The focusing positions of polypeptides in immobilized pH gradients can be predicted from their amino acid sequences

Two‐dimensional electrophoresis. The current state of two‐dimensional electrophoresis with immobilized pH gradients

1988 • 2,431 citations

Isoelectric focusing in immobilized pH gradients: Principle, methodology and some applications

1982 • 963 citations

pK a Prediction for Organic Acids and Bases

1981 • 642 citations

The SWISS-PROT protein sequence data bank

1991 • 341 citations

Covalent structure of human haptoglobin: a serine protease homolog.

1980 • 244 citations

Cloning and expression of a cDNA for human thioredoxin.

1988 • 239 citations

Nucleotide sequence of the haptoglobin and haptoglobin-related gene pair. The haptoglobin-related gene contains a retrovirus-like element.

1985 • 161 citations

Structure and expression of the human haptoglobin locus.

1985 • 147 citations

A two‐dimensional gel database of human plasma proteins

1991 • 143 citations

High-resolution three-dimensional structure of reduced recombinant human thioredoxin in solution

1991 • 141 citations

Plasma protein map: An update by microsequencing

1992 • 135 citations

Microheterogeneity of human serum transferrin: A biological phenomenon studied by isoelectric focusing in immobilized pH gradients

1988 • 132 citations

Human liver protein map: A reference database established by microsequencing and gel comparison

1992 • 129 citations

Identification and characterization of human haptoglobin cDNA.

1983 • 101 citations

Temperature‐dependent spot positional variability in two‐dimensional polypeptide patterns

1991 • 81 citations

Isoelectric focusing in immobilized pH gradients in presence of urea and neutral detergents

1983 • 61 citations

Isoelectric points of proteins, determined by isoelectric focusing in the presence of urea and ethanol

1979 • 53 citations

IPGMAKER: A program for IBM‐compatible personal computers to create and test recipes for immobilized pH gradients

1990 • 37 citations

Pouring reproducible gradients in gels under computer control: new devices for simultaneous delivery of two independent gradients, for more flexible slope and pH range of immobilized pH gradients.

1984 • 36 citations

Isoelectric focusing as a method for the characterization of ampholytes

1978 • 36 citations

Isoelectric focusing of apolipoproteins in immobilized pH gradients: Improved determination of apolipoprotein Ephenotypes

1988 • 24 citations

Evolution of haptoglobin: comparison of complementary DNA encodingHpα^1SandHpα^2FS

1984 • 17 citations

Two‐dimensional electrophoresis analysis of human serum proteins during the acute‐phase response

1992 • 13 citations