The Amino-terminal Charge and Core Region Hydrophobicity Interdependently Contribute to the Function of Signal Sequences

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

Experiments in molecular genetics

1972 • 28,260 citations

Culture Medium for Enterobacteria

1974 • 3,002 citations

Signal sequences

1989 • 417 citations

Azide-resistant mutants of Escherichia coli alter the SecA protein, an azide-sensitive component of the protein export machinery.

1990 • 311 citations

Phosphatidylglycerol is involved in protein translocation across Escherichia coli inner membranes

1988 • 263 citations

Modulation of Folding Pathways of Exported Proteins by the Leader Sequence

1988 • 243 citations

Signal peptides: exquisitely designed transport promoters

1994 • 231 citations

SecA interacts with secretory proteins by recognizing the positive charge at the amino terminus of the signal peptide in Escherichia coli.

1990 • 229 citations

Net N-C charge imbalance may be important for signal sequence function in bacteria

1986 • 176 citations

Predicted secondary structures of amino‐terminal extension sequences of secreted proteins

1979 • 172 citations

Membrane protein topology: effects of delta mu H+ on the translocation of charged residues explain the ‘positive inside’ rule.

1994 • 148 citations

Hsc70-binding Peptides Selected from a Phage Display Peptide Library that Resemble Organellar Targeting Sequences

1995 • 105 citations

The translocation of negatively charged residues across the membrane is driven by the electrochemical potential: evidence for an electrophoresis-like membrane transfer mechanism.

1995 • 104 citations

Idealization of the hydrophobic segment of the alkaline phosphatase signal peptide

1986 • 98 citations

Conserved residues of the leader peptide are essential for cleavage by leader peptidase.

1985 • 90 citations

Analysis of mutational alterations in the hydrophilic segment of the maltose-binding protein signal peptide

1989 • 79 citations

OmpF-Lpp signal sequence mutants with varying charge hydrophobicity ratios provide evidence for a phosphatidylglycerol-signal sequence interaction during protein translocation across the Escherichia coli inner membrane

1993 • 61 citations

Functional limits of conformation, hydrophobicity, and steric constraints in prokaryotic signal peptide cleavage regions. Wild type transport by a simple polymeric signal sequence.

1991 • 61 citations

Titration of protein transport activity by incremental changes in signal peptide hydrophobicity

1993 • 58 citations

The requirement of a positive charge at the amino terminus can be compensated for by a longer central hydrophobic stretch in the functioning of signal peptides.

1992 • 54 citations

Physical and Conformational Properties of Synthetic Idealized Signal Sequences Parallel Their Biological Function

1995 • 45 citations

Mode of Insertion of the Signal Sequence of a Bacterial Precursor Protein into Phospholipid Bilayers As Revealed by Cysteine-Based Site-Directed Spectroscopy

1996 • 42 citations

Energy requirements for protein translocation across the Escherichia coli inner membrane

1991 • 30 citations

Signal peptide hydrophobicity is finely tailored for function

1994 • 30 citations

Effect of alteration of charged residues at the N termini of signal peptides on protein export in Bacillus subtilis

1994 • 30 citations

SecA-dependence of the translocation of a large periplasmic loop in theEscherichia coliMalF inner membrane protein is a function of sequence context

1995 • 28 citations

Precursor-specific requirements for SecA, SecB, and delta muH+ during protein export of Escherichia coli.

1994 • 25 citations