Natural protein proteinase inhibitors and their interaction with proteinases

On the size of the active site in proteases. I. Papain

1967 • 5,150 citations

Matrix metalloproteinases and their inhibitors in connective tissue remodeling

1991 • 3,309 citations

Protein Inhibitors of Proteinases

1980 • 2,220 citations

HUMAN PLASMA PROTEINASE INHIBITORS

1983 • 2,129 citations

Implications of the three-dimensional structure of .alpha.1-antitrypsin for structure and function of serpins

1989 • 898 citations

The refined 1.9 A crystal structure of human alpha-thrombin: interaction with D-Phe-Pro-Arg chloromethylketone and significance of the Tyr-Pro-Pro-Trp insertion segment.

1989 • 889 citations

The structure of protein-protein recognition sites.

1990 • 825 citations

The cystatins: Protein inhibitors of cysteine proteinases

1991 • 813 citations

Human α1-proteinase inhibitor

1984 • 773 citations

The Structure of a Complex of Recombinant Hirudin and Human α-Thrombin

1990 • 718 citations

α-Macroglobulins: structure, shape, and mechanism of proteinase complex formation

1989 • 673 citations

Structural basis of the activation and action of trypsin

1978 • 669 citations

Solution conformation of proteinase inhibitor IIA from bull seminal plasma by 1H nuclear magnetic resonance and distance geometry

1985 • 610 citations

The 2.0 A X-ray crystal structure of chicken egg white cystatin and its possible mode of interaction with cysteine proteinases.

1988 • 584 citations

Kinetics of the inhibition of thrombin by hirudin

1986 • 581 citations

The geometry of the reactive site and of the peptide groups in trypsin, trypsinogen and its complexes with inhibitors

1983 • 580 citations

Protein structures in solution by nuclear magnetic resonance and distance geometry

1987 • 569 citations

Structure of the complex formed by bovine trypsin and bovine pancreatic trypsin inhibitor

1974 • 548 citations

Crystallographic refinement of the structure of bovine pancreatic trypsin inhibitor at l.5 Å resolution

1975 • 544 citations

The refined 2.4 A X-ray crystal structure of recombinant human stefin B in complex with the cysteine proteinase papain: a novel type of proteinase inhibitor interaction.

1990 • 492 citations

Crystal structure of the complex of porcine trypsin with soybean trypsin inhibitor (Kunitz) at 2.6 Å resolution

1974 • 431 citations

Tick Anticoagulant Peptide (TAP) Is a Novel Inhibitor of Blood Coagulation Factor Xa

1990 • 414 citations

Crystal structure of ovalbumin as a model for the reactive centre of serpins

1990 • 394 citations

α1-Antitrypsin and the serpins: variation and countervariation

1985 • 384 citations

Refined structure of the Hirudin-thrombin complex

1991 • 370 citations

Crystal structure of the thrombin-hirudin complex: a novel mode of serine protease inhibition.

1990 • 362 citations

Binding of chloromethyl ketone substrate analogs to crystalline papain

1976 • 352 citations

Determination of Three-Dimensional Structures of Proteins and Nucleic Acids in Solution by Nuclear Magnetic Resonance Spectroscop

1989 • 342 citations

Kinetic role of a meta-stable native-like two-disulphide species in the folding transition of bovine pancreatic trypsin inhibitor

1984 • 308 citations

Structural comparison of two serine proteinase-protein inhibitor complexes: Eglin-C-subtilisin Carlsberg and CI-2-subtilisin Novo

1988 • 307 citations

The transition of bovine trypsinogen to a trypsin-like state upon strong ligand binding

1978 • 301 citations

The high‐resolution X‐ray crystal structure of the complex formed between subtilisin Carlsberg and eglin c, an elastase inhibitor from the leech Hirudo medicinalis

1987 • 289 citations

Molecular and cellular basis of digestion

1986 • 288 citations

X-ray crystal structure of the complex of human leukocyte elastase (PMN elastase) and the third domain of the turkey ovomucoid inhibitor.

1986 • 272 citations

Crystal and molecular structure of the serine proteinase inhibitor CI-2 from barley seeds

1987 • 260 citations

Refined crystal structure of the potato inhibitor complex of carboxypeptidase A at 2.5 Å resolution

1982 • 260 citations

Plakalbumin, α1-antitrypsin, antithrombin and the mechanism of inflammatory thrombosis

1985 • 255 citations

Crystal structure of bovine trypsinogen at 1·8 Å resolution

1977 • 242 citations

Three-dimensional structure of potato carboxypeptidase inhibitor in solution. A study using nuclear magnetic resonance, distance geometry, and restrained molecular dynamics

1987 • 235 citations

Analysis of the plasma elimination kinetics and conformational stabilities of native, proteinase-complexed and reactive site cleaved serpins: comparison of .alpha.1-proteinase inhibitor, .alpha.1-antichymotrypsin, antithrombin III, .alpha.2-antiplasmin, angiotensinogen, and ovalbumin

1991 • 217 citations

The 2.5 A X-ray crystal structure of the acid-stable proteinase inhibitor from human mucous secretions analysed in its complex with bovine alpha-chymotrypsin.

1988 • 216 citations

Crystal and molecular structures of the complex of α-chymotrypsin with its inhibitor Turkey ovomucoid third domain at 1.8 Å resolution

1987 • 215 citations

Structures of product and inhibitor complexes of Streptomyces griseus protease A at 1.8 Å resolution

1980 • 214 citations

Identification of the probable inhibitory reactive sites of the cysteine proteinase inhibitors human cystatin C and chicken cystatin.

1987 • 212 citations

Comparison of two highly refined structures of bovine pancreatic trypsin inhibitor

1987 • 210 citations

Plasma serine proteinase inhibitors (serpins) exhibit major conformational changes and a large increase in conformational stability upon cleavage at their reactive sites.

1988 • 205 citations

Ovomucoid third domains from 100 avian species: isolation, sequences, and hypervariability of enzyme-inhibitor contact residues

1987 • 202 citations

Structure of form III crystals of bovine pancreatic trypsin inhibitor

1987 • 200 citations

Refined 2.5 Å X-ray crystal structure of the complex formed by porcine kallikrein A and the bovine pancreatic trypsin inhibitor

1983 • 199 citations

Structural transition of α₁‐antitrypsin by a peptide sequentially similar to β‐strand s4A

1990 • 197 citations

Structure of the complex of Streptomyces griseus protease B and the third domain of the turkey ovomucoid inhibitor at 1.8-.ANG. resolution

1983 • 193 citations

RIBBON: a stereo cartoon drawing program for proteins

1988 • 190 citations

Three-dimensional structure of the complex between pancreatic secretory trypsin inhibitor (Kazal type) and trypsinogen at 1.8 Å resolution

1982 • 186 citations

The transition of bovine trypsinogen to a trypsin-like state upon strong ligand binding

1979 • 184 citations

The refined 2.0 Å X‐ray crystal structure of the complex formed between bovine β‐trypsin and CMTI‐I, a trypsin inhibitor from squash seeds (Cucurbita maxima) Topological similarity of the squash seed inhibitors with the carboxypeptidase A inhibitor from potatoes

1989 • 181 citations

Mechanism of inhibition of papain by chicken egg white cystatin

1989 • 173 citations

X-ray crystallographic study of the binding of peptide chloromethyl ketone inhibitors to subtilisin BPN

1972 • 168 citations

Cloning, expression, purification, and biological activity of recombinant native and variant human alpha 1-antichymotrypsins.

1990 • 168 citations

Crystal structure of cleaved human α1-antichymotrypsin at 2.7 å resolution and its comparison with other serpins

1991 • 166 citations

Crystal structure of plakalbumin, a proteolytically nicked form of ovalbumin

1990 • 164 citations

Three-dimensional structure of porcine procarboxypeptidase B: a structural basis of its inactivity.

1991 • 155 citations

Solution structure of recombinant hirudin and the Lys-47 .fwdarw. Glu mutants: a nuclear magnetic resonance and hybrid distance geometry-dynamical simulated annealing study

1989 • 155 citations

The inactivation of human plasma alpha 1-proteinase inhibitor by proteinases from Staphylococcus aureus.

1986 • 151 citations

Substrate properties of C1 inhibitor Ma (alanine 434—-glutamic acid). Genetic and structural evidence suggesting that the P12-region contains critical determinants of serine protease inhibitor/substrate status

1991 • 147 citations

Refined 1.2 A crystal structure of the complex formed between subtilisin Carlsberg and the inhibitor eglin c. Molecular structure of eglin and its detailed interaction with subtilisin.

1986 • 147 citations

Crystallographic refinement of Japanese quail ovomucoid, a Kazal-type inhibitor, and model building studies of complexes with serine proteases

1982 • 142 citations

Crystal structure of a Kunitz-type trypsin inhibitor from Erythrina coffra seeds

1991 • 129 citations

Conformation of recombinant desulfatohirudin in aqueous solution determined by nuclear magnetic resonance

1989 • 126 citations

The crystal and molecular structure of the third domain of silver pheasant ovomucoid (OMSVP3)

1985 • 119 citations

Crystal and molecular structure of chymotrypsin inhibitor 2 from barley seeds in complex with subtilisin Novo

1985 • 119 citations

Quantitative evaluation of the contribution of ionic interactions to the formation of the thrombin-hirudin complex

1989 • 117 citations

Evidence for a tetrahedral intermediate complex during serpin-proteinase interactions

1991 • 113 citations

Mutational analysis of a protein-folding pathway

1989 • 111 citations

X-ray crystal structure of the protease inhibitor domain of Alzheimer's amyloid .beta.-protein precursor

1990 • 110 citations

The Complete Covalent Structure of Hirudin. Localization of the Disulfide Bonds

1985 • 104 citations

Molecular dynamics refinement of a thermitase-eglin-c complex at 1.98 Å resolution and comparison of two crystal forms that differ in calcium content

1989 • 98 citations

Crystal structure of bovine trypsinogen at 1.8 Å resolution. I. Data collection, application of Patterson search techniques and preliminary structural interpretation

1976 • 98 citations

Serpin-serine protease binding kinetics: .alpha.2-antiplasmin as a model inhibitor

1991 • 93 citations

Structural effects induced by removal of a disulfide-bridge: the X-ray structure of the C30A/C51A mutant of basic pancreatic trypsin inhibitor at 1.6 Å

1990 • 92 citations

The refined 2.2‐Å (0.22‐nm) X‐ray crystal structure of the ternary complex formed by bovine trypsinogen, valine‐valine and the Arg¹⁵ analogue of bovine pancreatic trypsin inhibitor

1984 • 89 citations

Ligand binding: proteinase-protein inhibitor interactions

1991 • 89 citations

Biosynthesis of Membrane Polypeptides of Rat Liver Peroxisomes1

1987 • 87 citations

Structure of the complex of Streptomyces griseus proteinase B and polypeptide chymotrypsin inhibitor-1 from Russet Burbank potato tubers at 2.1 Å resolution

1989 • 84 citations

Crystal structure of a bacterial protein proteinase inhibitor (Streptomyces subtilisin inhibitor) at 2·6 Å resolution

1979 • 83 citations

Basic pancreatic trypsin inhibitor has unusual thermodynamic stability parameters

1983 • 82 citations

Crystal structure at 2.6 Å resolution of the complex of subtilisin BPN′ with Streptomyces subtilisin inhibitor

1984 • 82 citations

Recombinant chymotrypsin inhibitor 2: expression, kinetic analysis of inhibition with .alpha.-chymotrypsin and wild-type and mutant subtilisin BPN', and protein engineering to investigate inhibitory specificity and mechanism

1990 • 79 citations

Crystallization, crystal structure analysis and molecular model of the third domain of Japanese quail ovomucoid, a Kazal type inhibitor

1981 • 75 citations

Refined crystal structures of subtilisin Novo in complex with wild-type and two mutant eglins

1991 • 75 citations

Structure of the Trypsin-Binding Domain of Bowman-Birk Type Protease Inhibitor and Its Interaction with Trypsin1

1986 • 73 citations

The amino acid sequence of a novel inhibitor of cathepsin D from potato

1990 • 73 citations

The structure of the complex formed by bovine trypsin and bovine pancreatic trypsin inhibitor

1975 • 72 citations

Use of fragments of hirudin to investigate thrombin‐hirudin interaction

1990 • 72 citations

Turkey ovomucoid third domain inhibits eight different serine proteinases of varied specificity on the same ...Leu18-Glu19... reactive site

1985 • 70 citations

Alpha-2-Antiplasmin: a Serpin with Two Separate But Overlapping Reactive Sites

1988 • 69 citations

Comparison of the solution and X-ray structures of barley serine proteinase inhibitor 2

1987 • 64 citations

Modeling the intact form of the α₁-proteinase inhibitor

1990 • 63 citations

Kinetics and Thermodynamics of the Interaction of Proteinases with Protein Inhibitors

1974 • 62 citations

Use of restrained molecular dynamics in water to determine three‐dimensional protein structure: Prediction of the three‐dimensional structure of Ecballium elaterium trypsin inhibitor II

1989 • 62 citations

Crystal structure of a Y35G mutant of bovine pancreatic trypsin inhibitor

1991 • 61 citations

Amino acid sequences of ovomucoid third domains from 27 additional species of birds

1993 • 61 citations

The S variant of human α₁-antitrypsin, structure and implications for function and metabolism

1989 • 59 citations

Three-dimensional structure of the complexes between bovine chymotrypsinogen A and two recombinant variants of human pancreatic secretory trypsin inhibitor (Kazal-type)

1991 • 56 citations

Stopped-flow kinetics of the resynthesis of the reactive site peptide bond in kallikrein inhibitor (Kunitz) by β-trypsin

1978 • 56 citations

Molecular recognition at the active site of subtilisin BPN': crystallographic studies using genetically engineered proteinaceous inhibitor SSI (Streptomyces subtilisin inhibitor)

1991 • 54 citations

Effect of single amino acid replacements on the thermodynamics of the reactive site peptide bond hydrolysis in ovomucoid third domain

1991 • 54 citations

Analysis of the activation process of porcine procarboxypeptidase B and determination of the sequence of its activation segment

1991 • 54 citations

The three‐dimensional structure of the bifunctional proteinase k/α‐amylase inhibitor from wheat (PKI3) at 2.5 Å resolution

1991 • 53 citations

Inactivation of human plasma serine proteinase inhibitors (serpins) by limited proteolysis of the reactive site loop with snake venom and bacterial metalloproteinases

1986 • 53 citations

Crystallographic refinement by incorporation of molecular dynamics: thermostable serine protease thermitase complexed with eglin c

1989 • 52 citations

[2] Two-dimensional nuclear magnetic resonance spectroscopy of proteins: An overview

1989 • 52 citations

Stability studies on derivatives of the bovine pancreatic trypsin inhibitor

1987 • 51 citations

Nuclear magnetic resonance solution and X-ray structures of squash trypsin inhibitor exhibit the same conformation of the proteinase binding loop

1989 • 50 citations

Primary structure of the major pepsin inhibitor from the intestinal parasitic nematode Ascaris suum

1990 • 49 citations

Dissecting the roles of individual interactions in protein stability: Lessons from a circularized protein

1985 • 49 citations

Inhibitory activity and conformational transition of α₁‐proteinase inhibitor variants

1991 • 49 citations

Proton assignments and secondary structure determination of the soybean trypsin/chymotrypsin Bowman-Birk inhibitor

1991 • 48 citations

Role of arginine 67 in the stabilization of chymotrypsin inhibitor 2: examination of amide proton exchange rates and denaturation thermodynamics of an engineered protein

1990 • 44 citations

The NMR structure of the activation domain isolated from porcine procarboxypeptidase B.

1991 • 43 citations

The tryptic activation pathway of monomeric procarboxypeptidase A.

1990 • 43 citations

Amino acid sequences of ovomucoid third domain from 25 additional species of birds

1990 • 41 citations

Interaction of chicken cystatin with inactivated papains

1989 • 40 citations

Dermasterias imbricata trypsin 1: an enzyme which rapidly hydrolyzes the reactive-site peptide bonds of protein trypsin inhibitors

1980 • 37 citations

Preparation of chemically ‘mutated’ aprotinin homologues by semisynthesis

1988 • 35 citations

Refined X-ray crystal structures of the reactive site modified ovomucoid inhibitor third domains from silver pheasant (OMSVP3∗) and from Japanese quail (OMJPQ3∗)

1991 • 34 citations

Sequential resonance assignment and secondary structure determination of the Ascaris trypsin inhibitor, a member of a novel class of proteinase inhibitors

1990 • 32 citations

Expression of the protease inhibitor ecotin and its co-crystallization with trypsin

1991 • 27 citations

Kinetics and mechanism for the conformational transition in p-guanidinobenzoate bovine trypsinogen induced by the isoleucine-valine dipeptide

1979 • 27 citations

An algorithmic approach to sequence → reactivity of proteins

1980 • 25 citations

Functional Properties of Peptides Derived from Seminalplasmin: Binding to Monospecific Anti-Seminalplasmin Immunoglobulins G and Calmodulin

1990 • 24 citations

Thermodynamics and kinetics of the hydrolysis and resynthesis of the reactive site peptide bond in turkey ovomucoid third domain by aspergillopeptidase B.

1983 • 18 citations

Two-dimensional NMR studies of Kazal proteinase inhibitors. 2. Sequence-specific assignments and secondary structure of reactive site modified turkey ovomucoid third domain

1988 • 18 citations

Intrinsic enzymic activity of bovine procarboxypeptidase A S₅

1974 • 17 citations

Synthesis of peptide fragments related to eglin c and examination of their inhibitory effect on human leukocyte elastase, cathepsin G and α-chymotrypsin

1989 • 13 citations

Crystallization and preliminary crystallographic study of cathepsin D inhibitor from potatoes

1991 • 11 citations

X‐ray crystal structure of the bovine α‐chymotrypsin/eglin c complex at 2.6 Å resolution

1990 • 11 citations

Interaction between Squash Inhibitors and Bovine Trypsinogen

1991 • 9 citations

The Three-dimensional Structure of Plasminostreptin, a Bacterial Protein Protease Inhibitor, at 2.8Å Resolution

1984 • 8 citations

Preliminary x‐ray analysis of crystals of plasminogen activator inhibitor‐1

1991 • 7 citations

Accuracy of cholesterol measurements

1991 • 1 citations