Characterisation of basic proteins from Spiroplasma melliferum using novel immobilised pH gradients

Improved tools for biological sequence comparison.

1988 • 11,455 citations

Whole-Genome Random Sequencing and Assembly of Haemophilus influenzae Rd

1995 • 5,571 citations

Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes.

1987 • 5,066 citations

Life with 6000 Genes

1996 • 4,125 citations

High resolution two-dimensional electrophoresis of basic as well as acidic proteins

1977 • 3,556 citations

The Minimal Gene Complement of Mycoplasma genitalium

1995 • 2,499 citations

Sequence Analysis of the Genome of the Unicellular Cyanobacterium Synechocystis sp. Strain PCC6803. II. Sequence Determination of the Entire Genome and Assignment of Potential Protein-coding Regions (Supplement)

1996 • 2,455 citations

Two‐dimensional electrophoresis. The current state of two‐dimensional electrophoresis with immobilized pH gradients

1988 • 2,431 citations

Sequence Analysis of the Genome of the Unicellular Cyanobacterium Synechocystis sp. Strain PCC6803. II. Sequence Determination of the Entire Genome and Assignment of Potential Protein-coding Regions

1996 • 2,046 citations

Complete Genome Sequence of the Methanogenic Archaeon, Methanococcus jannaschii

1996 • 2,043 citations

Femtomole sequencing of proteins from polyacrylamide gels by nano-electrospray mass spectrometry

1996 • 1,687 citations

Rapid identification of proteins by peptide-mass fingerprinting

1993 • 1,619 citations

Linking genome and proteome by mass spectrometry: Large-scale identification of yeast proteins from two dimensional gels

1996 • 1,430 citations

Identifying proteins from two-dimensional gels by molecular mass searching of peptide fragments in protein sequence databases.

1993 • 1,301 citations

Complete Sequence Analysis of the Genome of the Bacterium Mycoplasma Pneumoniae

1996 • 1,143 citations

Use of mass spectrometric molecular weight information to identify proteins in sequence databases

1993 • 936 citations

Progress with gene‐product mapping of the Mollicutes: Mycoplasma genitalium

1995 • 934 citations

From Proteins to Proteomes: Large Scale Protein Identification by Two-Dimensional Electrophoresis and Arnino Acid Analysis

1996 • 837 citations

Two‐dimensional electrophoresis of proteins: An updated protocol and implications for a functional analysis of the genome

1995 • 724 citations

Improved Resolution and Very High Sensitivity in MALDI TOF of Matrix Surfaces Made by Fast Evaporation

1994 • 682 citations

Peptide Mass Maps: A Highly Informative Approach to Protein Identification

1993 • 678 citations

Protein Identification by Mass Profile Fingerprinting

1993 • 595 citations

Reference points for comparisons of two‐dimensional maps of proteins from different human cell types defined in a pH scale where isoelectric points correlate with polypeptide compositions

1994 • 523 citations

A nonlinear wide‐range immobilized pH gradient for two‐dimensional electrophoresis and its definition in a relevant pH scale

1993 • 386 citations

Mass spectrometric approaches for the identification of gel‐separated proteins

1995 • 378 citations

Immobilized pH Gradients: Theory and Methodology

1990 • 363 citations

Protein-blotting on Polybrene-coated glass-fiber sheets. A basis for acid hydrolysis and gas-phase sequencing of picomole quantities of protein previously separated on sodium dodecyl sulfate/polyacrylamide gel

1985 • 288 citations

Matrix-assisted laser desorption ionization mass spectrometry of proteins electroblotted after polyacrylamide gel electrophoresis

1994 • 223 citations

Electrospray mass spectrometry for protein characterization

1995 • 218 citations

Rapid mass spectrometric peptide sequencing and mass matching for characterization of human melanoma proteins isolated by two-dimensional PAGE.

1995 • 206 citations

Micropreparative two‐dimensional electrophoresis allowing the separation of samples containing milligram amounts of proteins

1993 • 191 citations

Two-dimensional electrophoresis

1991 • 185 citations

Protein identification by solid phase microextraction—capillary zone electrophoresis—microelectrospray—tandem mass spectrometry

1996 • 184 citations

Site-Specific Characterization of Glycoprotein Carbohydrates by Exoglycosidase Digestion and Laser-Desorption Mass Spectrometry

1994 • 162 citations

Identification of proteins in polyacrylamide gels by mass spectrometric peptide mapping combined with database search

1994 • 161 citations

Interlaboratory reproducibility of yeast protein patterns analyzed by immobilized pH gradient two‐dimensional gel electrophoresis

1995 • 152 citations

Improved mass accuracy in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry of peptides

1994 • 144 citations

Cross‐species identification of proteins separated by two‐dimensional gel electrophoresis using matrix‐assisted laser desorption ionisation/time‐of‐flight mass spectrometry and amino acid composition

1995 • 127 citations

Peptide-Mass Profiles of Polyvinylidene Difluoride-Bound Proteins by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry in the Presence of Nonionic Detergents

1996 • 113 citations

Identification of myocardial proteins from two‐dimensional gels by peptide mass fingerprinting

1995 • 109 citations

New Rhizobium leguminosarum Flavonoid-Induced Proteins Revealed by Proteome Analysis of Differentially Displayed Proteins

1997 • 91 citations

Analysis of Global Responses by Protein and Peptide Fingerprinting of Proteins Isolated by Two‐Dimensional Gel Electrophoresis

1996 • 86 citations

Amino Acid Analysis and Protein Database Compositional Search as a Rapid and Inexpensive Method to Identify Proteins

1994 • 85 citations

Matrix‐assisted laser‐desorption/ionization mass spectrometric approaches for the identification of gel‐separated proteins in the 5–50 pmol range

1995 • 83 citations

Rapid Identification of Yeast Proteins on Two-dimensional Gels

1996 • 75 citations

Heterogeneity of Genome Sizes within the Genus Spiroplasma

1995 • 73 citations

Advantages of separations on "giant" two-dimensional gels for detection of physiologically relevant changes in the expression of protein gene-products.

1984 • 63 citations

Isoelectric focusing and non‐isoelectric precipitation of ferritin in immobilized pH gradients: An improved protocol overcoming protein‐matrix interactions

1987 • 58 citations

Proteomic ‘contigs’ of Ochrobactrum anthropi, application of extensive pH gradients

1997 • 55 citations

Proteome analysis of Spiroplasma melliferum (A56) and protein characterisation across species boundaries

1997 • 50 citations

Very-high-resolution two-dimensional gel electrophoresis of proteins using giant gels

1980 • 50 citations

Identification of tissue proteins by amino acid analysis after purification by two-dimensional electrophoresis

1992 • 49 citations

Identification of proteins by their amino acid composition: An evaluation of the method

1996 • 47 citations

An attempt to resolve all the various proteins in a single human cell type by two-dimensional electrophoresis: I. Extraction of all cell proteins.

1984 • 46 citations

An attempt to resolve all the various proteins in a single human cell type by two-dimensional electrophoresis: I. Extraction of all cell proteins.

1984 • 46 citations

Immobilized pH gradients in capillary tubes and two‐dimensional gel electrophoresis

1986 • 41 citations

IPGMAKER: A program for IBM‐compatible personal computers to create and test recipes for immobilized pH gradients

1990 • 37 citations

Hybrid isoelectric focusing: Adsorption of proteins onto immobilized pH gradient matrices and desorption by carrier ampholytes

1987 • 29 citations

Giant two‐dimensional gel electrophoresis: Methodological update and comparison with intermediate‐format gel systems

1990 • 20 citations

Multiplication and persistence of Spiroplasma melliferum strain A56 in experimentally infected suckling mice

1991 • 19 citations

Proteins and Their Amino Acid Compositions: Uniqueness, Variability, and Applications

1996 • 17 citations

The amino acid composition of 350 lymphocyte proteins

1994 • 12 citations