Industrial biocatalysis today and tomorrow

Printing Proteins as Microarrays for High-Throughput Function Determination

2000 • 2,754 citations

Rapid evolution of a protein in vitro by DNA shuffling

1994 • 1,901 citations

Rules for optimization of biocatalysis in organic solvents

1987 • 1,629 citations

DNA shuffling by random fragmentation and reassembly: in vitro recombination for molecular evolution.

1994 • 1,274 citations

Asymmetric transformations catalyzed by enzymes in organic solvents

1990 • 968 citations

Epibatidine: a novel (chloropyridyl)azabicycloheptane with potent analgesic activity from an Ecuadoran poison frog

1992 • 553 citations

Directed evolution of biocatalysts

1999 • 386 citations

Biotransformations in Organic Chemistry

1997 • 349 citations

Biosynthesis and regulation of microbial polyunsaturated fatty acid production

1999 • 325 citations

Biotransformations in Organic Chemistry — A Textbook

1995 • 311 citations

Penicillin Acylase in the Industrial Production of β-Lactam Antibiotics

1998 • 308 citations

Salts dramatically enhance activity of enzymes suspended in organic solvents

1994 • 257 citations

Chiral Compounds Synthesized by Biocatalytic Reductions [New Synthetic Methods (51)]

1985 • 231 citations

Towards a Biocatalyst for ( S )-Styrene Oxide Production: Characterization of the Styrene Degradation Pathway of Pseudomonas sp. Strain VLB120

1998 • 220 citations

Application of in situ product-removal techniques to biocatalytic processes

1999 • 211 citations

Cofactor Regeneration for Enzyme-Catalysed Synthesis

1988 • 200 citations

Inhibitor-induced enzyme activation in organic solvents.

1988 • 188 citations

Optisch aktive Amine durch Lipase-katalysierte Methoxyacetylierung

1997 • 178 citations

Bioconversions of aliphatic compounds byPseudomonas oleovorans in multiphase bioreactors: background and economic potential

1990 • 170 citations

The First Synthetic Application of a Monooxygenase Employing Indirect Electrochemical NADH Regeneration

2001 • 161 citations

Large-scale production of UDP-galactose and globotriose by coupling metabolically engineered bacteria

1998 • 153 citations

Aqueous-Like Activity of .alpha.-Chymotrypsin Dissolved in Nearly Anhydrous Organic Solvents

1994 • 146 citations

Synthesis of optically pure .alpha.-alkylated .alpha.-amino acids and a single-step method for enantiomeric excess determination

1988 • 145 citations

Sequence of thioredoxin reductase from Escherichia coli. Relationship to other flavoprotein disulfide oxidoreductases.

1988 • 137 citations

Production of enantiopure styrene oxide by recombinantEscherichia coli synthesizing a two-component styrene monooxygenase

2000 • 136 citations

On the Salt-Induced Activation of Lyophilized Enzymes in Organic Solvents: Effect of Salt Kosmotropicity on Enzyme Activity

2000 • 130 citations

Biocatalytic plastics as active and stable materials for biotransformations

1997 • 127 citations

In Vitro ATP Regeneration from Polyphosphate and AMP by Polyphosphate:AMP Phosphotransferase and Adenylate Kinase from Acinetobacter johnsonii 210A

2000 • 122 citations

Formate‐Driven, Non‐Enzymatic NAD(P)H Regeneration for the Alcohol Dehydrogenase Catalyzed Stereoselective Reduction of 4‐Phenyl‐2‐butanone

1992 • 110 citations

Optimizing the salt-induced activation of enzymes in organic solvents: Effects of lyophilization time and water content

1999 • 106 citations

Biosynthesis of synthons in two-liquid-phase media

2000 • 105 citations

A direct electrode-driven P450 cycle for biocatalysis

1997 • 104 citations

Activity and Stability of Enzymes Incorporated into Acrylic Polymers

1995 • 104 citations

Pseudomonas oleovorans as a tool in bioconversions of hydrocarbons: growth, morphology and conversion characteristics in different two-phase systems

1983 • 102 citations

[33] Industrial-scale production and application of immobilized glucose isomerase

1987 • 96 citations

Engineering of a Stable Whole-Cell Biocatalyst Capable of ( S )-Styrene Oxide Formation for Continuous Two-Liquid-Phase Applications

1999 • 94 citations

Enzymatic Oxidation of Methyl Groups on Aromatic Heterocycles: A Versatile Method for the Preparation of Heteroaromatic Carboxylic Acids

1992 • 89 citations

Synthesis of Water-Soluble Paclitaxel Derivatives by Enzymatic Acylation

1997 • 88 citations

Combinatorial biocatalysis: a natural approach to drug discovery

1998 • 88 citations

Enzymatic resolution of α,α-disubstituted α-amino acid esters and amides

1993 • 87 citations

Biocatalysts for Industry

1991 • 86 citations

Biocatalytic Solvent-Free Polymerization To Produce High Molecular Weight Polyesters

1997 • 85 citations

An integrated process for the production of toxic catechols from toxic phenols based on a designer biocatalyst

1999 • 81 citations

Enzymatic Hydroxylations in Industrial Application

1991 • 81 citations

Integrated two-liquid phase bioconversion and product-recovery processes for the oxidation of alkanes: Process design and economic evaluation

1999 • 80 citations

Enzyme-catalyzed synthesis of sugar-containing monomers and linear polymers

2000 • 79 citations

Developments toward large-scale bacterial bioprocesses in the presence of bulk amounts of organic solvents

1998 • 78 citations

Bioconversion of N-Octane to Octanoic Acid by a Recombinant Escherichia Coli Cultured in a Two-Liquid Phase Bioreactor

1991 • 78 citations

Whole cell biocatalysis in nonconventional media

1993 • 78 citations

Preparation of (S)-piperazine-2-carboxylic acid, (R)-piperazine-2-carboxylic acid, and (S)-piperidine-2-carboxylic acid by kinetic resolution of the corresponding racemic carboxamides with stereoselective amidases in whole bacterial cells

1997 • 77 citations

Biocatalysis in the gas phase

1993 • 74 citations

Microbial Conversion of Indene to Indandiol: A Key Intermediate in the Synthesis of CRIXIVAN

1999 • 73 citations

Efficient production of optically active styrene epoxides in two-liquid phase cultures

1994 • 67 citations

Preparative scale production of 3-substituted catechols using a novel monooxygenase from Pseudomonas azelaica HBP 1

1998 • 65 citations

Cofactor-Bound Cross-Linked Enzyme Crystals (CLEC) of Alcohol Dehydrogenase

2000 • 64 citations

Application of nitrite converting enzymes for the production of useful compounds

1990 • 64 citations

Quantitative Structure−Activity Relationships for the in Vitro Antimycobacterial Activity of Pyrazinoic Acid Esters

1996 • 59 citations

Synthesis of 1,2-Epoxyoctane by Pseudomonas oleovorans During Growth in a Two-Phase System Containing High Concentrations of 1-Octene

1981 • 57 citations

Practical approach to high-yield enzymic stereospecific organic synthesis in multiphase systems

1981 • 51 citations

Effects of crown ethers and small amounts of cosolvent on the activity and enantioselectivity of α-chymotrypsin in organic sofvents

1996 • 50 citations

Preparation of optically active N-benzyl-3-hydroxypyrrolidine by enzymatic hydroxylation

1999 • 47 citations

Cofactor Regeneration in Biocatalysis in Organic Media

1996 • 46 citations

Induction of catalytic activity in proteins by lyophilization in the presence of a transition state analogue

1998 • 46 citations

Bioconversion of 2-cyanopyrazine to 5-hydroxypyrazine-2-carboxylic acid with Agrobacterium sp. DSM 6336

1997 • 43 citations

Biocatalysis and Process Integration in the Synthesis of Semi-synthetic Antibiotics

1996 • 42 citations

Biokatalysatoren und Enzymtechnologie

1997 • 41 citations

Synthesis of racemic .alpha.-amino carboxamides via Lewis acid-mediated reactions of .alpha.-methoxyglycinamide derivatives with allylsilanes: enzymic resolution to optically active .alpha.-amino acids

1992 • 39 citations

Artificial Electron Carriers for Preparative Biocatalytic Redox Reactions Forming Reversibly Carbon Hydrogen Bonds with Enzymes Present in Strict or Facultative Anaerobes

1995 • 39 citations

Chemical versus biochemical conversion: When and how to use biocatalysts

2000 • 38 citations

Medium chain length alkane solvent-cell transfer rates in two-liquid phase,Pseudomonas oleovorans cultures

1998 • 38 citations

Renewable Functionalized Pyridines Derived from Microbial Metabolites of the Alkaloid (S)-Nicotine

1997 • 37 citations

Biocatalytic combinatorial synthesis

1999 • 33 citations

Application of Enzymes in Industrial Organic Synthesis

1997 • 29 citations

ChemInform Abstract: Optically Active Amines via Lipase‐Catalyzed Methoxyacetylation.

1997 • 26 citations

[20] Cross-linked enzyme crystals of lipases as catalysts for kinetic resolution of acids and alcohols

1997 • 23 citations

Alkanol removal from the apolar phase of a two-liquid phase bioconversion system. Part 1: Comparison of a less volatile and a more volatile in-situ extraction solvent for the separation of 1-octanol by distillation

1998 • 20 citations

Enzymology in monophasic organic media

1992 • 20 citations

Alkanol removal from the apolar phase of a two-liquid phase bioconversion system. Part 2: Effect of fermentation medium on batch distillation

1998 • 14 citations

Immobilized Streptomyces clavuligerus NP1 Cells for Biotransformation of Penicillin G into Deacetoxycephalosporin G

2000 • 13 citations

Screening of novel enzymes for the production of useful compounds

1998 • 12 citations

The Development of Enzymes for the Preparation of Chemicals

1999 • 8 citations

Preparation and use of enantiomerically pure Ca-tetrasubstituted a-amino acids

1996 • 4 citations

Deleted Work

1955 • 0 citations