • 792 citations

Cloning and expression of the human and rat m5 muscarinic acetylcholine receptor genes

1988 • 792 citations

Phosphodiesterase activation by photoexcited rhodopsin is quenched when rhodopsin is phosphorylated and binds the intrinsic 48-kDa protein of rod outer segments.

1986 • 781 citations

Chimeric α ₂ -,β ₂ -Adrenergic Receptors: Delineation of Domains Involved in Effector Coupling and Ligand Binding Specificity

1988 • 766 citations

Distinct primary structures, ligand-binding properties and tissue-specific expression of four human muscarinic acetylcholine receptors.

1987 • 766 citations

Molecular cloning of five GTP-binding protein cDNA species from rat olfactory neuroepithelium.

1987 • 751 citations

Flow of information in the light-triggered cyclic nucleotide cascade of vision.

1981 • 740 citations

Protein kinase C phosphorylates the inhibitory guanine‐nucleotide‐binding regulatory component and apparently suppresses its function in hormonal inhibition of adenylate cyclase

1985 • 736 citations

Isolation, sequence analysis, and intron-exon arrangement of the gene encoding bovine rhodopsin

1983 • 689 citations

cDNA for the human beta 2-adrenergic receptor: a protein with multiple membrane-spanning domains and encoded by a gene whose chromosomal location is shared with that of the receptor for platelet-derived growth factor.

1987 • 687 citations

Regulation of transmembrane signaling by receptor phosphorylation

1987 • 678 citations

Beta-adrenergic receptor kinase: identification of a novel protein kinase that phosphorylates the agonist-occupied form of the receptor.

1986 • 650 citations

Lipoxygenase metabolites of arachidonic acid as second messengers for presynaptic inhibition of Aplysia sensory cells

1987 • 640 citations

A family of receptors coupled to guanine nucleotide regulatory proteins

1987 • 636 citations

Structure of the GDP Domain of EF-Tu and Location of the Amino Acids Homologous to ras Oncogene Proteins

1985 • 626 citations

G protein involvement in receptor-effector coupling.

1988 • 618 citations

Intrinsic GTPase activity distinguishes normal and oncogenic ras p21 molecules.

1984 • 607 citations

Cloning of the cDNA for the human beta 1-adrenergic receptor.

1987 • 577 citations

A G Protein Directly Regulates Mammalian Cardiac Calcium Channels

1987 • 551 citations

The structure of bovine rhodopsin

1983 • 513 citations

Primary Structure and Biochemical Properties of an M ₂ Muscarinic Receptor

1987 • 510 citations

The regulatory component of adenylate cyclase. Purification and properties.

1981 • 505 citations

Homologies Between Signal Transducing G Proteins and ras Gene Products

1984 • 500 citations

Characterization of transducin from bovine retinal rod outer segments. I. Separation and reconstitution of the subunits.

1983 • 492 citations

The STE4 and STE18 genes of yeast encode potential β and γ subunits of the mating factor receptor-coupled G protein

1989 • 491 citations

Functional desensitization of the isolated beta-adrenergic receptor by the beta-adrenergic receptor kinase: potential role of an analog of the retinal protein arrestin (48-kDa protein).

1987 • 478 citations

Pertussis toxin-catalyzed ADP-ribosylation of transducin. Cysteine 347 is the ADP-ribose acceptor site.

1985 • 457 citations

G-protein βγ-subunits activate the cardiac muscarinic K+-channel via phospholipase A2

1989 • 438 citations

The yeast SCG1 gene: A Gα-like protein implicated in the a- and α-factor response pathway

1987 • 431 citations

5-Hydroxytryptamine stimulates inositol phosphate production in a cell-free system from blowfly salivary glands. Evidence for a role of GTP in coupling receptor activation to phosphoinositide breakdown.

1985 • 429 citations

Removal of phosphorylation sites from the β2-adrenergic receptor delays onset of agonist-promoted desensitization

1988 • 429 citations

Repetitive segmental structure of the transducin beta subunit: homology with the CDC4 gene and identification of related mRNAs.

1986 • 411 citations

Purification of a protein cofactor required for ADP-ribosylation of the stimulatory regulatory component of adenylate cyclase by cholera toxin.

1984 • 410 citations

Site-directed mutagenesis of the cytoplasmic domains of the human beta 2-adrenergic receptor. Localization of regions involved in G protein-receptor coupling.

1988 • 400 citations

ADP-ribosylation of the specific membrane protein by islet-activating protein, pertussis toxin, associated with inhibition of a chemotactic peptide-induced arachidonate release in neutrophils. A possible role of the toxin substrate in Ca2+-mobilizing biosignaling.

1984 • 389 citations

Hormone-stimulated polyphosphoinositide breakdown in rat liver plasma membranes. Roles of guanine nucleotides and calcium.

1986 • 378 citations

Human cDNA clones for four species of G alpha s signal transduction protein.

1986 • 370 citations

GPA1, a haploid-specific essential gene, encodes a yeast homolog of mammalian G protein which may be involved in mating factor signal transduction

1987 • 365 citations

Stimulation of phospholipase A2 activity in bovine rod outer segments by the beta gamma subunits of transducin and its inhibition by the alpha subunit.

1987 • 344 citations

ADP-ribosylation of transducin by pertussis toxin blocks the light-stimulated hydrolysis of GTP and cGMP in retinal photoreceptors.

1984 • 327 citations

Ligand binding to the β-adrenergic receptor involves its rhodopsin-like core

1987 • 318 citations

Phosphorylation of the mammalian beta-adrenergic receptor by cyclic AMP-dependent protein kinase. Regulation of the rate of receptor phosphorylation and dephosphorylation by agonist occupancy and effects on coupling of the receptor to the stimulatory guanine nucleotide regulatory protein.

1985 • 312 citations

The G protein-gated atrial K+ channel is stimulated by three distinct GIα-subunits

1988 • 306 citations

Arachidonic acid metabolites as intracellular modulators of the G protein-gated cardiac K+ channel

1989 • 299 citations

Mutant ras-encoded proteins with altered nucleotide binding exert dominant biological effects.

1986 • 285 citations

Sequence of the Alpha Subunit of Photoreceptor G Protein: Homologies Between Transducin, ras , and Elongation Factors

1985 • 276 citations

Myristoylated alpha subunits of guanine nucleotide-binding regulatory proteins.

1987 • 276 citations

Presence of three distinct molecular species of Gi protein alpha subunit. Structure of rat cDNAs and human genomic DNAs.

1988 • 275 citations

A single amino acid substitution in rhodopsin (lysine 248—-leucine) prevents activation of transducin.

1988 • 260 citations

Mutations that uncouple the beta-adrenergic receptor from Gs and increase agonist affinity.

1987 • 259 citations

The avian beta-adrenergic receptor: primary structure and membrane topology.

1986 • 252 citations

Identification of a GTP-binding protein alpha subunit that lacks an apparent ADP-ribosylation site for pertussis toxin.

1988 • 251 citations

Identification of receptor contact site involved in receptor–G protein coupling

1987 • 249 citations

Receptor-mediated inhibition of adenylate cyclase and stimulation of arachidonic acid release in 3T3 fibroblasts. Selective susceptibility to islet-activating protein, pertussis toxin.

1985 • 245 citations

The opsin family of proteins

1986 • 245 citations

Purification and characterization of the beta-adrenergic receptor kinase.

1987 • 241 citations

Enhancement of adenylate cyclase activity in S49 lymphoma cells by phorbol esters. Putative effect of C kinase on alpha s-GTP-catalytic subunit interaction.

1985 • 239 citations

Phorbol diesters promote β-adrenergic receptor phosphorylation and adenylate cyclase desensitization in duck erythrocytes

1984 • 238 citations

Newly Identified Brain Potassium Channels Gated by the Guanine Nucleotide Binding Protein G _o

1988 • 236 citations

Amino acid sequence of retinal transducin at the site ADP-ribosylated by cholera toxin.

1984 • 235 citations

Phorbol ester induces desensitization of adenylate cyclase and phosphorylation of the beta-adrenergic receptor in turkey erythrocytes.

1984 • 233 citations

Kinetics of Activation of Phospholipase C by P2Y Purinergic Receptor Agonists and Guanine Nucleotides

1989 • 206 citations

Family of G protein α chains: amphipathic analysis and predicted structure of functional domains

1986 • 197 citations

Inactivation of photoexcited rhodopsin in retinal rods: the roles of rhodopsin kinase and 48-kDa protein (arrestin)

1988 • 185 citations

Differential G protein—mediated coupling of neurotransmitter receptors to Ca2+ channels in rat dorsal root ganglion neurons in vitro

1989 • 180 citations

A mutation that prevents GTP-dependent activation of the α chain of Gs

1988 • 176 citations

Carboxyl Rerminal Domain of G _sα Specifies Coupling of Receptors to Stimulation of Adenylyl Cyclase

1988 • 174 citations

Chapter 5 Interactions between photoexcited rhodopsin and light-activated enzymes in rods

1984 • 174 citations

Adenylate cyclase permanently uncoupled from hormone receptors in a novel variant of S49 mouse lymphoma cells.

1977 • 173 citations

Location of a region of the muscarinic acetylcholine receptor involved in selective effector coupling

1988 • 167 citations

Phosphorylation of rhodopsin and quenching of cyclic GMP phosphodiesterase activation by ATP at weak bleaches.

1983 • 165 citations

Structural basis of beta-adrenergic receptor subtype specificity studied with chimeric beta 1/beta 2-adrenergic receptors.

1988 • 160 citations

Activation of a G protein promotes agonist responses to calcium channel ligands

1987 • 160 citations

Antisera against a guanine nucleotide binding protein from retina cross-react with the beta subunit of the adenylyl cyclase-associated guanine nucleotide binding proteins, Ns and Ni.

1985 • 160 citations

Signal transduction by G proteins

1990 • 159 citations

Site-directed mutagenesis and continuous expression of human beta-adrenergic receptors. Identification of a conserved aspartate residue involved in agonist binding and receptor activation.

1988 • 158 citations

Activation of the Cellular Proto-Oncogene Product p21Ras by Addition of a Myristylation Signal

1989 • 158 citations

Agonist-dependent phosphorylation of the alpha 2-adrenergic receptor by the beta-adrenergic receptor kinase.

1987 • 156 citations

Primary structure of the β‐subunit of bovine transducin deduced from the cDNA sequence

1985 • 155 citations

Angiotensin II Receptors in Bovine Adrenal Cortex

1974 • 147 citations

The carboxyl terminus of the hamster β-adrenergic receptor expressed in mouse L cells is not required for receptor sequestration

1987 • 142 citations

Light-induced binding of guanosinetriphosphatase to bovine photoreceptor membranes: effect of limited proteolysis of the membranes

1981 • 136 citations

Angiotensin II-induced stimulation of voltage-dependent Ca2+ currents in an adrenal cortical cell line.

1988 • 134 citations

Characterization of transducin from bovine retinal rod outer segments. Participation of the amino-terminal region of T alpha in subunit interaction.

1987 • 131 citations

Site-directed mutagenesis of human beta-adrenergic receptors: substitution of aspartic acid-130 by asparagine produces a receptor with high-affinity agonist binding that is uncoupled from adenylate cyclase.

1988 • 131 citations

Light-dependent phosphorylation of rhodopsin in living frogs

1974 • 128 citations

Regional homology in GTP-binding proto-oncogene products and elongation factors.

1983 • 123 citations

β‐Subunits of the human liver G_s/G_i signal‐transducing proteins and those of bovine retinal rod cell transducin are identical

1986 • 119 citations

Inhibition of beta-adrenergic receptor kinase prevents rapid homologous desensitization of beta 2-adrenergic receptors.

1989 • 117 citations

Characterization by two-dimensional peptide mapping of the gamma subunits of Ns and Ni, the regulatory proteins of adenylyl cyclase, and of transducin, the guanine nucleotide-binding protein of rod outer segments of the eye.

1985 • 114 citations

Phosphorylation of the cardiac muscarinic receptor in intact chick heart and its regulation by a muscarinic agonist.

1986 • 110 citations

Allosteric behavior in transducin activation mediated by rhodopsin. Initial rate analysis of guanine nucleotide exchange.

1987 • 105 citations

Correlation of agonist-induced phosphorylation of chick heart muscarinic receptors with receptor desensitization.

1987 • 98 citations

Guanine nucleotide regulation of agonist binding to muscarinic cholinergic receptors. Relation to efficacy of agonists for stimulation of phosphoinositide breakdown and Ca2+ mobilization

1985 • 96 citations

The regulatory component of adenylate cyclase from uncoupled S49 lymphoma cells differs in charge from the wild type protein.

1980 • 92 citations

Guanine nucleotide-dependent pertussis-toxin-insensitive stimulation of inositol phosphate formation by carbachol in a membrane preparation from human astrocytoma cells

1986 • 88 citations

The hydrophobic tryptic core of the beta-adrenergic receptor retains Gs regulatory activity in response to agonists and thiols.

1987 • 87 citations

Receptor activation of G proteins

1988 • 86 citations

Molecular model of the G protein alpha subunit based on the crystal structure of the HRAS protein.

1989 • 82 citations

Differential effect of pertussis toxin on the affinity state for agonists of renal alpha 1- and alpha 2- adrenoceptors.

1984 • 79 citations

Guanine nucleotide-sensitive, high affinity binding of carbachol to muscarinic cholinergic receptors of 1321N1 astrocytoma cells is insensitive to pertussis toxin.

1985 • 78 citations

Epidermal growth factor (EGF) and hormones stimulate phosphoinositide hydrolysis and increase EGF receptor protein synthesis and mRNA levels in rat liver epithelial cells. Evidence for protein kinase C-dependent and -independent pathways.

1988 • 76 citations

Beta gamma subunits of GTP-binding proteins inhibit muscarinic receptor stimulation of phospholipase C.

1988 • 74 citations

Platelet-derived growth factor receptors expressed by cDNA transfection couple to a diverse group of cellular responses associated with cell proliferation.

1988 • 73 citations

Topographic analysis of antigenic determinants recognized by monoclonal antibodies to the photoreceptor guanyl nucleotide-binding protein, transducin.

1987 • 68 citations

Transducin inhibition of light-dependent rhodopsin phosphorylation: evidence for beta gamma subunit interaction with rhodopsin.

1988 • 61 citations

Phosphorylation at sites near rhodopsin'scarboxyl-terminus regulates light initiated CGMP hydrolysis

1984 • 60 citations

Who carries what message?

1989 • 59 citations

Role of two different guanine nucleotide-binding proteins in the antagonistic modulation of the S-type K+ channel by cAMP and arachidonic acid metabolites in Aplysia sensory neurons.

1988 • 58 citations

Transducin interactions with rhodopsin. Evidence for positive cooperative behavior.

1987 • 48 citations

GTP binding protein: Properties and lack of activation by phosphorylated rhodopsin

1984 • 48 citations

The G protein beta 2 complementary DNA encodes the beta 35 subunit.

1987 • 47 citations

Expression of a Gαs/Gαi Chimera That Constitutively Activates Cyclic AMP Synthesis

1989 • 45 citations

A structural model for the α‐subunit of transducin Implications of its role as a molecular switch in the visual signal transduction mechanism

1987 • 36 citations

Kinetic and hydrodynamic properties of transducin: comparison of physical and structural parameters for GTP-binding regulatory proteins

1987 • 34 citations

Immunochemical detection of the alpha-subunit of the G-protein, GZ, in membranes and cytosols of mammalian cells

1988 • 28 citations

Mutation of glycine 49 to valine in the .alpha. subunit of Gs results in the constitutive elevation of cyclic AMP synthesis

1989 • 24 citations

Enzymatic model for receptor activation of GTP-binding regulatory proteins

1987 • 23 citations

Exploring visual transduction with recombinant DNA techniques

1986 • 23 citations

Production of antibodies against rhodopsin after immunization with .beta..gamma.-subunits of transducin: evidence for interaction of .beta..gamma.-subunits of guanosine 5'-triphosphate binding proteins with receptor

1987 • 16 citations

Evidence for oligomeric forms of transducins alpha subunit: Formation of intermolecular alpha-alpha disulfide linkages

1989 • 16 citations