Structure and function of Factor VIII

Characterization of the human factor VIII gene

1984 • 983 citations

Molecular cloning of a cDNA encoding human antihaemophilic factor

1984 • 937 citations

Structure of human factor VIII

1984 • 898 citations

The role of phospholipids and factor Va in the prothrombinase complex.

1980 • 672 citations

Cell Biology of von Willebrand Factor

1990 • 604 citations

The role of phospholipid and factor VIIIa in the activation of bovine factor X.

1981 • 590 citations

[Changes under the influence of high pressure of certain properties of myosin, actomyosin and actin].

1960 • 499 citations

Complete cDNA and derived amino acid sequence of human factor V.

1987 • 416 citations

Blood coagulation factors V and VIII: structural and functional similarities and their relationship to hemorrhagic and thrombotic disorders

1988 • 405 citations

Proteolytic processing of human factor VIII. Correlation of specific cleavages by thrombin, factor Xa, and activated protein C with activation and inactivation of factor VIII coagulant activity

1986 • 404 citations

Synthesis, processing, and secretion of recombinant human factor VIII expressed in mammalian cells.

1988 • 385 citations

The relationship of N-linked glycosylation and heavy chain-binding protein association with the secretion of glycoproteins.

1987 • 357 citations

A large region (approximately equal to 95 kDa) of human factor VIII is dispensable for in vitro procoagulant activity.

1986 • 314 citations

Membrane asymmetry and blood coagulation

1977 • 309 citations

Sulfation of Tyr1680 of human blood coagulation factor VIII is essential for the interaction of factor VIII with von Willebrand factor.

1991 • 275 citations

Construction and characterization of an active factor VIII variant lacking the central one-third of the molecule

1986 • 275 citations

A major factor VIII binding domain resides within the amino-terminal 272 amino acid residues of von Willebrand factor.

1987 • 263 citations

Inactivation of human factor VIII by activated protein C. Cofactor activity of protein S and protective effect of von Willebrand factor.

1988 • 244 citations

Association of the factor VIII light chain with von Willebrand factor.

1988 • 223 citations

Human factor VIIIa subunit structure. Reconstruction of factor VIIIa from the isolated A1/A3-C1-C2 dimer and A2 subunit

1991 • 217 citations

A Factor VIII Neutralizing Monoclonal Antibody and a Human Inhibitor Alloantibody Recognizing Epitopes in the C2 Domain Inhibit Factor VIII Binding to von Willebrand Factor and to Phosphatidylserine

1993 • 199 citations

Synthetic factor VIII peptides with amino acid sequences contained within the C2 domain of factor VIII inhibit factor VIII binding to phosphatidylserine

1990 • 198 citations

Substructure of human von Willebrand factor.

1985 • 198 citations

Antihemophilic Factor: Separation of an Active Fragment Following Dissociation by Salts or Detergents

1972 • 193 citations

Activated protein C-catalyzed inactivation of human factor VIII and factor VIIIa. Identification of cleavage sites and correlation of proteolysis with cofactor activity.

1991 • 189 citations

Isolation and characterization of single chain bovine factor V.

1979 • 184 citations

Molecular basis of factor VIII inhibition by human antibodies. Antibodies that bind to the factor VIII light chain prevent the interaction of factor VIII with phospholipid.

1989 • 183 citations

Response to infusions of polyelectrolyte fractionated human factor VIII concentrate in human haemophilia A and von Willebrand's disease

1982 • 180 citations

Monoclonal antibodies to porcine factor VIII coagulant and their use in the isolation of active coagulant protein

1982 • 178 citations

Purified human factor VIII procoagulant protein: comparative hemostatic response after infusions into hemophilic and von Willebrand disease dogs.

1985 • 175 citations

The active site of blood coagulation factor Xa. Its distance from the phospholipid surface and its conformational sensitivity to components of the prothrombinase complex.

1987 • 170 citations

Proteolytic requirements for thrombin activation of anti-hemophilic factor (factor VIII).

1988 • 170 citations

The size of human factor VIII heterodimers and the effects produced by thrombin

1986 • 161 citations

Anti-factor VIII antibodies of hemophiliac patients are frequently directed towards nonfunctional determinants and do not exhibit isotypic restriction

1993 • 155 citations

Von Willebrand Factor: Dissociation from Antihemophilic Factor Procoagulant Activity

1973 • 150 citations

pH-dependent denaturation of thrombin-activated porcine factor VIII.

1990 • 149 citations

The interaction between human blood-coagulation factor VIII and von Willebrand factor. Characterization of a high-affinity binding site on factor VIII

1989 • 149 citations

Subunit structure of thrombin-activated porcine factor VIII

1989 • 147 citations

Binding of human factor VIII to phospholipid vesicles.

1990 • 142 citations

Isolation and characterization of human factor VIII: molecular forms in commercial factor VIII concentrate, cryoprecipitate, and plasma.

1986 • 140 citations

An immunogenic region within residues Val1670-Glu1684 of the factor VIII light chain induces antibodies which inhibit binding of factor VIII to von Willebrand factor.

1988 • 136 citations

Loss of prothrombin and of factor Xa-factor Va interactions upon inactivation of factor Va by activated protein C.

1984 • 133 citations

Structural basis for the decreased procoagulant activity of human factor VIII compared to the porcine homolog

1991 • 126 citations

von Willebrand factor mediates protection of factor VIII from activated protein C-catalyzed inactivation

1991 • 125 citations

Epitope mapping of human factor VIII inhibitor antibodies by deletion analysis of factor VIII fragments expressed in Escherichia coli.

1988 • 122 citations

Coagulant properties of hybrid human/porcine factor VIII molecules.

1992 • 121 citations

Human von Willebrand factor: a multivalent protein composed of identical subunits

1986 • 120 citations

Stabilization of thrombin-activated porcine factor VIII:C by factor IXa phospholipid

1984 • 117 citations

Activation of porcine factor VIII:C by thrombin and factor Xa

1985 • 114 citations

Molecular genetics of von Willebrand disease

1992 • 113 citations

Factor VIII inhibitors

1985 • 109 citations

Molecular Forms of Antihaemophilic Globulin in Plasma, Cryoprecipitate and after Thrombin Activation

1970 • 108 citations

The active site of factor IXa is located far above the membrane surface and its conformation is altered upon association with factor VIIIa. A fluorescence study.

1992 • 105 citations

Interaction of factor VIII-von Willebrand Factor with phospholipid vesicles

1981 • 104 citations

Native conformation of human von Willebrand protein. Analysis by electron microscopy and quasi-elastic light scattering.

1985 • 102 citations

Identification and functional importance of tyrosine sulfate residues within recombinant factor VIII

1992 • 100 citations

Reaction Sequence of Blood Coagulation

1967 • 97 citations

Specific membrane binding of factor VIII is mediated by O-phospho-L-serine, a moiety of phosphatidylserine

1993 • 94 citations

Substructure of human von Willebrand factor. Proteolysis by V8 and characterization of two functional domains.

1986 • 94 citations

The effect of plasma von Willebrand factor on the binding of human factor VIII to thrombin-activated human platelets.

1991 • 93 citations

Differential proteolytic activation of factor VIII-von Willebrand factor complex by thrombin.

1989 • 92 citations

Porcine Willebrand factor: a population of multimers.

1978 • 91 citations

The effect of von Willebrand factor on activation of factor VIII by factor Xa

1990 • 90 citations

Specificity of phosphatidylserine-containing membrane binding sites for factor VIII. Studies with model membranes supported by glass microspheres (lipospheres).

1992 • 88 citations

Identification of the binding site for activated protein C on the light chain of factors V and VIII.

1990 • 88 citations

Binding of factor VIIIa and factor VIII to factor IXa on phospholipid vesicles.

1992 • 87 citations

von Willebrand factor is a cofactor for thrombin-catalyzed cleavage of the factor VIII light chain.

1990 • 85 citations

Molecular genetics of von Willebrand disease

1992 • 85 citations

Sequence of the Murine Factor VIII cDNA

1993 • 84 citations

Inhibition of human factor VIIIa by anti-A2 subunit antibodies.

1994 • 83 citations

Characterization of recombinant human factor VIII.

1987 • 76 citations

A monoclonal antibody to von Willebrand factor (vWF) inhibits factor VIII binding. Localization of its antigenic determinant to a nonadecapeptide at the amino terminus of the mature vWF polypeptide.

1989 • 72 citations

Interaction of clotting factor V heavy chain with prothrombin and prethrombin 1 and role of activated protein C in regulating this interaction: analysis by analytical ultracentrifugation

1989 • 71 citations

Human factor VIII procoagulant activity and phospholipid interaction

1981 • 67 citations

Isolation and characterization of thrombin-activated human factor VIII.

1994 • 66 citations

The activation and inactivation of human factor VIII by thrombin: effect of inhibitors of thrombin

1981 • 66 citations

Inhibition of human coagulation factor VIII by monoclonal antibodies. Mapping of functional epitopes with the use of recombinant factor VIII fragments

1989 • 65 citations

Electron microscopy of human factor VIII/Von Willebrand glycoprotein: effect of reducing reagents on structure and function.

1982 • 64 citations

The effect of thrombin on the complex between factor VIII and von Willebrand factor

1987 • 63 citations

A recombinant factor VIII A2 domain polypeptide quantitatively neutralizes human inhibitor antibodies that bind to A2

1993 • 62 citations

Stoichiometry of the porcine factor VIII-von Willebrand factor association.

1987 • 59 citations

Intrinsic pathway activation of factor X and its activation peptide-deficient derivative, factor Xdes-143-191.

1992 • 58 citations

Proteolytic interactions of factor IXa with human factor VIII and factor VIIIa

1992 • 58 citations

Inhibition of activated porcine Factor IX by dansyl-glutamyl-glycyl-arginyl-chloromethylketone

1984 • 55 citations

Role of the COOH-terminal acidic region of A1 subunit in A2 subunit retention in human factor VIIIa.

1993 • 54 citations

Effects of thrombin treatment of preparations of factor VIII and the Ca2+-dissociated small active fragment.

1975 • 54 citations

Inactivation of factor VIII by factor IXa

1992 • 50 citations

Factor IXa enhances reconstitution of factor VIIIa from isolated A2 subunit and A1/A3-C1-C2 dimer.

1992 • 46 citations

Molecular characterization of commercial porcine factor VIII concentrate

1988 • 45 citations

The interaction of rDNA factor VIII, factor VIIIdes-797-1562 and factor VIIIdes-797-1562-derived peptides with phospholipid

1987 • 39 citations

Electron microscopy of human factor V and factor VIII: correlation of morphology with domain structure and localization of factor V activation fragments.

1990 • 37 citations

The In Vitro Association of Antihemophilic Factor and von Willebrand Factor

1983 • 35 citations

Purification and characterization of factor VIII 372-Cys: a hypofunctional cofactor from a patient with moderately severe hemophilia A

1990 • 32 citations

Inhibition by heparin of thrombin-catalyzed activation of the factor VIII-von Willebrand factor complex.

1994 • 29 citations

Thrombin-activated and factor Xa-activated human factor VIII: Differences in cofactor activity and decay rate

1992 • 29 citations

Factor IXa and von Willebrand factor modify the inactivation of factor VIII by activated protein C.

1990 • 26 citations

A monoclonal antibody to factor VIII inhibits von Willebrand factor binding and thrombin cleavage

1991 • 26 citations

Structural model of porcine factor VIII and factor VIIIa molecules based on scanning transmission electron microscope (STEM) images and STEM mass analysis.

1990 • 26 citations

Epitope mapping of human factor VIII inhibitor antibodies by site-directed mutagenesis of a factor VIII polypeptide

1992 • 22 citations

Factor VIII-East Hartford (arginine 1689 to cysteine) has procoagulant activity when separated from von Willebrand factor.

1992 • 18 citations