Synchronisation of cancer cell lines of human origin using methotrexate

Establishment and characterization of a cloned line of C3H mouse embryo cells sensitive to postconfluence inhibition of division.

1973 • 1,126 citations

Aphidicolin prevents mitotic cell division by interfering with the activity of DNA polymerase-α

1978 • 904 citations

High Resolution of Human Chromosomes

1976 • 786 citations

The Pharmacology and Clinical Use of Methotrexate

1983 • 769 citations

A method to measure the duration of DNA syntheses and the potential doubling time from a single sample

1985 • 599 citations

The incorporation of 3H-cytosine arabinoside and its effect on murine leukemic cells (L5178Y)

1968 • 296 citations

Synchronization of HeLa cell cultures by inhibition of DNA polymerase a with aphidicolin

1980 • 291 citations

The cellular pharmacology of methotrexate

1985 • 254 citations

Correlation between cell cycle duration and RNA content

1979 • 157 citations

THE BIOCHEMICAL, CELLULAR, AND PHARMACOLOGICAL ACTION AND EFFECTS OF THE FOLIC ACID ANTAGONISTS.

1963 • 156 citations

Correlated Measurements of DNA, RNA, and Protein in Individual Cells by Flow Cytometry

1985 • 153 citations

Cytochemistry for bromodeoxyuridine/DNA analysis: Stoichiometry and sensitivity

1985 • 150 citations

Biochemical effects of a quinazoline inhibitor of thymidylate synthetase, N-(4-(N-((2-amino-4-hydroxy-6-quinazolinyl)methyl)prop-2-ynylamino)benzoyl)-l-glutamic acid (CB3717), on human lymphoblastoid cells

1983 • 113 citations

Uptake as a Determinant of Methotrexate Response in Mouse Leukemias

1965 • 107 citations

Effect of adriamycin on the cell cycle traverse and kinetics of cultured human lymphoblasts.

1976 • 102 citations

[20] Cell synchronization

1979 • 86 citations

Accumulation of DNA strand breaks in cells exposed to methotrexate or N10-propargyl-5,8-dideazafolic acid.

1988 • 81 citations

Role of thymidylate synthetase activity in development of methotrexate cytotoxicity.

1979 • 81 citations

Biological effects of folic acid antagonists with antineoplastic activity

1984 • 77 citations

Synchronization of human diploid fibroblasts at multiple stages of the cell cycle*1

1988 • 76 citations

Comparison of synchronized Chinese hamster ovary cells obtained by mitotic shake‐off, hydroxyurea, aphidicolin, or methotrexate

1987 • 72 citations

The ratio of RNA to total nucleic acid content as a quantitative measure of unbalanced cell growth

1982 • 61 citations

Role of methylenetetrahydrofolate depletion in methotrexate-mediated intracellular thymidylate synthesis inhibition in cultured L1210 cells.

1988 • 57 citations

Normal and perturbed Chinese hamster ovary cells: correlation of DNA, RNA, and protein content by flow cytometry.

1985 • 55 citations

Simplified method for DNA and protein staining of human hematopoietic cell samples

1981 • 49 citations

Kinetics of DNA replication in C3H 10T1/2 cells synchronized by aphidicolin

1985 • 38 citations

Flow cytometric analysis by bromodeoxyuridine/DNA assay of cell cycle perturbation of methotrexate-treated mouse L1210 leukemia cells.

1988 • 34 citations

Time-dependent changes in H1 content, H1 turnover, DNA elongation, and the survival of cells blocked in early S phase by hydroxyurea, aphidicolin, or 5-fluorodeoxyuridine

1985 • 28 citations

Cell Synchrony Techniques

1987 • 27 citations

Dose-related effects of methotrexate on purine and pyrimidine nucleotides and on cell-kinetic parameters in molt-4 malignant human T-lymphoblasts

1986 • 24 citations

Effects of cholera toxin and isobutylmethylxanthine on growth of human fibroblasts

1986 • 21 citations

Flow-cytometric analysis of DNA distribution after VP16-213 treatment of Lewis lung carcinoma

1983 • 17 citations

Enhanced cytotoxicity with methotrexate in conjunction with hypoxanthine in L1210 cells in culture

1988 • 17 citations

Increase in etoposide-induced topoisomerase II-mediated DNA breaks after cell synchronization induced by low doses of methotrexate

1988 • 9 citations