Combinatorial protein design: strategies for screening protein libraries

Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescence resonance energy transfer

1996 • 1,462 citations

Improved Green Fluorescent Protein by Molecular Evolution Using DNA Shuffling

1996 • 1,212 citations

Sorting single molecules: application to diagnostics and evolutionary biotechnology.

1994 • 960 citations

Mutations that suppress the thermosensitivity of green fluorescent protein

1996 • 529 citations

Display of heterologous proteins on the surface of microorganisms: From the screening of combinatorial libraries to live recombinant vaccines

1997 • 511 citations

Directed evolution of a para-nitrobenzyl esterase for aqueous-organic solvents

1996 • 419 citations

Creation of drug-specific herpes simplex virus type 1 thymidine kinase mutants for gene therapy.

1996 • 278 citations

Directed evolution of a fucosidase from a galactosidase by DNA shuffling and screening

1997 • 275 citations

Education reforms struggle against apartheid's legacy

1996 • 275 citations

Directed evolution of subtilisin E in Bacillus subtilis to enhance total activity in aqueous dimethylformamide

1996 • 268 citations

High throughput screening in drug discovery

2006 • 221 citations

Fluorescence correlations, single molecule detection and large number screening Applications in biotechnology

1995 • 215 citations

Chemical Selection for Catalysis in Combinatorial Antibody Libraries

1997 • 208 citations

Fluorescence polarization — a new tool for cell and molecular biology

1995 • 185 citations

The origin of genetic information: viruses as models

1993 • 179 citations

Generation of Large Libraries of Random Mutants in Bacillus subtilis by PCR-Based Plasmid Multimerization

1997 • 162 citations

Strategies for the in vitro evolution of protein function: enzyme evolution by random recombination of improved sequences 1 1Edited by J. Wells

1997 • 159 citations

QuickE. A Fast Spectrophotometric Method To Measure the Enantioselectivity of Hydrolases

1997 • 140 citations

catELISA: a facile general route to catalytic antibodies.

1993 • 133 citations

Directed evolution: Creating biocatalysts for the future

1996 • 119 citations

Engineering new functions and altering existing functions

1996 • 116 citations

Active barnase variants with completely random hydrophobic cores.

1996 • 114 citations

Human Immunodeficiency Virus Reverse Transcriptase

1996 • 112 citations

The role of turns in the structure of an α-helical protein

1993 • 101 citations

Mutation of Pro-258 in transmembrane domain 6 constitutively activates the G protein-coupled alpha-factor receptor.

1996 • 98 citations

Functional and nonfunctional mutations distinguished by random recombination of homologous genes

1997 • 72 citations

Tumor protease-activated, pore-forming toxins from a combinatorial library

1996 • 65 citations

Accurate high-speed liquid handling of very small biological samples.

1993 • 61 citations

Guided Evolution of Enzymes with New Substrate Specificities

1996 • 53 citations

Isolation of Inactive and G Protein-resistant Adenylyl Cyclase Mutants Using Random Mutagenesis

1995 • 49 citations

A Rapid Screen of Active Site Mutants in Glycinamide Ribonucleotide Transformylase

1996 • 47 citations

Novel human DNA alkyltransferases obtained by random substitution and genetic selection in bacteria.

1996 • 45 citations

Monitoring Catalytic Activity by Immunoassay: Implications for Screening

1994 • 43 citations

Combinatorial manipulation of three key active site residues in glycinamide ribonucleotide transformylase

1997 • 38 citations

Sequence replacements in the central β‐turn of plastocyanin

1996 • 36 citations

Random Mutagenesis of the cAMP Chemoattractant Receptor, c/AR1, of Dictyostelium

1997 • 32 citations

Substitutions of Ser for Asn-163 and Pro for Leu-264 Are Important for Stabilization of Lipase from Pseudomonas aeruginosa

1996 • 26 citations

Encoded reaction cassette for the highly sensitive detection of the making and breaking of chemical bonds.

1995 • 22 citations

Selection ofStreptomyces griseusProtease B Mutants With Desired Alterations in Primary Specificity Using a Library Screening Strategy

1996 • 22 citations

[29] Digital imaging spectroscopy for massively parallel screening of mutants

1995 • 22 citations

[3] Phage display of proteases and macromolecular inhibitors

1996 • 21 citations

Screening for Membrane-Permeabilizing Mutants of the Poliovirus Protein 3AB

1996 • 20 citations

Thermostable variants of subtilisin selected by temperature‐gradient gel electrophoresis

1996 • 14 citations

A rapid and effective procedure for screening protease mutants

1996 • 13 citations

In vivo system for the detection of low level activity barnase mutants

1995 • 11 citations

[17] Approaches to high-volume screening assays of viral polymerases and related proteins

1996 • 4 citations

catELISAELISA-based detection of catalytic antibodies and enzymes1

1996 • 1 citations

Deleted Work

1955 • 0 citations