Functional expression of mammalian cytochromes P450IIB in the yeast Saccharomyces cerevisiae

PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT

1951 • 319,299 citations

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

Methods in Enzymology , Vol

1966 • 26,261 citations

Studies on transformation of Escherichia coli with plasmids

1983 • 11,179 citations

DNA cloning : a practical approach

1985 • 2,302 citations

The P450 Superfamily: Update on New Sequences, Gene Mapping, and Recommended Nomenclature

1991 • 1,092 citations

An improved assay of 7-ethoxycoumarin O-deethylase activity: induction of hepatic enzyme activity in C57BL/6J and DBA/2J mice by phenobarbital, 3-methylcholanthrene and 2,3,7,8-tetrachlorodibenzo-p-dioxin.

1978 • 618 citations

Properties of electrophoretically homogeneous phenobarbital-inducible and beta-naphthoflavone-inducible forms of liver microsomal cytochrome P-450

1976 • 536 citations

Purified liver microsomal cytochrome P-450. Separation and characterization of multiple forms.

1975 • 456 citations

Expression of Rat Liver Cytochrome P-450MC cDNA inSaccharomyces cerevisiae

1985 • 221 citations

Experimental Manipulation of Gene Expression

1983 • 213 citations

Microsomes, drug oxidations, and drug toxicity

1982 • 187 citations

Galactose as a gratuitous inducer of GAL gene expression in yeasts growing on glucose

1989 • 168 citations

Expression of a human liver cytochrome P-450 protein with tolbutamide hydroxylase activity in Saccharomyces cerevisiae

1989 • 134 citations

The rabbit pulmonary monooxygenase system. Immunochemical and biochemical characterization of enzyme components.

1979 • 115 citations

SPECIFICITY, KINETICS, AND INHIBITION OF α- AND β-HYDROXYSTEROID DEHYDROGENASES

1956 • 111 citations

Maximizing the expression of mammalian cytochrome P-450 monooxygenase activities in yeast cells

1990 • 110 citations

Amino-terminal sequence of phenobarbital-inducible cytochrome P-450 from rabbit liver microsomes: Similarity to hydrophobic amino-terminal segments of preproteins

1977 • 104 citations

cDNA cloning and functional expression in yeast Saccharomyces cerevisiae of β‐naphthoflavone‐induced rabbit liver P‐450 LM4 and LM6

1988 • 101 citations

Secondary structure determination for α‐neurotoxin from Dendroaspis polylepis polylepis based on sequence‐specific ¹H‐nuclear‐magnetic‐resonance assignments

1988 • 86 citations

Purification and characterization of the dog hepatic cytochrome P-450 isozyme responsible for the metabolism of 2,2′,4,4′,5,5′-hexachlorobiphenyl

1987 • 83 citations

Novel exogenous heme-dependent expression of mammalian cytochrome P450 using baculovirus

1989 • 83 citations

Human cDNA‐Expressed Cytochrome P450 IA2: Mutagen Activation and Substrate Specificity

1989 • 72 citations

Analysis of two benzo[a]pyrene-resistant mutants of the mouse hepatoma Hepa-1 P(1)450 gene via cDNA expression in yeast.

1987 • 68 citations

On the mechanism of the inactivation of the major phenobarbital-inducible isozyme of rat liver cytochrome P-450 by chloramphenicol.

1985 • 64 citations

Expression of Human Placental Aromatase inSaccharomyces cerevisiae

1989 • 55 citations

Constitutive and inducible expression of human cytochrome P450IA1 in yeast Saccharomyces cerevisiae: An alternative enzyme source for in vitro studies

1990 • 52 citations

Reconstitution of the rabbit pulmonary microsomal mixed-function oxidase system from solubilized components.

1975 • 51 citations

cDNA and deduced amino acid sequences of a dog hepatic cytochrome P450IIB responsible for the metabolism of 2,2′,4,4′,5,5′-hexachlorobiphenyl

1990 • 49 citations

Reconstitution of the liver microsomal hydroxylase system into liposomes

1977 • 48 citations

Catalytic properties of purified forms of rabbit liver microsomal cytochrome P-450 in reconstituted phospholipid vesicles

1980 • 47 citations

Expression of a human P-450IIC gene in yeast cells using galactose-inducible expression system.

1989 • 46 citations

Characterization of Rabbit Liver Cytochrome P-450 (Laurate ω-1 Hydroxylase) Synthesized in Transformed Yeast Cells1

1988 • 38 citations

Pronounced and differential effects of ionic strength and pH on testosterone oxidation by membrane-bound and purified forms of rat liver microsomal cytochrome P-450

1990 • 37 citations

Analogues of chloramphenicol as mechanism-based inactivators of rat liver cytochrome P-450: modifications of the propanediol side chain, the p-nitro group, and the dichloromethyl moiety.

1986 • 33 citations

Expression of Alcohol-Inducible Rabbit Liver Cytochrome P-450 3a (P-450IIE1) inSaccharomyces cerevisiaewith the Copper-Inducible CUP1 Promoter

1990 • 25 citations

The metabolism of xenobiotics and endogenous compounds by the constitutive dog liver cytochrome P450 PBD-2

1988 • 25 citations

Microheterogeneity in the major phenobarbital-inducible forms of rabbit liver microsomal cytochrome P-450 as revealed by nucleotide sequencing of cloned cDNAs

1988 • 25 citations

The expression of cytochrome P450IIB1 in Saccharomyces cerevisia results in an increased mutation frequency when exposed to cyclophosphamide

1989 • 24 citations

Monooxygenase Activity of Saccharomyces cerevisiae Cells Transformed with Expression Plasmids Carrying Rat Cytochrome P-450MC cDNA1

1986 • 23 citations

Selective inactivation of four rat liver microsomal androstenedione hydroxylases by chloramphenicol analogs.

1988 • 21 citations

Expression and characterization of a rabbit liver cytochrome P450 belonging to P450IIB subfamily with the aid of the baculovirus expression vector system

1991 • 21 citations

Expression of a Rat Liver Microsomal Cytochrome P-450 Catalyzing Testosterone 16α-Hydroxylation in Saccharomyces cerevisiae: Vitamin D3 25-Hydroxylase and Testosterone 16α-Hydroxylase Are Distinct Forms of Cytochrome P-450

1988 • 17 citations

Characterization of Rabbit Liver P450IIE1 Synthesized in Transformed Yeast Cells1

1990 • 12 citations

Rat Liver Cytochrome P450IA2 Synthesized by Transfected COS-1 Cells Efficiently Activates Food-Derived Promutagens

1991 • 9 citations

Selective inactivation by 21-chlorinated steroids of rabbit liver and adrenal microsomal cytochromes P-450 involved in progesterone hydroxylation

1988 • 7 citations