Immunohistochemical markers of cellular proliferation: achievements, problems and prospects

Molecular Biology of the Cell

2007 • 4,686 citations

Cell cycle analysis of a cell proliferation-associated human nuclear antigen defined by the monoclonal antibody Ki-67.

1984 • 4,180 citations

Checkpoints: Controls That Ensure the Order of Cell Cycle Events

1989 • 3,036 citations

Universal control mechanism regulating onset of M-phase

1990 • 2,882 citations

Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation

1983 • 2,745 citations

Monoclonal Antibody to 5-Bromo- and 5-Iododeoxyuridine: A New Reagent for Detection of DNA Replication

1982 • 2,648 citations

G ₁ Events and Regulation of Cell Proliferation

1989 • 2,203 citations

Cyclin/PCNA is the auxiliary protein of DNA polymerase-δ

1987 • 1,766 citations

Proliferating cell nuclear antigen (PCNA) immunolocalization in paraffin sections: An index of cell proliferation with evidence of deregulated expression in some, neoplasms

1990 • 1,454 citations

Functional identity of proliferating cell nuclear antigen and a DNA polymerase-δ auxiliary protein

1987 • 1,074 citations

The retinoblastoma protein is phosphorylated during specific phases of the cell cycle

1989 • 1,031 citations

Phosphorylation of the retinoblastoma gene product is modulated during the cell cycle and cellular differentiation

1989 • 990 citations

Flow cytometric measurement of total DNA content and incorporated bromodeoxyuridine.

1983 • 978 citations

Genes specifically expressed at growth arrest of mammalian cells

1988 • 954 citations

Existence of two populations of cyclin/proliferating cell nuclear antigen during the cell cycle: association with DNA replication sites.

1987 • 945 citations

The product of the retinoblastoma susceptibility gene has properties of a cell cycle regulatory element

1989 • 931 citations

Autoantibody to a Nuclear Antigen in Proliferating Cells

1978 • 881 citations

The relation between cell proliferation and the vascular system in a transplanted mouse mammary tumour

1968 • 764 citations

Topoisomerase II is a structural component of mitotic chromosome scaffolds.

1985 • 751 citations

Dominoes and Clocks: the Union of Two Views of the Cell Cycle

1989 • 750 citations

Bromodeoxyuridine immunohistochemical determination of the lengths of the cell cycle and the DNA-synthetic phase for an anatomically defined population

1989 • 746 citations

Monoclonal antibody analysis of the proliferating cell nuclear antigen (PCNA) structural conservation and the detection of a nucleolar form

1990 • 736 citations

Growth Kinetics of Tumours

1978 • 691 citations

Mutations of phosphorylation sites in lamin A that prevent nuclear lamina disassembly in mitosis

1990 • 642 citations

Identification of a set of genes expressed during the G0/G1 transition of cultured mouse cells.

1985 • 589 citations

Analysis of proliferative grade using anti-PCNA/cyclin monoclonal antibodies in fixed, embedded tissues. Comparison with flow cytometric analysis.

1989 • 582 citations

Stem cells: the generation and maintenance of cellular diversity

1989 • 504 citations

An auxiliary protein for DNA polymerase-delta from fetal calf thymus.

1986 • 500 citations

A search for differential polypeptide synthesis throughout the cell cycle of HeLa cells.

1980 • 465 citations

Review: assessment of cell proliferation in histological material.

1990 • 456 citations

Regulation of Proliferating Cell Nuclear Antigen during the Cell Cycle

1989 • 447 citations

Inhibition of Cellular Proliferation by Antisense Oligodeoxynucleotides to PCNA Cyclin

1988 • 425 citations

Identification of cell cycle-regulated phosphorylation sites on nuclear lamin C

1990 • 425 citations

The retinoblastoma susceptibility gene product undergoes cell cycle-dependent dephosphorylation and binding to and release from SV40 large T

1990 • 402 citations

Apoptosis: Cell death in tissue regulation

1987 • 393 citations

S Phase of the Cell Cycle

1989 • 366 citations

The clinical assessment of proliferation and growth in human tumours: Evaluation of methods and applications as prognostic variables

1990 • 341 citations

A nuclear antigen associated with cell proliferation and blast transformation.

1981 • 333 citations

The prognostic value of Ki67 immunostaining in non‐Hodgkin's lymphoma

1988 • 314 citations

Measurement of cell kinetics in human tumours in vivo using bromodeoxyuridine incorporation and flow cytometry

1988 • 310 citations

Changes in the nuclear distribution of cyclin (PCNA) but not its synthesis depend on DNA replication.

1985 • 284 citations

Cell loss as a factor in the growth rate of human tumours

1967 • 281 citations

Monoclonal antibodies to a nuclear protein (PCNA/cyclin) associated with DNA replication

1987 • 268 citations

Ki67 immunostaining in primary breast cancer: pathological and clinical associations

1989 • 267 citations

Ki-67 detects a nuclear matrix-associated proliferation-related antigen: II. Localization in mitotic cells and association with chromosomes

1989 • 263 citations

Preparation and preliminary characterization of monoclonal antibodies against human DNA polymerase alpha.

1982 • 257 citations

Correlation between DNA flow cytometric and nucleolar organizer region data in non‐Hodgkin's lymphomas

1988 • 256 citations

Proliferative index in breast carcinoma determined in situ by Ki67 immunostaining and its relationship to clinical and pathological variables

1987 • 253 citations

A comparison of nucleolar organizer region staining and Ki‐67 immunostaining in non‐Hodgkin's lymphoma

1988 • 248 citations

Changes in cyclin/proliferating cell nuclear antigen distribution during DNA repair synthesis.

1988 • 248 citations

The Biology of Epithelial Cell Populations

1985 • 246 citations

DNA repair synthesis in human fibroblasts requires DNA polymerase delta.

1988 • 245 citations

Proteins C23 and B23 are the major nucleolar silver staining proteins

1979 • 239 citations

Cyclin: A nuclear protein whose level correlates directly with the proliferative state of normal as well as transformed cells

1984 • 231 citations

The cell cycle associated change of the Ki‐67 reactive nuclear antigen expression

1987 • 228 citations

Independent prognostic significance of a nuclear proliferation antigen in diffuse large cell lymphomas as determined by the monoclonal antibody Ki-67

1988 • 227 citations

A role for neuropeptides in the control of cell proliferation

1987 • 225 citations

The long‐term prognostic significance of the thymidine labelling index in breast cancer

1984 • 225 citations

Mediated transport of nucleosides in human erythrocytes. Specific binding of the inhibitor nitrobenzylthioinosine to nucleoside transport sites in the erythrocyte membrane

1974 • 224 citations

Ki-67 detects a nuclear matrix-associated proliferation-related antigen : I. Intracellular localization during interphase

1989 • 223 citations

Cell‐Cycle Analysis Using A Monoclonal Antibody to Brdurd

1984 • 205 citations

Existence of High Abundance Antiproliferative mRNA's in Senescent Human Diploid Fibroblasts

1986 • 194 citations

Characterization of proliferating cell nuclear antigen recognized by autoantibodies in lupus sera.

1984 • 189 citations

CORRELATION OF TRANSFERRIN RECEPTOR EXPRESSION WITH HISTOLOGICAL CLASS AND OUTCOME IN NON-HODGKIN LYMPHOMA

1983 • 189 citations

Nuclear distribution of the Ki-67 antigen during the cell cycle: comparison with growth fraction in human breast cancer cells.

1989 • 183 citations

Cell-cycle regulation of histone gene expression

1986 • 183 citations

Relationship between labeling indices of KI-67 and brdurd in human malignant tumors

1988 • 177 citations

The messenger RNA sequences in growing and resting mouse fibroblasts

1975 • 175 citations

Disruption of the keratin filament network during epithelial cell division.

1982 • 172 citations

Increased nuclear cyclin/PCNA antigen staining of non S‐phase transformed human amnion cells engaged in nucleotide excision DNA repair

1986 • 172 citations

Intracellular localization of human DNA polymerase alpha with monoclonal antibodies.

1982 • 161 citations

Mitosis

1989 • 155 citations

Argyrophilic nuclear and nucleolar proteins of Xenopus laevis oocytes identified by gel electrophoresis

1982 • 153 citations

Identification of numatrin, the nuclear matrix protein associated with induction of mitogenesis, as the nucleolar protein B23. Implication for the role of the nucleolus in early transduction of mitogenic signals.

1988 • 151 citations

Determination of the growth fraction in cell suspensions by flow cytometry using the monoclonal antibody Ki-67

1986 • 150 citations

Transcriptional and posttranscriptional regulation of the proliferating cell nuclear antigen gene.

1990 • 143 citations

Characterization of a large form of DNA polymerase δ from HeLa cells that is insensitive to proliferating cell nuclear antigen

1989 • 141 citations

Gene for proliferating-cell nuclear antigen (DNA polymerase delta auxiliary protein) is present in both mammalian and higher plant genomes.

1989 • 140 citations

Thymidine labeling index and Ki-67 growth fraction in lesions of the breast.

1989 • 138 citations

Commentary dna polymerase δ/PCNA: Actions and interactions

1990 • 136 citations

Gene expression of human DNA polymerase alpha during cell proliferation and the cell cycle.

1988 • 136 citations

Ki‐67 derived proliferative activity in colorectal adenocarcinoma with prognostic correlations

1988 • 135 citations

Perspectives on clonogenic tumor cells, stem cells, and oncogenes.

1984 • 133 citations

Further evidence that phosphoprotein C23 (110 kD/pI 5.1) is the nucleolar silver staining protein

1984 • 133 citations

A 57,000-mol-wt protein uniquely present in nonproliferating cells and senescent human fibroblasts.

1985 • 125 citations

Proliferative activity of cells in the synovium as demonstrated by a monoclonal antibody, Ki67

1987 • 114 citations

Comparison of monoclonal antibody Ki-67 reactivity with grade and DNA flow cytometry of breast carcinomas

1988 • 113 citations

New approach to assessing lung tumours in man.

1986 • 113 citations

Relationship between interphasic nucleolar organizer regions and growth rate in two neuroblastoma cell lines.

1989 • 110 citations

Involvement of proliferating cell nuclear antigen (cyclin) in DNA replication in living cells.

1989 • 107 citations

Simultaneous staining of exponentially growing versus plateau phase cells with the proliferation‐associated antibody Ki‐67 and propidium iodide: analysis by flow cytometry

1987 • 100 citations

A Stem Cell Model of Human Tumor Growth: Implications for Tumor Cell Clonogenic Assays

1983 • 95 citations

Autoantibody to the proliferating cell nuclear antigen neutralizes the activity of the auxiliary protein for DNA polymerase delta

1987 • 92 citations

Suppression of tumorigenicity in hybrids of normal and oncogene-transformed CHEF cells.

1985 • 91 citations

Importance of introns in the growth regulation of mRNA levels of the proliferating cell nuclear antigen gene.

1990 • 87 citations

Implications of kinetic heterogeneity in clinical oncology.

1985 • 84 citations

Purification and N-terminal amino acid sequence of proliferating cell nuclear antigen (PCNA)/cyclin and development of ELISA for anti-PCNA antibodies.

1985 • 82 citations

The nucleolar cycle in man

1977 • 81 citations

Immunohistologic demonstration of abnormal colonic crypt cell kinetics in ulcerative colitis

1985 • 81 citations

Rapid disappearance of statin, a nonproliferating and senescent cell-specific protein, upon reentering the process of cell cycling.

1985 • 79 citations

A comparison of three methods for the determination of the growth fraction in non-Hodgkin's lymphoma

1987 • 78 citations

Comparative Analysis of Different Approaches to Investigate Cell Kinetics

1988 • 77 citations

Cell proliferation in non-Hodgkin's lymphomas. Digital image analysis of Ki-67 antibody staining.

1989 • 75 citations

Do AgNOR counts reflect cellular ploidy or cellular proliferation? A study of trophoblastic tissue

1990 • 69 citations

Application of a unique monoclonal antibody as a marker for nonproliferating subpopulations of cells of some tissue.

1985 • 68 citations

Proliferating cell nuclear antigen (PCNA) and human malignant tumor nucleolar antigens (HMTNA) in nucleoli of human hematological malignancies

1983 • 67 citations

Proliferating cell nuclear antigen (PCNA/cyclin) immunocytochemistry as a labeling index in mouse lung tissues

1989 • 65 citations

The relationship between number of interphase NORs and nor‐bearing chromosomes in non‐Hodgkin's lymphoma

1989 • 64 citations

Immunohistochemical analysis of sarcoid granulomas. Evaluation of Ki67+ and interleukin-1+ cells.

1988 • 63 citations

Individual nuclei in polykaryons can control cyclin distribution and DNA synthesis.

1985 • 63 citations

Cell cycle-dependent reactivity with the monoclonal antibody Ki-67 during myeloid cell differentiation

1988 • 62 citations

A rapid method to determine proliferation patterns of normal and malignant tissues by H3 mRNA in situ hybridization.

1990 • 61 citations

Relationship between the Ag-NOR proteins and ribosomal chromatin in Situ during drug-induced RNA synthesis inhibition

1984 • 60 citations

Disapparence of statin, a protein marker for non-proliferating and senescent cells, following serum-stimulated cell cycle entry

1986 • 59 citations

Nucleolar silver-staining patterns related to cell cycle phase and cell generation of PHA-stimulated human lymphocytes.

1984 • 58 citations

Evolutionary conservation in various mammalian species of the human proliferation-associated epitope recognized by the Ki-67 monoclonal antibody.

1989 • 56 citations

Phenotyping of proliferating lymphocytes in angioimmunoblastic lymphadenopathy and related lesions by the double immunoenzymatic staining technique.

1987 • 55 citations

Identification and partial characterization of a Mr 40,000 nucleolar antigen associated with cell proliferation.

1987 • 53 citations

The number of silver-staining NORs (rDNA) in lymphocytes of newborns and its relationship to human developemnt

1986 • 51 citations

Immunohistochemical determination of growth fractions in human permanent cell lines and lymphoid tumours: a critical comparison of the monoclonal antibodies OKT9 and Ki‐67

1987 • 50 citations

Identification of a nuclear polypeptide ("cyclin") whose relative proportion is sensitive to changes in the rate of cell proliferation and to transformation.

1982 • 47 citations

Immunocytochemical evaluation of the percentage of proliferating cells in pathological bone marrow and peripheral blood samples with the Ki‐67 and anti‐bromo‐deoxyuridine monoclonal antibodies

1988 • 47 citations

Cell kinetics as a prognostic marker in locally advanced breast cancer.

1987 • 45 citations

Invited review Oncogenes, growth, and the cell cycle: an overview

1989 • 44 citations

Quantitation of estrogen receptor content and Ki-67 staining in breast carcinoma by the microTICAS image analysis system.

1987 • 41 citations

Quantitation and Estimation of Lymphocyte Subsets in Tissue Section: Comparison with Flow Cytometry

1987 • 40 citations

Immunochemical and biochemical analysis of the proliferating cell nuclear antigen (PCNA) in HeLa cells

1986 • 38 citations

Nucleoside transport and proliferative rate in human thymocytes and lymphocytes

1989 • 37 citations

Monoclonal antibodies against bromodeoxyuridine

1985 • 36 citations

Immunodetection in fine-needle aspirates and multiparametric (SAMBA) image analysis. Receptors (monoclonal antiestrogen and antiprogesterone) and growth fraction (monoclonal Ki67) evaluation in breast carcinomas

1989 • 34 citations

Nucleoside transport in acute leukaemia and lymphoma: close relation to proliferative rate

1989 • 33 citations

Contact‐inhibition‐induced quiescent state is marked by intense nuclear expression of Statin

1987 • 33 citations

Double Immunoenzymatic Detection of Surface Phenotype of Proliferating Lymphocytes In Situ with Monoclonal Antibodies Against DNA Polymerase α and Lymphocyte Membrane Antigens

1987 • 32 citations

Chromosomal Nonhistone Proteins

2018 • 31 citations

Monoclonal antibody against a nuclear matrix antigen in proliferating human cells

1987 • 26 citations

Effect of differentiation on the expression of a nucleolar antigen with a molecular weight of 145,000 in HL-60 cells.

1987 • 25 citations

Cell cycle dependent activities of DNA polymerases .alpha. and .delta. in Chinese hamster ovary cells

1987 • 25 citations

Cell kinetics as a prognostic tool in patients with metastatic malignant melanoma of the skin

1987 • 24 citations

Changes in a nuclear matrix antigen during the cell cycle: interphase and mitotic cells

1988 • 23 citations

Proliferation in non-Hodgkin's lymphoma: a comparison of Ki-67 staining on fine needle aspiration and cryostat sections.

1990 • 23 citations

In situ Demonstration of Renal Tubular Regeneration Using the Monoclonal Antibody Ki67

1988 • 22 citations

Immunocytochemical staining of proliferating cells in fine needle aspiration smears of primary and metastatic breast tumours

1988 • 21 citations

Measurement of the S‐phase duration in normal and abnormal human endometrium by in vitro double labelling with bromodeoxyuridine and tritiated thymidine

1989 • 21 citations

Immunocytochemical characterization of a monoclonal antibody that recognizes mitosing cells.

1985 • 21 citations

The differentiation process of intestinal epithelial cells is associated with the appearance of statin, a non-proliferation-specific nuclear protein

1990 • 21 citations

Detection of the growth fraction in colorectal tumours by a monoclonal antibody against DNA polymerase α

1990 • 17 citations

Characterization of two populations of statin and the relationship of their syntheses to the state of cell proliferation.

1990 • 16 citations

Immunochemical studies of DNA polymerase δ: Relationships with DNA polymerase α

1989 • 15 citations

MONOCLONAL ANTIBODY Ki‐67 AS A PROLIFERATION MARKER

1987 • 13 citations

A flow cytometric assay for the determination of cell proliferation with a monoclonal antibody directed against DNA‐methyltransferase

1989 • 11 citations

Immunocytochemical analysis of cytocentrifuged fine needle aspirates. A study based on lung tumors aspirated in vitro.

1989 • 11 citations

Regional variations in proliferation rate and degree of differentiation in B16 melanomas

1984 • 11 citations

Proliferative Cell Index in Endometrial Adenocarcinoma of Different Nuclear Grades

1989 • 11 citations

An immunocytochemical study of the proliferating cell nuclear matrix antigen p125/6.5 during rat spermatogenesis

1989 • 10 citations

The use of a monoclonal antibody against a proliferating cell nuclear matrix antigen in the study of solid human tumors

1989 • 10 citations

Isolation and characterization of complementary DNA to proliferating cell nucleolar antigen P40.

1989 • 9 citations

Characterization of a nuclear antigen (Mr 150,000) associated with cell proliferation.

1988 • 8 citations

Changes in cytoskeleton-associated molecules in cells with different degrees of proliferation and metastatic ability

1989 • 1 citations

Deleted Work

1955 • 0 citations