Proteome research: Complementarity and limitations with respect to the RNA and DNA worlds

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

High resolution two-dimensional electrophoresis of proteins.

1975 • 19,515 citations

Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa

1987 • 11,491 citations

Mass Spectrometric Sequencing of Proteins from Silver-Stained Polyacrylamide Gels

1996 • 8,994 citations

Escherichia coli and Salmonella :cellular and molecular biology

1996 • 8,124 citations

Principal Component Analysis

1986 • 7,864 citations

An approach to correlate tandem mass spectral data of peptides with amino acid sequences in a protein database

1994 • 6,412 citations

Whole-Genome Random Sequencing and Assembly of Haemophilus influenzae Rd

1995 • 5,571 citations

Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes.

1987 • 5,066 citations

Life with 6000 Genes

1996 • 4,125 citations

High resolution two-dimensional electrophoresis of basic as well as acidic proteins

1977 • 3,556 citations

Assay of proteins in the presence of interfering materials

1976 • 3,217 citations

Solubilization of membranes by detergents

1975 • 2,909 citations

A Protein Sequenator

1967 • 2,826 citations

Expression monitoring by hybridization to high-density oligonucleotide arrays

1996 • 2,796 citations

The Minimal Gene Complement of Mycoplasma genitalium

1995 • 2,499 citations

Sequence Analysis of the Genome of the Unicellular Cyanobacterium Synechocystis sp. Strain PCC6803. II. Sequence Determination of the Entire Genome and Assignment of Potential Protein-coding Regions (Supplement)

1996 • 2,455 citations

Two‐dimensional electrophoresis. The current state of two‐dimensional electrophoresis with immobilized pH gradients

1988 • 2,431 citations

A gas-liquid solid phase peptide and protein sequenator.

1981 • 2,238 citations

What is life? : the physical aspect of the living cell

1944 • 2,068 citations

Sequence Analysis of the Genome of the Unicellular Cyanobacterium Synechocystis sp. Strain PCC6803. II. Sequence Determination of the Entire Genome and Assignment of Potential Protein-coding Regions

1996 • 2,046 citations

Complete Genome Sequence of the Methanogenic Archaeon, Methanococcus jannaschii

1996 • 2,043 citations

Stochastic mechanisms in gene expression

1997 • 1,860 citations

Analytical Properties of the Nanoelectrospray Ion Source

1996 • 1,816 citations

Femtomole sequencing of proteins from polyacrylamide gels by nano-electrospray mass spectrometry

1996 • 1,687 citations

Rapid identification of proteins by peptide-mass fingerprinting

1993 • 1,619 citations

Robustness in simple biochemical networks

1997 • 1,531 citations

Error-Tolerant Identification of Peptides in Sequence Databases by Peptide Sequence Tags

1994 • 1,475 citations

Linking genome and proteome by mass spectrometry: Large-scale identification of yeast proteins from two dimensional gels

1996 • 1,430 citations

Method for Determination of the Amino Acid Sequence in Peptides.

1950 • 1,360 citations

[12] Determination of total protein

1983 • 1,309 citations

Identifying proteins from two-dimensional gels by molecular mass searching of peptide fragments in protein sequence databases.

1993 • 1,301 citations

A comparison of selected mRNA and protein abundances in human liver

1997 • 1,274 citations

Method to Correlate Tandem Mass Spectra of Modified Peptides to Amino Acid Sequences in the Protein Database

1995 • 1,269 citations

In-gel digestion of proteins for internal sequence analysis after one- or two-dimensional gel electrophoresis

1992 • 1,259 citations

Reverse Transcriptase Motifs in the Catalytic Subunit of Telomerase

1997 • 1,252 citations

Progress with Proteome Projects: Why all Proteins Expressed by a Genome Should be Identified and How To Do It

1996 • 1,252 citations

Protein mapping by combined isoelectric focusing and electrophoresis of mouse tissues

1975 • 1,213 citations

Protein sequencing by tandem mass spectrometry.

1986 • 1,165 citations

Complete Sequence Analysis of the Genome of the Bacterium Mycoplasma Pneumoniae

1996 • 1,143 citations

Gel electrophoresis of proteins: a practical approach

1981 • 1,082 citations

The focusing positions of polypeptides in immobilized pH gradients can be predicted from their amino acid sequences

1993 • 969 citations

Isoelectric focusing in immobilized pH gradients: Principle, methodology and some applications

1982 • 963 citations

Use of mass spectrometric molecular weight information to identify proteins in sequence databases

1993 • 936 citations

Progress with gene‐product mapping of the Mollicutes: Mycoplasma genitalium

1995 • 934 citations

From Proteins to Proteomes: Large Scale Protein Identification by Two-Dimensional Electrophoresis and Arnino Acid Analysis

1996 • 837 citations

Internal amino acid sequence analysis of proteins separated by one- or two-dimensional gel electrophoresis after in situ protease digestion on nitrocellulose.

1987 • 819 citations

Protein-protein interactions: methods for detection and analysis

1995 • 812 citations

Initial assessment of human gene diversity and expression patterns based upon 83 million nucleotides of cDNA sequence.

1995 • 804 citations

Gel electrophoresis of proteins: A practical approach

1982 • 798 citations

Proteolytic enzymes; a practical approach

1991 • 786 citations

Improvement of an "In-Gel" Digestion Procedure for the Micropreparation of Internal Protein Fragments for Amino Acid Sequencing

1995 • 755 citations

Two‐dimensional electrophoresis of proteins: An updated protocol and implications for a functional analysis of the genome

1995 • 724 citations

Peptide Mass Maps: A Highly Informative Approach to Protein Identification

1993 • 678 citations

ISOLATION OF THE LAC REPRESSOR

1966 • 657 citations

Induction of proteins in response to low temperature in Escherichia coli

1987 • 599 citations

Protein Identification by Mass Profile Fingerprinting

1993 • 595 citations

Heat‐shock and general stress response in Bacillus subtilis

1996 • 586 citations

A new generation of information retrieval tools for biologists: the example of the ExPASy WWW server

1994 • 564 citations

Isoelectric Focusing: Theory, Methodology and Applications

1983 • 563 citations

The yeast genome project: what did we learn?

1996 • 540 citations

Reference points for comparisons of two‐dimensional maps of proteins from different human cell types defined in a pH scale where isoelectric points correlate with polypeptide compositions

1994 • 523 citations

Direct Analysis and Identification of Proteins in Mixtures by LC/MS/MS and Database Searching at the Low-Femtomole Level

1997 • 516 citations

Ribosomes as sensors of heat and cold shock in Escherichia coli.

1990 • 516 citations

Differential induction of heat shock, SOS, and oxidation stress regulons and accumulation of nucleotides in Escherichia coli

1987 • 492 citations

Improvement of the solubilization of proteins in two‐dimensional electrophoresis with immobilized pH gradients

1997 • 484 citations

Proteolytic Enzymes: A Practical Approach

1990 • 413 citations

Two-dimensional gel analysis of soluble proteins. Charaterization of guinea pig exocrine pancreatic proteins.

1975 • 394 citations

ESTablishing a human transcript map

1995 • 391 citations

A nonlinear wide‐range immobilized pH gradient for two‐dimensional electrophoresis and its definition in a relevant pH scale

1993 • 386 citations

Mass spectrometric approaches for the identification of gel‐separated proteins

1995 • 378 citations

Mining Genomes: Correlating Tandem Mass Spectra of Modified and Unmodified Peptides to Sequences in Nucleotide Databases

1995 • 373 citations

Levels of major proteins of Escherichia coli during growth at different temperatures

1979 • 372 citations

Analysis of the induction of general stress proteins of Bacillus subtilis

1994 • 371 citations

The role of mRNA and protein stability in gene expression

1989 • 370 citations

[16] Analyzing protein-protein interactions using two-hybrid system

1995 • 364 citations

Immobilized pH Gradients: Theory and Methodology

1990 • 363 citations

Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K‐12

1997 • 341 citations

The gene wars: science, politics, and the human genome

1994 • 332 citations

Salmonella acid shock proteins are required for the adaptive acid tolerance response

1991 • 322 citations

Peptide sequencing by matrix‐assisted laser‐desorption mass spectrometry

1992 • 306 citations

Development of polyacrylamide gels that improve the separation of proteins and their detection by silver staining

1988 • 305 citations

Protein-blotting on Polybrene-coated glass-fiber sheets. A basis for acid hydrolysis and gas-phase sequencing of picomole quantities of protein previously separated on sodium dodecyl sulfate/polyacrylamide gel

1985 • 288 citations

The coming Kuhnian revolution in biology

1997 • 282 citations

Mass spectrometric sequencing of linear peptides by product‐ion analysis in a reflectron time‐of‐flight mass spectrometer using matrix‐assisted laser desorption ionization

1993 • 269 citations

A comparison between low background silver diammine and silver nitrate protein stains

1992 • 266 citations

Analysis of the Escherichia coli Genome: DNA Sequence of the Region from 84.5 to 86.5 Minutes

1992 • 249 citations

Proteins induced by aerobiosis in Escherichia coli

1983 • 244 citations

Very alkaline immobilized pH gradients for two‐dimensional electrophoresis of ribosomal and nuclear proteins

1997 • 238 citations

Two-Dimensional Electrophoresis and Immunological Techniques

1987 • 235 citations

Amino Acid Analysis by Reverse-Phase High-Performance Liquid Chromatography: Improved Derivatization and Detection Conditions with 9-Fluorenylmethyl Chloroformate

1996 • 230 citations

The Quadrupole Ion Trap Mass Spectrometer—A Small Solution to a Big Challenge

1997 • 228 citations

Sequence tag identification of intact proteins by matching tanden mass spectral data against sequence data bases.

1996 • 221 citations

Electrospray mass spectrometry for protein characterization

1995 • 218 citations

Rapid mass spectrometric peptide sequencing and mass matching for characterization of human melanoma proteins isolated by two-dimensional PAGE.

1995 • 206 citations

Gel electrophoresis: Proteins

1994 • 201 citations

Internal Sequences from Proteins Digested in Polyacrylamide Gels

1995 • 201 citations

Silver-staining of proteins in polyacrylamide gels: a general overview

2009 • 200 citations

Induction of heat shock proteins and thermotolerance by ethanol in Saccharomycescerevisiae

1982 • 198 citations

[27] Gene identification using the yeast two-hybrid system

1996 • 195 citations

Global analysis of proteins synthesized during phosphorus restriction in Escherichia coli

1996 • 195 citations

Mass spectrometry in protein studies from genome to function

1997 • 193 citations

Identification of the proteins of the yeast U1 small nuclear ribonucleoprotein complex by mass spectrometry

1997 • 193 citations

Twenty years of two‐dimensional electrophoresis: Past, present and future

1996 • 193 citations

Micropreparative two‐dimensional electrophoresis allowing the separation of samples containing milligram amounts of proteins

1993 • 191 citations

Survival, stress resistance, and alterations in protein expression in the marine vibrio sp. strain S14 during starvation for different individual nutrients

1992 • 189 citations

Ribosomes exist in large excess over the apparent demand for protein synthesis during carbon starvation in marine Vibrio sp. strain CCUG 15956

1992 • 186 citations

Two-dimensional electrophoresis

1991 • 185 citations

Electrophoresis: theory, techniques and biochemical and clinical applications

1986 • 184 citations

Protein identification by solid phase microextraction—capillary zone electrophoresis—microelectrospray—tandem mass spectrometry

1996 • 184 citations

Protein sequencing; a practical approach

1990 • 183 citations

Protein-electroblotting and -microsequencing strategies in generating protein data bases from two-dimensional gels.

1989 • 180 citations

The acid tolerance response of Salmonella typhimurium involves transient synthesis of key acid shock proteins

1993 • 177 citations

A Sequence Property Approach to Searching Protein Databases

1995 • 176 citations

From Genome to Proteome: Looking at a Cell's Proteins

1995 • 174 citations

A response of protein synthesis to temperature shift in the yeast Saccharomyces cerevisiae.

1979 • 169 citations

Identifying the major proteome components of Haemophilus influenzae type‐strain NCTC 8143

1997 • 169 citations

The Human Protein Index.

1982 • 165 citations

The Human Protein Index.

1982 • 161 citations

Identification of proteins in polyacrylamide gels by mass spectrometric peptide mapping combined with database search

1994 • 161 citations

Isolation of 10 differentially expressed cDNAs in p53-induced apoptosis: activation of the vertebrate homologue of the drosophila seven in absentia gene.

1996 • 160 citations

Determination of amino acid compositions and NH2-terminal sequences of peptides electroblotted onto PVDF membranes from Tricine-Sodium dodecyl sulfate-polyacrylamide gel electrophoresis: Application to peptide mapping of human complement component C3

1989 • 160 citations

Resolution power of two‐dimensional electrophoresis and identification of proteins from gels

1996 • 158 citations

Global control in Salmonella typhimurium: two-dimensional electrophoretic analysis of starvation-, anaerobiosis-, and heat shock-inducible proteins

1986 • 158 citations

Analysis of proteins synthesized by Salmonella typhimurium during growth within a host macrophage

1993 • 156 citations

Proteins induced by anaerobiosis in Escherichia coli

1983 • 153 citations

The Heterogeneity of Mouse‐Chromatin Nonhistone Proteins as Evidenced by Two‐Dimensional Polyacrylamide‐Gel Electrophoresis and Ion‐Exchange Chromatography

1974 • 152 citations

Interlaboratory reproducibility of yeast protein patterns analyzed by immobilized pH gradient two‐dimensional gel electrophoresis

1995 • 152 citations

Does replication‐induced transcription regulate synthesis of the myriad low copy number proteins of Escherichia coli?

1995 • 150 citations

First steps from a two‐dimensional protein index towards a response‐regulation map for Bacillus subtilis

1997 • 147 citations

The gene‐protein database of Escherichia coli: Edition 5

1992 • 142 citations

Purification and characterization of a metallo-endoproteinase from mouse kidney

1981 • 140 citations

Proteome studies of Saccharomyces cerevisiae: Identification and characterization of abundant proteins

1997 • 140 citations

Federated two‐dimensional electrophoresis database: A simple means of publishing two‐dimensional electrophoresis data

1996 • 138 citations

Protein identification in DNA databases by peptide mass fingerprinting

1994 • 131 citations

Human liver protein map: A reference database established by microsequencing and gel comparison

1992 • 129 citations

Cross‐species identification of proteins separated by two‐dimensional gel electrophoresis using matrix‐assisted laser desorption ionisation/time‐of‐flight mass spectrometry and amino acid composition

1995 • 127 citations

Identification of human myocardial proteins separated by two‐dimensional electrophoresis using an effective sample preparation for mass spectrometry

1996 • 127 citations

Sample immobilization protocols for matrix‐asssisted laser‐desorption mass spectrometry

1992 • 125 citations

From Electrophoretically Separated Protein to Identification: Strategies for Sequence and Mass Analysis

1994 • 124 citations

Proteome analysis: from protein characterization to biological function

1997 • 123 citations

Plasma and red blood cell protein maps: Update 1993

1993 • 122 citations

Mass spectrometric analysis of blotted proteins after gel electrophoretic separation by matrix‐assisted laser desorption/ionization

1992 • 120 citations

Membrane protein analysis by isoelectric focusing in immobilized pH gradients

1985 • 118 citations

Developments in matrix-assisted laser desorption/ionization peptide mass spectrometry

1996 • 115 citations

Peptide-Mass Profiles of Polyvinylidene Difluoride-Bound Proteins by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry in the Presence of Nonionic Detergents

1996 • 113 citations

Escherichia coliproteome analysis using the gene‐protein database

1997 • 113 citations

Growth rate paradox of Salmonella typhimurium within host macrophages

1993 • 113 citations

Identification of myocardial proteins from two‐dimensional gels by peptide mass fingerprinting

1995 • 109 citations

Electrophoresis and electrofocusing in detergent containing media: A discussion of basic concepts

1981 • 108 citations

Peptide ladder sequencing by mass spectrometry using a novel, volatile degradation reagent

1994 • 107 citations

Enhancement of Bacteriophage T4 Late Transcription by Components of the T4 DNA Replication Apparatus

1989 • 107 citations

Protein mass spectrometry: applications to analytical biotechnology

1995 • 105 citations

Protein composition of the human heart: The construction of a myocardial two‐dimensional electrophoresis database

1994 • 103 citations

Extended N-terminal sequencing of proteins of archaebacterial ribosomes blotted from two-dimensional gels onto glass fiber and poly(vinylidene difluoride) membrane

1988 • 101 citations

Two‐dimensional gel protein database of Saccharomyces cerevisiae

1996 • 100 citations

Strategies for whole microbial genome sequencing and analysis

1997 • 90 citations

Amino acid analysis using derivatisation with 9-fluorenylmethyl chloroformate and reversed-phase high-performance liquid chromatography

1991 • 88 citations

Identification of transformation sensitive proteins recorded in human two‐dimensional gel protein databases by mass spectrometric peptide mapping alone and in combination with microsequencing

1994 • 87 citations

Protein identifications for aSaccharomyces cerevisiae protein database

1994 • 87 citations

Peer Reviewed: Mining Genomes with MS

1996 • 87 citations

Analysis of Global Responses by Protein and Peptide Fingerprinting of Proteins Isolated by Two‐Dimensional Gel Electrophoresis

1996 • 86 citations

Amino Acid Analysis and Protein Database Compositional Search as a Rapid and Inexpensive Method to Identify Proteins

1994 • 85 citations

Blotting efficiency investigated by using two‐dimensional electrophoresis, hydrophobic membranes and proteins from different sources

1990 • 85 citations

Direct database searching with MALDI‐PSD spectra of peptides

1995 • 84 citations

Matrix‐assisted laser‐desorption/ionization mass spectrometric approaches for the identification of gel‐separated proteins in the 5–50 pmol range

1995 • 83 citations

Construction of a human full-length cDNA bank

1994 • 82 citations

High sensitivity identification of proteins by electrospray ionization tandem mass spectrometry: Initial com‐ parison between an ion trap mass spectrometer and a triple quadrupole mass spectrometer

1997 • 82 citations

Two‐Dimensional protein map of Saccharomyces cerevisiae: Construction of a gene–protein index

1995 • 82 citations

Exploring the Mycoplasma capricolum genome: a minimal cell reveals its physiology

1995 • 80 citations

The human keratinocyte two‐dimensional gel protein database: Update 1993

1993 • 80 citations

Two‐dimensional gel electrophoresis of Escherichia coli homogenates: The Escherichia coli SWISS‐2DPAGE database

1996 • 79 citations

Applications of automated amino acid analysis using 9-fluorenylmethyl chloroformate

1991 • 79 citations

Assessment of protein sequence identity from amino acid composition data

1977 • 78 citations

Mass spectrometric and Edman sequencing of lipocortin I isolated by two-dimensional SDS/PAGE of human melanoma lysates.

1993 • 78 citations

Development of a dedicated two-dimensional gel electrophoresis system that provides optimal pattern reproducibility and polypeptide resolution.

1990 • 77 citations

Tissue and species specificity of non-histone chromatin proteins

1973 • 77 citations

Amino acid analysis on polyvinylidene difluoride membranes

1989 • 77 citations

Staining methods in gel electrophoresis, including the use of multiple detection methods

1995 • 76 citations

Rapid Identification of Yeast Proteins on Two-dimensional Gels

1996 • 75 citations

Comparing two‐dimensional electrophoretic gel images across the Internet

1997 • 75 citations

Human liver protein map: Update 1993

1993 • 75 citations

Identification of human myocardial proteins separated by two‐dimensional electrophoresis with matrix‐assisted laser desorption/ionization mass spectrometry

1996 • 75 citations

Proteome analysis of Saccharomyces cerevisiae: A methodological outline

1997 • 75 citations

Comparison of in‐gel and on‐membrane digestion methods at low to sub‐pmol level for subsequent peptide and fragment‐ion mass analysis using matrix‐assisted laser‐desorption/ionization mass spectrometry

1997 • 74 citations

Correction for Amino Acid Loss during Acid Hydrolysis of a Purified Protein

1996 • 74 citations

CD-Tagging: A New Approach to Gene and Protein Discovery and Analysis

1996 • 74 citations

Adaptation of Escherichia coli to the uncoupler of oxidative phosphorylation 2,4-dinitrophenol

1993 • 71 citations

Contacts between 16S ribosomal RNA and mRNA, within the spacer region separating the AUG initiator codon and the Shine -Dalgarno sequence; a site-directed cross-linking study

1994 • 71 citations

Quantitation of human leukocyte proteins after silver staining: A study with two‐dimensional electrophoresis

1991 • 71 citations

What is life? The next fifty years

1996 • 70 citations

Rapid Protein Identification Using N-Terminal “Sequence Tag” and Amino Acid Analysis

1996 • 70 citations

“Colored” Noise Waveforms and Quadrupole Excitation for the Dynamic Range Expansion of Fourier Transform Ion Cyclotron Resonance Mass Spectrometry

1996 • 70 citations

A shortcut to interesting human genes: peptide sequence tags, expressed-sequence tags and computers

1996 • 69 citations

Automation of micro‐preparation and enzymatic cleavage of gel electrophoretically separated proteins

1995 • 68 citations

Effect of 2-mercaptoethanol on pH gradients in isoelectric focusing

1982 • 68 citations

How reliably do amino acid composition comparisons predict sequence similarities between proteins?

1979 • 68 citations

A survey of the Mycoplasma genitalium genome by using random sequencing

1993 • 68 citations

The human myocardial two‐dimensional gel protein database: Update 1994

1994 • 67 citations

Human cellular protein patterns and their link to genome DNA sequence data: usefulness of two‐dimensional gel electrophoresis and microsequencing

1991 • 67 citations

‘Proteomic contigs’ of Mycobacterium tuberculosis and Mycobacterium bovis (BCG) using novel immobilised pH gradients

1997 • 64 citations

A qualitative and quantitative protein database approach identifies individual and groups of functionally related proteins that are differentially regulated in simian virus 40 (SV40) transformed human keratinocytes: An overview of the functional changes associated with the transformed phenotype

1994 • 63 citations

Microsequencing of proteins recorded in human two‐dimensional gel protein databases

1991 • 63 citations

Yeast Protein Database (YPD): a database for the complete proteome of Saccharomyces cerevisiae

1997 • 63 citations

Large-scale amino-acid analysis for proteome studies

1996 • 63 citations

Advantages of separations on "giant" two-dimensional gels for detection of physiologically relevant changes in the expression of protein gene-products.

1984 • 63 citations

Electrophoresis in gels containing zwitterionic groups

1981 • 62 citations

A human myocardial two‐dimensional electrophoresis database: Protein characterisation by microsequencing and immunoblotting

1992 • 62 citations

High‐performance human myocardial two‐dimensional electrophoresis database: Edition 1996

1996 • 62 citations

C‐terminal sequencing of protein

1992 • 62 citations

Cell biology and the genome projects a concerted strategy for characterizing multiprotein complexes by using mass spectrometry

1997 • 61 citations

Two‐dimensional gel electrophoresis, protein electroblotting and microsequencing: A direct link between proteins and genes

1990 • 60 citations

Improved high-performance liquid chromatography of amino acids derivatised with 9-fluorenylmethyl chloroformate

1996 • 59 citations

Functionalized membrane supports for covalent protein microsequence analysis

1991 • 59 citations

Isoelectric focusing and non‐isoelectric precipitation of ferritin in immobilized pH gradients: An improved protocol overcoming protein‐matrix interactions

1987 • 58 citations

Peptide sequence information derived by partial acid hydrolysis and matrix-assisted laser desorption/ionization mass spectrometry

1994 • 57 citations

Proteome of Salmonella typhimurium SL1344: identification of novel abundant cell envelope proteins and assignment to a two-dimensional reference map

1996 • 57 citations

Improving the detection of proteins after transfer to polyvinylidene difluoride membranes

1992 • 57 citations

Rapid identification of proteins.

1993 • 57 citations

A two‐dimensional gel protein database of noncultured total normal human epidermal keratinocytes: Identification of proteins strongly up‐regulated in psoriatic epidermis

1990 • 56 citations

Proteomic ‘contigs’ of Ochrobactrum anthropi, application of extensive pH gradients

1997 • 55 citations

Ultraviolet matrix assisted laser desorption ionization‐mass spectrometry of electroblotted proteins

1996 • 55 citations

Old phage, new insights: Two recently recognized mechanisms of transcriptional regulation in bacteriophage T4 development

1995 • 54 citations

Stability and degradation of mRNA

1991 • 54 citations

Microsequences of 145 proteins recorded in the two‐dimensional gel protein database of normal human epidermal keratinocytes

1992 • 54 citations

High-yield recovery of electroblotted proteins and cleavage fragments from a cationic polyvinylidene fluoride-based membrane

1992 • 53 citations

Osmoresponsive proteins and functional assessment strategies in Saccharomyces cerevisiae

1997 • 53 citations

Fast protein sequence determination with matrix‐assisted laser desorption and ionization mass spectrometry

1991 • 52 citations

The construction of the World Wide Web‐accessible myocardial two‐dimensional gel electrophoresis protein database “HEART‐2DPAGE”: A practical approach

1996 • 51 citations

Proteome analysis of Spiroplasma melliferum (A56) and protein characterisation across species boundaries

1997 • 50 citations

Very-high-resolution two-dimensional gel electrophoresis of proteins using giant gels

1980 • 50 citations

Identification of procaryotic developmental stages by statistical analyses of two‐dimensional gel patterns

1997 • 50 citations

Mouse liver protein database: A catalog of proteins detected by two‐dimensional gel electrophoresis

1992 • 50 citations

An Agarose‐Based Gel‐Concentration System for Microsequence and Mass Spectrometric Characterization of Proteins Previously Purified in Polyacrylamide Gels Starting at Low Picomole Levels

1995 • 50 citations

Identification of tissue proteins by amino acid analysis after purification by two-dimensional electrophoresis

1992 • 49 citations

Characterisation of Haemophilus influenzae proteins by two‐dimensional gel electrophoresis

1995 • 48 citations

Structural characterization of blotting membranes and the influence of membrane parameters for electroblotting and subsequent amino acid sequence analysis of proteins

1993 • 48 citations

Identification of proteins by their amino acid composition: An evaluation of the method

1996 • 47 citations

An attempt to resolve all the various proteins in a single human cell type by two-dimensional electrophoresis: I. Extraction of all cell proteins.

1984 • 46 citations

Towards a proteome project of cyanobacterium Synechocystis sp. strain PCC6803: Linking 130 protein spots with their respective genes

1997 • 46 citations

An attempt to resolve all the various proteins in a single human cell type by two-dimensional electrophoresis: I. Extraction of all cell proteins.

1984 • 46 citations

Molecular fish on chips

1997 • 45 citations

Development of a Haemophilus two‐dimensional protein database

1997 • 44 citations

The maize two-dimensional gel protein database: towards an integrated genome analysis program

1996 • 43 citations

A two‐dimensional electrophoresis database of human breast epithelial cell proteins

1997 • 43 citations

Secreted Antigens of Mycobacterium tuberculosis: Characterization with T Lymphocytes from Patients and Contacts after Two-Dimensional Separation

1992 • 42 citations

Protein expression during exponential growth in 0.7 M NaCl medium ofSaccharomyces cerevisiae

1996 • 42 citations

Micropreparative immobilized pH gradient two‐dimensional electrophoresis in combination with protein microsequencing for the analysis of human liver proteins

1995 • 42 citations

Identification of polypeptides on two-dimensional electrophoresis gels by amino acid composition.

1984 • 41 citations

Immobilized pH gradients in capillary tubes and two‐dimensional gel electrophoresis

1986 • 41 citations

The yeast SWISS‐2DPAGE database

1996 • 40 citations

Analysis of scaling methods to minimize experimental variations in two‐dimensional electrophoresis quantitative data: Application to the comparison of maize inbred lines

1993 • 40 citations

Direct amino acid analysis of proteins electroblotted onto polyvinylidene difluoride membrane from sodium dodecyl sulfate-polyacrylamide gel

1989 • 39 citations

Analysis of starvation conditions that allow for prolonged culturability of Vibrio vulnificus at low temperature

1996 • 38 citations

Protein analysis on a genomic scale

1995 • 37 citations

[15] Protein structure analysis by mass spectrometry

1996 • 36 citations

Characterisation of basic proteins from Spiroplasma melliferum using novel immobilised pH gradients

1997 • 36 citations

Adaptive classification of two‐dimensional gel electrophoretic spot patterns by neural networks and cluster analysis

1997 • 34 citations

Deblocking and Subsequent Microsequence Analysis of Nα-Blocked Proteins Electroblotted onto PVDF Membrane1

1992 • 34 citations

Sequencing electroblotted proteins by tandem mass spectrometry

1995 • 33 citations

Analytical and micropreparative two-dimensional electrophoresis of proteins

1991 • 33 citations

Enhanced in situ gel digestion of electrophoretically separated proteins with automated peptide elution onto mini reversed‐phase columns

1996 • 30 citations

Identification of proteins in sequence databases from amino acid composition data

1991 • 30 citations

What is life? The next fifty years

1996 • 29 citations

Hybrid isoelectric focusing: Adsorption of proteins onto immobilized pH gradient matrices and desorption by carrier ampholytes

1987 • 29 citations

The proteome of Salmonella enterica serovar typhimurium: Current progress on its determination and some applications

1997 • 28 citations

Conserved Motifs as the Basis for Recognition of Homologous Proteins Across Species Boundaries Using Peptide-mass Fingerprinting

1997 • 28 citations

Probing protein function using a combination of gene knockout and proteome analysis by mass spectrometry

1997 • 27 citations

Peptide‐mass fingerprinting and the ideal covering set for protein characterisation

1997 • 27 citations

Workshop on two‐dimensional gel protein databases

1992 • 26 citations

Identification of proteins of the yeast protein map using genetically manipulated strains and peptide-mass fingerprinting.

1996 • 26 citations

Coelectrophoresis of cardiac tissue from human, dog, rat and mouse: Towards the establishment of an integrated two‐dimensional protein database

1995 • 26 citations

Use of principal components analysis for mutation detection with two–dimensional electrophoresis protein separations

1992 • 25 citations

Cooperative relaxation of supercoils and periodic transcriptional initiation within polymerase batteries

1996 • 24 citations

Conventional isoelectric focusing and immobilized pH gradients in ‘macroporous’ polyacrylamide gels

1993 • 24 citations

Evaluation of algorithms used for cross-species proteome characterisation

1997 • 23 citations

Automated Carboxy-Terminal Sequence Analysis of Polypeptides Containing C-Terminal Proline

1995 • 22 citations

The SWISS-2DPAGE database of two-dimensional polyacrylamide gel electrophoresis.

1994 • 22 citations

Two-dimensional electrophoresis computerized processing

1988 • 22 citations

Amino acid compositions of proteins and their identities

1995 • 21 citations

The Sulfolobus solfataricus P2 genome project

1996 • 20 citations

Giant two‐dimensional gel electrophoresis: Methodological update and comparison with intermediate‐format gel systems

1990 • 20 citations

Identification of proteins of the yeast protein map using genetically manipulated strains and peptide-mass fingerprinting

1996 • 20 citations

Research on peptides and glycopeptides

1979 • 20 citations

Polypeptide cartography of Spiroplasma taiwanense

1994 • 19 citations

Amino acid analysis of proteins separated by two‐dimensional electrophoresis in maize: Isoform detection and function identification

1996 • 18 citations

Proteins and Their Amino Acid Compositions: Uniqueness, Variability, and Applications

1996 • 17 citations

Peptide fingerprints after partial acid hydrolysis: Analysis by matrix‐assisted laser desorption/ionization mass spectrometry

1994 • 16 citations

Global analysis of physiological responses in marine bacteria

1997 • 15 citations

The rat liver epithelial (RLE) cell nuclear protein database

1993 • 14 citations

Control and regulation of metabolic fluxes in microbes by substrates and enzymes

1993 • 13 citations

Basic Theories and Principles of Electrophoresis

1987 • 12 citations

The amino acid composition of 350 lymphocyte proteins

1994 • 12 citations

On the computational approach to immobilized pH gradients

1991 • 11 citations

Preparative two-dimensional polyacrylamide gel electrophoresis of rat liver ribosomal proteins and determination of their amino acid compositions

1978 • 11 citations

Convergence of amino acid compositions of certain groups of proteins aids in their identification on two‐dimensional electrophoresis gels

1997 • 9 citations

The rates of macromolecular chain elongation modulate the initiation frequencies for transcription and translation inEscherichia coli

1993 • 9 citations

Elusive control

1995 • 8 citations

Chemical protein sequencing and amino acid analysis

1993 • 8 citations

The analysis of two-dimensional polyacrylamide gels for the construction of protein databases

1992 • 8 citations

Asparaginyl endopeptidase mapping of proteins with subsequent matrix-assisted laser desorption/ionization mass spectrometry

1995 • 7 citations

A Chemical Method for the C-Terminal Sequence Analysis of Proteins

1994 • 5 citations

Amino Acid Compositions of Human Hair Fibrous Protein Components Purified with Two‐dimensional Electrophoresis

1989 • 4 citations

Identification of a conditionally essential heat shock protein in Escherichia coli

1994 • 3 citations