Nuclear protein import is decreased by engineered mutants of nuclear transport factor 2 (NTF2) that do not bind GDP-Ran

The CCP4 suite: programs for protein crystallography

1994 • 17,203 citations

Improved methods for building protein models in electron density maps and the location of errors in these models

1991 • 12,831 citations

Methods in yeast genetics

1979 • 7,824 citations

Methods in Yeast Genetics

1988 • 4,905 citations

Nuclear targeting sequences — a consensus?

1991 • 1,908 citations

Improved Fourier coefficients for maps using phases from partial structures with errors

1986 • 1,897 citations

Cdi1, a human G1 and S phase protein phosphatase that associates with Cdk2

1993 • 1,531 citations

[10] 5-Fluoroorotic acid as a selective agent in yeast molecular genetics

1987 • 1,375 citations

A Small Ubiquitin-Related Polypeptide Involved in Targeting RanGAP1 to Nuclear Pore Complex Protein RanBP2

1997 • 1,221 citations

A novel ubiquitin-like modification modulates the partitioning of the Ran-GTPase-activating protein RanGAP1 between the cytosol and the nuclear pore complex.

1996 • 1,161 citations

Nucleocytoplasmic Transport

1996 • 1,071 citations

Nucleocytoplasmic transport: signals, mechanisms and regulation

1997 • 987 citations

Nuclear protein import in permeabilized mammalian cells requires soluble cytoplasmic factors.

1990 • 909 citations

Protein import into nuclei: association and dissociation reactions involving transport substrate, transport factors, and nucleoporins

1995 • 767 citations

The GTP-binding protein Ran/TC4 is required for protein import into the nucleus

1993 • 760 citations

Isolation of a protein that is essential for the first step of nuclear protein import

1994 • 710 citations

Catalysis of guanine nucleotide exchange on Ran by the mitotic regulator RCC1

1991 • 703 citations

The two-hybrid system: an assay for protein-protein interactions

1994 • 649 citations

Identification of different roles for RanGDP and RanGTP in nuclear protein import.

1996 • 644 citations

Inhibition of nuclear protein import by nonhydrolyzable analogues of GTP and identification of the small GTPase Ran/TC4 as an essential transport factor [published erratum appears in J Cell Biol 1994 Jan;124(1-2):217]

1993 • 544 citations

Movement of a karyophilic protein through the nuclear pores of oocytes.

1984 • 510 citations

Distinct functions for the two importin subunits in nuclear protein import

1995 • 479 citations

The peptide repeat domain of nucleoporin Nup98 functions as a docking site in transport across the nuclear pore complex

1995 • 460 citations

Identification of NTF2, a cytosolic factor for nuclear import that interacts with nuclear pore complex protein p62.

1995 • 385 citations

The RCC1 protein, a regulator for the onset of chromosome condensation locates in the nucleus and binds to DNA.

1989 • 367 citations

Identification of hSRP1 alpha as a functional receptor for nuclear localization sequences

1995 • 337 citations

Purification of a Ran-interacting protein that is required for protein import into the nucleus.

1994 • 318 citations

Nuclear protein import

1997 • 296 citations

Pores for thought: nuclear pore complex proteins

1994 • 286 citations

Previously identified protein of uncertain function is karyopherin alpha and together with karyopherin beta docks import substrate at nuclear pore complexes.

1995 • 254 citations

The conserved amino-terminal domain of hSRP1 alpha is essential for nuclear protein import.

1996 • 252 citations

Human RanGTPase-activating protein RanGAP1 is a homologue of yeast Rna1p involved in mRNA processing and transport.

1995 • 247 citations

The yeast RNA1 gene product necessary for RNA processing is located in the cytosol and apparently excluded from the nucleus.

1990 • 228 citations

Ras/Rap effector specificity determined by charge reversal

1996 • 214 citations

A GTPase Controlling Nuclear Trafficking: Running the Right Way or Walking RANdomly?

1996 • 211 citations

Nucleocytoplasmic transport of macromolecules

1997 • 191 citations

Crystal structure of the nuclear Ras-related protein Ran in its GDP-bound form

1995 • 188 citations

Molecular and functional characterization of the p62 complex, an assembly of nuclear pore complex glycoproteins.

1996 • 177 citations

RanBP1 stabilizes the interaction of Ran with p97 nuclear protein import.

1996 • 175 citations

Functional interaction of Nic96p with a core nucleoporin complex consisting of Nsp1p, Nup49p and a novel protein Nup57p.

1995 • 167 citations

Role of the Nuclear Transport Factor p10 in Nuclear Import

1996 • 166 citations

Interaction Trap/Two‐Hybrid System to Identify Interacting Proteins

2011 • 156 citations

The 1.6 Å Resolution Crystal Structure of Nuclear Transport Factor 2 (NTF2)

1996 • 145 citations

GTP hydrolysis by Ran occurs at the nuclear pore complex in an early step of protein import.

1995 • 139 citations

PCR protocols: current methods and applications.

1993 • 129 citations

Human RanGTPase activating protein RanGAP1 is a homolog of yeast RNA1p involved in messenger RNA processing and transport

1995 • 125 citations

Separate Binding Sites on Nuclear Transport Factor 2 (NTF2) for GDP-Ran and the Phenylalanine-rich Repeat Regions of Nucleoporins p62 and Nsp1p

1996 • 122 citations

Toward the molecular dissection of protein import into nuclei

1996 • 112 citations

The small nuclear GTPase Ran: How much does it run?

1996 • 112 citations

Nuclear protein import: Ran-GTP dissociates the karyopherin alphabeta heterodimer by displacing alpha from an overlapping binding site on beta.

1996 • 105 citations

The NTF2 Gene Encodes an Essential, Highly Conserved Protein That Functions in Nuclear Transport in Vivo

1996 • 102 citations

Site-directed mutagenesis by double polymerase chain reaction

1995 • 100 citations

Interaction between the Small GTPase Ran/Gsp1p and Ntf2p Is Required for Nuclear Transport

1997 • 98 citations

A Role for Nucleoporin FG Repeat Domains in Export of Human Immunodeficiency Virus Type 1 Rev Protein and RNA from the Nucleus

1996 • 90 citations

Ran, a GTPase involved in nuclear processes: its regulators and effectors

1996 • 81 citations

Nucleotide-specific interaction of Ran/TC4 with nuclear transport factors NTF2 and p97.

1996 • 80 citations

How does the switch II region of G‐domains work?

1993 • 80 citations

Nucleocytoplasmic transport of macromolecules.

1997 • 76 citations

Localization of the Ran-GTP binding protein RanBP2 at the cytoplasmic side of the nuclear pore complex.

1995 • 70 citations

Site-Directed Mutagenesis by Double Polymerase Chain Reaction: Megaprimer Method

2003 • 70 citations

Macromolecular interactions in the nucleoporin p62 complex of rat nuclear pores: binding of nucleoporin p54 to the rod domain of p62.

1995 • 57 citations

Exogenously injected nuclear import factor p10/NTF2 inhibits signal‐mediated nuclear import and export of proteins in living cells

1996 • 48 citations

DNA cloning 2: Expression systems. A practical approach

1996 • 45 citations

Interaction Trap/Two‐Hybrid System to Identify Interacting Proteins

2000 • 33 citations

Crystallization and Preliminary X-Ray Diffraction Analysis of Nuclear Transport Factor 2

1996 • 28 citations

Expression in yeast of binding regions of karyopherins α and β inhibits nuclear import and cell growth

1996 • 20 citations

Nuclear pores and macromolecular assemblies involved in nucleocytoplasmic transport

1996 • 11 citations