Refined structure of the Hirudin-thrombin complex

The interpretation of protein structures: Estimation of static accessibility

1971 • 5,771 citations

New Perspectives in Cell Adhesion: RGD and Integrins

1987 • 4,835 citations

Protein Inhibitors of Proteinases

1980 • 2,220 citations

HUMAN PLASMA PROTEINASE INHIBITORS

1983 • 2,129 citations

A graphics model building and refinement system for macromolecules

1978 • 1,858 citations

Traitement statistique des erreurs dans la determination des structures cristallines

1952 • 1,284 citations

The refined 1.9 A crystal structure of human alpha-thrombin: interaction with D-Phe-Pro-Arg chloromethylketone and significance of the Tyr-Pro-Pro-Trp insertion segment.

1989 • 889 citations

The detection of sub-units within the crystallographic asymmetric unit

1962 • 867 citations

The Structure of a Complex of Recombinant Hirudin and Human α-Thrombin

1990 • 718 citations

Structural basis of the activation and action of trypsin

1978 • 669 citations

Kinetics of the inhibition of thrombin by hirudin

1986 • 581 citations

Crystal structure of the thrombin-hirudin complex: a novel mode of serine protease inhibition.

1990 • 362 citations

Refinement of large structures by simultaneous minimization of energy and R factor

1978 • 354 citations

[69] Hirudin as an inhibitor of thrombin

1970 • 311 citations

Computing in Crystallography

1981 • 303 citations

Characterization of the complementary deoxyribonucleic acid and gene coding for human prothrombin

1983 • 299 citations

Proteinase inhibitors

1996 • 275 citations

Structure of the hirugen and hirulog 1 complexes of α-thrombin

1991 • 268 citations

Thrombin structure and function

1991 • 250 citations

The Reverse Turn as a Polypeptide Conformation in Globular Proteins

1973 • 249 citations

Incorporation of fast Fourier transforms to speed restrained least-squares refinement of protein structures

1987 • 221 citations

Regulation of Thrombin Generation and Functions

1988 • 212 citations

Thrombin specificity

1985 • 204 citations

THROMBIN SPECIFICITY*

1981 • 202 citations

Cloning and expression of a cDNA coding for the anticoagulant hirudin from the bloodsucking leech, Hirudo medicinalis.

1986 • 198 citations

Thrombin active site regions required for fibroblast receptor binding and initiation of cell division.

1980 • 186 citations

Thrombin^a

1986 • 186 citations

Platelet glycoprotein IIb-IIIa-like proteins mediate endothelial cell attachment to adhesive proteins and the extracellular matrix.

1987 • 185 citations

The refined 2.0 Å X‐ray crystal structure of the complex formed between bovine β‐trypsin and CMTI‐I, a trypsin inhibitor from squash seeds (Cucurbita maxima) Topological similarity of the squash seed inhibitors with the carboxypeptidase A inhibitor from potatoes

1989 • 181 citations

Use of site-directed mutagenesis to investigate the basis for the specificity of hirudin

1988 • 170 citations

Primary structure of human prethrombin 2 and alpha-thrombin.

1977 • 168 citations

The chemistry and physiology of human plasma proteins

1980 • 167 citations

Chemistry and Biology of Thrombin

1978 • 166 citations

Polymers of tripeptides as collagen models

1969 • 156 citations

Solution structure of recombinant hirudin and the Lys-47 .fwdarw. Glu mutants: a nuclear magnetic resonance and hybrid distance geometry-dynamical simulated annealing study

1989 • 155 citations

The functional domain of hirudin, a thrombin‐specific inhibitor

1983 • 151 citations

Crystal Structure Analysis and Molecular Model of Human C3a Anaphylatoxin

1980 • 133 citations

Conformation of recombinant desulfatohirudin in aqueous solution determined by nuclear magnetic resonance

1989 • 126 citations

Interaction of hirudin with thrombin: identification of a minimal binding domain of hirudin that inhibits clotting activity

1988 • 124 citations

Identification of regions of .alpha.-thrombin involved in its interaction with hirudin

1987 • 122 citations

Die automatisierte Faltmolekülmethode

1965 • 111 citations

Antithrombin properties of C‐terminus of hirudin using synthetic unsulfated N^α‐acetyl‐hirudin_45–65

1987 • 108 citations

The Complete Covalent Structure of Hirudin. Localization of the Disulfide Bonds

1985 • 104 citations

Contribution of the N-terminal region of hirudin to its interaction with thrombin

1989 • 91 citations

The assembly of blood clotting complexes on membranes

1987 • 91 citations

Structural and functional properties of human α-thrombin, phosphopyridoxylated α-thrombin, and γT-thrombin

1989 • 90 citations

Interaction of site specific hirudin variants with α-thrombin

1988 • 89 citations

Structure of prothrombin fragment 1 refined at 2.8 Å resolution

1988 • 85 citations

Thrombin Active-Site Regions

1986 • 85 citations

Primary structures of new ‘iso‐hirudins’

1989 • 82 citations

Covalent structures of beta and gamma autolytic derivatives of human alpha-thrombin.

1984 • 82 citations

Thrombin-bound conformation of the C-terminal fragments of hirudin determined by transferred nuclear Overhauser effects

1990 • 79 citations

Proflavine binding within the fibrinopeptide groove adjacent to the catalytic site of human .alpha.-thrombin

1984 • 74 citations

Expression and Secretion in S. Cerevisiae of Biologically Active Leech Hirudin

1988 • 74 citations

Physical evidence for an apolar binding site near the catalytic center of human α-thrombin

1977 • 73 citations

Chemical Synthesis and Expression of a Gene Coding for Hirudin, the Thrombin-Specific Inhibitor from the LeechHirudo medicinalis

1986 • 69 citations

Human thrombin: Partial primary structure

1977 • 57 citations

Anticoagulant peptides. Nature of the interaction of the C-terminal region of hirudin with a noncatalytic binding site on thrombin

1987 • 56 citations

The hirudin-binding site of human α-thrombin

1989 • 53 citations

Analysis of the secondary structure of hirudin and the mechanism of its interaction with thrombin

1988 • 52 citations

Staphylocoagulase-Binding Region in Human Prothrombin12

1985 • 52 citations

Biochemistry and Genetic Engineering of Hirudin

1989 • 51 citations

Human .alpha.- to .zeta.-thrombin cleavage occurs with neutrophil cathepsin G or chymotrypsin while fibrinogen clotting activity is retained

1990 • 48 citations

A group refinement procedure in protein crystallography using Fourier transforms

1985 • 47 citations

Use of the rotation and translation functions

1985 • 44 citations

The structural elements of hirudin which bind to the fibrinogen recognition site of thrombin are exclusively located within its acidic C‐terminal tail

1990 • 40 citations

Point mutations modifying the thrombin inhibition kinetics and antithrombotic activity in vivo of recombinant hirudin

1989 • 39 citations

Structure of the hirugen and hirulog 1 complexes of α-thrombin

1991 • 32 citations

The Mechanism of Association of Trypsin (or Chymotrypsin) with the Pancreatic Trypsin Inhibitors (Kunitz and Kazal) Kinetics and Thermodynamics of the Interaction

1974 • 28 citations

Interaction of Thrombin with Thrombomodulin

1986 • 24 citations

X-Ray Crystal Structures of Human α-Thrombin and of the Human Thrombin-Hirudin Complex

1992 • 14 citations

THE RELATION OF THE DIET TO THE COMPOSITION OF TISSUE PHOSPHOLIPIDES

1946 • 14 citations