Expression of the pspA gene stimulates efficient protein export in Escherichia coli

A rapid alkaline extraction procedure for screening recombinant plasmid DNA

1979 • 15,511 citations

Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid

1978 • 4,472 citations

Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and Mu

1976 • 1,895 citations

Electrophoretic resolution of the ‘major outer membrane protein’ of Escherichia coli K12 into four bands

1975 • 1,439 citations

The signal peptide

1990 • 1,096 citations

The binding cascade of SecB to SecA to SecYE mediates preprotein targeting to the E. coli plasma membrane

1990 • 561 citations

The purified E. coli integral membrane protein is sufficient for reconstitution of SecA-dependent precursor protein translocation

1990 • 516 citations

E. coli mutant pleiotropically defective in the export of secreted proteins

1981 • 512 citations

GENETIC ANALYSIS OF PROTEIN EXPORT IN ESCHERICHIA COLI K12

1985 • 432 citations

SecA protein hydrolyzes ATP and is an essential component of the protein translocation ATPase of Escherichia coli.

1989 • 406 citations

Copper staining: A five-minute protein stain for sodium dodecyl sulfate-polyacrylamide gels

1987 • 405 citations

Protein localization in E. coli: Is there a common step in the secretion of periplasmic and outer-membrane proteins?

1981 • 359 citations

Regulation of a membrane component required for protein secretion in escherichia coli

1982 • 356 citations

GENETIC ANALYSIS OF PROTEIN EXPORT IN ESCHERICHIA COLI

1990 • 291 citations

Evidence for specificity at an early step in protein export in Escherichia coli

1985 • 272 citations

Use of gene fusion to study secretion of maltose-binding protein into Escherichia coli periplasm

1979 • 267 citations

Phosphatidylglycerol is involved in protein translocation across Escherichia coli inner membranes

1988 • 263 citations

Phage shock protein, a stress protein of Escherichia coli.

1990 • 255 citations

A defined mutation in the protein export gene within the spc ribosomal protein operon of Escherichia coli: isolation and characterization of a new temperature-sensitive secY mutant.

1984 • 216 citations

Outer Membrane Proteins of Escherichia coli III. Evidence that the Major Protein of Escherichia coli O111 Outer Membrane Consists of Four Distinct Polypeptide Species

1974 • 196 citations

Characterization of membrane-associated and soluble states of SecA protein from wild-type and SecA51(TS) mutant strains of Escherichia coli.

1991 • 196 citations

SecA insertion into phospholipids is stimulated by negatively charged lipids and inhibited by ATP: a monolayer study

1992 • 175 citations

Outer membrane protein e of Escherichia coli K-12 is co-regulated with alkaline phosphatase

1980 • 167 citations

Negatively charged phospholipids restore prePhoE translocation across phosphatidylglycerol-depleted Escherichia coli inner membranes

1991 • 159 citations

The ultimate localization of an outer membrane protein of Escherichia coli K-12 is not determined by the signal sequence.

1983 • 151 citations

Characterization and sequence of the Escherichia coli stress-induced psp operon

1991 • 147 citations

Stress-induced expression of the Escherichia coli phage shock protein operon is dependent on sigma 54 and modulated by positive and negative feedback mechanisms.

1991 • 135 citations

PrlA (SecY) and PrlG (SecE) interact directly and function sequentially during protein translocation in E. coli

1990 • 129 citations

Localization and processing of outer membrane and periplasmic proteins in Escherichia coli strains harboring export-specific suppressor mutations.

1982 • 124 citations

Escherichia coli SecB protein associates with exported protein precursors in vivo.

1989 • 123 citations

Periplasmic accumulation of truncated forms of outer-membrane PhoE protein of Escherichia coli K-12

1986 • 121 citations

Optimal posttranslational translocation of the precursor of PhoE protein across Escherichia coli membrane vesicles requires both ATP and the protonmotive force

1987 • 120 citations

Expression of outer membrane protein e of escherichia coli K12 by phosphate limitation

1980 • 118 citations

Failure of E. coli K-12 to transport PhoE-LacZ hybrid proteins out of the cytoplasm.

1985 • 117 citations

Precursor protein translocation by the Escherichia coli translocase is directed by the protonmotive force.

1992 • 113 citations

Synthesis of a precursor to the B subunit of heat-labile enterotoxin in Escherichia coli

1981 • 100 citations

Secretion and membrane integration of a filamentous phage-encoded morphogenetic protein

1990 • 84 citations

Sequence information within the lamB gene is required for proper routing of the bacteriophage λ receptor protein to the outer membrane of Escherichia coli K-12

1982 • 79 citations

Outer-membrane PhoE protein of Escherichia coli K-12 as an exposure vector: possibilities and limitations

1990 • 74 citations

Cell surface of the fish pathogenic bacterium Aeromonas salmonicida

1982 • 63 citations

Regulation of the Escherichia coli secA gene by protein secretion defects: analysis of secA, secB, secD, and secY mutants

1988 • 56 citations

The role of the carboxy-terminal membrane-spanning fragment in the biogenesis of Escherichia coli K12 outer membrane protein PhoE

1989 • 52 citations

The folding properties of the Escherichia coli maltose-binding protein influence its interaction with SecB in vitro

1990 • 46 citations

Biogenesis of outer membrane protein PhoE of Escherichia coli

1992 • 45 citations

The role of the positively charged N-terminus of the signal sequence of E. coli outer membrane protein PhoE in export

1989 • 44 citations

Export and localization of N-terminally truncated derivatives of Escherichia coli K-12 outer membrane protein PhoE.

1988 • 40 citations

Export and processing of MalE-LacZ hybrid proteins in Escherichia coli

1984 • 35 citations

SecB‐binding does not maintain the translocation‐competent state of prePhoE

1992 • 31 citations

Translocation of nascent non-signal sequence protein in heated Escherichia coli.

1986 • 28 citations

In vitro trimerization of outer membrane protein PhoE

1990 • 28 citations

Expression ofEscherichia coliPhoE protein in avirulentSalmonella typhimurium aroAandgalEstrains

1988 • 28 citations

Effects of linker insertions on the biogenesis and functioning of the Escherichia coli outer membrane pore protein PhoE

1987 • 27 citations

Conservation of components of theEscherichia coliexport machinery in prokaryotes

1991 • 26 citations

Role of the cell surface‐exposed regions of outer membrane protein PhoE of Escherichia coli K12 in the biogenesis of the protein

1989 • 25 citations

Efficient recognition by rat T cell clones of an epitope of mycobacterial hsp 65 inserted in Escherichia coli outer membrane protein PhoE

1990 • 23 citations

Temperature-sensitive sec mutants of Escherichia coli: inhibition of protein export at the permissive temperature

1989 • 21 citations

Subcellular localization of a PhoE‐LacZ fusion protein in E. coli by protease accessibility experiments reveals an inner‐membrane‐spanning form of the protein

1987 • 14 citations

Conservation of components of the export machinery in prokaryotes

1991 • 7 citations