Polypyrimidine tract sequences direct selection of alternative branch sites and influence protein binding

Oligoribonucleotide synthesis using T7 RNA polymerase and synthetic DNA templates

1987 • 2,098 citations

Genetic applications of an inverse polymerase chain reaction.

1988 • 1,867 citations

Splicing of Messenger RNA Precursors

1987 • 919 citations

Biochemical Mechanisms of Constitutive and Regulated Pre-mRNA Splicing

1991 • 708 citations

ALTERNATIVE SPLICING IN THE CONTROL OF GENE EXPRESSION

1989 • 701 citations

PRE-mRNA SPLICING

1986 • 673 citations

U2 as well as U1 small nuclear ribonucleoproteins are involved in premessenger RNA splicing

1985 • 568 citations

Cloning and domain structure of the mammalian splicing factor U2AF

1992 • 566 citations

A factor, U2AF, is required for U2 snRNP binding and splicing complex assembly

1988 • 535 citations

Recognition of the TACTAAC box during mRNA splicing in yeast involves base pairing to the U2-like snRNA

1987 • 508 citations

Heterogeneous Nuclear Ribonucleoproteins: Role in RNA Splicing

1986 • 399 citations

Identification, purification, and biochemical characterization of U2 small nuclear ribonucleoprotein auxiliary factor.

1989 • 375 citations

Cloning and characterization of PSF, a novel pre-mRNA splicing factor.

1993 • 359 citations

Identification and purification of a 62,000-dalton protein that binds specifically to the polypyrimidine tract of introns.

1989 • 349 citations

Characterization and molecular cloning of polypyrimidine tract-binding protein: a component of a complex necessary for pre-mRNA splicing.

1991 • 338 citations

Mammalian pre-mRNA branch site selection by U2 snRNP involves base pairing.

1989 • 314 citations

The organization of 3' splice-site sequences in mammalian introns.

1989 • 310 citations

The protein Sex-lethal antagonizes the splicing factor U2AF to regulate alternative splicing of transformer pre-mRNA

1993 • 309 citations

Mechanisms of Alternative Pre-mRNA Splicing

1991 • 257 citations

Mutually exclusive splicing of α-tropomyosin exons enforced by an unusual lariat branch point location: Implications for constitutive splicing

1989 • 238 citations

U1 snRNP targets an essential splicing factor, U2AF65, to the 3' splice site by a network of interactions spanning the exon.

1992 • 225 citations

Alternative mRNA Splicing

1992 • 219 citations

The role of the mammalian branchpoint sequence in pre-mRNA splicing.

1988 • 215 citations

The 35-kDa mammalian splicing factor SC35 mediates specific interactions between U1 and U2 small nuclear ribonucleoprotein particles at the 3' splice site.

1992 • 198 citations

UACUAAC is the preferred branch site for mammalian mRNA splicing.

1989 • 180 citations

Polypyrimidine tract binding protein interacts with sequences involved in alternative splicing of beta-tropomyosin pre-mRNA.

1992 • 157 citations

A mutational analysis of the polypyrimidine tract of introns. Effects of sequence differences in pyrimidine tracts on splicing

1993 • 146 citations

Targeted snRNP depletion reveals an additional role for mammalian U1 snRNP in spliceosome assembly

1990 • 143 citations

The spliceosome assembly pathway in mammalian extracts.

1992 • 111 citations

Three protein factors (SF1, SF3 and U2AF) function in pre-splicing complex formation in addition to snRNPs.

1991 • 104 citations

Alternative splicing of beta-tropomyosin pre-mRNA: cis-acting elements and cellular factors that block the use of a skeletal muscle exon in nonmuscle cells.

1991 • 98 citations

In vitro splicing of mutually exclusive exons from the chicken beta-tropomyosin gene: role of the branch point location and very long pyrimidine stretch.

1990 • 97 citations

Splicing of SV40 early pre-mRNA to large T and small t mRNAs utilizes different patterns of lariat branch sites

1987 • 93 citations

Branch point selection in alternative splicing of tropomyosin pre-mRNAs

1989 • 89 citations

The 216-nucleotide intron of the E1A pre-mRNA contains a hairpin structure that permits utilization of unusually distant branch acceptors.

1989 • 73 citations

Unusual branch point selection in processing of human growth hormone pre-mRNA.

1988 • 72 citations

Elements regulating an alternatively spliced exon of the rat fibronectin gene.

1993 • 62 citations

The Spliceosome Assembly Pathway in Mammalian Extracts

1992 • 51 citations

In vitro splicing of adenovirus E1A transcripts: characterization of novel reactions and of multiple branch points abnormally far from the 3′ splice site

1988 • 50 citations

Polypyrimidine tracts and their binding proteins: Regulatory sites for posttranscriptional modulation of gene expression

1993 • 50 citations

Alternative pre-mrna splicing: Factors involved in splice site selection

1994 • 50 citations

Alternative Splicing of a Human α-Tropomyosin Muscle-Specific Exon: Identification of Determining Sequences

1992 • 47 citations

Unusual Branch Point Selection in Processing of Human Growth Hormone Pre-mRNA

1988 • 35 citations

In vitrosplicing of fibronectin pre-mRNAs

1990 • 34 citations

The 216-Nucleotide Intron of the E1A Pre-mRNA Contains a Hairpin Structure That Permits Utilization of Unusually Distant Branch Acceptors

1989 • 30 citations

Alterations in the Polypyrimidine Sequence Affect the in vitro Splicing Reactions Catalyzed by HeLa cell-Free Preparations

1989 • 28 citations

Elements Regulating an Alternatively Spliced Exon of the Rat Fibronectin Gene

1993 • 23 citations

Characterization of HeLa Nuclear Factors Which Interact with a Conditionally Processed Rat Fibronectin Pre-mRNA

1993 • 13 citations

Selective enzymatic depletion during RNA-PCR: improved detection of rare RNA forms.

1993 • 4 citations