A rapid method of cloning functional variable-region antibody genes in Escherichia coli as single-chain immunotoxins.

Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase

1988 • 17,059 citations

Enzymatic Amplification of β-Globin Genomic Sequences and Restriction Site Analysis for Diagnosis of Sickle Cell Anemia

1985 • 9,128 citations

Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes

1986 • 6,284 citations

Reshaping human antibodies for therapy

1988 • 1,555 citations

Protein engineering of antibody binding sites: recovery of specific activity in an anti-digoxin single-chain Fv analogue produced in Escherichia coli.

1988 • 1,536 citations

Single-Chain Antigen-Binding Proteins

1988 • 1,450 citations

Replacing the complementarity-determining regions in a human antibody with those from a mouse

1986 • 1,356 citations

Assembly of a Functional Immunoglobulin F _v Fragment in Escherichia coli

1988 • 973 citations

Direct Cloning and Sequence Analysis of Enzymatically Amplified Genomic Sequences

1986 • 766 citations

Cloning immunoglobulin variable domains for expression by the polymerase chain reaction.

1989 • 712 citations

Escherichia coli Secretion of an Active Chimeric Antibody Fragment

1988 • 575 citations

A recombinant immunotoxin consisting of two antibody variable domains fused to Pseudomonas exotoxin

1989 • 420 citations

Reshaping Human Antibodies: Grafting an Antilysozyme Activity

1988 • 348 citations

Redesigning Nature's Poisons to Create Anti-Tumor Reagents

1987 • 329 citations

Selective killing of HIV-infected cells by recombinant human CD4-Pseudomonas exotoxin hybrid protein

1988 • 253 citations

Immunotoxins

1986 • 230 citations

Cloning of the immunological repertoire in Escherichia coli for generation of monoclonal catalytic antibodies: construction of a heavy chain variable region-specific cDNA library.

1989 • 201 citations

Activity of immunotoxins constructed with modified Pseudomonas exotoxin A lacking the cell recognition domain.

1988 • 147 citations

Pseudomonas exotoxin: chimeric toxins

1989 • 129 citations

Domain II Mutants of Pseudomonas Exotoxin Deficient in Translocation

1989 • 93 citations

Anti-transferrin receptor antibody linked to Pseudomonas exotoxin as a model immunotoxin in human ovarian carcinoma cell lines.

1985 • 91 citations

Cytotoxic activity of an interleukin 6-Pseudomonas exotoxin fusion protein on human myeloma cells.

1988 • 82 citations

Cytotoxic activities of a fusion protein comprised of TGFα and Pseudomonas exotoxin

1989 • 71 citations

Pseudomonas exotoxin coupled to a monoclonal antibody against ovarian cancer inhibits the growth of human ovarian cancer cells in a mouse model.

1987 • 64 citations

Direct cDNA cloning of the rearranged immunoglobulin variable region.

1989 • 63 citations