Distinct domains of an oligotopic membrane protein are Sec-dependent and Sec-independent for membrane insertion.

Molecular Cloning. A Laboratory Manual

1983 • 27,328 citations

Construction of Biologically Functional Bacterial Plasmids In Vitro

1973 • 1,699 citations

Mechanism of Assembly of the Outer Membrane of Salmonella typhimurium

1972 • 1,574 citations

Two differentially regulated mRNAs with different 5′ ends encode secreted and intracellular forms of yeast invertase

1982 • 1,539 citations

Intracellular protein topogenesis

1980 • 1,378 citations

[32] Oligonucleotide-directed mutagenesis of DNA fragments cloned into M13 vectors

1983 • 1,093 citations

Multiple Mechanisms of Protein Insertion into and Across Membranes

1985 • 720 citations

Topogenic signals in integral membrane proteins

1988 • 715 citations

A Genetic Approach to Analyzing Membrane Protein Topology

1986 • 610 citations

Control of topology and mode of assembly of a polytopic membrane protein by positively charged residues

1989 • 587 citations

Protein Translocation Across Membranes

1988 • 482 citations

Correlation of competence for export with lack of tertiary structure of the mature species: A study in vivo of maltose-binding protein in E. coli

1986 • 473 citations

Mechanism of Incorporation of Cell Envelope Proteins in Escherichia Coli

1982 • 344 citations

Sequence of the leader peptidase gene of Escherichia coli and the orientation of leader peptidase in the bacterial envelope.

1983 • 307 citations

Use of gene fusion to study secretion of maltose-binding protein into Escherichia coli periplasm

1979 • 267 citations

Fine-tuning the topology of a polytopic membrane protein: Role of positively and negatively charged amino acids

1990 • 221 citations

Effects of two sec genes on protein assembly into the plasma membrane of Escherichia coli.

1985 • 217 citations

An artificial anchor domain: hydrophobicity suffices to stop transfer

1985 • 206 citations

Synthesis, assembly into the cytoplasmic membrane, and proteolytic processing of the precursor of coliphage M13 coat protein.

1980 • 200 citations

The isolation of homogeneous leader peptidase from a strain of Escherichia coli which overproduces the enzyme.

1982 • 179 citations

Insertion of proteins into bacterial membranes: mechanism, characteristics, and comparisons with the eucaryotic process

1989 • 167 citations

Mechanisms of integration of de novo-synthesized polypeptides into membranes: signal-recognition particle is required for integration into microsomal membranes of calcium ATPase and of lens MP26 but not of cytochrome b5.

1983 • 155 citations

Signals for the incorporation and orientation of cytochrome P450 in the endoplasmic reticulum membrane.

1988 • 151 citations

Fine structure of a membrane anchor domain

1985 • 151 citations

Positively charged residues are important determinants of membrane protein topology

1990 • 132 citations

High-level expression of M13 gene II protein from an inducible polycistronic messenger RNA

1985 • 130 citations

Energetics and intermediates of the assembly of Protein OmpA into the outer membrane of Escherichia coli.

1983 • 114 citations

Use of phoA fusions to study the topology of the Escherichia coli inner membrane protein leader peptidase

1989 • 111 citations

Mutations altering the cellular localization of the phage lambda receptor, an Escherichia coli outer membrane protein.

1978 • 106 citations

A small hydrophobic domain anchors leader peptidase to the cytoplasmic membrane of Escherichia coli.

1987 • 90 citations

Bacterial leader peptidase, a membrane protein without a leader peptide, uses the same export pathway as pre-secretory proteins

1984 • 84 citations

Integration of a small integral membrane protein, M2, of influenza virus into the endoplasmic reticulum: analysis of the internal signal-anchor domain of a protein with an ectoplasmic NH2 terminus.

1988 • 77 citations

The Function of a Leader Peptide in Translocating Charged Amino Acyl Residues Across a Membrane

1990 • 73 citations

Leader Peptidase of Escherichia coli : Critical Role of a Small Domain in Membrane Assembly

1987 • 72 citations

The role of the polar, carboxyl-terminal domain of Escherichia coli leader peptidase in its translocation across the plasma membrane.

1986 • 68 citations

Efficient translocation of positively charged residues of M13 procoat protein across the membrane excludes electrophoresis as the primary force for membrane insertion.

1990 • 63 citations

The cytoplasmic domain of Escherichia coli leader peptidase is a "translocation poison" sequence.

1988 • 61 citations

Positive charges in the cytoplasmic domain of Escherichia coli leader peptidase prevent an apolar domain from functioning as a signal.

1989 • 48 citations

The internal signal sequence of Escherichia coli leader peptidase is necessary, but not sufficient, for its rapid membrane assembly.

1987 • 47 citations

Genetic Studies on Protein Export in Bacteria

1986 • 45 citations

Deletion analysis of the internal signal-anchor domain of the human asialoglycoprotein receptor H1.

1987 • 40 citations

Both a short hydrophobic domain and a carboxyl-terminal hydrophilic region are important for signal function in the Escherichia coli leader peptidase

1989 • 30 citations

Characterization of the internal signal-anchor domain of Escherichia coli leader peptidase.

1988 • 21 citations

Chapter 2 In Vivo Protein Translocation into or across the Bacterial Plasma Membrane

1991 • 4 citations