Novel vectors for the expression of antibody molecules using variable regions generated by polymerase chain reaction

Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction

1987 • 64,270 citations

Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction

1987 • 45,673 citations

Site-directed mutagenesis by overlap extension using the polymerase chain reaction

1989 • 7,496 citations

Sequences of proteins of immunological interest

1984 • 3,427 citations

Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter.

1982 • 2,753 citations

Possible role of flanking nucleotides in recognition of the AUG initiator codon by eukaryotic ribosomes

1981 • 1,265 citations

Three-Dimensional Structure of an Antigen-Antibody Complex at 2.8 Å Resolution

1986 • 1,173 citations

Selection for animal cells that express the Escherichia coli gene coding for xanthine-guanine phosphoribosyltransferase.

1981 • 1,048 citations

Chimeric human antibody molecules: mouse antigen-binding domains with human constant region domains.

1984 • 1,002 citations

Cloning immunoglobulin variable domains for expression by the polymerase chain reaction.

1989 • 712 citations

Studies of aglycosylated chimeric mouse-human IgG. Role of carbohydrate in the structure and effector functions mediated by the human IgG constant region.

1989 • 519 citations

A simple and efficient method for direct cloning of PCR products using ddT-tailed vectors

1991 • 381 citations

Chimeric mouse-human IgG1 antibody that can mediate lysis of cancer cells.

1987 • 204 citations

Transfectomas Provide Novel Chimeric Antibodies

1985 • 184 citations

Two dominant-acting selectable markers for gene transfer studies in mammalian cells.

1988 • 184 citations

Rapid cloning of rearranged immunoglobulin genes from human hybridoma cells using mixed primers and the polymerase chain reaction

1989 • 172 citations

High-level expression of chimeric antibodies using adapted cDNA variable region cassettes

1989 • 158 citations

A rapid method of cloning functional variable-region antibody genes in Escherichia coli as single-chain immunotoxins.

1990 • 156 citations

Glycosylation of a VH residue of a monoclonal antibody against alpha (1----6) dextran increases its affinity for antigen.

1988 • 135 citations

Hybridoma fusion cell lines contain an aberrant kappa transcript

1988 • 126 citations

The intron requirement for immunoglobulin gene expression is dependent upon the promoter

1988 • 87 citations

[32] Production and properties of chimeric antibody molecules

1989 • 85 citations

Variable region framework differences result in decreased or increased affinity of variant anti-digoxin antibodies.

1988 • 59 citations

Significant structural and functional change of an antigen-binding site by a distant amino acid substitution: proposal of a structural mechanism.

1989 • 57 citations

Cloning and sequencing of imniunoglobulin variable-region genes using degenerate oligodeoxy-ribonucleotides and polymerase chain reaction

1989 • 50 citations

Nucleotide sequence of theEsherichia colixanthine-guanine phosphoribosyl transferase gene

1983 • 46 citations

Primer design for the cloning of immunoglobulin heavy-chain leader-variable regions from mouse hybridoma cells using the PCR.

1991 • 46 citations

Anti-Idiotype Monoclonal Antibody Carrying the Internal Image of Ganglioside GM3

1990 • 44 citations

Specific Amplification of Rearranged Immunoglobulin Variable Region Genes from Mouse Hybridoma Cells

1990 • 43 citations

Expression of Human Anti-Tetanus Toxoid Antibody in Transfected Murine Myeloma Cells

1989 • 39 citations

Production of murine V-human cr1 chimeric anti-tag72 antibody using V region cdna amplified by PCR

1990 • 29 citations